193 results about "Emasculation" patented technology

The invention relates to the technical field of biological engineering, and relates to a creation method and a purpose of a paddy rice male sterile line. The creation method is that: (a) with a common biological engineering method, a nucleotide sequence of the amino acid coded as SEQ ID NO.3 is knocked out or changed, such that the amino acid sequence is subjected to deletion or mutation, and theactivity level of the polypeptide corresponding to the amino acid sequence is reduced or lost; or (b) with a common biological engineering method, the expression of the nucleotide sequence of the amino acid sequence coded as SEQ ID NO.3 is suppressed or reduced, and the expression level of the polypeptide corresponding to the amino acid sequence is reduced. During a vegetative growth stage, the paddy rice mutant obtained by the invention has no abnormality. However, a homozygote plant is completely sterile. When the paddy rice mutant is applied in hybridization, the work of female parent emasculation can be eliminated, such that production efficiency is substantially improved, and labor cost is reduced. Therefore, the paddy rice male sterile line provided by the invention has important application in agricultural productions.

The invention discloses a seed production method for a hybrid pepper variety. The seed production method comprises the following steps of: manually removing an anther of a pepper female parent, collecting pollen of a peeper male parent, and carrying out artificial supplementary pollination on the pepper female parent, wherein the produced F1 generation seed is a hybrid seed; the step of manually removing the anther of the pepper female parent is carried out through adopting a manual rotation method; the manual rotation method comprises the following steps: a joint position between a pedicel and a receptacle is pinched by a left hand; a position, where is close to the receptacle, of a corolla is gently pinched by a thumb and a forefinger of a right hand; and the thumb and the forefinger slowly twist, so that the corolla and the anther are removed, and a style and an ovary are exposed. A female parent emasculation method (manual rotation method) disclosed by the invention has the characteristics of saving labor and force, capably reducing production cost, realizing thorough emasculation, and capably ensuring emasculation quality; and a stamp-pad ink marking method is adopted by the invention, and the stamp-pad ink cost is low, so that the production cost can be reduced, as well as labor and force are saved. The conventional marking method is an iron ring marking method; the ironring marking method is realized through bonding an iron ring on a pollinated stalk, so that the operation cost is high; and the iron ring has a certain weight, so that flowers are easy to fall off, therefore the yield is low.

The invention discloses a method for preservation of pure state of hybrid plants by chemical emasculation. In the method, a breed sensitive to acetolactate synthase inhibitory type herbicide is selected as female parent and a breed resistant to acetolactate synthase inhibitory type herbicide is selected as male parent, acetolactate synthase inhibitory type herbicide with activity of chemical hybridizing agent is used for processing the female parent to obtain male sterility, the male parent pollinates the female parent, the seeds of the female parent line are harvested to be cross breeds, the obtained F1 generation cross breed has herbicide resistance, after the F1 generation cross breed is planted, and acetolactate synthase inhibitory type herbicide seedling stage processing is used for removing uncrossed female parent seedling, so as to remain real cross breed. The method utilizes herbicide for preservation of pure state while realizing weeding. The invention has more advantages than common breed in the aspect of adapted breed simplified culture requirement.

A method for production of sweet pepper hybrid seeds includes: A, making a male-female parent seedling bed; B, seeding for male and female parents at different stages, selecting female parent seeds from original Aidan 92-3-2 produced by Beijing seed company, and selecting male parent seeds from a self-selected yellow-shelled green pepper line 9808-8 produced by Deqing seeds and seedlings Co, Ltd.in Cixi city; C, transplanting seedlings into pots; D, performing field transplanting, and covering with black mulch to prevent grass damage; E, preparing before seed production, paring a land for weed removal once in April, and removing all first-generation flowers and fruits in early May when male parents of the sweet pepper blossom to bear fruits; F, collecting pollen of female parents; G, performing emasculation and pollination for the male parents; H, performing management after seed production, topping after five generations of fruit setting, applying compound fertilizer 20kg per mu by drip irrigation, and controlling greenhouse temperature to be between 22 DEG C and 28 DEG C; and I, harvesting. The sweet pepper seeds produced by the method are high in purity and good in quality, and benefits of per mu yield of sweet pepper planting can be improved greatly.

The invention discloses a breeding method of a yellow bud marker photosensitive male sterile line. The breeding method provided by the invention comprises the following step: performing autocopuation on the yellow bud marker photosensitive male sterile line under the condition of being within 11 hours and 12 hours and 20 minutes so as to get seeds of the yellow bud marker photosensitive male sterile line. The yellow bud marker photosensitive male sterile line is the sterile line, is also a maintainer line of the sterile line, and further has the characteristics of a wide range of restoration sources, free assembly, simple seed production procedure and low seed production cost. The yellow bud marker photosensitive male sterile line comprises a yellow bud marker, hybrid seeds can be effectively removed by screening markers during the seedling stage and photosensitive sterile strains can be further determined. The yellow bud marker photosensitive male sterile line can provide seed resources for breeding of the new photosensitive sterile lines, greatly reduce the cost of artificial emasculation, assemble a large number of hybrid combinations, breed the hybrid seeds and accelerate the breeding process.

The invention discloses two polyploid centella asiatica varieties and a cultivation method thereof. The cultivation method comprises the following steps of 1, selecting terminal buds of a diploid centella asiatica adult plant, and carrying out breeding of a tissue cultured seedling or callus tissue, 2, carrying out chromosome doubling induction culture and differentiation culture of tissue subcultured seedling or callus tissue by oryzalin, 3, cutting down novel buds obtained by the differentiation culture and carrying out cluster bud culture, 4, carrying out chromosome doubling identification of the cultured complete plants having roots, stems and leaves, screening the tetraploid plant, and planting the tetraploid plant to obtain the tetraploid centella asiatica variety, 5, carrying out artificial emasculation and pollination hybridization of the tetraploid plant and the diploid plant to obtain hybrid seeds (female parent 4N*male parent 2N or male parent 4N* female parent 2N, wherein F1 is 3x), and 6, breeding the hybrid seeds into complete plants, planting the plants, carrying out chromosome number detection and screening the triploid centella asiatica variety.

The invention relates to a plant breeding and reproduction method, and discloses a Mimai No.7 wheat cross-breeding and reproduction method. The method comprises the steps that: (1) parents are selected, wherein a branched wheat Mimai No.4 is adopted as a female parent, and a branched wheat Mimai No.6 is adopted as a male parent, and sexual hybridization is carried out; (2) emasculation and pollination are carried out, wherein anthers of the female parents are removed in one time; stigmas are mature after 1-4 days, and pollination can be carried out; immature embryos are peeled 18-21 days after pollination, and isolated culture is carried out; and generation-adding breeding and system selection are carried out; (3) generation-adding breeding and system selection are carried out, wherein jarovisation treatment is carried out by using greenhouse in winter or refrigerator in summer; through multiple times of selection, branched hybridized wheat novel breed which is Mimai No.7 with good comprehensive performance is obtained on the basis of a second generation. The breed has the advantages of good lodging resistance, good disease resistance, high yield, and good stability of Mimai No.4, and has characteristics of vigorous growth and good stress resistance of the male parent Mimai No.6. Yield increase can be higher than 20% per mu.

The invention discloses a method for breeding multiple generations of paddy rice in one year. The method comprises the following steps: taking paddy rice seeds, breaking dormancy, accelerating germination and then carrying out direct seeding; after paddy rice enters a flowering stage, carrying out glume cutting, emasculation and pollination on a female parent; selecting seed-grain or brown rice matured at earliest time as a next-generation parent in a filling and ripening stage; and repeating the previous three steps until multiple generations of the paddy rice are obtained. According to the invention, the method employs breeding and cultivation manners like direct seeding culture, glume cutting and emasculation, germination acceleration and seeding of brown rice, and rapid dormancy breaking and germination acceleration via seed soaking with a nitric acid solution, so the whole growth period of paddy rice is shortened; and thus, breeding process is shortened, breeding of multiple generations of paddy rice in one year is realized, and the problem of a long breeding period in the prior art is overcome.

The invention specifically relates to application of a male-sterility gene OsGEN and a method for restoring fertility, belonging to the technical field of rice breeding. The male-sterility gene OsGEN has an amino acid sequence as shown in SEQ ID No. 1. The application of the male-sterility gene OsGEN is that the OsGEN gene is knocked out, changed or inhibited by using a conventional method or based on a CRISPR/Cas9 system so as to allow the expression level of the OsGEN gene in a conventional rice variety to be decreased, and thus, a male-sterility strain of rice is obtained. The invention also relates to the method for restoring male sterility of rice caused by deletion of the OsGEN gene. According to the method, the OsGEN gene is amplified through primers, a mutant plant is transformed by using a genetic transformation means, so the mutant is restored to the phenotype of a wild type. The rice mutant obtained in the invention is free of abnormity in the phase of vegetative growth; however, a homozygote plant is completely infertile. When the mutant is applied to cross breeding, the operation of emasculation of a female parent can be omitted; production efficiency is substantially improved; labor cost is reduced; and the mutant has important application value in agricultural production.

The present invention discloses a submerged rice hybridization method. It includes the steps of emasculation, pollination and returning into field. Said invention adopts CO2 gas to treat rice ear, and utilizes vacuum pump to such and remove pesticide, and makes them be organically combined together, so that it can raise technical content of submerged rice hybridization method, reduce its hybridization work difficulty and raise its working effect.

The invention discloses an artificial emasculation and hybrid control pollination method of robinia pseudoacacia L.. The method comprises the following steps of pollenator making, selection of hybrid male parent and female parent plants, pollen collecting, artificial pollination, pollinated female plant management and hybrid ripe pod harvesting. According to the artificial emasculation and hybrid control pollination method of the robinia pseudoacacia L., the technical steps of emasculation and pollination for the robinia pseudoacacia L. are described in detail, petals and pistil are not damaged, the artificial pollination ripening rate of the robinia pseudoacacia L. can be significantly increased, and the artificial hybridization breeding success rate of the robinia pseudoacacia L. is increased.

The invention provides rice alpha-amylase and application of coding genes of the rice alpha-amylase in pollen fertility control and belongs to the field of biotechnology. The method comprises the following steps: by cloning a nucleotide sequence of Nipponbare rice alpha-amylase, constructing a recombinant expression vector and performing genetic transformation. Under drive of a PG47 promoter, alpha-amylase is specifically expressed at the later pollen development stage; and meanwhile, the alpha-amylase is capable of degrading starch in pollen grains to cause transgenic pollen sterility of therice under guidance of a BT1 transfer signal peptide, the accuracy is high, and the condition that transgenic elements of transgenic crops are spread to other crop varieties by virtue of pollens is effectively avoided. The alpha-amylase disclosed by the invention can be used for maintaining a homozygous recessive state of male sterility plants, so that the artificial emasculation step is saved inthe hybrid seed production process, extensive propagation of sterile lines can be realized, and the labor input and the influence on yield can be reduced, so that the rice alpha-amylase has wide application prospects in the aspects of crop planting resource improvement, genetic breeding and the like.

The invention relates to an intelligentized and multi-functional robot for plant protection. The intelligentized and multi-functional robot for plant protection comprises a control member and a vehicle frame body. The control component is used for realizing selection and automatic control of working modes of the robot for plant protection. The vehicle frame body can be connected with multiple groups of working members. A quick coupling device is arranged among all the working members and the vehicle frame body and used for realizing quick replacement among all the working members and the vehicle frame body. The intelligentized and multi-functional robot for plant protection has following beneficial effects: the intelligentized and multi-functional robot for plant protection can be used for multiple purposes and has integrated functions of fertilizer application, sowing, spraying, emasculation by topping; the intelligentized and multi-functional robot is high in intelligent degree and has functions of remote control, image monitoring, wireless transmission and acquisition of field data; manless driving can be realized; labor cost is greatly decreased; and high operation comfortable and high working precision and 99.9% accuracy are obtained.

The invention relates to a date tree manual control crossbreeding method assisted by molecule, that is, the hybrid progeny of the date tree is obtained by artificially selecting parent, cover net control hybridation, pollination assisted by insects, molecular marker hybrid identification, etc. According to target breeding, the parent is selected, two parents are adjacently planted or grafted on the same date tree; before the plants of the parents blossom out, a net that has the functions of ventilation and light transmission but can stop pollination insects from entering is used for completely covering a parent tree, the pollination insects that never collect pollen are put in the net for helping pollination, and the pollination insects and the net cover are removed after florescence is over; the fruit born by the two parents are all picked up, the kernels are taken out and sowed to obtain seedlings; the genome DNAs of all the seedlings and the two parents are extracted, the molecular marker techniques such as AFLP, etc., are adopted for hybrid identification; the seedlings of the hybrid progeny are systematically observed and evaluated for breeding improved varieties. The method is applied to the practices of date tree breeding, can break through the bottleneck of difficult emasculation in the crossbreeding of date trees and improve the idioplasm innovation and crossbreeding efficiency of the date trees.

The invention provides a rice hybridization and castration method, belongs to the technical field of crop breeding, and particularly relates to a novel rice hybridization and emasculation-water spraying-castration method. The rice hybridization and castration method comprises the following steps: 1, selection of a reasonable sowing date; 2, selection of rice plants; 3, pruning of rice ears; 4, emasculation, water spraying and castration; 5, timely and efficient pollination. Through the adoption of the rice hybridization and castration method, the problem that the conventional rice hybridization work fails due to high labor intensity, incomplete castration and damage to pistil is solved; the rice hybridization and castration method has the advantages that the labor consumption and the cost are reduced, the operation is simple and convenient, the hybridization success rate is high, and the economical and environment-friendly effect is achieved, is deeply welcomed by scientific research breeding workers, and has a broad application prospect.

The invention belongs to the field of rapeseed breeding, and particularly relates to a cultivating method for a high-oleic acid rapeseed variety. The cultivating method is characterized by comprising the following steps: performing hybridization by taking first Brassica napus L. A177 with the oleic acid content of 65 percent or below as a female parent and taking first high-oleic acid Brassica napus L. H003 which has the preservation number of CCTCC NO:P201604 and has the oleic acid content of at least 78 percent as a male parent, and obtaining F1 through artificial emasculation; planting the F1, obtaining a separated DH line through microspores culture; performing high-oleic acid molecular marker identification on strain seedling stage of a DH line group, and determining the genotype of each strain; measuring the content of oleic acid of each single plant by utilizing a gas chromatograph, and determining the content of the oleic acid of the each primarily selected line; selecting the parent strains of which the characters, such as the whole plant pod number, the seed number per pod, the thousand seed weight and the yield per plant, are superior to the parent strain with the oleic acid content lower than 69 percent from the DH strain with the oleic acid content of greater than 75 percent to obtain the high-oleic acid rapeseed selected line and variety of which the oleic acid is greater than 78 percent.

The invention discloses a binary hybridization method of quinoa and relates to the technical field of crop breeding. The binary hybridization method solves the problem that the quinoa hybridization has strong labor intensity, incomplete castration and pistil damage causing hybridization failure. The method comprises: selecting female and male plants needing hybridization, and carrying out binary hybridization on quinoa through an artificial pollination method, wherein the flower of the female plant used by binary hybridization has a pistil mature in advance of a stamen and is fully opened whenthe pistil is mature and the stamen is immaturate and the pistil is fully exposed. The specific quinoa female plant can break through the trouble of the complicated artificial emasculation and laterbreeding, and realize hybridization through matching of a breeding target and a male parent. The method breaks through the quinoa breeding technology and is fast, accurate and efficient.

The invention relates to a cultivation method of plantlet peppers and belongs to the technical field of biological breeding. The cultivation method comprises the following steps: hybridizing selected new-generation red cluster peppers as male parents with ordinary bullet series red cluster peppers, enabling the ratio of the male parents to female parents to be (1:5)-(1:6), performing emasculation, collecting pollen, performing pollination and harvesting seeds. The by adopting the cultivation method provided by the invention has the advantages that fruits are short and conical, emerald when the fruits are tender, cardinal after the fruits are mature, smooth in fruit surfaces, and heavy; the cultivated peppers have a large number of seeds, disease resistance, drought resistance and high yield; the plant height is 50cm-55cm; compared with an existing bullet series red cluster peppers, pepper plants are relatively short, the picking efficiency can be improved by 10 times, the volume of each plant is small, the plant spacing is 20cm-27cm generally, the cultivation method is suitable for close planting, the yield is high, and the cultivation method is further suitable for planting in open fields, and particularly suitable for planting in half-soil and half-sand neutral lands.

The invention discloses a method for cultivating amomum villosum, including the following steps: Amomum longiligulare T.L.Wu is taken as male parent, and amomum villosum farming cultivation type long fruit No. 2 is taken as female parent; the flower of female parent is protected by bagging in bud pregnant period, and manual emasculation is carried out in blooming period; pollen of male parent is scraped to be pollenized into stigma of the flower of treated female parent, and the flower of female parent is protected by bagging again; after hybridization of female parent, other undeveloped flowers of the inflorescence are removed; when hybridized flower forms a young fruit, a bag protector is removed, and management is reinforced until harvest, thus obtaining hybridized generation F1; seeding, continuous selfing, directional seed selection and cultivation are carried out on the hybridized generation F1 by taking disease resistance, stress resistance, late blooming and high natural maturing rate as seed selection aims, thus obtaining the required amomum villosum. The cultivation method of the invention has simple process and easy control, and amomum villosum new breed having the advantages of disease resistance, stress resistance, late blooming and high maturing rate can be obtained by optimal control on seed selection factors.

The invention relates to a multifunctional self-propelled adjustable boom spraying emasculation machine and belongs to the technical field of agricultural machinery. The multifunctional self-propelled adjustable boom spraying emasculation machine comprises a mechanical traveling and steering system, a cab lifting system, a left-right wheel distance splicing adjusting system, a hydraulic rotary blade emasculation system, a foldable boom spraying system and a hydraulic control system. The multifunctional self-propelled adjustable boom spraying emasculation machine. The multifunctional self-propelled adjustable boom spraying emasculation machine has the advantages that an inner-circulation air conditioner is sealed in a cab, the cab is liftable, and when the cab is lifted, spraying is benefited, good emasculation sight is achieved, and chemical mist is effectively prevented from hurting a driver; the left-right wheel distance of a chemical spraying machine can be adjusted to achieve stable operation and high operation efficiency; the traveling mechanism is driven by a four-wheel chain and adopts flexible hydraulic steering, and power is output synchronously, stably and powerfully; a spraying system is convenient and flexible to operate and simple and practical to manufacture, manufacturing cost is lowered, and boom height can be adjusted at will to adapt to the spraying operation of different fields.

The invention discloses a breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis. The method comprises the following steps: firstly, taking Eucalyptus urophylla U16 serving as a female parent and Eucalyptus grandis G33 serving as a male parent as parents; performing emasculation and bagging, pollen collection, hand pollination, cap bag removal, seed collecting and sowing and breeding to realize consistent character expression and stable inheritance. The clonal DH33-27 variety of Eucalyptus urophylla*E.grandis which is not subjected to segregation of character has the variety characteristics that the leaves are lanceolate, terminal buds are green, veins are obvious, seedling stems are slightly red, tree trunks in the early stage is brownish red, the upper parts of the tree trunks are cyan, and the tree trunks are completely peeled in the later period; the clonal DH33-27 variety of Eucalyptus urophylla*E.grandis has the characteristics of high general performance, straight stem shapes, order forest form, small branches, high annual average amount of growing stock and obvious economic benefit, is free of plant diseases and insect pests and suitable for large-scale cultivation promotion.

The invention discloses a quality detection method for pollen for pollinating Korla fragrant pears. Three-level detection is carried out on the pollen purity, the germination percentage and the fruiting rate of the Korla fragrant pears, and the method comprises the following steps of: physically judging whether the purity of the pollen to be detected is 80 to 100 percent and the impurity particles account for 0 to 15 percent under a microscope according to the principle of metrology; detecting the viability of the pollen population through a germination test, seeding the pollen in a culture medium, keeping moisture at 25 DEG C for four hours, and metering the germination rate of the pollen under the microscope, wherein the average germination rate of qualified pollen is more than or equal to 50 percent; and finally judging the inherent quality of the pollen by combining the emasculation with the artificial pollination technology and considering the inflorescence fruiting rate, the flower fruiting rate and the fruit texture feature of special pollen. The invention creatively combines laboratory analysis and field investigation and analysis, establishes a set of complete and strong-applicability quality detection method for the pollen for pollinating the Korla fragrant pears, and has high application value.

The invention discloses an unmanned emasculation machine. The unmanned emasculation machine comprises an unmanned aerial vehicle, a telescoping mechanism, a mounting rack, two first drive motors, twoflail knife mechanisms, an angle adjusting steering engine, a swing rod and a connecting rod, wherein one end of the telescoping mechanism is detachably connected to the bottom of the unmanned aerialvehicle; the mounting rack is hinged with the other end of the telescoping mechanism; the two first drive motors are arranged at the two ends of the mounting rack respectively; the two flail knife mechanisms are connected with power output ends of the drive motors respectively; the angle adjusting steering engine is fixed at the other end of the telescoping mechanism; one end of the swing rod is connected with a power output shaft of the angle adjusting steering engine; one end of the connecting rod is connected with the other end of the swing rod, and the other end is connected with the mounting rack. The unmanned aerial vehicle is taken as a carrier and drives a cutting device for cutting stamens, a traditional large operation machine is replaced for in-land operation, soil grinding whenthe traditional large operation machine enters the land for operation is avoided, soil hardening is prevented, height and angle are adjusted in a cutting process, and cutting precision is improved.