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Micropropagation method for rubber tree good variety somatic embryo plant

An excellent variety, rubber tree technology, applied in the field of tissue culture and rapid propagation of agricultural biotechnology

Inactive Publication Date: 2013-06-05
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fast-propagating variety (Reyan 7-33-97) produced by the Rubber Institute of South China Academy of Thermal Sciences is a high-yield and wind-resistant variety, which is not suitable for the rubber planting area in Yunnan Province.
In addition, the tissue culture of rubber tree is one of the most difficult tree species in woody plants, and there are large differences in the difficulty of tissue culture among species

Method used

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Examples

Experimental program
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Embodiment

[0023] The somatic embryo plant micropropagation method of fine varieties of rubber tree, the concrete steps are:

[0024] 1 Bud induction process:

[0025] (1) The anther somatic embryo plant of Yunyan 73-477, a high-yield rubber tree variety Yunyan 73-477, which is in the test-tube seedling stage, is used as explants;

[0026] (2) Under aseptic conditions, remove the leaves of the somatic embryo plants, cut them into stem segments with 1 to 2 axillary buds or shoot tips of about 1 cm in length, and insert the rest into the induction medium upright. , the insertion depth is about 0.5cm for the stem segment, about 0.3cm for the tip, about 0.4cm for the base, and the top end of the stem is upward. The active ingredients of the medium are improved MS (reducing the macroelements of the MS basic medium to 80%, and the rest remain unchanged) and additional ingredients, the additional ingredients are 6-BA 0.5-1.0mg L -1 , NAA0.2~0.25 mg·L -1 , sucrose 60g·L -1 , the pH value was...

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Abstract

The invention discloses a micropropagation method for a rubber tree good variety (73-477 developed by Yunnan province) somatic embryo plant self-cultivated in Yunnan province. According to the method, a somatic embryo plant which is induced by anther or ovule of a rubber tree of a good variety 73-477 developed by Yunnan province is directly used and is cultivated to a budling in a juvenile form through micropropagation technology. The technological process includes an induction process of a bud, a multiplication subculture process of the bud and an elongation strong seedling cultivation process of the bud. The method is simple in technological process and low in production cost, and the whole technological process can be finished in the artificial environment and is free from influences of the season and the weather. The cultivated budling in the juvenile form has a plurality of bud eyes and a plurality of usable bud points, and the propagation speed is higher than that of the budling propagated through normal production. The micropropagation method provides a large amount of high-grade scions of self-cultivated good variety 73-477 developed by Yunnan province and is beneficial to popularization of the variety. Therefore, the micropropagation method has great application value.

Description

technical field [0001] The invention belongs to the field of tissue culture and rapid propagation of agricultural biotechnology, and in particular relates to a micropropagation method for an excellent rubber tree variety Yunyan 73-477. Background technique [0002] Natural rubber is one of the four major industrial raw materials and an extremely important strategic material. Natural rubber is a renewable and non-polluting resource, but it has become a constrained resource due to the limitation of the geographical distribution of rubber trees and their irreplaceability. Therefore, the total output of natural rubber is far from meeting the needs of the rapid development of the world economy. Since 2003, my country has become the world's largest consumer and importer of natural rubber. By 2010, the self-sufficiency rate was less than 25%. Yunnan is the earliest place to introduce natural rubber in my country, and it is also one of the major rubber planting provinces in my cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁国平黄凤翔管艳田海李玲孙小龙
Owner YUNNAN INST OF TROPICAL CROPS
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