7 results about "Pollen wall" patented technology

The present application discloses a plant pollen wall development related protein EXA3, a coding gene thereof and an application. The protein provided by the present application is a protein of (a) or (b) as follows: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO. 1; (b) a protein derived from SEQ ID NO. 1, which is substituted and / or deleted and / or added with one or more amino acid residues and related to plant pollen wall development. The plant pollen development related protein of the present application affects the male gametophyte development process of a plant. Inhibition of the expression of the protein coding gene can cause abnormal development of the male gametophyte of the plant and affect the formation of grains, so that a transgenic plant with abnormal pollen wall development and a transgenic plant with male sterility can be cultivated.

A composition comprised of a peroxidase, a catalase, and a peroxide that, in combination, is suitable for degradation of pollen wall sporopollenin, and coatings comprising sporopollenin in a matrix that can be selectively degraded through application of a peroxidase, a catalase, and a peroxide to the sporopollenin matrix.

The present invention discloses a staining solution and a fading / color separation solution for a simple whole-pollen methyl green staining method. The staining solution is composed of methyl green, manganese chloride, manganese sulfate, ethanol, and acetic acid, and the fading / color separation solution is composed of triethanolamine, ethanol, and glycerol. Based on the selective binding of methyl green to DNA and the dual effects of triethanolamine in fading methyl green and transparentizing the pollen wall, the present invention simplifies the post-staining fading, color separation, and transparency processes into a single step. This method not only achieves clear contrast in the staining of pollen nuclei, but also provides an easily controllable staining effect with good repeatability. Furthermore, the method is simple and quick to operate, and has the advantages of safety, environmental protection, and low cost. The method can be used to observe and determine the developmental stage of pollen from cruciferous crops and other crops with similar structures and sizes. It is also expected to find applications in research fields such as plant classification, sterile line conversion, and gene expression during pollen development.

The invention discloses a plant pollen wall development related protein OsEXA5 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1, is related to plant pollen wall development and is derived from SEQ ID NO.1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses plant male sterility related aldehyde oxidase EXA1 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO.1, is related to plant aldehyde oxidase and is derived from SEQ ID NO.1. The plant aldehyde oxidase related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene leads to abnormal development of plant male gametophytes and influences the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterility can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated.

The invention discloses a ternary composite sponge and application thereof, and belongs to the field of biotechnology and enzyme engineering. The ternary composite sponge is prepared from chitosan, pollen wall microgel and diatomaceous earth through ionic crosslinking and freeze drying processes, and the mass ratio of the chitosan to the pollen wall microgel to the diatomaceous earth is (1.25): (0.52): (0.20.6). The carrier has high porosity, large specific surface area and excellent biocompatibility, is suitable for immobilized protease, especially protein glutaminase, shows high enzyme loading capacity, high carrier enzyme activity and good reuse stability, and has wide application prospects in the fields of food processing and enzyme catalysis.

The invention discloses a rape bee pollen crushing and extracting technology, and relates to the technical field of health food, the rape bee pollen crushing and extracting technology comprises the following steps: S1, selecting proper crude rape pollen, and crushing; s2, performing degreasing, drying and wall breaking on the crushed rape pollen; s3, extracting crude flavone in the pollen subjected to wall breaking; when pollen is subjected to wall breaking work by combining an ultrasonic wall breaking method with an enzymolysis method, an enzyme can cut off cellulose or other polysaccharide components of pollen particles, promote protein on the surfaces of the pollen particles and crush the pollen particles into smaller fragments, so that subsequent experimental operation or analysis is facilitated; the pollen is vibrated by using high-frequency sound waves to destroy cell walls, so that chemical substances in cells are more easily extracted; therefore, the pollen wall breaking efficiency is maximized.