14 results about "Weaning weight" patented technology

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to the weaning weight character of sheep, the molecular marker is a nucleotide sequence shown in SEQ ID NO.1, and nucleotide at the 101bp site is T or C; the application refers to any one of the following (1) and (2): (1) identifying the weaning weight of sheep; and (2) the weaning weight of the offspring of the sheep is improved. The screened molecular marker influencing the weaning weight character of the sheep can be used for identifying the weaning weight character of the sheep, and the weaning weight of offspring can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention relates to the field of breeding feed, and provides a formula of fermented feed for feeding two-end black pigs, and the fermented feed comprises the following preparation materials: 150-220 parts of ice clara; 100 to 180 parts of dried ear grains; 0-30 parts of moso bamboo powder; 0 to 100 parts of straw powder; 200 to 300 parts of rice bran; 180 to 250 parts of corn flour; 90 to 180 parts of soybean meal; 10 to 5 parts of pine needle meal; and 5-10 parts of a fermentation strain. The fermented feed in the formula of the fermented feed for feeding the two-end black pigs is rich in probiotics and organic acid, the intestinal microecological balance can be improved, the weaning litter weight and the weaning weight gain of piglets can be remarkably improved through the EM bacteria fermented feed, meanwhile, the daily average feed intake of sows is remarkably improved, and the diarrhea rate of the piglets is remarkably reduced.

This invention relates to the field of genetic breeding technology, specifically to the application of a molecular marker related to the pre-weaning weight gain trait in sheep. The molecular marker is at least one of the following ①~②: ① The nucleotide sequence shown in SEQ ID NO.1, where the nucleotide sequence at the 101bp site is T or C. ② The nucleotide sequence shown in SEQ ID NO.3, where the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) Identifying the pre-weaning weight gain in sheep. (2) Improving the pre-weaning weight gain of sheep offspring. The molecular markers screened by this invention that affect the pre-weaning weight gain trait in sheep can be used to identify the pre-weaning weight gain trait in sheep. By selecting individuals with the CC genotype as the father or mother, the pre-weaning weight gain of offspring can be improved.

The invention is applicable to the technical field of feeds, and provides a special nutrient enrichment premix for boars and a preparation method thereof. The compound feed additive is prepared by compounding beta-carotene, 25-hydroxyvitamin D3, dl-alpha-tocopheryl acetate, menadione sodium hydrogen sulfite, ammonium sulfate nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamin, nicotinamide, D-calcium pantothenate, folic acid, D-biotin, L-ascorbic acid, DL-methionine, zinc methionine chelate, selenium yeast, chromium picolinate, L-carnitine and glucose according to a specific proportion. A dynamic batching and high-precision mixing process is adopted for preparation. Through the synergistic effect of various nutrients, the sow milk quality can be remarkably improved, the birth weight of piglets is increased by 50 g or above, the survival rate is increased by 6-8% or above, the weaning weight is increased by 500 g or above, meanwhile, the sow estrus rate is increased by 5% or above, the problems that sows are poor in body condition, low in reproductive performance, low in piglet survival rate and the like in existing boar breeding are effectively solved, and extremely high breeding application value is achieved.

The application belongs to the technical field of composite materials, and particularly discloses a preparation method and application of a stress-resistant and immunity-enhancing preparation for piglets, which is prepared by using sodium iron chlorophyll and sodium selenium-rich acid as core blood supplement elements, collocating an astragalus polysaccharide and a cordyceps polysaccharide compound polysaccharide system, combining functional oligosaccharides, antibacterial peptides and probiotics to construct an intestinal microecological regulation module, and supplementing with composite amino acids and vitamins to form a nutritional synergistic system, and is prepared through gradient sugar molecule inclusion, low-temperature microcapsule embedding and other innovative processes, so that the physical energy, qi and blood are supplemented simultaneously. The preparation can effectively reduce the incidence of hypoglycemia in newborn piglets, improve the disease resistance and immunity of the piglets, relieve weaning stress, improve the weaning survival rate and weaning weight, and simultaneously improve the nutritional supply of perinatal sows, reduce postpartum fatigue of the sows, improve milk quality and indirectly help the growth of the piglets.

The invention discloses a compound probiotic preparation for relieving heat stress of sows and application of the compound probiotic preparation, and belongs to the technical field of animal feed. The compound probiotic preparation comprises clostridium butyricum, bacillus subtilis, bacillus licheniformis and enterococcus faecalis, wherein the final concentration of the clostridium butyricum in the feed is 1 * 10 < 6 > CFU / g, and the final concentration of the bacillus subtilis, the final concentration of the bacillus licheniformis and the final concentration of the enterococcus faecalis in the feed is 5 * 10 < 6 > CFU / g. The preparation provided by the invention can effectively reduce the stress hormone level and oxidative damage index in the blood of the sow under the heat stress condition, improve the activity of antioxidant enzyme, improve the feed intake and lactation performance in the lactation period, and reduce the backfat loss. Meanwhile, through the vertical transfer effect of the sows and the piglets, the intestinal morphology of the offspring piglets is remarkably improved, the intestinal barrier function and the antioxidant capacity are enhanced, and the weaning weight and the average daily gain of the piglets are improved, so that the negative effects of heat stress on the reproductive performance of the sows and the growth of the piglets are comprehensively relieved. The preparation is green and safe, and has important application value.

The application provides a SNP molecular marker related to weaning weight of Yongdeng seven goats, a detection kit and application thereof, and belongs to the technical field of SNP molecular markers. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO. 1, the mutation site is the base at the 5343313th site on the 3rd chromosome of the international sheep genome Oar_v4.0 version, and the high weaning weight trait of Yongdeng seven goats can be identified by selecting the advantageous allele of the site through typing of the site. The application further provides a method for identifying the weaning weight trait of Yongdeng seven goats, screening or breeding Yongdeng seven goats with high weaning weight traits based on the SNP molecular marker. The method has high accuracy, fast detection speed, low cost, and easy result interpretation, and has important application value in realizing large-scale molecular precision breeding of Yongdeng seven goats.

This invention discloses the application of a SNP marker in the EGFR gene that affects weaning weight in sheep. The marker is located in the EGFR gene on sheep chromosome 19, specifically at the T / C base mutation at 367 bp of SEQ ID NO:1 in the sequence listing. Sheep with the TT and TC genotypes at this site have significantly higher weaning weights (at 60 days of age) than those with the CC genotype. Using Charolais sheep as the research subject, this invention utilizes PCR amplification of SNP variations in exon 14 of the sheep EGFR gene to analyze the effect of specific genotypes at this variation site on weaning weight in Charolais sheep. Based on this analysis, selective breeding of Charolais sheep can be conducted to improve weaning weight, providing marker resources for marker-assisted selection breeding of sheep growth traits.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to porcine reproductive traits and application thereof, the SNP molecular marker is located in a porcine REXO1 gene region, is located at a nucleic acid site at a 76689746th site of a chromosome 2 of an international porcine genome Sscrofa 11.1 version, and has C or T base polymorphism, and the nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1; wherein the reproductive performance of the CC type is superior to that of the CT type and the TT type; the breeding traits comprise the litter size, the live litter size, the birth weight and the weaning weight. The SNP molecular marker provided by the invention can be used for detection, breeding, cultivation and evaluation of sows with high reproductive performance.

The invention belongs to the technical field of Hu sheep molecular marker-assisted breeding, and particularly relates to application of molecular markers g.1151256C > T and g.1151437G > A in Hu sheep molecular marker-assisted breeding. A Hu sheep is taken as a test material, a specific primer pair is adopted to amplify an intron 6 segment of a CADM2 gene, three sites g.1151256C > T and g.1151437G > A are identified through PCR product sequencing and sequence comparison, a TT genotype in the g.1151256C > T is significantly related to a high weaning weight and a six-month-old weight, and an AA genotype in the g.1151437G > A is significantly related to the high weaning weight and the six-month-old weight. Accordingly, a CADM2 gene SNP molecular marker combination and a detection, interpretation and breeding process thereof are constructed, rapid identification of Hu sheep early growth dominant individuals is achieved, the method can be used for molecular marker-assisted selection of Hu sheep varieties, and the breeding efficiency and the genetic improvement level are improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to weaning weight of a Chinese Holstein calf and application of the SNP marker, and belongs to the technical field of dairy cow breeding molecular markers. The number of the SNP markers is 17, the SNP markers are respectively located in the range of 90845941-90887932bp of a cattle chromosome 7 and the range of 22916046-26644064bp of a cattle chromosome 14, and the SNP markers respectively form haplotypes which are annotated to candidate genes related to body weight, such as XKR4, PLAG1, CHCHD7, ARRDC3 and the like. Group verification proves that weaning weights of individuals with different genotypes of the 17 SNP markers are remarkably different. The SNP marker can be used for genetic improvement and marker-assisted selection of the weaning weight of calves, individuals carrying dominant alleles can be accurately screened, the breeding period is shortened, the economic benefits of dairy cow breeding are improved, and a new technical means and theoretical basis are provided for dairy cow molecular breeding.

The invention provides a method for constructing a Hu sheep lamb weaning weight prediction model, which comprises the following steps of: weighing and recording lamb birth weight when a set number of to-be-delivered Hu sheep ewes lamb, recording the number of lambs with the same birth and lamb sex, weighing and recording lamb weaning weight at the weaning day age of the lambs, and recording the lamb weaning weight at the weaning day age of the lambs; acquiring a data set including lamb birth weight, the number of lambs in the same fetus, lamb gender and lamb weaning weight, and dividing the data set into a training set and a verification set; performing multivariate stepwise regression analysis on the training set, establishing a prediction model of the weaning weight of the male lambs and the weaning weight of the female lambs, and checking the prediction model by using a root-mean-square error, a variable coefficient and a decision coefficient; and substituting the verification set into the prediction model, performing correlation analysis on a prediction result and an actual measurement result, and verifying the accuracy of the prediction model. According to the method, the construction process is relatively simple, the constructed prediction model is relatively high in reliability, and the weaning weight of the Hu sheep lambs can be accurately predicted according to the birth weight and the number of lambs of the same birth.

The application belongs to the technical field of marker-assisted breeding of Hu sheep, and particularly relates to application of molecular markers g.1151256C>T and g.1151437G>A in marker-assisted breeding of Hu sheep. With Hu sheep as test material, a specific primer is used to amplify a CADM2 gene intron 6 segment, and three sites of g.1151256C>T and g.1151437G>A are identified through PCR product sequencing and sequence alignment. In g.1151256C>T, the TT genotype is significantly related to higher weaning weight and six-month weight, and in g.1151437G>A, the AA genotype is significantly related to higher weaning weight and six-month weight. Accordingly, a CADM2 gene SNP molecular marker combination and its detection, interpretation and breeding process are constructed, early growth advantage individuals of Hu sheep are quickly identified, the molecular marker assisted selection of Hu sheep meat breeds can be used, and the breeding efficiency and genetic improvement level are improved.