247results about How to "High differentiation rate" patented technology

The invention discloses a bletilla seed tissue culture propagation method which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following six steps of seed introduction and sterilization, induction culture of protocorm, enrichment culture of protocorm, induction culture of multiple shoots, enrichment culture of multiple shoots, and rooting culture. The method has the beneficial effects that seedlings generated by adopting seed tissue culture is low in pollution rate, high in differentiation rate, stable in hereditary characters, and high in active substance content. According to the method disclosed by the invention, the tissue culture propagation period of bletilla is also greatly shortened, and the tissue culture period from seeding to rooting is less than four months, so that the production time is shortened, and the production cost is lowered.

The invention provides a method for rapid tissue propagation and breeding seedling of bletilla striata seeds. The method comprises the following steps: disinfection and sterilization of the seeds, seed germination and protocorm induction, protocorm propagation, cluster bud induction and propagation, rooting culture, seedling adaptation and transplantation. The method has the benefits that the seed tissue culture is adopted, and obtained seedlings are low in pollution rate, high in differentiation, and high in content of active substances; meanwhile, through the adoption of the method, the tissue culture and propagation period of bletilla striata is greatly shortened, and the tissue culture period from seeds to rooted seedlings is only about 4 months, so that the production time is greatly shortened, and the production cost is reduced.

Disclosed is an agent comprising a benzothiophene alkyl ether derivative represented by the general formula below or a salt thereof:wherein R1 and R2 independently represent at least one group selected from a hydrogen atom, a halogen atom, an alkyl group, an aryl group, an aralkyl group, an alkoxy group, an aryloxy group, an alkylthio group, an arylthio group, an alkenyl group, an alkenyloxy group, an amino group, an alkylsulfonyl group, an arylsulfonyl group, a carbamoyl group, a heterocyclic group, an amino group, a hydroxyl group, a carboxyl group, a nitro group, an oxo group and the like; R3 represents an alkylamino group which may be substituted or an amino or hydroxyl group which may be protected; and m and n independently represent an integer ranging from 1 to 6. The agent is useful as a neurogenesis inducer or a therapeutic agent for neuropathy.

The invention relates to a culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte, and aims to solve the problem that the prior art is low in differentiation rate. The culture method comprises the following steps: 1) performing separation of primary cells of adipose tissue-derived stromal cells; 2) performing amplification and passage of the adipose tissue-derived stromal cells; and 3) performing differentiation culture on P5-generation adipose-derived stem cells to chondrocyte, namely, adding the adipose tissue-derived stromal cells into a condition culture medium, namely, an adipose tissue-derived stromal cell chondrocyte differentiation culture medium, after P5 generation of passage, performing chondrocyte differentiation culture, replacing the medium of the cells every 3 days, observing the morphological change of the cells, and after 16 days of induction, performing Alcian blue dyeing, and identifying the chondrocyte differentiation situation of the adipose tissue-derived stromal cells. The result of Alcian blue dyeing identification shows that the chondrocyte can be formed, and the Alcian blue dyeing is positive. The culture method has the characteristics of being simple and feasible, short in induction time as the induction culture medium is a serum-free culture system, good in test repeatability and high in osteoblast differentiation rate.

The present invention discloses cotton tissue culturing process and its application in screening out high differentiation rate cotton line. The cotton tissue culturing process includes the following steps: 1. adopting sterilized leaf stem as explant and inducing callus in callus inducing culture medium; 2. completing the differentiating culture of the callus in differentiating culture medium; and 3. further culturing of the differentiated embryonic callus in embryoid germinating and seedling forming culture medium to obtain regenerated plant. The process has high differentiation rate, and may be applied in screening out high differentiation rate cotton line. In addition, the screened high differentiation rate cotton can raise the converting efficiency of agrobacterium mediating process and shorten the converting period greatly. The present invention is significant in tissue culture, selective breeding and variety improvement of cotton.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention discloses an agrobacterium-mediated jatropha curcas gene transformation method. A material after 1 / 3 to 1 / 4 of base is cut from the cotyledon of a mature seed of jatropha curcas is used as an agrobacterium infection receptor. The method comprises the following steps of: after co-culturing, inoculating the transformed receptor and the agrobacterium to a culture medium without a screening agent to perform callus induced culture for 4 weeks; and succeeding the receptor and the agrobacterium to a selective culture medium and screening resistant buds by using kanamycin sulfate as a screening agent, sub-culturing the resistant buds obtained by screening to obtain resistant plants, and obtaining transgenic jatropha curcas plants by molecular detection. Compared with the conventional jatropha curcas transformation method, the method can obtain high regenerative bud induction rate, simplifies the transformation program, shortens the transformation period, greatly improves the transformation efficiency and has good application prospect.

The invention discloses a special anti-cracking foliar fertilizer for a jujube tree, and a preparation method and an application thereof. The special anti-cracking foliar fertilizer for the jujube tree is composed of the following components in percentages by mass: 5% to 8% of potassium chloride, 7% to 15% of calcium chloride, 1% to 5% of borax, 1% to 5% of chelated magnesium, 1% to 3% of chelatediron, 0.5% to 1.5% of zinc sulfate, 0.2% to 0.6% of manganese sulfate and 3% to 4% of organosilicon, with the balance being water. The foliar fertilizer provided by the invention has low production cost, is safe and effective, and is beneficial for and harmless to human and livestock; mineral elements added in the foliar fertilizer are needed by animals, and can effectively prevent a variety of diseases like yellow-leaf disease and little-leaf disease caused by nutrient deficiency of a fruit tree; the disease resistance of a tree body is strengthened; the quality of a fruit is improved; the soluble sugar content of a fruit is improved by 14.3% to 43.5%, the weight of a single fruit is increased by 5% to 13.3%; the proportion of high-quality fruits is increased by 20.2% to 69.8%; the proportion of low-quality fruits is decreased by 28.9% to 76.9%; the fruit cracking rate of golden-silk jujube is significantly decreased by 30% to 45%; meanwhile, the contents of mineral elements in fruits and leaves are significantly improved; and the foliar fertilizer is free of any side effects, is safe and reliable, and is a green and environment-friendly product.

The invention provides a sexual propagation method of bletilla striata. The sexual propagation method of bletilla striata particularly comprises the steps of processing of capsules, preparation of nutrients, inoculation and cultivation, and bottle out domestication, and is characterized in that in the step of processing of capsules, an alcohol lamp drying mode replaces a filter paper sucking dry mode in the prior art; in the step of preparation of nutrients, combination of banana slurry and humus is adopted; in the step of inoculation and cultivation, only inoculation, germination cultivation and strong seedling cultivation steps are included, the conventional enlargement cultivation process in an existing tissue culturing technology is omitted, and the process is simplified and the cost is lowered while the purposes are achieved; in the step of bottle out domestication, cultivation is performed through humus in a greenhouse, domestication is finished under the condition of controlling the relative humidity, temperature and shading rate, and qualified bletilla striata seedlings are obtained. Bletilla striata seeds are used for scale seedling culturing, the survival rate is high, manual scale propagation of bletilla striata is achieved, and the obtained bletilla striata seedlings are low in contamination rate, high in differentiation rate and stable in inheritable character.

The invention relates to a mature wheat embryo culture and regeneration method. Regeneration culture mediums are based on MS basic culture mediums. In the regeneration culture mediums, the content of maltose is 40g / L, the content of MES is 1.95g / L, the content of KT is 5mg / L, the content of NAA is 0.1mg / L and the pH is 5.8. The specific method comprises the steps of selecting mature and full wheat seeds, soaking the seeds in 70% alcohol solution for 5min, sterilizing the seeds in 0.1% HgCl2 solution for 20min, washing the seeds for at least four times by using sterile water and soaking the seeds in the sterile water for 19-24h; removing the buds and the roots of the mature embryos of the seeds, longitudinally cutting the mature embryos, putting the cut mature embryos into induction culture mediums to culture in the dark at 23-25 DEG C, conducting subculture once for 10-14 days and conducting induction culture for totally 20-28 days to obtain mature wheat embryo calluses; and then transferring the mature wheat embryo calluses into the prepared regeneration culture mediums to culture in the light at 23-25 DEG C for 25-30 days and inducing the mature wheat embryo calluses to be differentiated to form regenerated seedlings.

The invention belongs to the field of biomedicine, and specifically relates to a method used for inducing differentiation of human multipotential stem cells into retinal progenitor cells. According to the method, human embryonic stem cells or human induced multipotential stem cells are induced in a medium containing N2, and then are delivered into a retina induction medium containing neural basis culture medium, knockout serum replacement and nicotinamide so as to obtain the retinal progenitor cells. The method is capable of increasing differentiation rate of human multipotential stem cells into retinal progenitor cells; and the retinal progenitor cells obtained via differentiation can be used for transplantation therapy of irreversible degenerative diseases of retinal neurons, such as age-related macular degeneration and retinitis pigmentosa, and also can be used for iPS cells sourced from patients as cell models in drug screening and gene interference experiments. Compared with existing technology, small molecule compounds used in the method are less, so that influences caused by non-anthropogenic factors are avoided effectively, and yield of obtained optic cup-like cells is obviously higher than that of existing induced differentiation methods.

The invention discloses a xanthoceras sorbifolia culture method, which relates to the xanthoceras sorbifolia rapid propagation technology and belongs to the technical field of plant tissue culture. The xanthoceras sorbifolia culture method solves the problem of phenomenon of one fruit of thousands of flowers of the xanthoceras sorbifolia. Therefore, the xanthoceras sorbifolia has high seedling differentiation rate, high propagation speed and high seedling yield. The xanthoceras sorbifolia realizes the micropropagation at the speed of several times each month, and the resource regeneration and the utilization of the micropropagation are reached through tender stem or stem tip culture. The xanthoceras sorbifolia culture method has the beneficial effects that the seedling development is healthy and strong, the planting survival rate is high, the seedling raising is early, the growth period is long, the seedling lignification degree is improved, the seedling cold resistance and overwintering capability is enhanced, the seedling raising time is short, the cost is low, and the seedling yield in the unit area is high.

The invention provides a cultivation method for high yield and high quality industrial cannabis sativa. The method mainly includes the steps of: 1. flower bud collection and screening; 2. flower bud sterilization and inoculation; 3. anther induction culture; 4. callus redifferentiation; 5. adventitious bud proliferation and rooting; 6. pollen plant ploidy detection, and rejection of non-diploid plants; 7. transplantation of pollen plant seedlings; and 8. cross breeding, thus obtaining a holandric new line. The invention for the first time establishes a complete system for industrial cannabis sativa anther culture, also utilizes the method to produce super-male plants and female plants, and the plants are subjected to cross breeding to cultivate the holandric new line, thus significantly improving the yield and quality of cannabis sativa fiber, and providing a material basis for the development and utilization of cannabis sativa and cannabis sativa fiber.

The invention relates to a method for picea balfouriana somatic embryo generation and plant regeneration, which comprises steps of collecting picea balfouriana open pollination clonal cones, and separating immaturate zygotic embryos out for somatic embryogenetic culture, wherein the somatic embryogenetic culture comprises four stages, i.e. an embryogenetic callus inducing stage, an embryogenetic callus multiplicating stage, a somatic embryo maturity stage and a somatic embryo germination stage. Compared with the prior art, the method has the advantages that the seedling stage is greatly shortened, and natural maturing rate, seed maturation rate, differentiation rate and germination rate are all improved. The seedling culture method having short period, high multiplying rate, closely linked cultivation steps and low cost is provided for popularizing and planting picea balfouriana in a large scale for forestry production, is suitable for a rapid propagation technical system of picea balfouriana cell engineering and satisfies the requirements of markets on picea balfouriana.

The invention relates to a bletilla lateral bud tissue culture propagation method, and belongs to the technical field of traditional Chinese medicine material plantation. The method comprises the following steps of introduction sterilization, bud sprouting culture, multiple shoot induction culture, multiplication culture and rooting culture. The lateral bud is adopted for tissue culture, so that the pollution rate of the obtained seedlings is low, the differentiation rate is high, inheritable character is stable, and the content of active substances is high; and meanwhile, the tissue culture propagation period of the bletilla is greatly shortened, the tissue culture period from the cutting of lateral bud to the rooting is less than 4 months, the production time is greatly shortened, and the production cost is reduced.

The invention belongs to the technical field of plant tissue culture, particularly relating to a rapid in-vitro propagation method for rhizome of flaccid anemone; the method comprises explant selection and sterilization of the rhizome of flaccid anemone, inducement and proliferation of the adventitious buds, pseudobulb inducement and proliferation, embryogenic callus inducement, proliferation, differentiation, rooting and other steps. The invention provides an explant aseptic sterilization and cultivation system for rhizome of flaccid anemone, a cultivation process, a special culture medium and other key techniques, and successfully produces the tube-cultivated plantlet of the rhizome of flaccid anemone. The invention overcomes the disadvantages of long breeding cycle and low breeding coefficient of the rhizome of flaccid anemone under natural conditions, and provides a practical technology for rapidly propagating tube-cultivated plantlets of the rhizome of flaccid anemone in an industrialized mode.

The invention discloses a Kyoho grape labor-saving cultivation method achieving production and sightseeing and picking. The method comprises various measures such as garden plot selection, canopy frame building, medium cultivation, ridging, field planting, water management, psi-shaped pruning, denuclearization treatment, soil management, anti-bird mesh covering, leaf picking, bagging and disease and pest control. According to the Kyoho grape labor-saving cultivation method achieving production and sightseeing and picking, a psi-shaped pruning mode is adopted to shape grapes, the method conforms to growth habits of the Kyoho grapes, a tree body reaches a balance of reproductive growth and vegetative growth, the flower bud differentiation rate, the fruit setting rate, the fruit sugar degree and the fruit yield are obviously increased, and the quality is obvious improved; mechanical picking can be conducted, the method is suitable for sightseeing and picking, and the method can be popularized in a large area in industrial production of the grapes.

The present invention relates to a culture media for peony high frequency embryogenic callus differentiation, and a culture method thereof. The culture method comprises steps of material selecting, seed embryo sterilizing and separating, culture media preparing, callus differentiation and embryo bud differentiation. The culture media for the peony callus differentiation is prepared by adding 0.5-2.0 mg / L of NAA, 0.1-0.5 mg / L of TDZ, 30 g / L of sucrose and 6 g / L of agar to a modified 1 / 2MS basic culture media, wherein the pH value is 5.8. The culture media for the peony embryo bud differentiation is prepared by adding 1.0-5.0 mg / L of TDZ, 100 g / L of sucrose and 6 g / L of agar to the modified 1 / 2MS basic culture media, wherein the pH value is 5.8. The culture media and the culture method of the present invention have the following advantages that: the raw materials are easily obtained; the callus rate is high; the browning rate is low; the embryo bud differentiation rate is high; the generesearch and the gene application requirements can be met.

The invention discloses a quick cultivation method for rhizoma bletillae tissue culture seedlings. The method comprises the following steps: selecting intact non-cracked rhizoma bletillae capsules and disinfecting the capsules by 75% alcohol for 30 seconds; then soaking and disinfecting the capsules by using 0.2% mercury bichloride for 10 minutes; washing 6 times by sterile water; fully and thoroughly washing, airing and uniformly spreading on a culture medium to cultivate; and when the seeds germinate to obtain seedlings which are about 2cm long, inoculating 3-4 seedlings as a cluster to a strong sprout culture medium to be cultured. Compared with the conventional tissue culture seedlings obtained by sterile germination of rhizoma bletillae seeds, the method just needs subculture once, so that the time consumed is short and at least three months are saved.

The invention discloses a tissue culture method for populus yunnanensis Dode with a tender stem as an explant, belonging to the tissue culture method for plants. The tissue culture method comprises the following steps of: acquiring populus yunnanensis Dode branches growing for three years; carrying out water planting and then taking the tender stem as the explant; flatly placing an aseptic tender stem in a differential medium; growing fasciculate adventitious buds on callus; and respectively connecting into a multiplying and rooting culture medium, transplanting a domestication matrix, and the like. According to the tissue culture method disclosed by the invention, materials can be conveniently and rapidly obtained, culture media and domestication matrixes at various phases are respectively screened and compared; and the invention provides a method for industrial rapid reproduction and seedling culture of the populus yunnanensis Dode as well as the research for physiologic genetic characteristic of the populus yunnanensis.

The invention discloses a monkshood-tuber tissue culture and rapid propagation method, which comprises the main steps of selection, disinfection and sterilization of an explant, inoculation and induction of callus, induction of cluster buds, root induction, hardening-seedling and transplanting and the like. By carrying out tissue culturing on stem tips, blades, roots, stem sections, leafstalks, seeds and the like of monkshood-tuber as the explants, and inducing a plant to regenerate and rapidly propagate monkshood-tuber seedlings, the inductivity, the differentiation rate and the rooting rate are higher, and the survival rate of the seedlings after being transplanted in culture soil is high. The method can effectively overcome the problems of poor disease resistance, low and unstable output, varietal complexity and the like of a traditional monkshood variety in the production of the monkshood-tuber, realizes the variety purification and rejuvenation through stem tip detoxification, tissue culture and other means, and provides excellent monkshood varieties, and lays a favorable foundation for the factory and scale production of large quantities of high-quality seedlings required by monkshood GAP (Good Agricultural Practices) bases and the like.

The invention discloses a tissue culture rapid propagation method of callicarpa nudiflora. The method performs control on the explant collection, disinfection method, differentiation, multiplication, screening of a rooting medium, selection of a transplanting matrix, environmental conditions in the culture process and the like. By adopting the methods of explant selection, inoculated culture, propagation expanding culture, rooting culture and hardening-seedling transplanting, a set of complete callicarpa nudiflora tissue culture rapid propagation technology system is established, so that the differentiation rate reaches 100%, the monthly multiplication coefficient reaches 4.95, the rooting rate reaches 93.7%, and the transplanting survival rate exceeds 90%. The method disclosed by the invention is simple and easy to implement, simple in process and high in efficiency, effectively suppresses the pollution and browning of the callicarpa nudiflora explant, improves the differentiation rate of the tissue culture seedling, realizes fast multiplication, high rooting rate, high survival rate and low cost, and provides a reliable technical means for large-scale production of callicarpa nudiflora seedlings.

The invention relates to a seed selection method for drought and heat resistant cymbidium hybridum. The method comprises the following steps: (1) carrying out the screening and parent selection on seed sources according to the standard of drought and heat resistance; (2) hybridizing the parents which have the drought and heat resistant characteristic and are selected in the step (1); (3) carrying out the seed asepsis culture; (4) carrying out the filial generation culture; and (5) carrying out the breed screening of the filial generation according to the standard of drought and heat resistance so as to complete the seed selection. The seed selection method for drought and heat resistant cymbidium hybridum can make full use of cymbidium hybridum resources at both home and abroad to culture a new cymbidium hybridum species with the proprietary intellectual property rights. The new cymbidium hybridum has the advantages of draught resistance, high temperature resistance, and adaptation to be implanted in North China.

The invention discloses a method for rapid tissue propagation of acer saccharum, and belongs to the technical field of woody plant tissue culture and propagation. The method comprises the following steps: selecting explants, pretreating the explants, establishing a sterile system, performing inductive culture, performing multiplication culture, performing rooting culture, strengthening and domesticating seedlings, and the like. Tissue organs of plants are cultured under a sterile system condition by controlling external environment conditions artificially, the purpose of rapidly propagating nursery stock is achieved, and stable properties of excellent female parents are effectively maintained. Compared with common vegetative propagation of cuttage and grafting, the method is small in material amount, free of limit of natural conditions and relatively applicable to neophytes, the propagation coefficient is as high as 5-20 times, and propagation and popularization problems caused by shortage of resources of the acer saccharum are solved.

The invention discloses a method of inducing the human embryo stem cell differentiation to liver cell and its special culture medium. The said culture medium comprises the following two culture mediums: differentiation medium I which is prepared by adding 80-120ng / activinA in RPMI 1640 culture medium and differentiation medium II which is prepared by adding 20-60ng / mL desmocyte growth factor and 15-25ng / mL bone formation protein in HCM medium. The culture medium is of positive components and high use safety; the revulsion is of short periodic time, high differentiation rate and stable safety, and has a wide application foreground.

The invention relates to a method for obtaining regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants. The method comprises the following steps: step of: (1) acquiring calluses of the dove tree leaves; (2) breeding the calluses; (3) inducing cluster buds; (4) breeding the cluster buds; and (5) inducing roots. According to the method, the environmental temperature in steps (1)-(5) are 22-26 DEG C, and the environmental humidity is preferably 60%-90%. According to the method, the differentiation rate of the calluses is higher and reaches more than 50%; the rooting percentage is 100%; and the survival rate of transplanting is more than 90%.

The invention discloses induction of tung tree hypocotyls callus and a method for efficiently regenerating plants, and belongs to the field of plant cell engineering. According to the invention, through the processes of inducing the callus by the hypocotyl of the tung oil tree, inducing an adventitious bud by the callus, performing transgenerational multiplication culture, performing strong seedling culture, performing rooting culture, performing acclimatization and transplantation and the like, a way for quickly breeding good tung oil tree plants is provided, and a firm foundation for later improving the resistance of the tung oil tree, accelerating the molecular breeding process of the tung oil tree, improving the oil quality and other properties, and establishing the genetic system of the tung oil tree in a genetic engineering method is built.

The invention relates to the technical field of cell induction culture, and particularly relates to a culture method for inducing the differentiation of an umbilical cord mesenchymal stem cell into acartilage, and a culture medium used in the method. The culture medium is obtained by at least additionally adding the following components in a basal culture medium: 7 ng / mL to 13 ng / mL of TGF (Transforming Growth Factor)-beta 3, 0.07 mumol / L to 0.13 mumol / L of dexamethasone, 40 mug / mL to 60 mug / mL of ascorbate, 0.7 w / v% to 1.3 w / v% of 1*ITS (Internal Transcribed Spacer), 30 mug / mL to 50 mug / mL of L-proline and 0.7 mmol / L to 1.3 mmol / L of sodium pyruvate. According to the culture medium, through optimizing an induction component and matching a specific culture method, the induction velocity is improved; the hard and compact cartilage can be formed after 7 days; all inoculated mesenchymal stem cells can be differentiated into cartilage cells, and the differentiation rate is improved. An induced cartilage globule is compact in texture, and displayed through specific staining, and all mesenchymal stem cells are sufficiently differentiated into the cartilage cells.

The invention discloses a method for fast propagation of test tube arrowhead by tissue culture. The test tube arrowhead is formed by sterilizing treatment of an explant, stem tip differentiation, subculture multiplication and induction. According to the method disclosed by the invention, the propagation of the test tube arrowhead is carried by tissue culture, and thus high propagation coefficient and short propagation period are realized; in addition, the whole propagation process is carried out under manual control conditions without being influenced by an external environment; the test tube arrowhead can be annually produced; due to small size and light weight, the test tube arrowhead can solve the problems existing in the remote transportation and storage of arrowhead test tube seedlings; the test tube arrowhead has no disease, so that the problem that a conventional arrowhead seed easily carries diseases is solved; the induction of the test tube arrowhead provides an operable simple system for research work of the carmus expansion mechanism; as an in-vitro dormancy organ, the in-vitro indoor preservation research on arrowhead germ plasm resources can be realized; and live exchange of international germ plasm resources is benefited. The invention provides a quick, convenient and effective propagation method for the development of the arrowhead industry.

The invention relates to a tissue-culture propagation method of cold-resistant Chinese rose and solves the propagation problems of slow propagation and low multiplication coefficient of the cold-resistant Chinese rose. The method includes the steps of firstly, screening explants; secondly, sterilizing the explants; thirdly, performing primary culture; fourthly, performing multiplication culture; fifthly, performing strong-seedling culture; sixthly, performing rooting culture; seventhly, transplanting and performing acclimation. The method is characterized in that newly grown top buds or lateral buds of plants are used as the explants, MS is used as the basic culture medium, naphthylacetic acid (NAA), 6-benzylamino purine (6BA) and indolebutyric acid (IBA) are utilized to perform hormonal regulation, and accordingly the regeneration system of the cold-resistant Chinese rose is built. The method has the advantages that one multiplication subculture cycle of the method is 4-5 weeks, multiplication times is controlled to be 6-8 times, the method is unaffected by seasons, seedling propagation coefficient can be increased greatly, a scientific and effective propagation method is providedfor the cultivation and application of the cold-resistance Chinese rose, production requirements are satisfied, industrial propagation can be performed, and reference can be provided for the propagation of other cold-resistant Chinese rose varieties.