Method for Prolonging Storage Time of Tobacco Pollen by Using Tobacco Seed Oil

A technology for tobacco seeds and storage time, applied in the directions of botanical equipment and methods, application, and plant preservation, can solve the problems of easily damaged tobacco pollen, high energy consumption, low refrigeration temperature, etc. The effect of deterioration speed and high seed yield

Active Publication Date: 2017-06-20
YUXI ZHONGYAN TOBACCO SEED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to solve the problems in the prior art that when tobacco pollen is frozen and stored, the refrigeration temperature required is relatively low and energy consumption is large; and when it is stored at low temperature, the operation is complicated when thawing, and the tobacco pollen is easily damaged, etc., to provide a method for using The method for prolonging the storage time of tobacco pollen by tobacco seed oil solves the problems existing in the prior art

Method used

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  • Method for Prolonging Storage Time of Tobacco Pollen by Using Tobacco Seed Oil
  • Method for Prolonging Storage Time of Tobacco Pollen by Using Tobacco Seed Oil
  • Method for Prolonging Storage Time of Tobacco Pollen by Using Tobacco Seed Oil

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Pick the flowers of Yunyan 87 from the bud-bearing stage to the flowering stage, collect the anthers, dry them to loosen the powder, and sieve the pollen and anther sacs with a 50-mesh sieve. Weigh 10 g of Yunyan 87 fresh pollen and 1 g of dry color-changing silica gel with a particle size of 1-2 mm, mix them in a 25 ml centrifuge tube, add tobacco seed oil into the centrifuge tube containing the mixture of tobacco pollen and color-changing silica gel, Close the cap of the centrifuge tube, shake it repeatedly, drive out the air between the tobacco pollen and the color-changing silica gel, and store it in an air-conditioned room at 17±1°C. The experiment is divided into three treatment methods and a control group, each of which is carried out three times:

[0020] Treatment 1: Measure 5 ml of tobacco seed oil and add it to a centrifuge tube containing a mixture of tobacco pollen and color-changing silica gel.

[0021] Treatment 2: Measure 10 ml of tobacco seed oil into a...

Embodiment 2

[0039] Repeat the experiments of the control group and treatment 2 in Example 1, the difference is: store in the refrigerator at 4±1°C, before storage and storage for 3 months, 6 months, 9 months, and 12 months The pollen viability was then measured. The test results are shown in Table 2.

[0040] The results showed that the pollen viability of Yunyan 87 declined rapidly during the control storage period, and the pollen viability of the 6-month storage was significantly lower than that of the 3-month storage. During the storage period of treatment 2, the pollen viability decreased slowly, and the pollen viability in each time period was significantly better than that of the control; the pollen viability of the 9-month storage was not significantly different from that of the 6-month storage, and the pollen viability of the 12-month storage was significantly reduced. It shows that tobacco pollen can be stored for 9 months by this method.

[0041]Table 2 Changes in pollen viab...

Embodiment 3

[0044] Repeat the pair group and treatment 2 experiments in Example 1, the difference is: store in a refrigerator at -20±1°C, measure pollen vigor before storage and after 1 year, 2 years, and 3 years of storage; Before storing in the refrigerator at -20±1℃, pre-cool in the refrigerator at 4℃ for 24 hours. The test results are shown in Table 3.

[0045] The results showed that the pollen viability of Yunyan 87 declined rapidly during the control storage period, and the pollen viability of the one-year storage was significantly lower than that before storage. During the storage period of treatment 2, the pollen viability decreased slowly, and the pollen vigor of each time period was significantly better than that of the control; the difference between the pollen viability of the 2-year storage and the 1-year storage was not significant, and the pollen viability of the 3-year storage was significantly reduced . It shows that tobacco pollen can be stored for 2 years by this met...

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Abstract

The invention provides a method for prolonging tobacco pollen storage time by utilizing tobacco seed oil, which comprises the following steps: squeezing out tobacco seed oil by adopting a physical squeezing method; mixing fresh tobacco pollen and dry allochroic silicagel with the particle size of 1-2mm according to the mass ratio of 10:1 in a storage container; and weighing tobacco seed oil according to the proportion that 1ml of tobacco seed oil is added into 1g of pure tobacco pollen, adding the tobacco seed oil into the mixture of the tobacco pollen and the allochroic silicagel, sealing the storage container, repeatedly vibrating for dispersing the air between the tobacco pollen and the allochroic silicagel, and putting at low-temperature environment for storing. Experiments prove that the tobacco seed oil can reduce the breathing rate of the pollen, reduce the deterioration velocity of the pollen and greatly prolong the storage time of the pollen; by adding the allochroic silicagel with a small particle size, the water of the pollen can be lost slowly, and the flexibility of the pollen wall can be enhanced by the tobacco seed oil; the damage to the tobacco pollen caused by desiccation and low temperature is further reduced; and the tobacco seed oil can not influence the biological characters of the tobacco pollen.

Description

technical field [0001] The invention belongs to the technical field of plant pollen storage, and relates to a method for prolonging the storage time of tobacco pollen by using tobacco seed oil. Background technique [0002] In the work of crop male sterile lines and hybrid breeding, it is necessary to collect and store pollen to maintain pollen vitality. Pollen vigor directly affects the success rate of breeding seeds and the quantity and quality of offspring seeds. [0003] In the work of tobacco seed breeding, the pollen collection period of the male parents is 30-40 days, while the pollination period of each batch of female parents is only 15-20 days, and the growth periods of the parents are generally different. Therefore, in order to meet the Large-scale and multi-batch cross-pollination requires intensive collection of pollen from the male parent in advance and storage before it can be used for artificial cross-pollination of the female parent. At room temperature an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
Inventor 马文广郑昀晔禹晓梅牛永志杨晓东索文龙薛大章
Owner YUXI ZHONGYAN TOBACCO SEED
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