6results about How to "High differentiation rate" patented technology

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

This invention provides a method for rapid propagation of *Sedum aizoon* via tissue culture, belonging to the field of plant tissue culture technology. The method includes the following steps: 1) Using *Sedum aizoon* stem segments as explants, the stem segments are disinfected, cut, and inoculated into a callus induction medium for callus induction culture to obtain callus tissue; 2) The obtained callus tissue is inoculated into a shoot induction medium for shoot induction culture to obtain adventitious shoots; 3) The obtained adventitious shoots are inoculated into a shoot proliferation medium for shoot proliferation culture to obtain regenerated seedlings; 4) The regenerated seedlings are inoculated into a rooting medium for root induction culture to obtain transplanted seedlings; 5) The transplanted seedlings are transplanted into soil for cultivation. The method provided by this invention can significantly improve the differentiation rate of callus tissue and the survival rate of regenerated seedlings.

The application belongs to the technical field of flower cultivation, and discloses a constant-temperature culture device for tulipa gesneriana and a culture method thereof. The constant-temperature culture device for tulipa gesneriana comprises a box body, double-layer box doors are mounted on the side of the box body, a plurality of supporting rods are mounted in the inner cavity of the box body, and a lattice plate is fixed to the side wall of each supporting rod. The cooperation of the structures such as the air diffuser plate, the ventilation pipe, the limiting rod and the air inlet joint improves the air exhaust uniformity and stability. When the temperature is controlled and adjusted, the air inlet hole groove on the air inlet joint is exposed, the air outside the air diffuser plate is introduced into the ventilation pipe, the air is uniformly exhausted from the air outlet on the ventilation pipe, and there are a plurality of equidistant air outlet points at the bottom of each layer of bulbs, so that the air exhaust uniformity and stability are effectively improved. When the temperature needs to be changed, the plurality of air outlets in the box body can synchronously blow out hot air or cold air, the response rate is greatly improved, and the flower bud differentiation rate is improved.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The present application belongs to the technical field of organoid culture, and particularly relates to a functional bovine mammary gland organoid as well as a culture method and application thereof. The present application overexpresses BCL2 a gene in bovine embryonic stem cells, improves early cluster culture cell cluster cluster activity and / or cell cluster survival rate; bovine embryonic stem cells overexpressing BCL2 a gene are subjected to 3D culture to obtain an organoid cell cluster; 60% Matrigel is used as a supporting matrix material to provide a 3D culture environment for the organoid cell cluster, which can not only make the organoid cell cluster sprout, but also control the elongation speed of the organoid cell cluster, form a tubular structure similar to mammary ducts, and obtain a bovine mammary gland organoid with a high mammary gland differentiation rate. The obtained bovine mammary gland organoid has the function of inducing lactation, and provides a reliable model for analyzing the mammary gland development process of dairy cattle and improving lactation of dairy cattle.