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Method for extracting protoplast from pear flower powder by citric acid

A technology of protoplasts and citric acid, applied in the fields of biochemistry and food science, can solve the problems of pollen protoplasts losing biological vitality, inability to obtain pollen protoplasts, and inability to be suitable for plant pollen, etc., achieving easy operation and practical application, and simple structure , the effect of developmental synchronization

Inactive Publication Date: 2008-02-13
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although there are these methods for removing the wall, there are many problems in these methods, and the main problems have: one is that the pollen protoplasts obtained by these methods not only lose their original biological vitality, but also bring certain nutrients to the nutrients. The destructiveness that reduces to a great extent the nutritive value that has; The 2nd is that these methods that obtain pollen protoplast cannot be suitable for all plant pollen, such as, can't obtain the pollen of Rosaceae fruit tree such as pear, apple with these methods Protoplast

Method used

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  • Method for extracting protoplast from pear flower powder by citric acid

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Embodiment Construction

[0023] Extracting plant pollen (tube) protoplasts is one of the necessary premises for studying the physiological and biochemical characteristics and functions of pollen (tube). We applied the method of the present invention to obtain vigorous pear pollen tube protoplasts, and used patch clamp to directly identify the calcium channel of the pollen tube, the calcium gradient in the pollen tube and its related functions, in order to broaden and in-depth study the electrical power of the pollen tube. The chemical properties lay the foundation. It is well known that obtaining pollen (tube) protoplasts is a prerequisite for conducting patch-clamp experimental research. So far, lasers have been used to remove cell walls in root hairs, pseudoroots and hyphae of algae. This method requires more expensive experimental equipment. At present, the active and complete protoplasts obtained from pollen are all made of young and fresh pollen. However, there is no report on pollen that can only be...

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Abstract

The invention relates to a method for extracting protoplast from pear pollen and pollen tube, relating to method of breaking plant cell wall and getting protoplast, belonging to biochemistry and foodstuff field; which mainly applying the property of pollen cell wall to get intact pollen or pollen tube protoplast with original activeness. The invention employs method of removing pollen wall with citric acid, which is to add dry pollen into the citric acid solution of 2-3mol / l, culturing for about 30 minutes, the pollen protoplast getting out of the sprout, centrifuging and getting pollen protoplast with wall removed, the protoplast got from this method is characterized by the retaining nutriment, no pollution, simple process, low cost and suited to be applied in foodstuff field.

Description

Technical field [0001] The invention is a method for extracting pear pollen and pollen tube protoplasts, relates to a method for breaking plant cell walls and obtaining viable protoplasts, and belongs to the fields of biochemistry and food science. Background technique [0002] Plant pollen (pollen) not only plays an extremely important role in plant proliferation, expansion of genetic composition and distribution range, but also extremely rich in nutrients. Recent research shows that pollen contains a wide variety of carbohydrates, organic acids, proteins, nucleic acids, enzymes and coenzymes, pigments, vitamins and minerals and other nutrients. Among them, the protein content is very rich, reaching 7%-40%, which is better than casein. Pollen contains 18 kinds of amino acids, including 8 kinds of amino acids that are necessary for the human body, and its content is 5-7 times more than milk and eggs. In particular, the content of lysine is particularly rich, which is beneficial t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/076A23L21/20
Inventor 张绍铃屈海泳吴俊
Owner NANJING AGRICULTURAL UNIVERSITY
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