19 results about "Ovule" patented technology

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.

Methods and compositions used to prevent or decrease reduction, or reduction and recombination, during meiosis in female sporocyte, female gametophytes, or female gametes are described herein. The compositions and methods may include silencing elements and / or use gene-editing technology to decrease or inhibit Nrf4 expression levels or activity. Further provided are compositions and methods for the production of a plant that has ovules comprising non-reduced, or non-reduced and non-recombined, gametes, and / or seeds that contain clonal maternal embryos.

The invention discloses an oat glume-cutting hybridization method capable of increasing the maturing rate and application thereof.According to the oat glume-cutting hybridization method, by conducting castration and glume-cutting treatment on a first main grain of an outermost spikelet of each round layer in a female parent, it can be guaranteed that ovules obtained after artificial hybridization pollination have sufficient nutrition for grain filling according to the oat fruiting nutrition distribution rule; female parents are bagged after castration and glume cutting, the bagging time is controlled to be 12-24 h, the water content of the fluff surface of the stigma can be reduced (it is prevented that pollen is separated by a water film when falling on the stigma), the contact area of the pollen and the stigma is increased, and the germination rate of pollen tubes is increased; pollination is carried out when the male parent anther naturally cracks, that is, pollination is carried out in the period with the maximum pollen viability, and the cross pollination probability can be increased. According to the method, the hybridization maturing rate can be remarkably increased, and the hybridization maturing rate can reach 70% or above when the white swallow No. 2 is used as the female parent and the white swallow No. 7 is used as the male parent for hybridization.

The present application relates to the field of callus induction, and particularly relates to a kind of induction medium and its application in the induction of white peony root leaf begonia unpollinated ovule callus, the induction medium includes basic medium, plant growth regulator, carbon source and coagulant;The basic medium is any one of B5 medium, Miller medium;The plant growth regulator includes TDZ, NAA and 6-BA;The concentration ratio of TDZ, NAA and 6-BA is TDZ:NAA:6-BA=(0.05-0.10):(0.25-1.00):(0.25-1.00);The pH value of the induction medium is 5.8-6.0.The present application establishes efficient white peony root leaf begonia callus induction system by adjusting the components of culture medium, and obtains embryoid and sprout by inducing callus redifferentiation, which lays a foundation for carrying out begonia haploid breeding research.

The invention provides a method for in vitro pollination and fertilization of amaryllis ovules. The process steps include: sterilizing the transparent flower buds of the male parent of amaryllis, taking the unpolluted anthers and drying them in a sterile beaker to scatter the pollen. Collecting sterile pollen and treating at 4°C for 3 days. After sterilizing, the ovary of the female parent of amaryllis that opened on the same day is separated from the carpel and the ovules are fully exposed, inoculated into a solid and liquid double-layer culture medium, and a large amount of sterile pollen is spread, the medium is sealed with an airtight sealing film, and the medium is cultured in complete darkness for 3 days. The ovules are separated and transferred to a solid MS culture medium, the medium is sealed with a semi-permeable membrane, and the medium is cultured in complete darkness for 45 to 60 days. The fertilized ovules are selected and moved to the medium for culture under light for 30 days. The hybrid zygotic embryos germinate, and finally the hybrid offspring is obtained. The invention obtains the hybrid of amaryllis for the first time through the in vitro fertilization technology, the method is simple and easy to operate, and can effectively overcome the pre-fertilization obstacles of amaryllis distant hybridization and self-pollination with some varieties, and provides technical support for the hybrid breeding of amaryllis.

The invention discloses a method for determining pollination affinity among camellia oleifera varieties based on pollen tube in-situ germination, and belongs to the technical field of agricultural planting. The method comprises the following steps: collecting pistil samples which are pollinated for 48-72 hours, fixing and slicing, and quantifying the pollination affinity by adopting fluorescence microscopic observation in combination with a five-level weighted scoring system (1 score of stigma, 2 scores of upper part of stylus, 3 scores of lower part of stylus, 4 scores of ovary cavity and 5 scores of ovule). Compared with a traditional fruit setting rate method, the method has the advantages that the detection period is shortened from 1-3 months to 3 days, the affinity of different pollination variety combinations can be distinguished more accurately, and the method is particularly suitable for variety matching selection in camellia oleifera cross breeding.

The invention discloses application of a pepper miR167b gene in regulation and control of plant growth and development, and belongs to the technical field of gene engineering. An expression vector of the miR167b gene is constructed and is overexpressed in tomatoes, and the results show that the miR167b overexpressed plant is dwarfed, the fruit shape is changed, the fruit size and weight are obviously lower than those of a wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit ventricles is reduced, ovules are aborted, and no embryo sac is formed in the tomato. Further analysis finds that the content of indolebutyric acid in overexpressed plant fruits is increased, which indicates that the miR167b gene can induce apomixis of fruits by regulating auxin, lays a foundation for revealing a pepper apomixis mechanism, provides gene resources and basis for improving a woody plant breeding strategy, and has important theoretical value and application prospect.

The invention discloses a rapid seedless grape breeding method in the technical field of plant cultivation, which comprises the following steps: step 1, selecting male and female parents and hybridizing: selecting seedless excellent varieties as female parents and male parents respectively, performing artificial inflorescence emasculation on the female parents by using tweezers, bagging and marking, and performing artificial pollination; step 2, embryo rescue: shearing and taking back the hybridized clusters, washing the fruits with running water for 2-4 hours, transferring the fruits into a bottle for disinfection, stripping ovules, inoculating the taken ovules into an embryo development culture medium for dark culture, and after 8-10 weeks, cutting off the embryos and inoculating the embryos into an embryo germination culture medium for light culture; and step 3, transplanting and seedling hardening. The method is simple and easy to operate, through the design of the functional scissors, a sample is disinfected and coated with a culture solution while shearing is achieved, then the sample is disinfected, the activity of the sample is kept, and a good foundation is laid for subsequent seedless grape cultivation.

The invention discloses a promoter for regulating and controlling specific expression of a target gene in tobacco flower tissue and application of the promoter. The sequence of the promoter is SEQ ID No.1. The promoter provided by the invention can regulate and control the specific expression of a target gene in tobacco anther, avoids the expression of the gene in ovaries, ovules and seeds, has important practical value in the aspect of tobacco transgenic breeding, and also can provide reference for the creation of breeding materials of other crops.

The invention relates to the technical field of plant sexual reproduction, in particular to a plant embryo in-vitro rescue method which comprises the following steps: selecting a plant female parent needing embryo rescue; preparing a treating fluid containing a plant hormone regulator; fixing a continuous release device on a branch below the silique or legume of the maternal plant; the treatment liquid is applied to the continuous release device, so that active ingredients in the treatment liquid are absorbed by the branches and transported to the siliques or pods. The externally applied plant hormone regulator can be upwards transported to ovules through the phloem and the xylem and directly acts on the hybrid embryo with development stagnation. Hormonal balance in the embryo is remodeled through cooperative regulation and control of hormones, and a stagnation signal is reversed into a development signal, so that the normal development process of the embryo is rescued and completed in situ without in-vitro operation, and the method is simple to operate, high in success rate, time-saving and labor-saving, and convenient to popularize and apply.

To provide a pretreatment method for observing or extracting embryos in tomato ovules, which enable easy extraction and observation of embryos in tomato ovules.SOLUTION: A pretreatment method for observing or extracting embryos in tomato ovules involves immersing tomato ovules in an aqueous solution of sodium hypochlorite in an amount of 0.2 mass% or more and 0.6 mass% or less.SELECTED DRAWING: Figure 5

The invention relates to an OsEPF1 gene for regulating and controlling generation of rice macrospores, in particular to participation of a plant secretory peptide OsEPF1 rich in cysteine in regulating and controlling the generation process of the rice macrospores. Compared with wild type ZH11, the OsEPF1 gene function deletion mutant for regulating and controlling rice macrospore generation has obvious defects in female germ cell development, especially in the early ovule development stage and the AC and MMC stages, the mutant can generate a plurality of AC / MMC-like cells, then fertility is affected, and the fruiting rate of a rice single plant is reduced; the pEPF1:: EPF1 is supplemented back into the mutant postovule, the growth process of the macrospore is normal, and the maturing rate can be recovered to be normal. The invention proves that the OsEPF1 affects the development of female gametophytes and finally affects the maturing rate of rice by regulating the transformation from somatic cells to germ cell fate in the rice macrospore generation process, and the discovery has important significance on the research on the determination of the rice female germ cell fate, and has certain application value on rice breeding work.

The application discloses a method for separating and culturing ovary granulosa cells of Chinese soft-shelled turtles, which comprises the steps of selecting female Chinese soft-shelled turtles in the positive oviposition period, executing cervical dislocation after anesthesia, killing the turtles, separating ovary tissues, removing redundant parts after washing, separating II-III stage follicles in a basic culture medium, keeping follicle integrity, performing enzyme digestion treatment, removing supernatant through centrifugation, obtaining a precipitate containing ovules with a diameter of 0.1-0.5 cm, resuspending the precipitate by using a mixed culture medium, and inoculating and culturing. The ovary granulosa cells obtained by the separation method have a high yield, the ovary granulosa cells are cultured in the specific mixed culture medium, the method can replace many factors, the amount of added factors is small, the NaHCO3-free culture medium is used, the use of CO2 is reduced, and the ovary granulosa cells obtained are consistent with the in-vivo environment, so that the cost is reduced and the in-vitro survival period of the ovary granulosa cells is prolonged.

Rising global temperatures negatively impact crop yields by inhibiting seed and fruit production, primarily due to the vulnerability of pollen tube (PT) delivery of sperm cells to ovules under heat stress (HS). Two in vivo assays were developed to investigate how various HS conditions affect pollen-pistil interactions in Arabidopsis, revealing that HS impairs multiple stages of PT growth: pollen germination, growth in the transmitting tract, emergence from the transmitting tract, and ovule targeting, and seed formation. Increased temperatures progressively halted PT growth earlier in its journey toward the ovules. This disruption of pollen-pistil interactions may contribute to reduced crop yields under HS. Additionally described are expression cassettes designed to generate heat-tolerant plants by overexpressing LLG, offering a potential solution to mitigate the adverse effects of heat stress on crop production.

The present invention relates to a method for calculating the single-fruit weight of oil-tea camellia based on probability statistics. First, three fruit traits of the oil-tea camellia fruit, namely the number of carpels, the number of fertile locules, and the single-fruit weight, are observed, and the average single-fruit weight of fruits with different numbers of carpels and different numbers of fertile locules is statistically analyzed. Then, the number of carpels, the number of ovules, and the ovule abortion rate of the fruit are set, and the probability of fruits with different numbers of fertile locules is calculated using the binomial formula. Finally, the single-fruit weight of the oil-tea camellia under the set conditions is calculated based on the average single-fruit weight of fruits with different numbers of fertile locules and their probabilities. The present invention can calculate the single-fruit weight of the oil-tea camellia according to the target of variety design, and can be applied to the parental selection in molecular design breeding and cross-breeding of the oil-tea camellia, and the early selection of hybrid and backcross offspring.

A Chinese softshell turtle ovarian granular cell separation and culture method comprises separation and culture, the separation comprises the following steps: selecting a female Chinese softshell turtle individual in a positive spawning period, anesthetizing the female Chinese softshell turtle individual, killing the female Chinese softshell turtle individual after neck breaking, separating ovarian tissues, cleaning, removing redundant parts, placing the female Chinese softshell turtle individual in a basal culture medium, separating follicles in II-III periods, keeping the integrity of the follicles, performing enzyme digestion treatment, and culturing the follicles in the basal culture medium. And centrifuging to remove the supernatant to obtain a precipitate containing egg granules with the diameter of 0.1-0.5 cm, resuspending the precipitate by adopting a mixed culture medium, and inoculating and culturing. The ovarian granulosa cells separated by the separation method provided by the invention are high in yield, the method for culturing the ovarian granulosa cells in a specific mixed culture medium is diversified in alternative factors and small in added factor amount, meanwhile, a NaHCO3-free culture medium is used, the use of CO2 is reduced, the obtained ovarian granulosa cells are kept consistent with the internal environment of an organism, the cost is reduced, and the method is suitable for large-scale popularization and application. And the in-vitro lifetime of the ovarian granular cells is prolonged.

The invention discloses a method for creating a novel chrysanthemum germplasm with high linarin content based on distant hybridization between opisthopapus and chrysanthemum, which comprises the following steps: inducing ovules after pollination for 13-18 days to generate calluses and obtain distant filial generation plants by combining artificial cross breeding with an immature embryo rescue technology, and carrying out morphological and cytological identification to obtain the novel chrysanthemum germplasm with high linarin content. And obtaining a true hybrid filial generation. The content of linarin in inflorescences and leaves of true hybrid filial generations is obviously increased and is obviously higher than that of the female parent Jinlingzirobe. The invention provides a new way for efficient utilization of opisthopapus and chrysanthemum germplasm resources, and has significant breeding and application values.

The invention discloses a fluorescence microscopic detection method for rapidly detecting the life of ovules of fructus amomi, which specifically comprises the following steps: (1) sampling: bagging to-be-detected flowers of fructus amomi before flowering, and taking small flowers of different flower ages at intervals from fresh ovary to withered ovary after flowering; (2) ovule treatment: stripping ovule-containing fructus amomi flower ovules of different flower ages, and sequentially carrying out FAA fixation, NaClO solution transparentizing, NaOH solution softening and aniline blue solution dyeing treatment; (3) loading and observing: longitudinally cutting the treated ovary, tightly attaching the cut surface filled with ovules to a glass slide, covering another glass slide, pressing, and observing whether the vascular bundle of the ovules on the cut surface of the ovary develops color or not under a fluorescence microscope; and (4) determining the service life of the ovules: in the detected ovules at interval flower ages, taking the flower ages before bright green fluorescence display of the vascular bundles of the ovules as the service life of the ovules. The method can quickly detect the life of the ovules of the fructus amomi, and lays a foundation for research on insemination and reproduction of flowers of the fructus amomi and increase of the fruiting rate in the future.

The invention relates to a receptor-like kinase OsERL gene for regulating and controlling development of rice female gametophytes and participation of the receptor-like kinase OsERL gene in regulation and control of development of rice female gametophytes. Compared with a wild type ZH11, the OsERL gene function deletion mutant for regulating and controlling the development of the rice female gametophyte has the advantages that the development of female germ cells has obvious defects, so that the fruiting rate of a single rice plant is reduced; the pERL:: ERL is supplemented back to the mutant postovule, the female gametophyte develops normally, and the maturing rate can be recovered to be normal. The research proves that the OsERL influences the maturing rate of the rice by regulating and controlling the development of the rice female gametophyte, and the discovery has important significance on the research on the fate decision of the rice female germ cells and has certain application value on the rice breeding work.