73 results about "Bass (fish)" patented technology

The invention discloses an underwater micropterus salmoides body length and mass measurement method, and relates to the technical field of aquaculture. Comprising the following steps: 1) target detection and key point detection: performing target detection and key point detection on underwater largemouth bass by using a YOLOv8 model to obtain key point coordinate information of a fish head and a fish tail; and 2) obtaining depth information: obtaining the depth information of the underwater image through a monocular depth estimation algorithm, and calculating the body length of the largemouth bass in combination with a key point detection result. And 3) mass estimation: based on the body length information, estimating the mass of the micropterus salmoides through a power function regression model. And 4) model deployment: deploying the model in a recirculating aquaculture monitoring system, and displaying and recording the body length and quality information of the micropterus salmoides in real time. According to the method, the body length and the body weight of the largemouth bass can be obtained in real time in a non-contact mode, the method is suitable for a factory-like circulating water culture mode, data support can be provided for culture management, and the practical value and the popularization prospect are high.

The invention belongs to the technical field of biology, and discloses application of wogonin in resisting siniperca chuatsi and frog iridovirus, the wogonin can effectively inhibit over-generation of ROS caused by MRV virus infection, and oxidative stress damage of host cells is relieved in a dose-dependent mode. The wogonin can be used for preventing and treating the siniperca chuatsi and frog iridovirus, the high expression state of a proinflammatory gene can be reduced, intestinal tissue damage induced by MRV infection can be relieved, proliferation of MRV in the micropterus salmoides is effectively inhibited, the MRV challenge survival rate of the micropterus salmoides is increased, a basis is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus from wogonin, and the wogonin has important application value. Meanwhile, the invention also discloses a medicine, a fish feed and a fish feed additive of the wogonin.

The invention discloses a targeted delivery system of a largemouth bass frog iridovirus oral vaccine and a preparation method of the targeted delivery system, and relates to the technical field of biological medicines.The method comprises the following steps that a starch solution and a largemouth bass frog iridovirus inactivated vaccine are stirred and mixed, then a paraffin emulsion is added, stirring and emulsifying are conducted, and a W / O emulsion is obtained; adding calcium chloride, stirring and mixing, centrifuging, and resuspending by using a PBS (Phosphate Buffer Solution) to obtain a microsphere resuspension solution; stirring and mixing with a quaternized chitosan solution, adding glutaraldehyde for crosslinking, centrifuging, and freeze-drying. The invention further discloses a targeted delivery system of the largemouth bass frog iridovirus oral vaccine prepared by the method. The vaccine is orally administered after being microencapsulated, so that the human cost is low, the stimulation to fish bodies is small, the problems of inconvenience in fish administration, low bioavailability of oral administration, instability in gastrointestinal tracts and the like are solved, and the vaccine has an excellent application prospect in the field of LMBV prevention and control.

The invention provides a full-indoor artificial breeding method for plectropomus leopardus, and belongs to the technical field of artificial breeding of marine fishes. The full-indoor artificial breeding method for the plectropomus leopardus comprises the steps of earlier-stage incubation of fertilized eggs, incubation in an indoor breeding pond, breeding in an indoor flowing water pond, breeding in an indoor circulating water pond and the like. By regulating and controlling illumination, the irritability of the fries is greatly reduced, the ecological conditions of the fries are effectively optimized, and the health condition and adaptability of the fries are improved. Meanwhile, different types and different total amounts of biological bait combinations are fed in the larva stage, the juvenile stage and the fish stage of the plectropomus leopardus, transition is made in each bait transfer period, enteritis caused by excessive intake of larvae and juveniles and too little intake caused by inadaptation of bait transfer are effectively reduced, and the problem that the survival rate is low in the bait transfer period can be effectively solved.

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention relates to the technical field of refrigeration, and provides a refrigeration device for perch processing, which comprises a mechanism, the inner upper part and the inner middle part of the refrigeration mechanism are fixedly connected with rotating frames, the lower ends of the rotating frames are rotatably connected with hanger mechanisms, and the inner middle part and the inner lower part of the refrigeration mechanism are slidably connected with water collecting boxes. The hanging frame mechanism comprises a cold air emitting assembly, six fish hanging assemblies are evenly distributed at the lower end of the cold air emitting assembly, and adjusting assemblies are fixedly connected to the middles of the fish hanging assemblies. Through fish hanging assemblies evenly distributed at the lower end of a cold air emitting assembly, when fishes with different weights are hung at the lower end of a hook, the angle of a rotating box is automatically adjusted through the weight of perches, then the perches with heavy mass and large size can be away from a sliding rod, and adjacent perches cannot adhere to each other; the freezing efficiency of the perches can be improved, and the situation that the perches are separated after being frozen and are damaged due to mutual adhesion of the perches is prevented.

The present invention relates to the use of alpha lipoic acid as feed additive for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and for warm water fish as for example carp, tilapia, catfish. More particular, this invention relates to the use of alpha lipoic acid for the improvement of the feed conversion ratio and / or daily weight gain in fish, for reducing mortality by regulating the micro flora of the gut and / or by protecting the animal against infections caused by pathogenic viruses.

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

This invention discloses an indoor breeding device for largemouth bass and its application. The device includes a parent selection unit and a spawning unit. The parent selection unit includes a parent rearing pond and a parent collection net cage, with the parent collection net cage located in the parent rearing pond near the inlet. The spawning unit includes a spawning pond and fish nests within the pond, wherein the fish nests are separated to form relatively independent breeding spaces within the pond, and a water flow system is installed above the fish nests. The advantages are: (1) This invention can effectively improve the indoor breeding efficiency of largemouth bass, from difficulty in spawning to a breeding rate of over 40%; (2) The application method utilizes the largemouth bass's head-up phenomenon to observe the gonadal development of the parent fish and uses this to screen for parent fish with mature gonads, greatly improving efficiency and without harming the fish fry; (3) When the parent fish spawn, the device adaptively adjusts the water flow in the pond according to the first collection of fertilized eggs to ensure the breeding rate of the parent fish.

The invention discloses a facility circulating water separation grading culture method for micropterus salmoides. The method comprises the following steps: identifying the sex of the micropterus salmoides according to specific primers, and separating the sex of the micropterus salmoides from the sex of the micropterus salmoides; according to different growth speeds of male and female micropterus salmoides and individual micropterus salmoides, a three-stage breeding mode is adopted, and sequencing batch type grading breeding is carried out; special compound feed for male and female micropterus salmoides is fed; a corresponding feeding strategy is adopted according to the conditions of weight, water temperature, water quality and the like; the organism health of micropterus salmoides is enhanced by adopting a composite technical means, the microenvironment stability of a culture water body is regulated and controlled, and stress damage caused by environmental fluctuation and pathogenic organisms is reduced. According to the method for culturing the micropterus salmoides in a classified manner through facility circulating water separation, the single-pond yield of the micropterus salmoides cultured in the facility circulating water can be effectively improved, meanwhile, the culture cost is reduced, the culture benefit is improved, off-peak marketing is achieved, and efficient and sustainable development of facility fishery is maintained.

The invention provides application of rhodopseudomonas palustris in improvement of oxidation resistance, meat quality and liver and intestine health of largemouth bass, and belongs to the technical field of aquaculture. The rhodopseudomonas palustris or the feed containing the rhodopseudomonas palustris is applied to improving the meat quality of the largemouth bass and / or improving the fish meat taste of the largemouth bass. Meanwhile, the invention also provides application of the rhodopseudomonas palustris in preparation of a product for improving the oxidation resistance of the largemouth bass or maintaining the health of the liver and / or the intestinal tract of the largemouth bass. The Rhodopseudomonas palustris not only can significantly improve the muscle quality of Micropterus salmoides, but also can improve serum and liver oxidation resistance, improve liver health, improve intestinal digestive enzyme activity, regulate intestinal flora and improve intestinal health, and the Rhodopseudomonas palustris has the best effect when being added into Micropterus salmoides feed in the form of living bacteria.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

1. The name of the design product: lure (deep bass). 2. The use of the design product: fish bait for fishing. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

The present invention discloses a low-protein feed containing a largemouth bass polypeptide and its application. The amino acid sequence of the largemouth bass polypeptide is shown in SEQ ID NO. 1. By adding a largemouth bass polypeptide (MSNP) to the feed and combining it with fermented soybean meal, the present invention improves the largemouth bass's ability to digest the feed and absorb and utilize nutrients, promotes the growth and weight gain of the largemouth bass, reduces the total protein content of the feed and the amount of fish meal added, helps save feed costs, improves aquaculture efficiency, and reduces pollution pressure on aquaculture water bodies. The invention has broad application value in the largemouth bass aquaculture industry.

The invention discloses oxygenation equipment for improving the bearing capacity of perch land-based culture, and relates to the technical field of oxygen supply of culture water, in particular to oxygenation equipment for improving the bearing capacity of perch land-based culture, which comprises a water pump, the left side of the water pump is connected with a gas-liquid fusion pipe through a pipeline, and the gas-liquid fusion pipe is mounted outside a pond; a flow divider is fixedly connected to the top end of the gas-liquid fusion pipe, the upper end of the flow divider is connected with a water pump pipeline, a first high-oxygen water outlet pipe is fixedly installed at the lower end of the gas-liquid fusion pipe, and a left side pipeline of the first high-oxygen water outlet pipe is connected with a second high-oxygen water outlet pipe located in the pool. According to the oxygenation equipment for aquaculture, through cooperation of a water pump, a flow divider, a gas-liquid fusion pipe and a second high-oxygen water outlet pipe, the effect that gas and liquid are fully mixed in the structure space under low pressure is achieved through the arrangement of the gas-liquid fusion pipe, the mixed gas passes through a second high-oxygen water outlet pipe pool and then is tangentially jetted, a water body is pushed to rotate, and the water body is purified. Meanwhile, high-oxygen and low-oxygen water bodies are promoted to be fused, the oxygenation complexity is simplified, and redundant cumbersome facilities and equipment in the fishpond are replaced.

The invention discloses a sex molecular marker primer group, a preparation method and application thereof and a breeding method of all-male largemouth bass, and relates to the field of all-male largemouth bass breeding, the sex molecular marker primer group comprises a primer pair I or a primer pair II for screening genetic male largemouth bass, and a primer pair III for screening super-male largemouth bass; in the primer pair I, the sequence of a forward primer F1 is as shown in SEQ ID No: 1, and the sequence of a reverse primer R1 is as shown in SEQ ID No: 2; in the primer pair II, the sequence of a forward primer F2 is as shown in SEQ ID No: 3, and the sequence of a reverse primer R2 is as shown in SEQ ID No: 4; in the third primer pair, the sequence of a forward primer F3 is shown as SEQ ID No: 5, and the sequence of a reverse primer R2 is shown as SEQ ID No: 6. The primer group is designed according to male specific fragments, PCR detection is carried out on the basis of specific primers, super-male micropterus salmoides can be obtained through screening, and then all-male micropterus salmoides groups are obtained through culture.

The present application belongs to the field of feed, and discloses a feed attractant for low fish meal feed for carnivorous fish and application. The feed attractant comprises 7-9 parts of clam extract, 7-9 parts of squid by-product extract, 5-7 parts of amino acid, 8-12 parts of extract liquid of traditional Chinese medicine herb with a concentration of 1g / mg, and 4-6 parts of aquatic animal offal fermentation product. The feed attractant can improve the feed attraction and palatability of low fish meal feed, significantly improve the feeding rate of large mouth bass, promote the growth of large mouth bass, and reduce the feed coefficient. The feed attractant has good feed attraction effect on large mouth bass of each age stage. The present application also discloses low fish meal feed for large mouth bass and a preparation process. In the case of replacing fish meal with a large amount of non-animal protein, the appetite of large mouth bass is not reduced, feed waste is reduced, and the demand for large-scale breeding of large mouth bass is met.

The utility model discloses a big mouth black bass sulphate salinity adaptation domestication device belongs to the domestication technical field of cultivation, this big mouth black bass sulphate salinity adaptation domestication device, and the domestication mechanism includes domestication pool subassembly, and domestication pool subassembly is installed in the top of support subassembly, and domestication pool subassembly includes pool body, and the bottom intercommunication of pool body is provided with sulphate pool, and the inner bottom intercommunication of pool body is provided with trachea, the outside intercommunication of domestication pool subassembly is provided with water circulation subassembly, and water circulation subassembly includes overflow pool, and overflow pool is provided with the top intercommunication of pool body, through the mode of air blowing bubble of air outlet pipe, can after sulphate solution enters pool body through delivery pipe, intercommunication pipe, output pipe, the solution is diffused in pool body fast, avoids local concentration difference, and need not additional mechanical stirring subassembly, reduces water body disturbance (reduces fish stress risk) again, realizes " mixing - oxygenation " dual function.

The invention relates to the technical field of aquaculture, in particular to a culture method and application of gill explants of micropterus salmoides. According to the technical scheme, the culture method of the gill explant of the largemouth bass comprises the following steps: culturing the gill explant of the largemouth bass by using an explant culture solution; the explant culture solution is formed by adding fetal calf serum, fish serum, three antibodies, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In cooperation with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be reserved, and the metabolic activity and functional integrity of the gill tissue can be maintained. According to the technical scheme, the technical problem that an ideal research model capable of maintaining the structural integrity of the gill tissue and accurately reflecting the functional characteristics of the gill tissue cannot be provided in the prior art can be solved. By means of the culture method, research models of ammonia nitrogen stress and the like can be efficiently constructed, and technical support is provided for micropterus salmoides gill tissue physiological mechanism research, disease prevention and control, environmental risk assessment and the like.

The application relates to the technical field of aquaculture, and discloses a large-mouth black bass hatching method based on a factory-like circulating water aquaculture system, which comprises the following steps: step one, obtaining water temperature data of each region in a breeding pond where parent fish are put through multiple types of temperature sensors and presetting a natural light simulation period; step two, analyzing the activity of the parent fish in the natural light simulation period, obtaining the activity of the parent fish in the light period, obtaining the breeding behavior of the parent fish through the activity of the parent fish in the light period, and estimating the breeding proportion of the parent fish in the breeding pond according to the breeding behavior of the parent fish. The water temperature sensors are used for collecting the water temperature of each region in the breeding pond in real time, a light simulation period that is consistent with the natural rhythm is preset, the breeding behavior of the parent fish is identified and verified, and the influence weight of the water temperature on the breeding behavior and the influence of the light intensity and the activity of the parent fish are determined according to the water temperature deviation analysis.

The invention provides a cyclic peak shifting marketing method for micropterus salmoides, and belongs to the technical field of aquaculture. In the factory-like circulating aquaculture system in the greenhouse, three elements of multi-batch seedling casting, dynamic pond division and facility comprehensive utilization are integrated into an organic circulating production system, a brand-new production rhythm is constructed, and two long-term technical problems of accurate peak shifting and efficient facility utilization are solved. The method is clear in step and high in operability, can be directly popularized and applied in a greenhouse with basic temperature control and circulating water facilities, finally achieves the core commercial purpose that fishes appear on the market continuously in the high-price period from April to August, and is extremely remarkable in economic benefit.

The present application relates to a new application of radix cirsii japonici extract in resisting largemouth bass iridovirus. The present application finds that the radix cirsii japonici extract has a significant effect in resisting largemouth bass iridovirus, can significantly reduce the copy number of largemouth bass iridovirus, inhibit the expression of largemouth bass iridovirus MCP protein, improve the phagocytic ability of largemouth bass kidney leukocytes, and effectively improve the survival rate of fish infected with largemouth bass iridovirus, and has good biological safety. Therefore, the radix cirsii japonici extract can be used for preparing products such as drugs, fish feed or fish feed additives resisting largemouth bass iridovirus, and provides an effective prevention and control means for largemouth bass and other fish breeding.

The invention discloses an application of a herba cepbalanoplosis segeti extract in preparation of an inhibitor for fish-derived aeromonas veronii. The herba cepbalanoplosis segeti extract is obtained by extracting a herba cepbalanoplosis segeti sample with water or an organic solvent. The herba cepbalanoplosis segeti extract prepared by the invention has a relatively good inhibition effect on largemouth bass source aeromonas veronii, grass carp source aeromonas veronii and tilapia source aeromonas veronii, and has the characteristics of high efficiency, safety and no toxic or side effect. The herba cepbalanoplosis segeti extract disclosed by the invention is prepared into aquaculture feed, so that bacterial drug resistance caused by excessive use of antibiotics for preventing and treating fish source aeromonas veronii in aquaculture can be avoided, and healthy, stable and sustainable development of aquaculture industry is facilitated.

The present invention provides a selective breeding method and a cultivation method for largemouth bass with a slender body type, belonging to the technical field of aquaculture. In order to selectively breed and cultivate largemouth bass with a high proportion of slender body types, the present invention provides a selective breeding method and a cultivation method for largemouth bass with a slender body type. Through continuous selection with the slender body type standard and body weight as indicators, a largemouth bass population with a significantly increased proportion of largemouth bass with a slender body type is obtained after 4 years of screening. At the same time, by feeding a feed containing dandelion, purslane, and wild chrysanthemum in combination with water quality treatment, adult fish groups with uniform body types and a large proportion of slender body types are cultivated, greatly increasing the economic benefits of farmers and being suitable for large-scale popularization and application.

The invention discloses a largemouth bass feed taking hermetia illucens as a raw material. Comprising the following raw materials in parts by weight: 150-300 parts of hermetia illucens larva powder, 100-200 parts of fish meal, 50-100 parts of soybean meal, 30-60 parts of wheat flour, 30-50 parts of corn flour, 10-30 parts of casein, 20-40 parts of enzymolysis fish soluble, 10-20 parts of yeast powder, 10-20 parts of fish oil, 20-30 parts of soybean lecithin, 8-15 parts of monocalcium phosphate, 3-8 parts of a vitamin premix, 5-12 parts of a mineral premix, 2-5 parts of choline and 0.2-1.0 part of an antioxidant. 20-40 parts of pregelatinized starch and 3-8 parts of sodium alginate. The feed is reasonable in design, and the formula comprises various raw materials, so that balanced nutrition is ensured.

The invention provides application of a compound based on a lauric acid structure in preparation of drugs and / or fish feed for resisting iridovirus of micropterus salmoides. The structural formula of the compound is shown in the specification. Wherein R is H, or R contains hydroxyl. The compound is lauric acid or certain specific derivatives based on lauric acid, and related experiments prove that the compound can effectively prevent and / or treat the largemouth bass iridovirus (LMBV), is a novel medicine for preventing and controlling the largemouth bass iridovirus, and is relatively low in biotoxicity and good in clinical application prospect. And obvious toxicity or side reaction influence on fishes easily infected with LMBV, such as micropterus salmoides, is avoided.

The invention discloses application of rhein in preparation of a medicine for resisting iridovirus of micropterus salmoides. It is found and proved for the first time that rhein can be used as a fish feed additive, has good in-vivo safety, can be used as a drug for resisting LMBV infection of largemouth bass after being fed, has a good effect on resisting LMBV infection, can improve the survival rate of largemouth bass infected with LMBV, inhibits expression of virus in target tissue, and has good application prospects. Virus-induced fish target tissue structure damage is relieved, and the death rate caused by virus infection is reduced. Besides, as a disease-resistant immunopotentiator, the compound has the function of maintaining intestinal mucosa barrier, can maintain intestinal health of largemouth bass, can be better applied to prevention and control of largemouth bass iridovirus diseases, and can be used for preparing antiviral products.

The application discloses a compound feed for high-salt-alkaline water aquaculture of large-mouth black bass and a preparation method thereof. The raw material composition comprises 42-46 parts of fish meal, 6-8 parts of pork meal, 5-7 parts of casein, 13-17 parts of soybean meal, 10-14 parts of wheat flour, 4.5-8.5 parts of fish oil, 0.5-2 parts of sodium carboxymethyl cellulose, 1-3 parts of Ca(H2PO4)2, 6-8 parts of microcrystalline cellulose, 0.5-2 parts of premix, and 0.1-1 part of choline chloride. When the fat content is 10wt%-12wt%, the compound feed can improve the lipid metabolism of serum and liver of the large-mouth black bass, protect the liver, improve the immune and antioxidant enzyme activity, reduce the tissue damage induced under high-salt-alkaline water aquaculture, improve the intestinal flora disorder of the large-mouth black bass by increasing the number of intestinal probiotics such as phocaebacterium, and further improve the growth performance, immunity and antioxidant stress capacity of the large-mouth black bass under high-salt-alkaline stress.