38 results about "Intestinal morphology" patented technology

The invention discloses a method for effectively relieving soybean antigen protein Glycinin in fish feed and application of the method. The invention discloses application of N-acetylcysteine as a functional feed additive capable of relieving carp enteritis induced by soybean antigen protein Glycinin. The feed disclosed by the invention comprises a soybean antigen protein Glycinin and N-acetylcysteine (NAC). According to the present invention, 1.5-6.0 g / kg of NAC is added to a feed containing 80 g / kg of Glycinin, such that the fish growth, the feed utilization rate, the digestion function, the protein metabolism function, the immune function, the oxidation resistance and the intestinal morphology can be significantly improved, and the soybean antigen protein Glycinin-induced growth inhibition and the intestinal injury can be effectively relieved; the functional fish feed additive provided by the invention is relatively low in cost and easy to popularize, and is beneficial to development of novel green antibiotic-replacing additives, improvement of the utilization rate of soybean protein sources, reduction of feed cost and promotion of healthy culture of fishes.

The invention discloses a lactobacillus mucus fermentation strain. The microbial preservation number of the lactobacillus mucus fermentation strain is CCTCC (China Center for Type Culture Collection) NO: M 20252262. The strain can inhibit enterotoxigenic escherichia coli, and can treat or slow down diarrhea, intestinal oxidative damage, intestinal inflammation damage, intestinal morphological damage, intestinal barrier function damage and intestinal flora disorder caused by the enterotoxigenic escherichia coli. The strain is a probiotic with a clinical application prospect.

The invention relates to the technical field of genomics and metabonomics, in particular to a method for analyzing intestinal health of crisp tilapia mossambica. The tilapia mossambica intestinal health judgment model is established by dynamically monitoring tilapia mossambica muscle texture and intestinal morphology through high time resolution, analyzing intestinal health three-dimensional indexes (tissue morphology-flora structure-host gene expression) and serum metabolism response after embrittlement succeeds, and combining cross-omics data association analysis. The change condition of the muscle texture can be known more timely, a more accurate basis is provided for adjustment of a feeding strategy, and the stability of the fish meat quality can be improved. The intestinal health is evaluated from three dimensions of histomorphology, flora structure and host gene expression, so that the health condition of the intestinal tract can be known more comprehensively, and more comprehensive reference is provided for optimization of a feeding method. The correlation between different omics data can be deeply analyzed, the physiological and metabolic mechanisms of the fish body are disclosed, and deeper theoretical support is provided for optimization of a feeding method.

The invention relates to the technical field of medical auxiliary instruments, in particular to a newborn radiography body position auxiliary fixing device which comprises a moving platform and a lifting platform arranged at the top of the moving platform, and further comprises a tightening mechanism arranged on the rear side of the top of the lifting platform and two body position clamping mechanisms arranged on the rear side of the top of the lifting platform. The problems that in the prior art, most devices are made of solid acrylic plates to be of a cylindrical closed structure, the overall weight is large, movement capacity is not achieved, operation is tedious and depends on an external assisting device, and the cost is low are solved. The problems that fixing, carrying, operation and the like of a child patient are limited, the examination process is blocked, diagnosis and treatment are delayed, binding force cannot be accurately controlled, the nursing difficulty is increased, the intestinal form is possibly interfered and image judgment is influenced due to increase of abdominal pressure, and the adaptability is poor are solved.

The invention discloses a preparation method and application of aminosalicylic acid xylan ester, and belongs to the technical field of glycan derivatives, the preparation method comprises the following steps: under the catalysis of diphenyl (2-hydroxyphenyl methyl) phosphine oxide, aminosalicylic acid and xylan are subjected to a reaction, and aminosalicylic acid modified xylan is prepared. In addition, the invention also provides an application of the aminosalicylic acid modified xylan in an animal breeding feed additive. According to the preparation method disclosed by the invention, the diphenyl (2-hydroxyphenylmethyl) phosphine oxide is utilized for catalysis, so that the aminosalicylic acid and the xylan are subjected to substitution reaction, complex functional group protection is not needed, and the yield and the purity are good; in the application of animal breeding feed, the modified product is added into the feed, so that the growth performance of bred animals such as chickens and the like can be remarkably improved, the intestinal morphology can be improved, and an efficient, simple and convenient preparation and application scheme is provided for feed additives.

This invention provides a method and application for improving the intestinal health of broilers using earthworm powder, belonging to the field of animal nutrition and feed technology. This invention solves the problems of poor growth performance, impaired intestinal health, and soybean meal substitution caused by nutritional deficiencies in broiler mixed meal diets. It includes the following steps: (1) raising earthworms in a substrate formed by mixing fermented chicken manure and Chinese medicine residue at a mass ratio of 2:3, and adding 2000 ppm sodium citrate to the substrate, controlling the substrate humidity at 50% to 55% and the temperature at 23 to 25°C; (2) preparing earthworm powder by freeze-drying the earthworms obtained in step (1) after disinfection; (3) replacing part of the soybean meal with 5.0% to 5.5% of the protein such as earthworm powder in the mixed meal broiler diet, and feeding the broilers. This invention significantly improves the growth performance, feed conversion ratio, immune function, intestinal digestive enzyme activity, intestinal morphology and barrier function of broilers, and optimizes the cecal microbiota, ultimately achieving efficient reduction and substitution of soybean meal and healthy and efficient broiler breeding.

This application discloses the application of creatine in the preparation of drugs or feed additives for the treatment or prevention of pathogenic diarrhea in pigs. The drugs are creatine or creatine as an active ingredient with added excipients to form oral liquids, granules, tablets, or powders. The dosage is 30–300 mg / kg of pig body weight; or it can be directly added to feed at a creatine content of 0.05%–0.5% of the total weight of the pig feed. The drugs or feed additives provided in this application are highly safe, have no toxic side effects, and can effectively prevent and treat pathogenic diarrhea caused by one or more pathogens, including porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, porcine rotavirus, and enterotoxigenic Escherichia coli. They improve the intestinal morphology and absorption function of sick pigs and reduce the morbidity and mortality of piglets.

The invention belongs to the technical field of animal nutrition and feed, discloses an application of a Fuzhuan tea extract in prevention of vomitoxin-caused piglet health injury, and particularly relates to an application of the Fuzhuan tea extract as a feed additive in prevention or improvement of piglet health injury. The feed additive can effectively reduce negative effects on weaned piglets caused by excessive vomitoxin in the feed, wherein the negative effects comprise growth performance reduction, diarrhea rate increase, intestinal morphology damage, intestinal cell proliferation inhibition, intestinal epithelial barrier injury, immune disorder, abnormal liver function and excessive oxidative stress and inflammatory response.

The invention discloses a feed additive for replacing antibiotics to treat chicken necrotic enteritis and application of the feed additive, and belongs to the technical field of feed. The effective component of the feed additive is the tannin extract from chestnut trees, and animal experiments prove that the tannin extract from chestnut trees can improve the production performance of broiler chickens with necrotic enteritis to a certain extent, improve the intestinal morphology and immune function of the broiler chickens with necrotic enteritis and improve the immunity of the broiler chickens with necrotic enteritis. Therefore, the chestnut tree tannin extract can replace antibiotics and has a good treatment effect on broiler intestinal inflammation caused by clostridium perfringens and coccidiosis.

The invention belongs to the field of animal models, and particularly relates to a zebrafish embryo model for 5-FU side effect research and a construction method and an evaluation method thereof. The construction method comprises the following steps: selecting zebrafish embryos for later use; and putting the zebrafish embryo into a culture hole, and then adding 5-FU for modeling treatment, thereby finishing the construction of the zebrafish embryo model for 5-FU side effect research. The 5-FU induced intestinal mucositis model is constructed by adopting a 3-5-day zebrafish embryo and analyzing information such as the survival rate, the travel trajectory, the intestinal morphology and ROS fluorescent living imaging of zebrafish, and the 5-FU induced intestinal mucositis model has the advantages of being simple in observation index, easy to observe, simple to operate, capable of saving operation time, convenient, rapid, high in efficiency and the like. The method has the characteristics of simple operation, high cost, cheaper mouse model, qualitative and quantitative properties and the like, and is suitable for high-throughput screening of 5-FU induced intestinal mucositis model drugs.

The invention relates to the field of livestock and poultry breeding, and discloses a laying hen feed additive based on fermented broussonetia papyrifera and application of the laying hen feed additive. The additive is prepared by taking paper mulberry leaves as a main material, matching with rice husks, wheat bran and other auxiliary materials, inoculating a microecological preparation containing lactobacillus plantarum, candida utilis and bacillus subtilis, and performing anaerobic fermentation. 4% of the additive is used for daily ration of the aged laying hens, so that the laying rate can be remarkably increased, the dirty egg rate can be reduced, and the egg weight, the eggshell strength and the yolk color can be improved; the mechanism is to improve the form of the intestinal tract, enhance the expression of tight junction protein, regulate immune balance, optimize the structure of cecum flora and enrich beneficial microorganisms; the broussonetia papyrifera leaf feed additive realizes efficient utilization of broussonetia papyrifera leaf resources, provides a safe and effective functional additive for prolonging the laying period of laying hens and improving the breeding benefits, and has a good application prospect.

The invention discloses a daily ration composition for improving growth performance and health of weaned piglets and application of the daily ration composition. The daily ration composition is formed by adding dihydroquercetin into basic daily ration serving as a carrier of weaned piglets, and the dihydroquercetin is added into the basic daily ration according to the proportion of 100 mg / kg. According to the invention, 100 mg / kg of dihydroquercetin is accurately added into the daily ration of the weaned piglets, so that the average daily gain of the piglets can be remarkably increased, the feed-gain ratio and the diarrhea rate can be reduced, meanwhile, the antioxidant enzyme activity and the immune globulin content of serum can be increased, the morphological structure of intestinal tracts can be improved, and no adverse effect is caused to the liver, the kidney, the spleen and other organs of the piglets.

The invention discloses application of a herba houttuyniae polysaccharide extract in a feed capable of improving intestinal health of piglets, the feed comprises the herba houttuyniae polysaccharide extract and a basic feed, and the application method of the herba houttuyniae polysaccharide extract comprises the following steps: adding the herba houttuyniae polysaccharide extract into the basic feed, and continuously feeding 21-28-day-old weaned piglets for 28 days, the intestinal flora structure of weaned piglets is adjusted through the houttuynia cordata polysaccharide extract, short-chain fatty acid secretion is promoted, and the intestinal morphology is improved, so that diarrhea prevention and intestinal health improvement are achieved; the herba houttuyniae polysaccharide extract is prepared from the following components in percentage by mass: 20 percent to 21.6 percent of herba houttuyniae polysaccharide, 5 percent to 10 percent of volatile oil, 25 percent to 35 percent of flavonoid, 10 percent to 15 percent of total amino acid, 3.1 percent to 6.1 percent of alkaloid, 3 percent to 10 percent of organic acid and 1.5 percent to 5.5 percent of sterol, and the herba houttuyniae polysaccharide is prepared from glucose, galactose, mannose, arabinose and xylose. The invention aims to improve the survival rate of early weaned piglets, shorten the breeding cycle of a farm and improve the reproductive performance.

The invention discloses a compound probiotic preparation for relieving heat stress of sows and application of the compound probiotic preparation, and belongs to the technical field of animal feed. The compound probiotic preparation comprises clostridium butyricum, bacillus subtilis, bacillus licheniformis and enterococcus faecalis, wherein the final concentration of the clostridium butyricum in the feed is 1 * 10 < 6 > CFU / g, and the final concentration of the bacillus subtilis, the final concentration of the bacillus licheniformis and the final concentration of the enterococcus faecalis in the feed is 5 * 10 < 6 > CFU / g. The preparation provided by the invention can effectively reduce the stress hormone level and oxidative damage index in the blood of the sow under the heat stress condition, improve the activity of antioxidant enzyme, improve the feed intake and lactation performance in the lactation period, and reduce the backfat loss. Meanwhile, through the vertical transfer effect of the sows and the piglets, the intestinal morphology of the offspring piglets is remarkably improved, the intestinal barrier function and the antioxidant capacity are enhanced, and the weaning weight and the average daily gain of the piglets are improved, so that the negative effects of heat stress on the reproductive performance of the sows and the growth of the piglets are comprehensively relieved. The preparation is green and safe, and has important application value.

The invention discloses a feed additive for improving oxidation resistance of weaned piglets and improving intestinal morphological integrity and application of the feed additive. It is found for the first time that when gamma-PGA of different doses is added into weaned piglet feed, the growth performance of weaned piglets can be remarkably improved, the apparent digestibility of nutrient substances in the feed is improved, the intestinal morphological integrity of small intestines is improved, and jejunum antioxidant enzyme activity and gene expression are promoted. The application of the feed additive is expected to improve the oxidation resistance of the weaned piglets and promote the intestinal morphological integrity of the piglets, and the feed additive has a wide application prospect in actual breeding production. In addition, the invention provides a new solution thought and method for improving animal growth performance and maintaining intestinal morphological integrity.

The invention discloses a preparation method of feed-grade pancreatin for promoting efficient growth of broiler chickens, and relates to the technical field of feed-grade pancreatin extraction.The method is characterized in that frozen pig pancreas is used as a raw material, and pancreatin is prepared through a series of procedures. The preparation method comprises the following specific steps: unfreezing, grinding into thick liquid, activating, degreasing, drying, crushing and the like to obtain the feed additive pancreatin. According to the method, the experiment process is optimized by taking the enzyme activity yield of trypsin in the extraction step as an index, the optimal condition of low-concentration ethanol in pancreatin extraction is explored, and process parameters which are easy to operate, high in enzyme activity and environmentally friendly are optimized. According to the method, the pancreatin extraction process is simplified, the environment-friendly effect is achieved only by using low-concentration ethanol in the extraction process, the pancreatin with high activity is prepared, the utilization rate of feed nutrients by broilers can be increased, the intestinal morphology of the broilers can be improved, the production performance of the broilers can be improved, and a basis and guidance are provided for production and application of low-protein daily ration.

The invention provides a friction coefficient measuring device for a capsule endoscope, which comprises a measuring bracket, an intestinal tract simulation piece and a first dynamometer, and is characterized in that the measuring bracket is provided with a testing plane; the intestinal tract simulation part comprises a telescopic actuator, an elastic framework and a flexible lining, the first end part of the elastic framework is mounted on the top surface of the test plane, the elastic framework is provided with a through accommodating hole along the length direction, the flexible lining is mounted on the inner wall of the accommodating hole, and the telescopic actuator is connected to the second end part of the elastic framework; the second end part of the elastic framework is driven to do telescopic motion along the length direction, so that various forms of straightness, bending and wriggling of the intestinal tract are simulated; the first dynamometer is connected with the capsule endoscope through a test line, pulls the capsule endoscope to move in the accommodating hole, and measures the pulling force of the capsule endoscope in the moving process, so that the friction force of the capsule endoscope in different intestinal forms is measured; the problem that the friction coefficient of the capsule endoscope in different intestinal forms is difficult to measure in the prior art is solved.

The present invention discloses an abdominal DR image processing method and system. The method includes the following steps: 1) Image preprocessing: performing ROI annotation, binarization processing, resampling processing, and boundary generation on the abdominal DR image to obtain a boundary binary image; 2) Statistical shape analysis: extracting the centerline, calculating the target width, and calculating the statistical shape of the boundary binary image obtained in step 1) to obtain a number of statistical shape parameters; 3) Image classification: performing image classification based on the number of statistical shape parameters obtained in step 2). The present invention proposes a method and system for analyzing the intestinal morphology of DR images based on statistical shape. Compared with the existing geometric morphology analysis scheme, the present invention has better stability for intestinal DR images with characteristics such as intestinal morphological changes, intestinal overlap phenomena, and patient position changes. The statistical shape parameters obtained by the present invention have better classification accuracy for image classification.

The present invention discloses a feed for largemouth bass added with gallnut tannic acid and ellagic acid. In the feed for largemouth bass, calculated by weight percentage, the feed for largemouth bass contains: 50% fish meal, 28.4% soybean meal, 6.6% tapioca starch, 8% soybean oil, 0.5% compound multivitamins, 0.5% compound multiminerals, 3.33125% carboxymethyl cellulose, 0.5% choline chloride, 2% calcium dihydrogen phosphate, 0.15% gallnut tannic acid and 0.01875% ellagic acid. By adding 0.15% gallnut tannic acid + 0.01875% ellagic acid to the basic feed for largemouth bass, it has no effect on the body composition of largemouth bass, but can improve the intestinal morphology, thereby effectively reducing the feed coefficient and increasing the growth performance. It well solves the problem of slow growth in the large-scale cultivation of largemouth bass.

The present invention relates to a method for constructing and identifying a transgenic fruit fly capable of inducing abnormal intestinal acidity, constructing a fruit fly transgenic vector pValium20-RagA RNAi, and obtaining RagA RNAi Transgenic fruit flies, through the double balancer fruit flies SP / SM6; MKRS / TM6 Crossing two generations to obtain a stable hereditary balancer gene UAS-RagA RNAi Transgenic fruit flies SP / SM6;UAS‑RagA RNAi / TM6 By crossing with NP1-GAL4 flies that drive gene expression in intestinal epithelial cells, we obtained a knockout strain of RagA in the intestine. NP1-GAL4;UAS-RagA RNAi Fruit flies. Phenotypic detection revealed that the intestinal morphology of the fruit flies was abnormal; the method of feeding bromophenol blue and cresyl purple revealed that the acidity in the intestine of the RagA knockout fruit flies was reduced; the method of detecting copper cells with immunofluorescence determined that the phenotype of abnormal gastrointestinal acidity in the fruit flies was related to a reduction in the number of cells that secrete hydrogen ions. These results confirmed that the intestinal acidity of the RagA knockout fruit flies was abnormal. The present invention can be used to screen drugs targeted at gastrointestinal acidity deficiency diseases more quickly and conveniently.

An improved agent and treatment method for a broad variety of diseases in both animals and humans are disclosed. The agent is an inventive treatment compound comprising a bacterial-based culture. The inventive treatment compound has a positive impact on the gut health of boilers from the earliest stage onward. These benefits are realized through several mechanisms, including improved gut morphology under disease stress. Healthier gut morphology and improved gut integrity result in improved nutrient uptake and growth benefits, both based on consumption of the disclosed inventive treatment compound. The downstream results are improvements in performance parameters related to gut health including altering gut microbes, feed conversion rates, and body weight gains among others. Use of the disclosed treatment compound has the further benefit of avoiding contamination during animal processing because of the reduction in the volume of pathogenic bacteria in the gut.

The present invention relates to the technical field of pea peptides, and specifically to pea peptides, a preparation method of modified pea peptides, and uses for improving gastrointestinal function. The amino acid sequence of the pea peptide is shown in SEQ ID NO.1. The preparation method of the modified pea peptide is prepared by reacting the pea peptide shown in SEQ ID NO.1 with monomethoxypolyethylene glycol-butyraldehyde. The uses include preparing intestinal antibacterial or antibacterial products, preparing products for improving intestinal immune function, preparing products for treating intestinal inflammation, preparing products for improving inflammatory intestinal morphology, preparing products for improving gastrointestinal hormone disorders, and preparing products for increasing intestinal short-chain fatty acid content.

The invention belongs to the technical field of animal husbandry, and particularly relates to application of ssc-miR-146a-5p in preparation of a product for promoting intestinal development of piglets. The nucleotide sequence of the ssc-miR-146a-5p is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention relates to a key ssc-miR-146a-5p for promoting proliferation of intestinal cells, which is screened from breast milk of sows with high and low piglet growth performance. The ssc-miR-146a-5p lentivirus overexpression vector is constructed and injected into suckling piglets in an abdominal cavity three-point injection mode, and it is found that overexpression of the ssc-miR-146a-5p can improve expression of intestinal cell proliferation genes and intestinal mucoprotein genes of the suckling piglets, promote intestinal development of the suckling piglets, improve the intestinal morphology and mucosal barrier and improve the growth of the suckling piglets. The growth performance of suckling piglets in the lactation period is improved. The ssc-miR-146a-5p provided by the invention can obviously improve the intestinal development and intestinal health of suckling piglets, and a feasible scheme is provided for solving the production problems of piglet diseases and death caused by slow intestinal development of the suckling piglets.

The application discloses application of caprylic capric glycerides in preparation of products for relieving or inhibiting intestinal cell iron death of piglets. It is found that the caprylic capric glycerides have a regulating effect on intestinal cell iron death of piglets, and feeding piglets with products containing the caprylic capric glycerides can relieve or inhibit intestinal epithelial cell iron death of piglets, protect the intestinal morphology and function of piglets, and relieve or inhibit intestinal cell iron death of piglets induced by Escherichia coli K88. Feeding piglets with feed products containing the caprylic capric glycerides can positively regulate the jejunal villus height and the ratio of the jejunal villus height to the crypt depth of piglets, the ultrastructure of the jejunal of piglets, the protein expression amount of the tight junction protein ZO-1 of the jejunal of piglets, the contents of 4-HNE, GPX4, MDA and GSH in the jejunal of piglets, and the protein expression amount of the iron death signal path related factors (FTH1, GPX4, SLC3A2 and TFR1) of the jejunal of piglets, and can positively and comprehensively protect the intestinal health of piglets.

The invention provides a rhizoma polygonati polysaccharide extract weaned piglet feed additive and application thereof.The feed additive comprises a rhizoma polygonati polysaccharide extract, and the rhizoma polygonati polysaccharide extract comprises, by mass, 36%-49.8% of rhizoma polygonati polysaccharide, 3%-12% of saponin, 2%-8% of flavone, 30%-45% of total amino acid and 0.2%-1% of alkaloid. Polygonatum sibiricum polysaccharide has various pharmacological activities such as anti-inflammation and bacteriostasis, immunity regulation, tumor resistance, oxidation resistance, blood sugar and blood fat reduction and the like, the polygonatum sibiricum polysaccharide extract is reasonably added into weaned piglet basic feed breeding, and the application dosage of the polygonatum sibiricum polysaccharide extract is 150-600 mg per kg of weaned piglet basic feed; the feed antibiotic can replace feed antibiotics, and can effectively reduce the diarrhea rate of weaned piglets, regulate the intestinal flora structure and improve the abdominal and intestinal morphology of the weaned piglets, so that the survival rate of the weaned piglets in the early stage is increased, the breeding cycle of a farm is shortened, and the reproductive performance is improved.

The invention discloses a bacillus coagulans strain. The microbial preservation number of the bacillus coagulans strain is CCTCC (China Center for Type Culture Collection) NO: M 20252261. The strain can treat or slow down intestinal oxidative damage, intestinal inflammation damage, intestinal morphological damage, intestinal barrier function damage and intestinal flora disorder caused by lipopolysaccharide, and can promote the growth of subjects. The strain is a probiotic with a clinical application prospect.

The present invention relates to the technical field of feed additives, and in particular to a feed additive composition for improving the intestinal health of livestock and poultry and its application. The feed additive composition provided by the present invention comprises the following components in parts by weight: 25-65 parts of ursolic acid, 1-10 parts of oligosaccharides, 1-15 parts of chlorogenic acid compounds, and 1-15 parts of sesquiterpenoid compounds. The feed additive composition can significantly increase the height of the villi in the small intestine of livestock and poultry, reduce the depth of the crypts, and significantly increase the villus-crypt ratio, thereby effectively improving the intestinal morphology. It can also effectively regulate the intestinal microbial population of livestock and poultry, improve intestinal health, promote the growth of livestock and poultry, and improve the immunity of livestock and poultry. It can be used as a safe, efficient, stable, and controllable green and environmentally friendly feed additive.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for preventing and treating chicken bacterial diarrhea, which is prepared from the following components in parts by weight: 20 to 50 parts of radix curcumae, 20 to 50 parts of cortex fraxini, 20 to 50 parts of honeysuckle stem, 40 to 80 parts of rheum officinale, 20 to 50 parts of fructus gardeniae, 10 to 30 parts of fructus chebulae, 10 to 30 parts of radix paeoniae alba and 10 to 30 parts of pericarpium zanthoxyli seed. The traditional Chinese medicine composition with different doses has a good clinical curative effect on chicken diarrhea caused by escherichia coli O78, and can relieve adverse effects of escherichia coli challenge on chicken by repairing tissue damage, regulating serum biochemical indexes and inflammatory factor content, improving intestinal morphology and reducing large intestine rod bacterium loading amount. According to the characteristics of multiple targets and multiple components of the compound traditional Chinese medicine, the effects of antibiosis, anti-inflammation, immunoregulation and the like are achieved, and meanwhile, the traditional Chinese medicine composition is nontoxic in clinical medication curative effect and safety evaluation and can be used for a long time.

The invention provides an application of enterococcus faecalis SZ07 in preparation of a trachinotus ovatus nervous necrosis virus resistant fish feed, the collection number of the enterococcus faecalis SZ07 is GDMCC No: 66159, and the collection center is Guangdong Microbial Culture Collection Center. Related experiments prove that the enterococcus faecalis SZ07 provided by the invention can effectively improve the intestinal morphology of the trachinotus ovatus, and more importantly, the enterococcus faecalis SZ07 can effectively regulate the immune response of a trachinotus ovatus body and improve the immunocompetence, antioxidant capacity and the like of the trachinotus ovatus, so that the antiviral capacity of the trachinotus ovatus is effectively improved, and the effect of preventing and treating the trachinotus ovatus is achieved. Particularly, the nervous necrosis virus resistance of the trachinotus ovatus is obviously improved. The enterococcus faecalis SZ07 provided by the invention can effectively prevent and control viral nervous necrosis of trachinotus ovatus, improve the survival rate, growth speed and disease resistance of trachinotus ovatus fries, and promote healthy development of trachinotus ovatus breeding industry.

The invention discloses a bacillus subtilis metagen as well as a preparation method and application thereof, and particularly relates to a bacillus subtilis metagen which is prepared from a strain with the preservation number of CGMCC No.37377. The strain is separated from eight-treasure fermented soya beans, and can tolerate 121 DEG C high-temperature and high-pressure inactivation treatment for 15 minutes and retain biological activity. The metabiotic composition is prepared by carrying out fermentation culture on the strain, collecting thalli and inactivating under the conditions, wherein the viable bacterium concentration before inactivation is 2.5 * 10 < 7 >-2.5 * 10 < 9 > CFU / mL. When being used as a feed additive to feed largemouth bass juvenile fish, the metagen can remarkably promote growth of the largemouth bass juvenile fish, improve serum lysozyme, complement C3, C4 and IgM level to enhance immunity, improve intestinal morphology and improve relative abundance of beneficial bacteria in intestinal tracts, thereby comprehensively improving intestinal health. The metagen is good in stability and high in safety, and has a good application prospect in the field of aquatic feed.