23 results about "Embryo culture" patented technology

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

This invention provides an independent embryo culture dish, comprising a dish body, several culture chambers, and several waste liquid tanks. The culture chambers are arranged in a matrix on the dish body and connected to the dish body. A waste liquid tank is provided on one side of each row of culture chambers. The interior of each culture chamber is divided into an embryo culture layer, a covering oil layer, and an overflow control layer from bottom to top. A through-channel is provided between the overflow control layer and the waste liquid tank, and the overflow control layer and the waste liquid tank are connected by the through-channel. By setting an embryo culture layer, a covering oil layer, and an overflow control layer arranged from bottom to top in each independent culture chamber, this invention not only effectively ensures a stable environment for the embryo culture medium, but also achieves automatic discharge of excess culture oil through the through-channel structure between the overflow control layer and the waste liquid tank, avoiding culture environment disorder and cross-contamination caused by excessive oil layer thickness.

This invention relates to a medaka hatching enzyme, its preparation method, and its application. The preparation method of the medaka hatching enzyme includes the following steps: 1) collecting fertilized medaka eggs; 2) incubating the fertilized medaka eggs with embryo culture medium in a 26℃ incubator for 6 days, and collecting mature embryos; 3) adding NaCl solution and Tris-HCl buffer (pH=8.0) to the mature embryos, grinding them, collecting the filtrate, and allowing it to stand overnight at 4℃; 4) centrifuging the filtrate at 15000 rpm for 15 minutes, and extracting the supernatant to obtain the medaka hatching enzyme solution. The preparation method is simple to operate, and the obtained medaka hatching enzyme has high activity, which can help to quickly remove the medaka eggshell artificially. The naked medaka embryos obtained by this method can be directly used for in vivo imaging and in-situ quantitative analysis, and embryo model construction, providing support for subsequent genetic, developmental biological, and toxicological studies of medaka embryos.

This invention provides an application and method of lysophosphatidylcholine (LPC) in promoting early development of reconstructed embryos, belonging to the field of embryo engineering technology. The invention first prepares reconstructed embryos, then adds lysophosphatidylcholine to the culture system for culturing. Lysophosphatidylcholine effectively promotes early development of the reconstructed embryos; at an optimal concentration of 5 μM, the blastocyst development rate increases to 24.56%, providing a novel and operable biochemical intervention target to solve the problem of low reconstructed embryo culture efficiency. This invention not only fills the gap in our understanding of the mechanism of action of LPC in this field, but also achieves controllability and reproducibility of the technical solution through precise definition of the optimal concentration. It provides key parameters for establishing standardized culture procedures and provides a more solid foundation for downstream applications such as developmental biology research and disease model construction that rely on high-quality reconstructed embryos, significantly accelerating the process of related scientific discovery and application development.

This application provides the use of purine nucleoside phosphorylase inhibitors in improving the reproductive function of subjects. This application provides the use of purine nucleoside phosphorylase inhibitors in the preparation of drugs for antagonizing hydrosalpinx toxicity or improving the reproductive function of subjects. This application also provides the use of purine nucleoside phosphorylase inhibitors in the preparation of reagents for embryo culture and gamete treatment. This application utilizes purine nucleoside phosphorylase inhibitors to inhibit PNP activity, antagonizing hydrosalpinx toxicity caused by purine metabolism disorders, thereby improving the reproductive function of subjects.

This invention discloses a method for determining glucose content in embryo culture medium. In this invention, proteins and amino acids are filtered from the sample using ultrafiltration centrifuge tubes. High-performance liquid chromatography (HPLC) is used as the detector, and an external standard curve method is employed to detect the glucose content in the embryo culture medium. The pretreatment step of ultrafiltration centrifuge tube filtration eliminates interference from human serum albumin and some amino acids in the embryo culture medium on the determination of the target component, glucose. An amino-based column is specifically selected; the hydroxyl groups in glucose form hydrogen bonds with the amino groups on the stationary phase of the amino-based column, separating them from other components in the culture medium under the action of the mobile phase, thus achieving separation. The method uses a differential detector to establish an external standard curve for quantitative determination, which is characterized by rapid and simple operation, good repeatability, and accurate results, making it suitable for determining glucose content in embryo culture medium.

The utility model relates to the field of assisted reproductive technology equipment discloses a kind of bovine in vitro embryo culture environment simulation device, including device body assembly, the device body assembly includes simulation device body, the outer surface of the simulation device body is rotatably connected with turnover observation window, the bottom of the simulation device body is fixedly connected with buffer rod by bottom mounting seat.The utility model is through the series connection effect of first spring damper, buffer spring and second spring damper, device forms gradient shock-absorbing network in three-dimensional space, horizontal direction impact is preferentially absorbed by first spring damper and converted into heat energy dissipation, vertical load is guided to spiral spring by buffer rod and carried out flexible support, extend the action cycle to reduce peak acceleration, moving block linkage mechanism cooperates second damper to realize the vector decomposition of oblique disturbance, finally reduce the vibration amplitude at culture dish, provide nearly stationary growth interface for embryo cell division.

1. Name of the product in this design: Graphical User Interface for Image Capture in an Embryo Incubator for Electronic Devices. 2. Purpose of this design: An electronic device. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Purpose of the graphical user interface: A graphical user interface used for capturing and recording images of embryos in an embryo incubator. 6. Human-computer interaction method of graphical user interface: The main view is the main interface of the product, which can monitor the status of the equipment in real time; clicking on any empty chamber in the main view (such as chamber 9) will enter the details interface of that empty chamber, resulting in interface change state diagram 1; clicking "Assign Patient" in the upper left corner of interface change state diagram 1 will result in interface change state diagram 2; selecting the patient list on the right side of interface change state diagram 2 and then clicking "OK" in the upper right corner will result in interface change state diagram 3; clicking "Preview Image" on the left side of interface change state diagram 3 will automatically start capturing images, as shown in interface change state diagrams 4-5. After the void detection is completed, it will automatically jump to the void mark manual review page, where void marks can be removed or added, resulting in interface change state diagram 6; clicking "OK" in interface change state diagram 6 will jump to the void not recording page. The prompt page will display a new interface (Figure 7); clicking "OK, Reviewed" in Figure 7 will display a new interface (Figure 8); clicking "Adjust Image" in Figure 8 will display a new interface (Figure 9), initiating the alignment operation; clicking "Focus" on the right side of Figure 9 will display a new interface (Figure 10), initiating the focusing operation; clicking "Compare" on the right side of Figure 10 will display a new interface (Figure 11), initiating the focusing operation; clicking "OK" on the right side of Figure 11 will display a new interface (Figure 12), redirecting to the image quality review prompt page; clicking "OK" in Figure 12 will display a new interface (Figure 13), redirecting to the embryonic development timeline page; clicking "Start" at the bottom of Figure 13 will display a new interface (Figure 14), redirecting to the secondary confirmation page. In this design interface, "xx" represents text and / or numbers and / or letters and / or symbols.

1. The name of the design product: embryo incubator image preview graphical user interface of electronic equipment. 2. The use of the design product: an electronic device. 3. The design points of the design product: in the graphical user interface. 4. The picture or photo that best indicates the design points: front view. 5. The use of the graphical user interface: for image preview display of embryos in the embryo incubator. 6. The human-computer interaction mode of the graphical user interface: the front view is the product main interface, which can monitor the real-time state of the recording cavity area, and click the corresponding area to view the details. The "xx" in the design interface represents text and / or numbers and / or letters and / or symbols.

The application discloses application of sodium crotonate in improving bovine embryo development potential. In the process of culturing bovine SCNT embryos in vitro, adding sodium crotonate (Nacr) with a proper concentration can promote the development of bovine SCNT cells, significantly improve the development rate and blastocyst rate of the SCNT embryos, improve the embryo quality, and further improve the somatic cell nuclear transfer efficiency of pre-implantation embryos. On this basis, the inventors construct a bovine SCNT early embryo in-vitro culture system, optimize the formula of the in-vitro embryo culture solution and the concentration of Nacr, and further establish a method for improving the development potential of early bovine embryos in vitro. The application can improve the development quality of bovine SCNT embryos, provides a research basis for the rapid propagation of high-yield dairy cattle, high-quality beef cattle and even disease-resistant fine breeds, provides a reference for the batch production of high-quality bovine embryos in vitro, and has important practical significance in the field of SCNT embryo in-vitro culture.

This invention relates to a blockchain-based method and system for visual management of embryo culture images, specifically in the fields of medical image processing and blockchain evidence storage. The method involves acquiring time-series images at a preset sampling frequency and extracting the digital digest hash value of the second frame. Boundary constraint processing is applied to the two frames to obtain target region feature images. Morphological variation feature values ​​are obtained through similarity analysis. If the mutation condition is not met, the on-chain rights confirmation request is blocked, and the digital digest is concatenated with the historical root hash value from the local cache pool to generate the target cumulative root hash value. If the mutation condition is met, the target cumulative root hash value is generated by packaging it with the digital digest and submitting it to the blockchain node for on-chain consensus rights confirmation. The sampling frequency and mutation condition are updated based on feedback and optimized parameters. This method addresses the systemic technical defects of existing solutions, such as on-chain resource depletion and severe delays in keyframe anchoring.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

This invention discloses a combination of exosomal miRNA biomarkers for single embryo culture medium used to assess embryo implantation potential, its application, and a method for assessing embryo implantation potential, relating to the field of assisted reproductive technology. The biomarker combination includes the miRNA molecular markers hsa-miR-143-3p and hsa-miR-125a-5p, wherein the nucleotide sequence of hsa-miR-143-3p is 5′-UGAGAUGAAGCACUGUAGCUC-3′, and the nucleotide sequence of hsa-miR-125a-5p is 5′-UCCCUGAGACCCUUUAACCUGUGA-3′. This invention establishes a non-invasive method for detecting embryo implantation potential, revealing significantly elevated expression of hsa-miR-143-3p and hsa-miR-125a-5p in non-pregnant EVs. Experimental data show that the expression levels of hsa-miR-143-3p and hsa-miR-125a-5p are significantly correlated with embryo implantation potential, serving as key indicators for assessing embryo implantation potential. This method can effectively distinguish between embryos with successful and failed pregnancies, providing new biomarkers for embryo implantation potential assessment. Compared with existing technologies, this method has advantages such as being non-invasive, highly specific, and having a clear mechanism, providing important technical support for improving the success rate of assisted reproductive technology and developing personalized treatment strategies.

The application discloses a trace nucleic acid multiplex detection method based on thermodynamic regulation and ultra-short amplicon design. The method is characterized in that the same probe binding region is designed on multiple primers, so that the same probe is targeted, thereby realizing the capture of multiple templates by a single probe. Meanwhile, the template binding region of the primer is designed differently according to specific detection sites to ensure site specificity. Through the above strategy, the problems of insufficient sensitivity, efficiency deviation caused by multiplex amplification primer competition and low detection rate of long amplicon design in the existing trace template detection technology are effectively solved. Through innovative primer structure design and reaction mechanism optimization, the application effectively improves the detection sensitivity and adaptability of fragmented nucleic acids while maintaining high multiplex detection capability. The method is especially suitable for the analysis of cfDNA, embryo culture solution free DNA and other low-abundance and fragmented templates, and has good clinical application prospect.

This invention discloses a rapid rice breeding method and its application based on a dominant extremely early-maturing gene. Using the dominant extremely early-maturing gene as the core, early-maturing single plants are selected as a backcross breeding bridge tool through maturity trait screening. The method includes: hybridizing rice materials carrying the target beneficial gene and rice materials carrying the extremely early-maturing gene as parents; obtaining an early-maturing bridge plant carrying the target beneficial gene through embryo culture, molecular marker screening, and maturity trait screening; using this early-maturing bridge plant as the male parent and continuously backcrossing it with the rice to be improved, conducting embryo culture, molecular marker screening, and maturity trait screening in each generation, compressing the single-generation backcross cycle to 60-76 days; self-purifying the last backcross generation, and obtaining improved lines through homozygous screening of the target beneficial gene, genetic background verification, and early-maturing trait removal. This invention utilizes the extremely early-maturing gene to accelerate generation iteration, enabling 4-5 generations of rice propagation per year, completing 3-4 backcrosses and 2 selfcrosses within 1.5 years, and can be applied on a large scale to improve multiple traits of rice.

The application discloses a visualized cattle and sheep embryo culture device and belongs to the technical field of cattle and sheep embryo in-vitro culture, which comprises an outer frame, the inside of the outer frame is provided with a culture layer, the inside of the culture layer is provided with a culture rack, the culture layer comprises a layered frame, the front end of the layered frame is embedded with a glass window, the bottom of the glass window is provided with a handle, and pull-out grooves are formed in the outer walls of the two ends of the layered frame. The device is characterized in that the layered plate sent by the alignment of the adjusting frame and the inclined mirror plate carried in each layered frame is aligned, the inclined angle of the inclined mirror plate is aligned with the glass window after being illuminated by the LED illuminating lamp, the user can conveniently observe the picture of the culture tank reflected on the inclined mirror plate through the glass window, the adjusting frame can be slid by rotating the threaded rod during the process, the observation position can be adjusted, and then the internal observation can be carried out without taking out the layered frame as a whole, so that the device is convenient to take and use and is more beneficial to cultivation observation.

The application belongs to the technical field of biology and particularly relates to a method for in-vitro rotation culture of embryos. The method places the embryos in embryo culture solution for culture, wherein the embryo culture solution comprises fresh rat serum, antibiotics, ascorbic acid or derivatives thereof, pyruvic acid or salts thereof, glutamine, buffer salt and optionally glucose; the embryo culture solution does not contain human umbilical cord blood serum; the use of human umbilical cord blood serum is avoided, so that the embryo rotation culture system can be quickly popularized; and further limitation is made to the use of fresh rat serum, adjustment of oxygen concentration at different periods, which can improve the proportion of embryos with continued blood flow in the yolk sac blood vessels at the E7.5+3 day period, realize the rapid blood flow in the yolk sac blood vessels of mouse embryos at the E7.5+5 day period, and complete the in-vitro culture process of mouse embryos from E7.5 to E7.5+6 day.

1. The name of the design product: embryo incubator of electronic device overview display graphical user interface. 2. The use of the design product: an electronic device. 3. The design points of the design product: in the graphical user interface. 4. The picture or photo that best indicates the design points: front view. 5. The use of the graphical user interface: for monitoring and managing the running state of the embryo incubator. 6. The human-computer interaction mode of the graphical user interface: the front view is the product main interface, the device can be monitored in real time, and the details state can be viewed by clicking the corresponding area. The "xx" in the design interface represents text and / or numbers and / or letters and / or symbols.

This utility model belongs to the field of embryo tissue culture technology, specifically relating to an intelligent sterile airflow barrier device for an embryo culture chamber. It includes a culture chamber with a vertical plate on the front and a horizontal plate on the top. The top edge of the vertical plate is fixedly connected to one side of an arc plate, the other side of the arc plate is fixedly connected to one side of the horizontal plate, the other side of the horizontal plate is fixedly connected to one side of an inclined plate, and the other side of the inclined plate is fixedly connected to the back of the culture chamber. This utility model can continuously blow clean the hands of operators when opening and closing the culture chamber door, effectively preventing contaminated air from directly entering the chamber; it can also effectively clean residual contaminants in the chamber cavity caused by eddies or dead corners; and it can save energy and extend the service life of the silent fan.

1. Name of the product in this design: Graphical User Interface for Editing Embryo Culture Parameters of Electronic Device. 2. Purpose of this design: An electronic device. 3. The key design feature of this product is its graphical user interface. 4. The image or photograph that best illustrates the design's key features: the front view. 5. Purpose of the graphical user interface: Used to edit the culture parameters of the embryo incubator. 6. Human-computer interaction method of graphical user interface: The main view is the main setting interface; click "Edit culture parameters" on the left side of the main view to get interface change state diagram 1; click the pencil-shaped edit icon to the right of the temperature of any chamber (such as chamber 1) in interface change state diagram 1 to enter the temperature editing mode and get interface change state diagram 2; click "Save" in the lower left corner of interface change state diagram 2 to get interface change state diagram 3; when you press the button pointing to the top of the chamber according to the page operation, you will get interface change state diagram 4; after successful setting, you will get interface change state diagram 5. In this design interface, "xx" represents text and / or numbers and / or letters and / or symbols.

The application provides a pretreatment liquid for bovine in-vitro embryo programmed freezing and a programmed freezing method, and the pretreatment liquid comprises an embryo culture liquid and 2-10 mu g / L cytochalasin B and 0.2-1.5 mM 4-phenylbutyric acid. The freezing liquid comprises the following components: 0.05-0.25 mg / mL hyaluronic acid, 2-20 uM astaxanthin, 0.01-0.12 mg / mL L-carnitine, 33.8-34.5 mg / mL sucrose, 0.3-2.0 mg / mL glucose, 5-20 %v / v ethylene glycol, 3-15 mg / mL bovine serum albumin and 25-45 mg / L sodium pyruvate PBS solution. The application comprehensively solves the in-vitro embryo freezing damage repair problem from two aspects of before freezing and during freezing, so that the in-vitro embryo is frozen by using the programmed freezing mode, and ideal thawing recovery rate and pregnancy rate are achieved, the industry problem is solved, and the development and application of the in-vitro embryo production technology are promoted.

The application relates to a purification method of chicken embryo culture influenza virus, and belongs to the technical field of biological medicine. The method is specific capture of inactivated virus liquid from chicken embryos through microspheres; the microspheres swell and adsorb viruses at low temperature, the network structure of the microspheres is triggered to shrink through temperature control, complete virus particles are released, and damage caused by chemical competition elution is avoided; the released viruses are lysed, and then purified through sucrose density gradient centrifugation and molecular sieve chromatography. Through the synergistic effect of impurity removal and specific capture and mild release, the application reduces ovalbumin residues, maintains virus hemagglutinin, and provides an industrialized feasible path for preparation of high-purity and high-immunogenicity influenza vaccine antigen.