9 results about "Micropropagation" patented technology

A method is disclosed for synthesizing Ethephon-THAMs and using it as a controlled ethylene-releasing agent in potato tissue culture. The invention enables selective enhancement of shoot or root development and improves micropropagation efficiency

A propagation stopper comprising a radiated hydrophilic matrix body comprising a growth medium with bound organic fibers, the matrix body being rewettable and having a moisture content of less than about 20% by weight based on the total weight of the body, as measured according to ASTM Test Method D2216, and being sterile, as measured according to ASTM Test Method D2216, and having a moisture content of less than about 20% by weight based on the total weight of the body. The number of aerobic bacteria measured by the number 4833-1 of the ISO test method is lt; 10 cfu / g.

Examples of an automated micropropagation system for plant tissue culture is provided. The system comprises a culture vessel that comprises a number of detachable culture items that are designed with a plurality of culture cells sized to accept a single plantlet. Such culture vessel is fed into the system to an automated gripper and cutter assembly that is configured to grip the plantlets, cut them and transfer them into a new culture vessel.

A micropropagation system (100) for improved propagation of strawberry plants, comprising: a surface sterilization chamber (102) comprising one or more ultraviolet lamps (102e) for sterilizing strawberry runner tip explants; a sterile culture production chamber (104) for culturing the sterilized explants in a controlled culture room in which a temperature of 22°C to 23°C and a photoperiod of 12 hours per day is maintained; a regeneration chamber (106) for a plurality of shoots for inducing shoot regeneration in the explants; a root formation chamber (108) for inducing root formation in the regenerated shoots; and an acclimatization chamber (110) for acclimatizing the rooted plantlets to ex vitro conditions.

A recombinant protein for use in a liquid culture medium for photo-autotrophic micropropagation of Cannabis sativa L. is disclosed. The recombinant protein comprises a fusion of a growth induction part and a uptake enhancement part. The growth induction part comprises an Arabinogalactan protein and / or a plant transcription factor associated with plant growth and development. The uptake enhancement part comprises a cell penetrating peptide sequence and a nuclear localization signal encoded by the peptide sequence SEQ ID NO 1.

The invention provides a cunninghamia lanceolata rapid propagation method using stem segments as explants, and belongs to the technical field of plant tissue culture, the method comprises the following steps: S1, using the stem segments of cunninghamia lanceolata as the explants, and inoculating the stem segments on a first culture medium for induction culture; s2, inoculating the adventitious buds obtained in the step S1 to a second culture medium for multiplication culture; s3, inoculating the adventitious buds obtained in the step S2 into a third culture medium for rooting induction culture; s4, transplanting the aseptic seedlings obtained in the step S3; wherein the first culture medium and the second culture medium contain 6-BA and IBA, and the third culture medium contains SNP and NAA. According to the method, the tender stem of the cunninghamia lanceolata is taken as the explant, a cunninghamia lanceolata aseptic seedling micropropagation seedling rapid propagation system is successfully established for the first time, the transplanting survival rate is extremely high, and a reliable way is provided for keeping the excellent characters of the cunninghamia lanceolata and rapidly propagating the cunninghamia lanceolata.

The invention discloses a method for establishing an Australian dendrobium nobile micro-propagation system through somatic embryogenesis, which comprises the following steps of: culturing pseudobulb serving as an explant to generate axillary buds, inducing and culturing to form embryogenic calluses, and further differentiating and culturing until the embryogenic calluses fall off naturally to establish a root system, so as to obtain the Australian dendrobium nobile micro-propagation system. The method disclosed by the invention has the advantages of low chimera generation frequency, large number of regenerated plants, low somatic cell mutation rate and the like, and an efficient technical path is provided for rapid in-vitro propagation of the Australian dendrobium nobile.

The invention relates to a micropropagation method for rapid propagation of hazelnut (Corylus avellana L.) plants by tissue culture, comprising special nutrient media developed for the propagation stage and a technique for rooting.

This process yields totipotent plant cells capable of regenerating plantlets, similar to propagation, in vitro micropropagation, and synthetic seed production. It leverages spray drying as a technology for preserving plant germplasm and as a novel method not currently available. The process begins with somatic cells in suspension and involves inducing friable embryogenic calluses from seeds, leaves, stems, roots, or any plant explant capable of generating cells in suspension, followed by obtaining the cell pack. This cell pack is mixed with encapsulating agents and then spray-dried. Finally, the spray-dried cells are rehydrated and reactivated to continue their morphogenic development, resulting in plantlets capable of acclimatizing to greenhouse and field environments.