47 results about "Pollen wall" patented technology

The invention discloses a method using supercritical carbon dioxide to perform bee pollen broken-face. Bee pollen is put in an extraction plant of supercritical carbon dioxide, to react for a certain time in supercritical carbon dioxide liquid at the proper pressure and temperature, a vent valve is opened to rapidly empty carbon dioxide, so that a pollen cell expands acutely due to larger pressure difference of the inside and the outside, thereby cracking. The method has the advantages that the operation is simple and convenient, the cost is low, the whole process is performed below 50 DEG C, the broken-face time does not exceed 30 minutes, the influence of the product by thermotropy is reduced, so that the active ingredient is preserved completely, at the same time, bacillus can be killed, the unification of the broken-face and the sterilization processes is realized, the broken-face ratio of the pollen cell is high, but the percentage of damage is low, and the inclusion still exists in a cracked pollen wall under the dry condition, thereby favoring the preservation of the nutritious content.

The invention discloses a method for preparing paeonia suffruticosa pollen protein polypeptide with a fermentation process. The method comprises steps as follows: pollen wall breaking, extraction of essential oil, fermentation, degreasing and spray drying. Paeonia suffruticosa pollen is frozen, mechanically crushed and subjected to anaerobic fermentation through lactobacillus plantarum and saccharomyces cerevisiae for wall breaking continuously, then, fermentation is performed through bacillus subtilis, neutral protease produced through fermentation by use of the bacillus subtilis is used for enzymolysis of paeonia suffruticosa protein, the paeonia suffruticosa pollen protein polypeptide with active strength is obtained, and then, chitosan immobilized lipase is used for degreasing a fermenting liquid. The method has the benefits as follows: the prepared paeonia suffruticosa pollen protein polypeptide cannot be hydrolyzed by enteropeptidase, directly acts on an in-vivo receptor and has a remarkable effect on reducing blood pressure. Meanwhile, the paeonia suffruticosa pollen protein polypeptide has an unexpected weight losing effect.

The invention relates to a wet-type wall-breaking method for pollen, which comprises the following steps of: (1), washing, layering and filtering the pollen to remove impurities; (2) soaking the pollen in ethanol and filtering the pollen for standby; (3) adding a mixed solid material to mix the pollen, adding water of which the mass is 1 to 3 times of the total mass of the solid material to obtain an uniform mixed suspension and grinding the suspension to make a paste, wherein the mass ratio of the pollen to the mixed solid material is 1:1.0-5.0; and (4) drying the paste under reduced pressure, and obtaining a product of wall-broken pollen through crushing and screening. The mixed solid material is orally-taken cyclodextrin or a mixture of the orally-taken cyclodextrin and an edible or pharmaceutical solid accessory, wherein the mass ratio of the orally-taken cyclodextrin to the edible or pharmaceutical solid accessory is 1:0.5-4.0. The method combines the pollen wall breaking and the protection of the wall-broken pollen, eliminates the possibility for the oxidization and bacteria infection of the wall-broken pollen and achieves high pollen wall breaking efficiency. The method ensures the stability of the active ingredients of the pollen and the natural flavor of the pollen.

The invention discloses an anther tapetum specific expression promoter POsABCG15. The promoter has a nucleotide sequence shown as SEQ ID NO. 31. The promoter can regulate and control a target gene, so that the target gene is expressed to anther tapetum specifically; and the POsABCG15 in rice regulates and the controls the expression of a lipid deposition gene OsABCG15 on anther epidermis and pollen wall of the rice and influences the accumulation of lipids on surfaces of anther and pollen, so that the anther tapetum specific expression promoter POsABCG15 can be used for generating a new rice male sterile line or regulating and controlling the expression of other genes in the tapetum, and has an important effect in agricultural production.

The invention discloses a pig feed synergist capable of improving productivity. The pig feed synergist is prepared from, by weight, 40-50 parts of cassava leaves, 5-10 parts of shepherd's purse, 20-24 parts of peanuts, 5-10 parts of lemon juice, 20-25 parts of pleurotus eryngii, 10-13 parts of rape pollen, 15-20 parts of pumpkin seed kernels, 8-10 parts of honey, 6-7 parts of green tea, 4-5 parts of licorice roots, 3-4 parts of nanometer montmorillonite powder, a proper amount of Grifola frondosa hyphae, a proper amount of Kombucha and a proper amount of water. By treating cassava leaves with Kombucha, the appetite of bred animals for cassava leaves can be increased, the digestion and absorption of cassava leaves in the bodies of bred animals can be prompted, and the balance of nutritional ingredients in the bodies of bred animals can be improved. As pollen has tough cell walls, multiple enzymes are secreted by Grifola frondosa hyphae and can effectively break pollen walls, nutritional substances in pollen are released, and the content of polysaccharide and protein in fermentation liquor is increased. Nanometer montmorillonite can adsorb germs and heavy metal and effectively solve the problem of antibiotic and heavy metal residuals. The pig feed synergist can increase the appetite, regulate the metabolism of organisms, promote the growth and development of pigs and improve the productivity.

A dry extrusion wall-breaking method for pollen comprises the following steps of: (1) soaking pollen in pure water for 30 minutes and filtering the pollen to remove dust and impurities; (2) adding the pollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / min and a stroke of 80 to 150 centimeters, and injecting out the pollen at a high speed from a die opening with a 2 to 6 millimeter diameter for wall breaking; and (3) drying the pollen for 1 to 2 hours under reduced pressure and filling the pollen in bags for package to obtain the product. The pollen can be pure pollen or a mixture of any several kinds of the pollen. The pollen is selected from hawthorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize pollen, sunflower pollen, astragalus sinicus pollen and the like. The pollen wall-breaking efficiency of the method is up to 99 percent. The sanitation, safety and stability of the pollen product are improved. The method is simple in process, can be operated continuously and is high in yield.

The present invention relates to a pollen wall breaking method. The new pollen wall breaking method comprises the following steps: step 1: dissolving: the granular pollens are ground into powder, and water is added into the powder to blend the powder into pollen solution; step 2: homogenizing: the pollen solution is subjected to at least twice homogenization repeatedly via a high pressure homogenizer at a pressure of 30 MPa-50 Mpa; and step 3: double enzyme digesting: pectinase at a content of 0.5%-2% of the pollens is added into the pollen solution, a pH value is adjusted to be 5, the mixture is put into a water bath at 40-50 DEG C to be stirred and extracted for 5h-7h; cellulose at a content of 0.5%-2% of the pollens is added, the pH value is adjusted to be 5, and the mixture is put into the water bath at 40-50 DEG C to be stirred and extracted for 5h-7h. The homogenizing and double enzyme digesting are used to realize the wall breaking of the pollens and the pollen wall breaking rate is 60% or more.

The invention discloses peony pollen phaseolin polypeptide prepared by using a fermentation method. The phaseolin polypeptide has the weight losing function and excellent blood pressure reduction function. A method for preparing the peony pollen phaseolin polypeptide by using the fermentation method comprises the steps of pollen wall-breaking, essential oil extraction, fermentation, degreasing andspray drying. Peony pollen is frozen and subjected to mechanical pulverization, then lactobacillus plantarum and saccharomyces cerevisiae are adopted for anaerobic fermentation, wall-breaking continues, and then bacillus subtilis is adopted for fermentation; neutral protease generated by fermentation is used for conducting enzymolysis on peony pollen protein, the peony pollen phaseolin polypeptide with active intensity is obtained, and then chitosan immobilized lipase is adopted for degreasing fermentation liquid.

The invention discloses a growth of Chinese cabbage pollen wall related gene PGBc2 and separation thereof. The upgrowth of Chinese cabbage pollen wall related gene includes nucleotide sequence indicated in SEQ ID No. 2. Separation clone and functional verification of the gene is helpful to illustrate molecular mechanism in growth of pollen and male sterility of plants, and has practical meaning for artificially controlling fertility of plant, cultivating superior varieties.

The invention belongs to the field of equipment used for preparing a honey product and more particularly relates to a pollen wall crushing machine which is used for preparing honey scented tea. The pollen wall crushing machine comprises a control cabinet and a motor connected with the control cabinet, wherein an output shaft of the motor is connected with a shocking wall crushing machine; and the shocking wall crushing machine comprises a wall crushing device arranged inside the shocking wall crushing machine. With the adoption of the pollen wall crushing machine disclosed by the technical scheme, wall crushing can be carried out on pollen under a room temperature; the pollen wall crushing machine is simple in structure and convenient to operate; the amount of the pollen subjected to primary wall crushing is not limited; the pollen wall crushing machine is suitable for the use of common middle-size and small-size production enterprises; and the wall crushing efficiency is high, and external environment and temperature are not limited in a wall crushing process.

The invention discloses a method for extracting fatty compounds from bee pollen, which comprises the following steps of: removing water soluble substances outside the pollen wall of the bee pollen; then, extracting the fatty compounds from the bee pollen by using a supercritical carbon dioxide extraction method. The method reduces production cost and the loss of a supercritical carbon dioxide extraction device through pretreatment steps, obviously increases the extraction rate and the yield of the fatty compounds from the bee pollen through supercritical extraction, and also obviously increases the content of fatty acid compounds in the obtained extract.

The invention discloses a rice anther epidermis and pollen wall fat deposition gene OsABCG15 and protein coded by the gene. The protein comprises the amino acid sequence shown as the SEQ ID NO.1 or the amino acid sequence shown as the SEQ ID NO.1 subjected to substitution, deletion or addition of at least one amino acid and has the function as same as that of the rice anther epidermis and pollen wall fat deposition protein. The invention further discloses a recombinant expression vector of the OsABCG15 gene. The protein coded by the OsABCG15 gene has a function of transferring lipid to form specific ornamentation of the anther and the pollen wall. The gene is necessary for microspore to be developed into fertile pollen. Due to deletion of the gene function, the protective layer of the anther and the pollen wall is not formed and rice male sterility is generated.

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.

The invention discloses a tea plant pollen wall breaking method, which relates to the field of tea plant pollen wall breaking. The treated pollen is uniformly moistened by an enzyme solution, is then fast frozen, and is dried by a special drier, granular cells of the pollen are broken, and fovilla is safely released. The invention has the advantages of small investment scale, simple and reliable process, convenient raw materials, low energy consumption, safe operation and stable product quality, and is adaptable to the processing requirements of cleanness, standardization and serialization of the pollen, so the invention has obvious economic and social benefits.

The invention discloses a method for acquiring a transgenic plant by growing related gene PGBc1 using cabbage pollen intine. The method comprises the following steps: constructing an antisense expression vector; constructing a CaMV35S constitutive promoter expression vector and a BcA9 tissue-specific promoter expression vector; genetically transforming the acquired promoter expression vectors on heart of the cabbage; acquiring PGBc1 gene expressing silent and restricted transgenic plants. The method is helpful for clarifying the whole process of pollen wall growth and molecular mechanism of plant male sterility, and has practical significance to manual controlled plant fertility in production and cultivation of excellent varieties.

The invention provides a method for prolonging tobacco pollen storage time by utilizing tobacco seed oil, which comprises the following steps: squeezing out tobacco seed oil by adopting a physical squeezing method; mixing fresh tobacco pollen and dry allochroic silicagel with the particle size of 1-2mm according to the mass ratio of 10:1 in a storage container; and weighing tobacco seed oil according to the proportion that 1ml of tobacco seed oil is added into 1g of pure tobacco pollen, adding the tobacco seed oil into the mixture of the tobacco pollen and the allochroic silicagel, sealing the storage container, repeatedly vibrating for dispersing the air between the tobacco pollen and the allochroic silicagel, and putting at low-temperature environment for storing. Experiments prove that the tobacco seed oil can reduce the breathing rate of the pollen, reduce the deterioration velocity of the pollen and greatly prolong the storage time of the pollen; by adding the allochroic silicagel with a small particle size, the water of the pollen can be lost slowly, and the flexibility of the pollen wall can be enhanced by the tobacco seed oil; the damage to the tobacco pollen caused by desiccation and low temperature is further reduced; and the tobacco seed oil can not influence the biological characters of the tobacco pollen.

The invention relates to the technical field of plant extraction and provides a method for extracting a pollen amino acid liquid from rose pollen. The method comprises steps as follows: step one, the rose pollen is processed, and then boiling water is added for evaporation; step two, a hydrogen dioxide solution is added; step three, water is added, washing is performed, then the mixed solution is left to stand, primary precipitate is obtained through centrifugation, and after the primary precipitate is washed for 3-4 times, final precipitate is obtained; step four, a phosphate buffer solution, cellulase and lysozyme are added to the final precipitate, the mixture is subjected to enzymolysis and then is boiled and centrifuged, and a supernatant is taken as the pollen amino acid liquid. The extraction process does not involve metal grinding, avoids noise and strong corrosive substances and eliminates heavy metal pollution and noise pollution of products; the pollen amino acid liquid contains pollen active substances, is free of chemical agent residues, removes sensitization effect of residual pollen wall and is suitable for being taken as a raw material of health care products and cosmetics; meanwhile, rose liquid waste obtained after essential oil extraction is recycled, so that the rose pollen resource is effectively utilized, and the production cost is greatly reduced.

The invention relates to a method for preparing peony pollen protein polypeptide by using peony pollen as a raw material, the peony pollen protein polypeptide prepared by the method, and the application of the protein polypeptide. The peony pollen protein polypeptide of the present invention is a peony pollen protein, or a peony pollen polypeptide. The dried peony pollen is used as a raw material, and the peony pollen protein is obtained through pollen wall breaking, crude protein extraction, and vacuum drying; the peony pollen protein is subjected to proteolysis, debittering, decolorization, centrifugation or filtration to obtain peony pollen polypeptide. The product can be in the form of powder, granule, tablet, capsule, oral liquid and the like. Peony protein polypeptide has the advantages of lowering cholesterol, lowering blood pressure, promoting fat metabolism, anti-fatigue, enhancing human immunity, regulating human physiological functions, etc. It has broad development and application prospects in the fields of food industry, health care and medicine.

The invention discloses a spleen-strengthening and stomach-nourishing feed for soft-shelled turtles. The spleen-strengthening and stomach-nourishing feed is prepared from the following raw materials in parts by weight: 50-60 parts of rice bran, 20-25 parts of Chinese yam, 4-5 parts of milk powder, 14-19 parts of melon pulp, 6-10 parts of Baijiu, 1-2 parts of adenophora stricta, 60-70 parts of flour weevil larva, 15-20 parts of lentinus edodes, 12-14 parts of cassava leaf, 6-11 parts of camellia powder, 3-4 parts of brown sugar, 2-3 parts of lonicera japonica, 1-2 parts of cassia oil, and a proper amount of flaxseed gum, tea fungus, aspergillus and water. An aspergillus fermentation method is adopted to remove hard pollen walls and to extract nutritional ingredients, so as to promote the digestion and resolving ability of the soft-shelled turtles to a bait, and thereby facilitating the growth.

The invention provides a method for prolonging tobacco pollen storage time by utilizing tobacco seed oil, which comprises the following steps: squeezing out tobacco seed oil by adopting a physical squeezing method; mixing fresh tobacco pollen and dry allochroic silicagel with the particle size of 1-2mm according to the mass ratio of 10:1 in a storage container; and weighing tobacco seed oil according to the proportion that 1ml of tobacco seed oil is added into 1g of pure tobacco pollen, adding the tobacco seed oil into the mixture of the tobacco pollen and the allochroic silicagel, sealing the storage container, repeatedly vibrating for dispersing the air between the tobacco pollen and the allochroic silicagel, and putting at low-temperature environment for storing. Experiments prove that the tobacco seed oil can reduce the breathing rate of the pollen, reduce the deterioration velocity of the pollen and greatly prolong the storage time of the pollen; by adding the allochroic silicagel with a small particle size, the water of the pollen can be lost slowly, and the flexibility of the pollen wall can be enhanced by the tobacco seed oil; the damage to the tobacco pollen caused by desiccation and low temperature is further reduced; and the tobacco seed oil can not influence the biological characters of the tobacco pollen.

The invention relates to a method for planting the monoploid of chrysanthemum. The inventive method finds the external shape mark of chrysanthemum pollen at single corn period, and uses said pollen to induce the callus to obtain the monoploid plant. The invention uses liquid medium to cultivate the pollen and pollen wall, to improve the differentiation ablity of callus. For the chrysanthemum withself-cross and sterility, the inventive pollen cultivation can obtain the monoploid and pure diploid, to be used in cross generation to obtain the better F1 generation quickly.

The invention discloses a black garlic antibacterial soft-shelled turtle feed which is prepared from the following raw materials in parts by weight: 60-70 parts of barley flour, 12-16 parts of lycium barbarum buds, 6-12 parts of red yeast rice, 3-4 parts of spinach seed, 20-25 parts of garlic, 6-7 parts of tenebrio molitor larvae, 15-20 parts of lentinula edodes, 12-14 parts of cassava leaf, 6-11 parts of camellia pollen, 3-4 parts of brown sugar, 2-3 parts of honeysuckle, 1-2 parts of cinnamon oil and appropriate flaxseed gum, kombucha, aspergillus and water. In the invention, hard pollen walls are removed by an aspergillus fermenting process, and nutritional ingredients are extracted, so that the digestion and decomposition abilities of the soft-shelled turtle against bait are enhanced, and a growth promoting effect is realized.

The invention discloses a turtle feed prepared by fermenting with distillers' grains. The turtle feed is prepared from the following raw materials in parts by weight: 30-40 parts of distillers' grains, 20-30 parts of sprouted cargo rice, 10-15 parts of red beans, 30-35 parts of sesame seed leaves, 4-5 parts of Chinese wolfberries, 6-7 parts of tenebrio monitors, 15-20 parts of shiitake mushrooms, 12-14 parts of cassava leaves, 6-11 parts of camellia powder, 3-4 parts of brown sugar, 2-3 parts of honeysuckle flowers, 1-2 parts of cassia oil, an appropriate amount of linseed gum, an appropriate quantity of tea funguses, an appropriate amount of aspergillus and an appropriate amount of water. According to the turtle feed disclosed by the invention, an aspergillus fermentation method is used for removing hard pollen walls and extracting nutrient components, so that the digestion and resolution ability of turtles to baits is improved, and the effect of growth promotion is achieved.

The present invention provides a preparation method of active pollen honey, and relates to the technical field of edible nutrition. The preparation method comprises the following steps: a) collecting edible pollen; b) producing edible culture medium containing at least 5%-35% of sucrose in weight ratio according to the kinds of collected pollen; c) conducting in vitro pollen germination using the edible culture medium to obtain germination liquid; d) subjecting the germination liquid to ultrasonic treatment to obtain active pollen nutrient solution; and e) mixing the obtained active pollen nutrient solution with honey with a weight content of at least 80% to prepare the active pollen honey. The prepared active pollen honey contains various nutritional ingredients and active substances of a large amount of pollen, and the preparation process is simple. The in vitro pollen germination can not only greatly improve pollen wall-broken function, but also avoid destroying various beneficial ingredients of the pollen.

The invention discloses a turtle feed capable of invigorating the stomach and removing toxicity. The turtle feed is prepared from the following raw materials in parts by weight: 30-40 parts of sorghum stalks, 4-5 parts of butter, 10-15 parts of Chinese chestnut kernel powder, 20-24 parts of beetroots, 30-40 parts of sweet potato powder, 6-7 parts of tenebrio monitors, 15-20 parts of shiitake mushrooms, 12-14 parts of cassava leaves, 6-11 parts of camellia powder, 3-4 parts of brown sugar, 2-3 parts of honeysuckle flowers, 1-2 parts of cassia oil, an appropriate amount of linseed gum, an appropriate quantity of tea funguses, an appropriate amount of aspergillus and an appropriate amount of water. According to the turtle feed disclosed by the invention, an aspergillus fermentation method is used for removing hard pollen walls and extracting nutrient components, so that the digestion and resolution ability of turtles to baits is improved, and the effect of growth promotion is achieved.

A dry extrusion wall-breaking method for pollen comprises the following steps of: (1) soaking pollen in pure water for 30 minutes and filtering the pollen to remove dust and impurities; (2) adding the pollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minpollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minand a stroke of 80 to 150 centimeters, and injecting out the pollen at a high speed from a die opening with a 2 to 6 millimeter diameter for wall breaking; and (3) drying the pollen for 1 to 2 hoursunder reduced pressure and filling the pollen in bags for package to obtain the product. The pollen can be pure pollen or a mixture of any several kinds of the pollen. The pollen is selected from hawthorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize polhorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize pollen, sunflower pollen, astragalus sinicus pollen and the like. The pollen wall-breaking efficiency of the method is up to 99 percent. The sanitation, safety and stability of the pollen product are improved. The method is simple in process, can be operated continuously and is high in yield.

The invention relates to equipment in the technical field of pollen production, and discloses a pollen wall breaking device. The pollen wall breaking device comprises a breaker body which is provided with a pollen inlet, wherein a conveying device which is provided with a conveyor belt is arranged in front of the inlet; cold chambers and high-temperature chambers are sequentially arranged at the outside of the conveyor belt; one cold chamber and one high-temperature chamber form a temperature varying unit; the cold chambers and the high-temperature chambers are provided with inlets and outlets, and the conveyor correspondingly pass through the inlets and the outlets; each cold chamber is provided with a refrigerating component through which the temperature of each cold chamber can reach -5 DEG C; each high-temperature chamber is provided with heating parts through which the temperature of each high-temperature chamber can reach 50 DEG C. According to the pollen wall breaking device, one cold chamber and one high-temperature chamber form a temperature varying unit; a plurality of temperature varying units are sequentially arranged at the outside of the conveyor belt; drying chambers are arranged in front of the pollen inlet and the outside of the conveyor at the back of each temperature varying unit; the temperature rapidly varies before wall breaking, so that the pollen wall breaking device has the advantage of improving the pollen wall breaking effect.

The invention discloses a pollen wall-breaking process. The process comprises the following steps: 1, taking a set amount of pollen, and mixing with water; 2, freezing the pollen liquid mixed in the step 1; 3, performing microwave treatment on the frozen pollen pulp obtained in the step 2; 4, adding a compound enzyme into the pollen pulp subjected to microwave treatment in the step 3 for enzymolysis, and then carrying out sufficient enzymolysis according to set time; and 5, concentrating and drying the pollen subjected to sufficient enzymolysis. The invention has the following advantages: (1) the equipment investment cost is low, the popularization and application are easy, and the equipment only needs a refrigerator, a freezer, microwave equipment and a water bath kettle; (2) the wall breaking temperature is low, and the loss of nutritional ingredients is reduced; and (3) due to microwave treatment, a sterilization effect is achieved, and the risk of food damage is reduced in the subsequent processing of pollen food and (4) the processing amount of the pollen can be adjusted as required and (5) the pollen wall-breaking process is relatively simple and easy to operate.

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.