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Quality detection method for pollen for pollinating Korla fragrant pears

A quality inspection method, the technology of Korla fragrant pear, is applied in the field of pollen inspection and pear pollen quality inspection for pollination. It can solve the problems that the evaluation results cannot meet the requirements of production and the market, and achieve comprehensive inspection items, simple inspection technology, and inspection procedures. full effect

Inactive Publication Date: 2010-09-15
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, for a long time, the detection of pear pollen quality has always been based on the traditional method of simply detecting the pollen germination rate.
This method can only judge the viability of the pollen, but the tree species and variety to which the pollen belongs, the affinity with the main plant species after pollination, and the detection of the texture of the pollen are absent, and the evaluation results made cannot satisfy production and market requirements

Method used

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  • Quality detection method for pollen for pollinating Korla fragrant pears
  • Quality detection method for pollen for pollinating Korla fragrant pears
  • Quality detection method for pollen for pollinating Korla fragrant pears

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Experimental program
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Embodiment 1

[0037]1. Evenly sprinkle the pollen to be tested on a glass slide with a thickness of 0.8-1.0 mm, and observe its morphology under an optical microscope (×100). Select a suitable field of view, adjust the focal length, and judge the shape of a particle in the field of view. Qualified pollen is full in shape, pollen wall is not invaginated, oval or oval, with three obvious germination grooves, the polar axis is 40±5μm, and the red axis is 20±5μm. The purity of qualified pollen should be above 80%, and the number of impurity particles should be less than 15%. The pollen observation field of view of each species for inspection shall not be less than 10. The calculation formula of pollen purity is: pollen purity = number of qualified pollen / total number of particles in the field of view × 100%. The percentage of impurity particles = the total number of non-pollen particles / the total number of particles in the field of view × 100%.

[0038] 2. The formula of the medium used for ...

Embodiment 2

[0041] As is well known in the art, emasculation and bagging techniques are two basic techniques commonly used in artificial cross breeding. One of the purposes of emasculation technology is to prevent natural self-crossing when the two sexes are fully flowered, while the purpose of bagging technology is to prevent natural hybridization caused by wind pollination or insect pollination. The flower organs of pears are complete flowers, and most varieties are heterofloral plants, and the fruit setting rate of self-pollination is extremely low, such as less than 2% for fragrant pears. Traditionally, emasculated flowers are generally combined with bagging to prevent natural hybridization caused by wind pollination or insect pollination. The present invention adopts at the bud stage, i.e. the balloon stage, through artificial emasculation, because the pear flower has not yet opened at this time, and the anthers have not cracked yet, completely eliminating the possibility of natural ...

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Abstract

The invention discloses a quality detection method for pollen for pollinating Korla fragrant pears. Three-level detection is carried out on the pollen purity, the germination percentage and the fruiting rate of the Korla fragrant pears, and the method comprises the following steps of: physically judging whether the purity of the pollen to be detected is 80 to 100 percent and the impurity particles account for 0 to 15 percent under a microscope according to the principle of metrology; detecting the viability of the pollen population through a germination test, seeding the pollen in a culture medium, keeping moisture at 25 DEG C for four hours, and metering the germination rate of the pollen under the microscope, wherein the average germination rate of qualified pollen is more than or equal to 50 percent; and finally judging the inherent quality of the pollen by combining the emasculation with the artificial pollination technology and considering the inflorescence fruiting rate, the flower fruiting rate and the fruit texture feature of special pollen. The invention creatively combines laboratory analysis and field investigation and analysis, establishes a set of complete and strong-applicability quality detection method for the pollen for pollinating the Korla fragrant pears, and has high application value.

Description

field of invention [0001] The invention relates to the technical field of pollen detection. Specifically, the invention relates to the technical field of quality detection of pear pollen for pollination. Background technique [0002] Pear is an important deciduous fruit tree in temperate zone. Most of the cultivated pear varieties are cross-pollinated. When cultivating, proper pollinating trees must be arranged to ensure its yield and quality. Traditionally, the way to solve this problem is to arrange pollinated trees alternately or adjacently in the same orchard, or to graft pollinated varieties on the main species. In recent years, the development of agricultural industrialization has changed the traditional pear planting method. In order to improve the planting efficiency, many commercially cultivated pear orchards are not equipped with pollination trees when they are built. [0003] Pear pollen for pollination is an important means of production. As a special living bi...

Claims

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Application Information

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IPC IPC(8): G01N21/85A01H1/02
Inventor 何天明吴玉霞李疆覃伟铭韩冰
Owner XINJIANG AGRI UNIV
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