3 results about "Pollen wall" patented technology

The invention discloses a plant pollen wall development related protein OsEXA5 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1, is related to plant pollen wall development and is derived from SEQ ID NO.1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses plant male sterility related aldehyde oxidase EXA1 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO.1, is related to plant aldehyde oxidase and is derived from SEQ ID NO.1. The plant aldehyde oxidase related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene leads to abnormal development of plant male gametophytes and influences the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterility can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated.

The invention discloses a ternary composite sponge and application thereof, and belongs to the field of biotechnology and enzyme engineering. The ternary composite sponge is prepared from chitosan, pollen wall microgel and diatomaceous earth through ionic crosslinking and freeze drying processes, and the mass ratio of the chitosan to the pollen wall microgel to the diatomaceous earth is (1.25): (0.52): (0.20.6). The carrier has high porosity, large specific surface area and excellent biocompatibility, is suitable for immobilized protease, especially protein glutaminase, shows high enzyme loading capacity, high carrier enzyme activity and good reuse stability, and has wide application prospects in the fields of food processing and enzyme catalysis.