Cultivation method for Isolated microspore of cucumber

A cultivation method and microspore technology, applied in the field of biotechnology science, can solve problems such as heavy workload, difficult to use, and long cycle, and achieve the effects of improving resistance and quality, improving breeding efficiency, and shortening the breeding period

Inactive Publication Date: 2008-12-10
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the first three methods need to plant larger plant populations for artificial induction, which has a huge workload, long cycle, time-consuming and labor-intensive, high cost and low induction rate, and is difficult to use; while un

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cultivation method for Isolated microspore of cucumber
  • Cultivation method for Isolated microspore of cucumber
  • Cultivation method for Isolated microspore of cucumber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Haploid and double haploid have important significance and broad application prospects in theoretical research and breeding practice of crop genetics and breeding. The ways to obtain haploid materials include naturally occurring haploids and artificially induced haploids. The frequency of the former is extremely low; the latter is most widely used in free microspore culture. Free microspore culture is a method in which microspores freed from anthers are used as explants for in vitro culture. Microspores have the advantages of haploidy, simple structure, rich genotypes, synchronous development, and a large population. Therefore, in vitro culture of microspores can better control the conditions of androgenesis and quickly obtain haploids of various genotypes. Somatic or double haploid materials are also more prone to gene mutations to obtain new germplasm and expand the cucumber gene pool. We have obtained double haploid cucumber material by applying the method of the p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for cultivating isolated microspore of cucumber, belonging to the field of biological technology. The method of the invention comprises the steps as follows: taking buds, identifying the development period of the microspore, pre-disposing, disinfecting the buds, isolating the microspore with an extrusion method by using B<5>-13 extracting solution, filtrating, centrifuging and purifying the microspore by cell screens with the size of 76mum, carrying out static dark cultivation at the temperature of 25 DEG C till the embryoid can be seen by naked eyes, forming cotyledonary embryoid by surging dark cultivation under the frequency of 60r/min<-1> subsequently, forming regenerated plants or rootless seedlings on MS-BA embryoid germination culture mediums, and finally gaining entire regenerated plants by inducing the roots on the rooting culture medium MS-IBA. The method of the invention can gain haploid or double haploid. The double haploid is good seedling raising material and can be used for the selection of new type or improvement of type, thus greatly shortening the seedling raising period and improving the selection efficiency; the double haploid is also ideal material for carrying out the molecular marking such as AFLP, RFLP, RAPD, and the like, the drawing and researching of genetic maps, the species evolution research, genetic analysis, plant gene cloning selection, etc.

Description

technical field [0001] The invention relates to a method for cultivating cucumber free microspores, which relates to the isolation, purification and suspension preparation of cucumber microspores and a method for cultivating free microspores, and belongs to the field of biotechnology science. Background technique [0002] Haploid refers to an individual with only the number of gamete chromosomes, which is of great significance in crop breeding practice and theoretical research on genetic breeding. Homozygous double haploids can be obtained by chromosome doubling of haploids. In breeding practice, pure lines, that is, double haploid populations, can be obtained by doubling the chromosomes of haploids, but it takes 6 to 7 years or even longer to cultivate inbred lines by conventional breeding methods. At the same time, double haploids are absolutely homozygous, and the genotype and phenotype are completely consistent, while the inbred lines obtained through advanced selfing s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01H1/08A01H1/04C12N3/00C12N5/04
Inventor 陈劲枫詹艳钱春桃娄群峰
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap