Method for constructing garlic micropropagation by taking bulb sheets as explants

A technology for explants and garlic, which is applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of low survival rate and high cost of test-tube seedling reproduction.

Inactive Publication Date: 2015-05-27
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to obtain a garlic rapid propagation technology system to solve the problems of high cost of growing garlic with garlic cloves and low survival rate...

Method used

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  • Method for constructing garlic micropropagation by taking bulb sheets as explants
  • Method for constructing garlic micropropagation by taking bulb sheets as explants
  • Method for constructing garlic micropropagation by taking bulb sheets as explants

Examples

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Effect test

Embodiment 1

[0065] A method for constructing garlic micropropagation with bulb sheets as explants:

[0066] (1) Obtaining of explants:

[0067]Select garlic heads that grow robustly, have no pests and diseases, and naturally end dormancy, peel off the outer scale leaves, rinse with tap water for 20 minutes, soak in 0.1% (w / w) mercury liter for 10 minutes, rinse with sterile water for 3 times, and then rinse at 75% (w / w) Soak in alcohol for 5 minutes to kill surface bacteria, rinse with sterile water for 3 times, then blot dry with filter paper for later use;

[0068] (2) Garlic callus induction:

[0069] Cut garlic bulbs into 2 mm thick slices on a clean table, inoculate them into callus induction medium, and cultivate them in an artificial climate box: day temperature 25°C, night temperature 20°C, light intensity 1500 Lux, light time 14 h / d, tiny callus particles were formed on the explants after 6 days, and a large number of callus particles had been formed on the explants after 2...

Embodiment 2

[0082] A method for constructing garlic micropropagation using bulb sheets as explants:

[0083] (1) Obtaining of explants:

[0084] Select garlic heads that grow robustly, have no pests and diseases, and naturally end dormancy, peel off the outer scale leaves, rinse with tap water for 20 minutes, soak in 0.1% (w / w) mercury liter for 10 minutes, rinse with sterile water for 3 times, and then rinse at 75% (w / w) Soak in alcohol for 5 minutes to kill surface bacteria, rinse with sterile water for 3 times, then blot dry with filter paper for later use;

[0085] (2) Garlic callus induction:

[0086] Cut garlic bulbs into 2 mm thick slices on a clean table, inoculate them into callus induction medium, and cultivate them in an artificial climate box: day temperature 25°C, night temperature 20°C, light intensity 1500 Lux, light time 14 h / d, tiny callus particles were formed on the explants after 14 days, and a large amount of callus particles had been formed on the explants afte...

Embodiment 3

[0099] The comparative test results of the garlic rapid propagation technology system of the present invention and the conventional test-tube plantlet rapid propagation method are as follows:

[0100]

[0101]

[0102]

[0103] Conclusion: Compared with the technical method of conventional test-tube seedling cultivation to obtain regenerated bulbs, the present invention increases the strong bud cultivation process in the test-tube seedling induction stage; at the same time, the test-tube seedlings are trained twice in indoor and field sheds, and transplanted to In the soil, the first generation of regenerated underground bulbs was harvested in June of the following year. This method can achieve up to 94% splitting rate in the first generation to regenerate underground bulbs, which provides an important guarantee for the germplasm propagation of fine varieties of garlic.

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Abstract

The invention discloses a method for constructing garlic micropropagation by taking bulb sheets as explants. Garlic bulb sheets are taken as explants; MS+1.5mg/L 2-D+0.5mg/L KT is taken as a callus induction and subculture medium; MS+5mg/L 6-BA+1.0mg/L NAA is taken as an adventitious bud differentiation induction medium; a bud strengthening culture process by taking MS+1.0mg/L KT+1.0mg/L GA+0.2mg/L NAA+2.0mg/L 6-BA as a bud strengthening culture medium is additionally added; a test tube seedling culture system taking a basic MS culture medium as a rooting culture medium is also additionally added. Test tube plants are subjected to hardening twice in indoor and field arch sheds, are transplanted into soil before winter, and regenerated underground bulbs with the sectioning rate being up to 94% are harvested in the next year. The technical system provides important guarantee for expanding propagation of high-quality garlic germplasm.

Description

[0001] This patent is funded by the key project of Tianjin Agricultural Commission (201302030) and the application development research fund of Tianjin Normal University. technical field [0002] The invention belongs to the technical field of vegetable planting, and relates to a method for constructing garlic micropropagation by using bulb slices as explants. Background technique [0003] As a vegetatively propagated crop, garlic is mostly planted with garlic cloves as seeds. Problems such as high production costs, low reproduction coefficient, virus accumulation, and species degradation have seriously affected garlic production (Xu Peiwen, 2006). It is imminent to find techniques for rapid propagation, detoxification and germplasm genetic improvement of garlic. In the previous work, a variety of garlic rapid propagation and detoxification technology systems were constructed, such as using garlic young leaves, fully developed leaf root tips, stem tips, and inflorescence rac...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王振英任春雪王珍范宝莉刘晓颖彭永康陈宏
Owner TIANJIN NORMAL UNIVERSITY
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