Quality detection method for pollen for pollinating Korla fragrant pears
A quality inspection method, the technology of Korla fragrant pear, is applied in the field of pollen inspection and pear pollen quality inspection for pollination. It can solve the problems that the evaluation results cannot meet the requirements of production and the market, and achieve comprehensive inspection items, simple inspection technology, and inspection procedures. full effect
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Embodiment 1
[0037]1. Evenly sprinkle the pollen to be tested on a glass slide with a thickness of 0.8-1.0 mm, and observe its morphology under an optical microscope (×100). Select a suitable field of view, adjust the focal length, and judge the shape of a particle in the field of view. Qualified pollen is full in shape, pollen wall is not invaginated, oval or oval, with three obvious germination grooves, the polar axis is 40±5μm, and the red axis is 20±5μm. The purity of qualified pollen should be above 80%, and the number of impurity particles should be less than 15%. The pollen observation field of view of each species for inspection shall not be less than 10. The calculation formula of pollen purity is: pollen purity = number of qualified pollen / total number of particles in the field of view × 100%. The percentage of impurity particles = the total number of non-pollen particles / the total number of particles in the field of view × 100%.
[0038] 2. The formula of the medium used for ...
Embodiment 2
[0041] As is well known in the art, emasculation and bagging techniques are two basic techniques commonly used in artificial cross breeding. One of the purposes of emasculation technology is to prevent natural self-crossing when the two sexes are fully flowered, while the purpose of bagging technology is to prevent natural hybridization caused by wind pollination or insect pollination. The flower organs of pears are complete flowers, and most varieties are heterofloral plants, and the fruit setting rate of self-pollination is extremely low, such as less than 2% for fragrant pears. Traditionally, emasculated flowers are generally combined with bagging to prevent natural hybridization caused by wind pollination or insect pollination. The present invention adopts at the bud stage, i.e. the balloon stage, through artificial emasculation, because the pear flower has not yet opened at this time, and the anthers have not cracked yet, completely eliminating the possibility of natural ...
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