The invention discloses an experimental method for promoting animal
mammary gland development by using
vitamin B3. The method includes the following steps: S1, selecting 20
pregnant female mice, performing single culture, performing 12h:12h day and night discontinuous lighting, and performing
free feeding and drinking; S2, taking care born mice by mother mice for four weeks after pregnant mice delivery, performing artificial
weaning on the mice, and dividing the mice into two groups including a NT group (10) and a
vitamin B3 group (10), wherein the feeding conditions of the NT group remain unchanged, and 0.5% of the
vitamin B3 is added into daily feed of the
vitamin B3 group; and S3, after the mice are fed for three weeks, killing the mice by cervical
dislocation, and taking fourth pairs of mammary glands of the mice for
Whole mount staining. According to the method for promoting the adolescent
mammary gland development by using the
vitamin B3, through in-vivo and in-vitro experiments,the proliferation and
cyclin expression of EPH4EV cells and mouse mammary glands are detected, the
molecular biology is used to comprehensively evaluate the effects of the
vitamin B3 on the EPH4EV proliferation and
mouse mammary gland development, and results show that the vitamin B3 can significantly promote the expression of
cell proliferation markers in the cells and mouse mammary glands, thereby promoting the
cell proliferation and
mammary gland development.