114 results about "Micropropagation" patented technology

The present invention relates to an excellent bud strip for cultivating rubber bud grafting trees, seedling rootstock and grafting technology thereof, in particular to a method for seedling bud grafting of rubber tree micro-bud strips, the invention adopts anther culture combined with in vitro micropropagation technology The cultivated rubber micro-bud strips have a diameter of only 0.2 cm, small bud eyes, dense side buds, and high bud utilization. When budding, the xylem of the bud sheet does not need to be stripped off, and it is used for budding with rubber seedlings before the leaf-expansion stage as the rootstock, and the budding speed is fast. The juvenile budding tree grown by budding with this kind of bud strips grows faster , Higher output. The seedling budding method provided by the micro-bud strips of the rubber tree of the present invention is a juvenile clone cultivated by the micro-bud strips for the seedling bud-grafting, which not only achieves the purpose of shortening the cultivation period of the rubber tree bud-grafting seedlings, but also has a better effect than the existing rubber tree seedling budding. The cultivated old clone can increase the dry rubber output by 10-30%, and the growth speed can be accelerated by about 5%, and can be cut and put into production half a year earlier.

The invention relates to a method for producing sweet potato detoxified seedlings in a water culture manner. Healthy and robust detoxified seedlings are obtained by adopting an improved MS minimal medium and a greenhouse hydroponic micropropagation method. The requirements of soilless cultivation ways based on vermiculite, pearlite matrix and the like are met, so that healthy and efficient sweet potato seed production is carried out. By adopting the method, the original characteristic that the tissue culture seedling is free from viruses, bacteria and insects is kept, the traditional breeding technique is innovated, the growth speed of the detoxified seedlings is greatly accelerated, the breeding cycle is shortened, and the production cost is greatly reduced. The water culture seedling is simple in operation procedure, and the hydroponic seedlings can be produced within 4-6 months in a large scale according to the production requirements. Therefore, the problem that the detoxified seedlings cannot be quickly at low cost in a large scale by the traditional seedling cultivation method is solved.

The present invention provides methods for the micropropagation of reed grasses, particularly Arundo donax. In addition, the present invention provides methods for the macropropagation of bamboo and reed grasses, which utilize a unique float bed system and apparatus. The advantages of the present invention are that desirable plant clones can be obtained for planting in plantations and the methods are much more cost and labor efficient than traditional propagation techniques.

The invention discloses a novel rapid propagation technology in the technical field of biology, and relates to a method for the rapid propagation of dendrobium officinale kimura et migo under a non-aseptic condition. The method mainly comprises the steps of: non-aseptic germination of dendrobium officinale kimura et migo seeds, formation of protocorms and seedlings and successive rapid propagation under the non-aseptic condition. Coarse pine bark powder is added into a 1 / 2 MS culture medium to obtain a substrate free of sugar and agar, and the seedlings of the dendrobium officinale kimura et migo are placed into a closed container after being planted, are autotrophically grown through carbon dioxide under an approximately natural condition, and are promoted to be rooted, grown and strengthened within a shorter time. The method has the characteristics that seedling culture time is shortened, high-pressure sterilization is not required during successive culture, the seedlings can be propagated and grown in a normal state, and are difficult to pollute, and the cultured seedlings have large leaves and thick stems, and are high in quality. Compared with the conventional tissue culturing method for the dendrobium officinale kimura et migo, the method has the advantages that energy consumption is reduced, labor cost is reduced, and the method has the three major advantages of high speed, energy saving and good effect.

The invention relates to a micropropagation method for castanopsis hystrix twigs. The micropropagation method comprises the following steps: conventionally accelerating germination of high-quality seeds of castanopsis hystrix of more than 20 years old, and transplanting into a container cup for seedling cultivation; transplanting seedlings capable of receiving complete illumination onto an elevated seedling culturing bed containing base materials in a plastic greenhouse for seedling cultivation; cutting tops to promote sprouting, collecting lateral shoots, and transplanting into a container cup containing base materials in a cuttage way; and placing the container cup on the elevated seedling culturing bed in the plastic greenhouse for seedling cultivation to obtain container cuttage seedlings of castanopsis hystrix. By adopting the elevated seedling culturing bed and the seedling culturing base materials taking yellow soil as the main part to plant parent seedlings, water and nutrition conditions can be favorably controlled, and high-quality cut shoots can be cultured; meanwhile, when an air temperature and humidity regulator of the plastic greenhouse is used to strictly control the relative humidity and temperature of the air in the seedling culturing bed, the cuttage survival rate of castanopsis hystrix reaches more than 90-97%, and each parent seedling can produce 20-40 shoots. The method for culturing cuttage seedlings of castanopsis hystrix is convenient to operate, has low production cost, and can be used for rapidly culturing a large amount of high-quality asexual seedlings for forest planting, thereby having favorable market application prospects.

The present invention relates to an efficient method of in-vitro micropropagation of Piper longum plants. In particular, the present invention is directed towards the novel method for micropropagation of Piper longum from lateral bud (meristematic) explant (starting material), by culturing the explants on different medias. This method results in mass production of the plant Piper longum in a short span of time.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

The invention provides a seed-cultivating method of the blueberry by means of tissue cultivation and micropropagation, which comprises the following steps of: picking young sprout of the blueberry from fields in the growth season; sterilizing the surface of the young sprout under the aseptic condition; washing the sterilized young sprout with the sterile water; cutting the washed young sprout into a stem section with 1-2 bud(s); characteristically inoculating the stem section with 1-2 bud(s) on a first-generation inducting culture medium 3.0mg / L of WPM+ZT+0.02mg / L of NAA+30g / L of saccharose+6.5g / L of agar, wherein the PH value of the culture medium is 5.4; cultivating for 2-3 generations to obtain a tissue cultivation clone which is sterile and exuberantly grown and has multiple branches; using a cultivating culture medium 0.5-1.5mg / L of WPM+ZT+0.05-1.0mg / L of CPPU+30g / L of saccharose+6.5g / L of agar at the subculture and multiplication culture stage of the tissue cultivating seed of the blueberry, wherein the PH value of the culture medium is 5.4; and subculturing at a time every 50-60 days, thereby improving the propagation line by 5-10 times.

The invention discloses a clone micropropagation method for rubber tree, wherein self-rooting clone is obtained through mini-body breeding of rubber tree buds, the process consists of budling choosing, processing, inducement bud culturing and breeding, root growing. The breeding method has the advantages of simple process flow, low cost of manufacture, and high regeneration rate.

The invention relates to a plant tissue culture method, in particular to a tissue culture method of toxicodendron vernicifluum, and particularly relates to a high-efficiency and rapid micropropagation method for toxicodendron vernicifluum. The high-efficiency and rapid micropropagation method for toxicodendron vernicifluum comprises the following steps: (1) selecting and disinfecting an explant; (2) starting culture; (3) conducting enrichment culture; (4) conducting rooting culture; and (5) hardening seedlings and transplanting. The toxicodendron vernicifluum has the advantages of being stable in inheritable character, simple in culture process, easy to root, short in culture micropropagation period, and easy to survive after being transplanted. By adopting a method for conducting tissue culture on excellent single plants of toxicodendron vernicifluum, the propagation coefficient can achieve four to five times, the annual propagation amount of every ten effective toxicodendron vernicifluum axillary buds can still achieve more than 1.75 million calculated according to the propagation coefficient of 4.5 and the propagation period of 45 days despite pollution loss, the rapid propagation problem of the toxicodendron vernicifluum can be effectively solved, the excellent clone materials can be popularized on a large area, and foundation is laid for the industrial seedling.

A method of plant micopropagation is provided whereby a thin section of non-callus tissue obtained from a monocotyledonous plant, such as sugarcane, wheat or sorghum, is cultured in the presence of a cytokinin and / or an auxin. Optimal regeneration occurs when a basal surface of the thin section is oriented so as to be substantially not in contact with the culture medium. This micropropagation method produces mature monocotyledonous plants by either organogenic or embryogenic regeneration without substantial callus formation. By avoiding use of callus as a starting tissue, this method reduces the likelihood of propagated plants displaying somaclonal variation.

The invention discloses a method for constructing garlic micropropagation by taking bulb sheets as explants. Garlic bulb sheets are taken as explants; MS+1.5mg / L 2-D+0.5mg / L KT is taken as a callus induction and subculture medium; MS+5mg / L 6-BA+1.0mg / L NAA is taken as an adventitious bud differentiation induction medium; a bud strengthening culture process by taking MS+1.0mg / L KT+1.0mg / L GA+0.2mg / L NAA+2.0mg / L 6-BA as a bud strengthening culture medium is additionally added; a test tube seedling culture system taking a basic MS culture medium as a rooting culture medium is also additionally added. Test tube plants are subjected to hardening twice in indoor and field arch sheds, are transplanted into soil before winter, and regenerated underground bulbs with the sectioning rate being up to 94% are harvested in the next year. The technical system provides important guarantee for expanding propagation of high-quality garlic germplasm.

The present invention relates to a micropropagation method of purple-leaf birch, and relates to a micropropagation method. The invention settles the problems of long breeding cycle, long propagation efficiency, high cost and low survival rate. The method of the invention comprises the following steps: 1. sterilizing the explant; 2. cutting the explant to stem segment, and then executing induction cultivation for obtaining micro-branch; 3. cutting the micro-branch to stem segment and proliferating in the culture medium; and 4. executing invigoration culture to the proliferated micro-branch, sticking into a rooting substrate for executing rooting culture for finishing the micropropagation of purple-leaf birch. The micropropagation method of purple-leaf birch of the invention has the advantages of short propagation period, high efficiency and excellent genetic stability. The propagation efficiency is that the purple-leaf birch propagates for 30-40 times every 30-40 days. The method of the invention also has the advantages of simple operating technique, labor saving, time saving and low cost. The cost is saved by 30%-50% compared with the prior art. Furthermore the rooting rate and survival rate of seedling propagation equally can obtain 100%.

The invention discloses a technology for preparing bio-fertilizer from garbage. The technology provided by the invention adopts raw materials of 45% of garbage, 35% of turf, 13% of rice husk powder, 5% of danty, 1.5% of rice bran and 0.5% of a composite microbial inoculant, and comprises carrying out composting through uniformly mixing the crushed garbage, the crushed turf, the crushed rice bran and the crushed danty to obtain mixed raw materials, carrying out micropropagation of the composite microbial inoculant which is a home-made composite microbial inoculant, uniformly mixing the composite microbial inoculant subjected to micropropagation and the mixed raw materials, and piling up the mixture by a turning ventilation method so that the mixture forms a pile having the length of 5m, the width of 4m and the height of 1.5m. The technology provided by the invention selects a novel strain, utilizes novel inoculants, optimizes production processes, realizes preparation of organic bio-fertilizer satisfying the national standard through selection of a raw material ratio, confirms field application effects of the organic bio-fertilizer, lays a firm foundation for popularization of the organic bio-fertilizer and wide application of the organic bio-fertilizer in production, and is suitable for the technical field of environmental protection.

The invention belongs to the technical field of plant seedling propagation and discloses a micropropagation method of tilia miqueliana. The method comprises steps as follows: A, culture of seedling of the tilia miqueliana; B, adventitious bud induction; C, high-voltage electrostatic field processing; D, subculture and rooting; E, acclimation and seedling hardening. A large quantity of tilia miqueliana explants easy to disinfect can be obtained, the success rate of primary culture is higher than 98%, the proliferation rate reaches 113 times, the survival rate reaches 95%, the problem of shortage of the tilia miqueliana in the market is effectively solved, and the application prospect is wide.

Disclosed herein are media, systems and methods for achieving micropropagation of monocotyledonous plants.

The invention discloses a xanthoceras sorbifolia culture method, which relates to the xanthoceras sorbifolia rapid propagation technology and belongs to the technical field of plant tissue culture. The xanthoceras sorbifolia culture method solves the problem of phenomenon of one fruit of thousands of flowers of the xanthoceras sorbifolia. Therefore, the xanthoceras sorbifolia has high seedling differentiation rate, high propagation speed and high seedling yield. The xanthoceras sorbifolia realizes the micropropagation at the speed of several times each month, and the resource regeneration and the utilization of the micropropagation are reached through tender stem or stem tip culture. The xanthoceras sorbifolia culture method has the beneficial effects that the seedling development is healthy and strong, the planting survival rate is high, the seedling raising is early, the growth period is long, the seedling lignification degree is improved, the seedling cold resistance and overwintering capability is enhanced, the seedling raising time is short, the cost is low, and the seedling yield in the unit area is high.

The invention discloses a method for efficiently regenerating a Sapium sebiferum leaf disc. The method comprises the following steps: cutting leaves of aseptic Sapium sebiferum seedlings into leaf discs, and conducting induction culturing on the leaf discs through a cluster bud induction culture medium to form cluster buds; conducting micropropagation and elongation on the cluster buds in a micropropagation and elongation culture medium; and after the buds grow to 2-3cm, transferring into a rooting culture medium for rooting culturing, thus obtaining strong Sapium sebiferum test-tube plantlets after 25-30 days. Compared with the prior art, the method has the following advantages that raw materials are easily available and abundant, the regeneration efficiency is high, the culture period is short, hormone components added to the culture mediums are simple, the operation is convenient, and the like, and the method can realize fast propagation of excellent wild single plants, and keep the germplasm characteristics of a female parent, thus realizing the breeding of variety, laying a foundation for the genetic transformation of Sapium sebiferum, being beneficial to quickening the genetic improvement of Sapium sebiferum, and providing technical support for the fast propagation of excellent anaphase Sapium sebiferum plant systems in the later period.

The present invention relates to novel method for micropropagation of tea plants from explants obtained from completely folded, half opened or fully expanded leaves via callus phase, by culturing the explants on different media.

The invention relates to a method for micropropagation of arbuscular mycorrhizal fungi and belongs to the technical field of micropropagation of mycorrhiza fungi. The method for micropropagation of the arbuscular mycorrhizal fungi includes the following steps that firstly, the arbuscular mycorrhizal fungi are inoculated into a substrate to be cultivated, wherein the substrate comprises zeolite, pumice, vermiculite and peat; secondly, corn, marigold and clover are sequentially sown in the substrate in the first step, wherein the sowing density of each kind of plants is independently 8-12 seeds per square meter; thirdly, the sowing time of the corn serves as the reference, after the plants grow for 4-6 months, underground mycorrhizas are dried and smashed to be mixed with the substrate for cultivating the arbuscular mycorrhizal fungi. The method is high in mycorrhiza infection rate, high in fungus yield and good in micropropagation effect.

The present invention provides media, kits, systems, and methods for achieving large scale pistachio production within a short time via bioculture, large scale yam production within a short time via bioculture, high multiplication rate of plants including cannabis via in vitro micropropagation, high induction rates of somatic embryos from later buds in bamboo, reduced production of phenolic compounds in plants, high production of virus-free plants, including potato, and large scale hemp production via culturing. The present invention for pistachio and yam production results in shorter tuber development phase and higher yield. In some embodiments, the present invention provides compositions, methods, and systems for the micropropagation and mass production of perennials, grasses, bamboos, cannabis and phyto-pharmaceutical plants as well as hemp plants. In some embodiments, the present invention provides compositions, methods, and systems for reducing the production of a phenolic by a plant, such as bamboo.

The invention discloses a water planting cutting propagation method for myrtus communis. The method includes the steps of collecting and treating a cutting slip, preparing a nutrient solution, preparing a cuttage container, conducting water planting cultivation, conducing management after cuttage and conducting acclimatization and transplant. The method includes the specific steps that a semi-lignified myrtus communis tender branch is selected, and then is inserted in the cuttage container filled with the nutrient solution for water planting cultivation after being subjected to trimming treatment, the culture condition is controlled, the acclimatization and transplant are conducted after the cutting slip growing into a young root is obtained, and accordingly a myrtus communis nursery stock is obtained. By the adoption of the method, female plants can be accumulated, so that mass micropropagation is condcuted. Meanwhile, the problem that cuttage seasons are limited by time is solved, cutting propagation can be carried out all the year around, the culture period is short, rooting is fast, environment controllability is good, the culture condition is stable, and the survival rate of cutting slips can reach 93%. The method is suitable for mass production and can be widely popularized, and good economic benefits, social benefits and ecological benefits are achieved.

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a HDT date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a Black Barhee date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a T-R date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.

Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.

The invention relates to a thermo-photo-bioreactor and to a method for the culture and mass micropropagation of Deschampsia antarctica in vitro. The invention comprises a discontinuous immersion reactor for biomass micropropagation, including means for incorporating chemical inducing agents (salt, metals, organic compounds, etc.) and internal luminescence or illumination means (UV radiation and temperature) for supplying said chemical inducers and / or illumination during any growth phase of the vegetable or plant material (multiplication or propagation and / or maintenance). The invention is advantageous in that it can be used to produce large quantities of biomass of the aforementioned Antarctic species, while providing conditions suitable for said plant to produce metabolites that can be used for human health and personal care.