The invention relates to application of butyrin lactate as a feed additive. The butyrin lactate conforms to the characteristics that the molecular formula is C13H22O8 and the molecular weight is 306.31. The application objects of the butyrin lactate as a feed additive include monogastric animals such as pigs, poultry and the like, ruminants such as cows, sheep and the like, herbivores such as horses, rabbits and the like and aquatic animals such as fishes, shrimps, crabs and the like. The application method comprises the following steps of: directly adding 0.05-0.5% of butyrin lactate into feed based on the total weight of the feed; or mixing the butyrin lactate with an adsorbent to obtain a premix; or mixing the butyrin lactate with other feed additives or feed raw materials to obtain a premix, a concentrate and the like to feed monogastric animals such as pigs, poultry and the like, ruminants such as cows, sheep and the like, herbivores such as horses, rabbits and the like and aquatic animals such as fishes, shrimps, crabs and the like. Through the invention, the butyrin lactate can be slowly decomposed in the gastrointestinal tracts of animals while releasing the characteristics of lactic acid and butyric acid, the pH value of the intestinal tract can be reduced, the intestinal microecology is improved, the growth of the intestinal mucosa can be promoted, the intestinal morphology is improved, and an effect of promoting the intestinal health of animals is realized so as to reduce diarrhea and improve the production performance of animals.