47 results about "Pollen wall" patented technology

The invention discloses a method for preparing paeonia suffruticosa pollen protein polypeptide with a fermentation process. The method comprises steps as follows: pollen wall breaking, extraction of essential oil, fermentation, degreasing and spray drying. Paeonia suffruticosa pollen is frozen, mechanically crushed and subjected to anaerobic fermentation through lactobacillus plantarum and saccharomyces cerevisiae for wall breaking continuously, then, fermentation is performed through bacillus subtilis, neutral protease produced through fermentation by use of the bacillus subtilis is used for enzymolysis of paeonia suffruticosa protein, the paeonia suffruticosa pollen protein polypeptide with active strength is obtained, and then, chitosan immobilized lipase is used for degreasing a fermenting liquid. The method has the benefits as follows: the prepared paeonia suffruticosa pollen protein polypeptide cannot be hydrolyzed by enteropeptidase, directly acts on an in-vivo receptor and has a remarkable effect on reducing blood pressure. Meanwhile, the paeonia suffruticosa pollen protein polypeptide has an unexpected weight losing effect.

The invention discloses peony pollen phaseolin polypeptide prepared by using a fermentation method. The phaseolin polypeptide has the weight losing function and excellent blood pressure reduction function. A method for preparing the peony pollen phaseolin polypeptide by using the fermentation method comprises the steps of pollen wall-breaking, essential oil extraction, fermentation, degreasing andspray drying. Peony pollen is frozen and subjected to mechanical pulverization, then lactobacillus plantarum and saccharomyces cerevisiae are adopted for anaerobic fermentation, wall-breaking continues, and then bacillus subtilis is adopted for fermentation; neutral protease generated by fermentation is used for conducting enzymolysis on peony pollen protein, the peony pollen phaseolin polypeptide with active intensity is obtained, and then chitosan immobilized lipase is adopted for degreasing fermentation liquid.

The invention discloses a growth of Chinese cabbage pollen wall related gene PGBc2 and separation thereof. The upgrowth of Chinese cabbage pollen wall related gene includes nucleotide sequence indicated in SEQ ID No. 2. Separation clone and functional verification of the gene is helpful to illustrate molecular mechanism in growth of pollen and male sterility of plants, and has practical meaning for artificially controlling fertility of plant, cultivating superior varieties.

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.

The invention discloses a tea plant pollen wall breaking method, which relates to the field of tea plant pollen wall breaking. The treated pollen is uniformly moistened by an enzyme solution, is then fast frozen, and is dried by a special drier, granular cells of the pollen are broken, and fovilla is safely released. The invention has the advantages of small investment scale, simple and reliable process, convenient raw materials, low energy consumption, safe operation and stable product quality, and is adaptable to the processing requirements of cleanness, standardization and serialization of the pollen, so the invention has obvious economic and social benefits.

The invention provides a method for prolonging tobacco pollen storage time by utilizing tobacco seed oil, which comprises the following steps: squeezing out tobacco seed oil by adopting a physical squeezing method; mixing fresh tobacco pollen and dry allochroic silicagel with the particle size of 1-2mm according to the mass ratio of 10:1 in a storage container; and weighing tobacco seed oil according to the proportion that 1ml of tobacco seed oil is added into 1g of pure tobacco pollen, adding the tobacco seed oil into the mixture of the tobacco pollen and the allochroic silicagel, sealing the storage container, repeatedly vibrating for dispersing the air between the tobacco pollen and the allochroic silicagel, and putting at low-temperature environment for storing. Experiments prove that the tobacco seed oil can reduce the breathing rate of the pollen, reduce the deterioration velocity of the pollen and greatly prolong the storage time of the pollen; by adding the allochroic silicagel with a small particle size, the water of the pollen can be lost slowly, and the flexibility of the pollen wall can be enhanced by the tobacco seed oil; the damage to the tobacco pollen caused by desiccation and low temperature is further reduced; and the tobacco seed oil can not influence the biological characters of the tobacco pollen.

The invention relates to a method for preparing peony pollen protein polypeptide by using peony pollen as a raw material, the peony pollen protein polypeptide prepared by the method, and the application of the protein polypeptide. The peony pollen protein polypeptide of the present invention is a peony pollen protein, or a peony pollen polypeptide. The dried peony pollen is used as a raw material, and the peony pollen protein is obtained through pollen wall breaking, crude protein extraction, and vacuum drying; the peony pollen protein is subjected to proteolysis, debittering, decolorization, centrifugation or filtration to obtain peony pollen polypeptide. The product can be in the form of powder, granule, tablet, capsule, oral liquid and the like. Peony protein polypeptide has the advantages of lowering cholesterol, lowering blood pressure, promoting fat metabolism, anti-fatigue, enhancing human immunity, regulating human physiological functions, etc. It has broad development and application prospects in the fields of food industry, health care and medicine.

The invention discloses a spleen-strengthening and stomach-nourishing feed for soft-shelled turtles. The spleen-strengthening and stomach-nourishing feed is prepared from the following raw materials in parts by weight: 50-60 parts of rice bran, 20-25 parts of Chinese yam, 4-5 parts of milk powder, 14-19 parts of melon pulp, 6-10 parts of Baijiu, 1-2 parts of adenophora stricta, 60-70 parts of flour weevil larva, 15-20 parts of lentinus edodes, 12-14 parts of cassava leaf, 6-11 parts of camellia powder, 3-4 parts of brown sugar, 2-3 parts of lonicera japonica, 1-2 parts of cassia oil, and a proper amount of flaxseed gum, tea fungus, aspergillus and water. An aspergillus fermentation method is adopted to remove hard pollen walls and to extract nutritional ingredients, so as to promote the digestion and resolving ability of the soft-shelled turtles to a bait, and thereby facilitating the growth.

The invention relates to a method for planting the monoploid of chrysanthemum. The inventive method finds the external shape mark of chrysanthemum pollen at single corn period, and uses said pollen to induce the callus to obtain the monoploid plant. The invention uses liquid medium to cultivate the pollen and pollen wall, to improve the differentiation ablity of callus. For the chrysanthemum withself-cross and sterility, the inventive pollen cultivation can obtain the monoploid and pure diploid, to be used in cross generation to obtain the better F1 generation quickly.

The present invention provides a preparation method of active pollen honey, and relates to the technical field of edible nutrition. The preparation method comprises the following steps: a) collecting edible pollen; b) producing edible culture medium containing at least 5%-35% of sucrose in weight ratio according to the kinds of collected pollen; c) conducting in vitro pollen germination using the edible culture medium to obtain germination liquid; d) subjecting the germination liquid to ultrasonic treatment to obtain active pollen nutrient solution; and e) mixing the obtained active pollen nutrient solution with honey with a weight content of at least 80% to prepare the active pollen honey. The prepared active pollen honey contains various nutritional ingredients and active substances of a large amount of pollen, and the preparation process is simple. The in vitro pollen germination can not only greatly improve pollen wall-broken function, but also avoid destroying various beneficial ingredients of the pollen.

A dry extrusion wall-breaking method for pollen comprises the following steps of: (1) soaking pollen in pure water for 30 minutes and filtering the pollen to remove dust and impurities; (2) adding the pollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minpollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minand a stroke of 80 to 150 centimeters, and injecting out the pollen at a high speed from a die opening with a 2 to 6 millimeter diameter for wall breaking; and (3) drying the pollen for 1 to 2 hoursunder reduced pressure and filling the pollen in bags for package to obtain the product. The pollen can be pure pollen or a mixture of any several kinds of the pollen. The pollen is selected from hawthorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize polhorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize pollen, sunflower pollen, astragalus sinicus pollen and the like. The pollen wall-breaking efficiency of the method is up to 99 percent. The sanitation, safety and stability of the pollen product are improved. The method is simple in process, can be operated continuously and is high in yield.

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.