33 results about "Bass (fish)" patented technology

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention relates to the technical field of refrigeration, and provides a refrigeration device for perch processing, which comprises a mechanism, the inner upper part and the inner middle part of the refrigeration mechanism are fixedly connected with rotating frames, the lower ends of the rotating frames are rotatably connected with hanger mechanisms, and the inner middle part and the inner lower part of the refrigeration mechanism are slidably connected with water collecting boxes. The hanging frame mechanism comprises a cold air emitting assembly, six fish hanging assemblies are evenly distributed at the lower end of the cold air emitting assembly, and adjusting assemblies are fixedly connected to the middles of the fish hanging assemblies. Through fish hanging assemblies evenly distributed at the lower end of a cold air emitting assembly, when fishes with different weights are hung at the lower end of a hook, the angle of a rotating box is automatically adjusted through the weight of perches, then the perches with heavy mass and large size can be away from a sliding rod, and adjacent perches cannot adhere to each other; the freezing efficiency of the perches can be improved, and the situation that the perches are separated after being frozen and are damaged due to mutual adhesion of the perches is prevented.

The invention relates to a sex-related SNP (Single Nucleotide Polymorphism) molecular marker of lateolabrax japonicus as well as a detection reagent and application thereof, and belongs to the technical field of fish molecular breeding. The invention provides a reagent for detecting SNP (Single Nucleotide Polymorphism) sites in a lateolabrax japonicus genome. The reagent is used for detecting genotypes of an SNP site 1, an SNP site 2 and an SNP site 3. The SNP site 1 is located at a 4806373 bp position of a chromosome 4 of a lateolabrax japonicus genome, the SNP site 2 is located at a 16421908 bp position of a chromosome 5 of the lateolabrax japonicus genome, and the SNP site 3 is located at a 7808123 bp position of a chromosome 18 of the lateolabrax japonicus genome. A group of lateolabrax japonicus sex-related SNP molecular markers are screened and used for molecular marker-assisted sex identification and early breeding of lateolabrax japonicus, and a technical tool and a reference basis are provided for rapid screening and cultivation of unisexual lateolabrax japonicus populations with better growth traits.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The invention provides application of rhodopseudomonas palustris in improvement of oxidation resistance, meat quality and liver and intestine health of largemouth bass, and belongs to the technical field of aquaculture. The rhodopseudomonas palustris or the feed containing the rhodopseudomonas palustris is applied to improving the meat quality of the largemouth bass and / or improving the fish meat taste of the largemouth bass. Meanwhile, the invention also provides application of the rhodopseudomonas palustris in preparation of a product for improving the oxidation resistance of the largemouth bass or maintaining the health of the liver and / or the intestinal tract of the largemouth bass. The Rhodopseudomonas palustris not only can significantly improve the muscle quality of Micropterus salmoides, but also can improve serum and liver oxidation resistance, improve liver health, improve intestinal digestive enzyme activity, regulate intestinal flora and improve intestinal health, and the Rhodopseudomonas palustris has the best effect when being added into Micropterus salmoides feed in the form of living bacteria.

The invention discloses a sex molecular marker primer group, a preparation method and application thereof and a breeding method of all-male largemouth bass, and relates to the field of all-male largemouth bass breeding, the sex molecular marker primer group comprises a primer pair I or a primer pair II for screening genetic male largemouth bass, and a primer pair III for screening super-male largemouth bass; in the primer pair I, the sequence of a forward primer F1 is as shown in SEQ ID No: 1, and the sequence of a reverse primer R1 is as shown in SEQ ID No: 2; in the primer pair II, the sequence of a forward primer F2 is as shown in SEQ ID No: 3, and the sequence of a reverse primer R2 is as shown in SEQ ID No: 4; in the third primer pair, the sequence of a forward primer F3 is shown as SEQ ID No: 5, and the sequence of a reverse primer R2 is shown as SEQ ID No: 6. The primer group is designed according to male specific fragments, PCR detection is carried out on the basis of specific primers, super-male micropterus salmoides can be obtained through screening, and then all-male micropterus salmoides groups are obtained through culture.

The present application belongs to the field of feed, and discloses a feed attractant for low fish meal feed for carnivorous fish and application. The feed attractant comprises 7-9 parts of clam extract, 7-9 parts of squid by-product extract, 5-7 parts of amino acid, 8-12 parts of extract liquid of traditional Chinese medicine herb with a concentration of 1g / mg, and 4-6 parts of aquatic animal offal fermentation product. The feed attractant can improve the feed attraction and palatability of low fish meal feed, significantly improve the feeding rate of large mouth bass, promote the growth of large mouth bass, and reduce the feed coefficient. The feed attractant has good feed attraction effect on large mouth bass of each age stage. The present application also discloses low fish meal feed for large mouth bass and a preparation process. In the case of replacing fish meal with a large amount of non-animal protein, the appetite of large mouth bass is not reduced, feed waste is reduced, and the demand for large-scale breeding of large mouth bass is met.

The application relates to the technical field of aquaculture, and discloses a large-mouth black bass hatching method based on a factory-like circulating water aquaculture system, which comprises the following steps: step one, obtaining water temperature data of each region in a breeding pond where parent fish are put through multiple types of temperature sensors and presetting a natural light simulation period; step two, analyzing the activity of the parent fish in the natural light simulation period, obtaining the activity of the parent fish in the light period, obtaining the breeding behavior of the parent fish through the activity of the parent fish in the light period, and estimating the breeding proportion of the parent fish in the breeding pond according to the breeding behavior of the parent fish. The water temperature sensors are used for collecting the water temperature of each region in the breeding pond in real time, a light simulation period that is consistent with the natural rhythm is preset, the breeding behavior of the parent fish is identified and verified, and the influence weight of the water temperature on the breeding behavior and the influence of the light intensity and the activity of the parent fish are determined according to the water temperature deviation analysis.

The invention provides a cyclic peak shifting marketing method for micropterus salmoides, and belongs to the technical field of aquaculture. In the factory-like circulating aquaculture system in the greenhouse, three elements of multi-batch seedling casting, dynamic pond division and facility comprehensive utilization are integrated into an organic circulating production system, a brand-new production rhythm is constructed, and two long-term technical problems of accurate peak shifting and efficient facility utilization are solved. The method is clear in step and high in operability, can be directly popularized and applied in a greenhouse with basic temperature control and circulating water facilities, finally achieves the core commercial purpose that fishes appear on the market continuously in the high-price period from April to August, and is extremely remarkable in economic benefit.

The present application relates to a new application of radix cirsii japonici extract in resisting largemouth bass iridovirus. The present application finds that the radix cirsii japonici extract has a significant effect in resisting largemouth bass iridovirus, can significantly reduce the copy number of largemouth bass iridovirus, inhibit the expression of largemouth bass iridovirus MCP protein, improve the phagocytic ability of largemouth bass kidney leukocytes, and effectively improve the survival rate of fish infected with largemouth bass iridovirus, and has good biological safety. Therefore, the radix cirsii japonici extract can be used for preparing products such as drugs, fish feed or fish feed additives resisting largemouth bass iridovirus, and provides an effective prevention and control means for largemouth bass and other fish breeding.

The invention discloses an application of a herba cepbalanoplosis segeti extract in preparation of an inhibitor for fish-derived aeromonas veronii. The herba cepbalanoplosis segeti extract is obtained by extracting a herba cepbalanoplosis segeti sample with water or an organic solvent. The herba cepbalanoplosis segeti extract prepared by the invention has a relatively good inhibition effect on largemouth bass source aeromonas veronii, grass carp source aeromonas veronii and tilapia source aeromonas veronii, and has the characteristics of high efficiency, safety and no toxic or side effect. The herba cepbalanoplosis segeti extract disclosed by the invention is prepared into aquaculture feed, so that bacterial drug resistance caused by excessive use of antibiotics for preventing and treating fish source aeromonas veronii in aquaculture can be avoided, and healthy, stable and sustainable development of aquaculture industry is facilitated.

The application discloses a compound feed for high-salt-alkaline water aquaculture of large-mouth black bass and a preparation method thereof. The raw material composition comprises 42-46 parts of fish meal, 6-8 parts of pork meal, 5-7 parts of casein, 13-17 parts of soybean meal, 10-14 parts of wheat flour, 4.5-8.5 parts of fish oil, 0.5-2 parts of sodium carboxymethyl cellulose, 1-3 parts of Ca(H2PO4)2, 6-8 parts of microcrystalline cellulose, 0.5-2 parts of premix, and 0.1-1 part of choline chloride. When the fat content is 10wt%-12wt%, the compound feed can improve the lipid metabolism of serum and liver of the large-mouth black bass, protect the liver, improve the immune and antioxidant enzyme activity, reduce the tissue damage induced under high-salt-alkaline water aquaculture, improve the intestinal flora disorder of the large-mouth black bass by increasing the number of intestinal probiotics such as phocaebacterium, and further improve the growth performance, immunity and antioxidant stress capacity of the large-mouth black bass under high-salt-alkaline stress.

1. The name of the design product: fishing rod (tomato bass fishing rod). 2. The use of the design product: mainly used for fishing rod for fishing tomato bass. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design. 6. The design product is an elongated article, and the length is omitted.

The invention discloses a composite single-cell protein feed for improving anti-infection immunity of micropterus salmoides and application of the composite single-cell protein feed, and belongs to the technical field of feed preparation. The feed comprises composite single-cell protein for replacing 40-100% of fish meal, and the composite single-cell protein comprises chlorella and clostridium ethanol protein in a mass ratio of 1: (4-5). The compound single-cell protein is adopted to replace part of fish meal in the feed, so that the cost of the feed can be remarkably reduced, the anti-infection immunity of the micropterus salmoides to aeromonas hydrophila can be improved, expression down-regulation of a pro-apoptosis gene casp8 and an antigen presentation related gene mhci in the micropterus salmoides can be promoted, and the apoptosis resistance of the micropterus salmoides can be improved. The expression up-regulation of the anti-apoptosis gene bcl2l1 and the anti-ferroptosis gene gpx4a is promoted. In addition, the liver metabolism function of the micropterus salmoides can be improved, and lipid metabolism is promoted.

The invention belongs to the technical field of aquaculture, and discloses a composition targeting a leptin gene of micropterus salmoides and capable of improving the feed utilization rate as well as a preparation method and application of the composition. The composition is prepared from the following components in parts by weight: 1 to 30 parts of wax gourd polysaccharide, 5 to 35 parts of gamma-aminobutyric acid and 1 to 20 parts of siRNA. Through complementary preparation of wax gourd polysaccharide, gamma-aminobutyric acid and siRNA, triple effects of gene silencing, appetite promotion and metabolism enhancement are realized. According to the invention, a wax gourd polysaccharide water extraction and alcohol precipitation process, a siRNA chemical synthesis process and a double-phase embedding-gradient composite process are adopted, so that the stability and bioavailability of active ingredients are improved. By down-regulating leptin expression, appetite and feed utilization rate of the micropterus salmoides fed with high-sugar feed are enhanced, blood sugar and blood fat levels are reduced, growth of fish bodies is promoted, and the micropterus salmoides culture medium can be applied to the field of micropterus salmoides culture.

This invention relates to the field of fish feed technology, specifically to a precise nutritional formulation method for low-fishmeal largemouth bass compound feed. It includes the following steps: S1, extruding and puffing the low-fishmeal base raw material, controlling a specific extrusion temperature and instantaneous pressure drop at the discharge port to obtain an extruded granular skeleton with a connected microporous structure; S2, adding an aqueous phase containing water-soluble gut-protecting factors to an oil phase containing a fat-soluble attractant and a low HLB value emulsifier, and preparing a W / O type microemulsion through high-speed homogenization and encapsulation; S3, placing the puffed skeleton in a vacuum spraying machine under negative pressure, spraying the microemulsion, and then gradually depressurizing in a stepwise manner to achieve deep anchoring of the microemulsion. This invention utilizes the fat-soluble oil phase to form a natural waterproof barrier to prevent water loss, and competitive adsorption under the action of high concentrations of bile acids in the largemouth bass intestine to achieve targeted demulsification and release, significantly improving hepatointestinal health and survival rate.

The invention discloses a method for producing all-male largemouth micropterus salmoides based on soaking induction of sex reversal of largemouth micropterus salmoides, and particularly relates to the field of artificial propagation of all-male largemouth micropterus salmoides, the method comprises the following steps: S01, taking fairy shrimps as initial feed when largemouth micropterus salmoides fries obtained by incubation swim flatly; s02, transferring the largemouth micropterus salmoides fry into a water body in which a sexual reversal induction reagent is dissolved, keeping feeding three times every day during the period, and soaking and breeding the fry for 60 days; and S03, after soaking and culturing for 60 days, transferring the largemouth bass fry into a conventional culture water body for culturing, and after sexual maturity of the largemouth bass fry, obtaining a male population capable of generating seminal fluid, namely the all-male largemouth bass. According to the method, largemouth bass fries are soaked in a sex reversal induction reagent with a specific concentration, sex reversal fishes with a high survival rate and a low malformation rate can be directly obtained, and then large-scale cultivation of all-male largemouth bass is achieved.

The invention discloses a free amino acid multi-index identification system for bighead carp identity tracing and application of the free amino acid multi-index identification system. The free amino acid multiple indexes comprise at least three of aspartic acid, glycine, alpha-amino n-butyric acid, methionine and 3-methylhistidine. The identification system comprises a method for distinguishing wild and cultured bighead carps, and the method comprises the following steps: measuring the content of amino acids in a specific combination in a sample to be detected, inputting the content into a preset logistic regression model to calculate a wild probability value, and comparing the wild probability value with a distinguishing threshold value optimized and determined through an ROC curve, so as to judge a source. According to the technical scheme, the specific biomarker for bighead carp source discrimination is defined for the first time, and the problem that a traditional experience discrimination method is high in subjectivity is solved. By constructing the quantitative model, rapid, objective and high-precision discrimination is realized, and the discrimination accuracy of the optimal combination model can reach 97.8%. The method provides reliable technical support for identifying illegal fished catches in fishery administration law enforcement in the Yangtze River basin, and has important significance for protecting wild bighead carp resources.

The application discloses a large-mouth bass fry overwintering domestication and engineering circulating water breeding method and relates to the technical field of aquaculture. The breeding method comprises the following steps: overwintering domestication of fish fry, construction of an ecological breeding system and breeding of the domesticated fish fry by using the system; the overwintering domestication of the fish fry is to domesticate the fish fry with a body length of 0.9-1.2 mm to large-size fish fry with a body length of 6-8 cm so that the fish fry can adapt to artificial compound feed; the domestication time is from November to December of each year to March of the next year; and the domestication is divided into first-stage domestication and second-stage domestication. The overwintering domestication of the fish fry is achieved by strictly adjusting the feeding mode and reducing the stress damage, the problem of low survival rate caused by too early domestication can be solved, the fish fry can smoothly overwinter, large-size fish fry that is well domesticated can be obtained in advance, the well-domesticated fish fry is bred in the breeding system, the intensive, intelligent, green and ecological breeding effect can be achieved, the fish fry can be put on the market in advance and a higher market price can be obtained.

The invention discloses a method for identifying the suitability of heterologous sperms in gynogenesis breeding of micropterus salmoides, and particularly relates to the technical field of gynogenesis breeding and germplasm innovation of fishes. The method comprises the following steps: collecting sperms of different fishes; fixing the collected fish seminal fluid with glutaraldehyde, preparing a sample, and observing with a scanning electron microscope; according to a scanning electron microscope result, preliminarily screening heterologous sperms with unobvious structure and size differences, and carrying out a hybridization pre-experiment on the heterologous sperms and largemouth bass ova; observing the embryonic development condition of each hybrid combination; and screening out heterologous sperms which can normally fertilize ova of micropterus salmoides but cannot obtain filial generations as gynogenesis heterologous sperm donors, and carrying out gynogenesis practical operation verification. According to the method, the optimal gynogenesis stimulation source is screened out, so that the breeding process of creating new germplasm through fish gynogenesis breeding is accelerated.

Texas rig fishing is an effective method of fishing, particularly suited for bass, that allows anglers to fish in bodies of water with significant submerged vegetation or other obstacles that may make maneuvering a traditional fishing line and lure difficult. For these applications, a weight is added to the line near the hook to improve the ability for the angler to control the depth of the lure and the maneuverability of the fishing lure. These weights are called bullets. The present invention relates to an improved bullet design that reduces the risk of the fishing lure being entangled by underwater vegetation or other obstacles, protects the soft plastic lure from wear, and prevents breakage throughout the entire fishing process while also removing the need to peg the bullet or use a bobber stop.

The invention discloses a method for regulating and controlling factory-like indoor natural oviposition of parent fishes of red pink bass, and relates to the technical field of breeding of red pink bass. The method comprises the steps of parent fish selection, parent fish seaborne net cage cultivation and indoor reproduction regulation and natural oviposition. According to indoor reproduction regulation and control of natural oviposition, conditions including water temperature, illumination period and nutrient enrichment for parent fish cultivation are regulated and controlled in a land-based factory-like indoor running water cement pond, parent fish gonad development and synchronous maturation are regulated and controlled, natural oviposition is achieved, and batches of high-quality fertilized eggs are obtained; according to the method, the parent fish can naturally lay eggs in the indoor breeding pond at the water temperature of 26-27 DEG C within the regulation period of one week, the egg laying period can reach 121 days, the fertilization rate reaches 95% or above, and the hatching rate reaches 85% or above. According to the method, factory-like indoor natural spawning of the pink pink bass is broken through for the first time, and batch breeding and breeding development of pink pink bass seedlings can be effectively promoted.

The invention relates to the technical field of fish food conversion domestication, and discloses an outdoor perch net cage food conversion domestication device and method.The outdoor perch net cage food conversion domestication device comprises an aisle plate, net cages and bearing platforms are fixedly connected to the two sides of the aisle plate, the bearing platforms are located at the tops of the net cages, and bottom plates are fixedly connected to the two sides of the top of the aisle plate; water spraying assemblies and lock catches are arranged on the two sides of the aisle plate, one end of each lock catch is fixedly connected to the outer wall of the aisle plate, the outer wall of each lock catch is sleeved with a pull rope, and each water spraying assembly comprises a first water spraying head and a water pump. The feed is pushed to move by utilizing impact force of water flow, the effect of simulating the dynamic state of live baits is achieved, the domestication problem that carnivorous fishes such as perches only eat live baits and do not eat static artificial feed is solved, the domestication effect is remarkably enhanced, the feeding habit conversion process of the carnivorous fishes is accelerated, in addition, timed and quantitative feeding can be achieved, labor is saved, and the domestication efficiency is improved. The food conversion domestication effect is also obviously enhanced, and the fish food habit conversion process is accelerated.

The utility model belongs to the technical field of weever growth monitoring, and particularly relates to a portable fry proofing and catching device. The fishing device comprises a telescopic rod and a fishing net, the fishing net is of a quadrilateral structure, a plurality of buoys are evenly fixed to the upper side edge of the fishing net, and a plurality of lead weights are evenly fixed to the lower side edge of the fishing net; the four corners of the fishing net are each fixedly provided with a fixing rope in an extending mode, and the fishing net is fixed between the tops of the two telescopic rods through the fixing ropes. The micropterus salmoides fry sampling fishing net is simple in structure, low in fishing cost, convenient to carry and high in operability, an operator can give an initial speed to the fishing net through the telescopic rod to help the fishing net sink into water quickly during sampling fishing, the escape time of micropterus salmoides fries is shortened, sampling fishing can be completed without cooperation of other related equipment, and the working efficiency is improved. And the micropterus salmonides with high speed can be caught easily, and the proofing effect of the growth health condition of the micropterus salmonides is guaranteed.

The utility model relates to the field of fishery aquatic tools, in particular to a catching device for breeding largemouth black bass, which comprises a bottom plate, a top plate, a plurality of upright posts, a plurality of metal nets, a grading mechanism and a blocking mechanism, a fish inlet is mounted on one metal net, the grading mechanism comprises a driving component, a connecting component, a guide component and a plurality of grading nets, and the blocking mechanism is mounted on the top plate. The blocking mechanism comprises two baffles and a telescopic assembly, when the driving assembly works, the multiple grading nets can be driven to move in the fishing box composed of the multiple metal nets, the largemouth black bass fished in the fishing box is extruded, the swimming space of the largemouth black bass is reduced, the small largemouth black bass can sequentially penetrate through the multiple grading nets, and the fishing effect of the largemouth black bass is improved. And therefore, grading of the largemouth bass is completed, rapid separation of fish bodies of different specifications is achieved, manual follow-up sorting is not needed, and the overall efficiency of fishing operation is improved.

The invention discloses an application of domoic acid in preparation of a product for treating or preventing iridovirus infection of fish micropterus salmoides, and claims to protect an application of domoic acid or a substance taking domoic acid as a main component in preparation of a product for treating or preventing iridovirus infection of fish micropterus salmoides. The molecular formula of the domoic acid is C15H21NO6. Fish body experiments prove that DA is the protection effect of the LMBV inhibitor on the survival rate of the fish body, and discovers that after DA is added into the fish body infected with LMBV, the death rate is reduced by 12.5%, and a certain protection effect is achieved.

The utility model is applicable to the technical field of fishery breeding, and provides a weever hatching barrel with a monitoring function, which comprises a support plate, a hatching barrel for hatching weever roes is fixedly mounted on the support plate, and an annular cavity is arranged on the hatching barrel; the box body is fixedly installed on the supporting plate, a water pump is fixedly installed on the inner wall of the bottom of the box body, a water drainage pipe is fixedly installed at the water drainage end of the water pump, and one end of the water drainage pipe extends into the hatching barrel; the overflow pipe is fixedly mounted on the box body, one end of the overflow pipe extends into the hatching barrel, and an egg blocking net is fixedly mounted at one end of the overflow pipe; and the oxygen delivery pipe is fixedly arranged on the hatching barrel. According to the scheme, the problems that an existing weever hatching barrel wastes water sources in the using process, and low-temperature water cannot be automatically heated are solved.

This invention relates to the field of aquatic feed technology, specifically to a largemouth bass and black soldier fly oil feed and its preparation method, which is made by mixing basic components, oil source components, additives and functional components. The basic components include the following ingredients in parts by weight: 30-40% fish meal, 3-8% soybean meal, 15-25% chicken meal, 3-8% corn gluten meal, 10-15% cottonseed protein, 4-10% wheat flour and 2-6% cassava starch. The oil source components include the following ingredients in parts by weight: 1-2% soybean oil, 2-5% black soldier fly larvae oil and 1-3% Schizochytrium oil. The functional components include the following ingredients in parts by weight: 0.5-1.5% premix, 0.5-1.0% choline chloride, 0.05-0.2% vitamin C, 0.1-1.0% DL-methionine and 0.05-0.2% ethoxyquin. The fish oil-free compound oil feed of the present invention can not only replace fish oil, but also supplement n-3 LC-UFA through Schizochytrium oil, which can improve the problem of insufficient deposition of unsaturated fatty acids in muscle and deterioration of muscle fiber structure caused by the use of black soldier fly larvae oil alone, thus improving both economic benefits and meat quality.

This utility model relates to the field of bass farming technology, specifically a bass farming box with an automatic cleaning function, comprising: a tiered farming component and a cleaning component. The tiered farming component consists of a box body, limiting blocks, tiered plates, and a filter screen. The limiting blocks are installed on the inner wall of the box body. This bass farming box with automatic cleaning function, through the arrangement of the box body, limiting blocks, tiered plates, and filter screen, allows for tiered farming of bass by fixing the limiting blocks to the box body and connecting the tiered plates. The equidistant arrangement of the tiered plates facilitates the flow of water into all parts of the box, allowing bass of different sizes to be farmed in different water layers. For example, smaller fry can be farmed in the upper layer, while larger adult fish can be farmed in the middle or bottom layer. This fully utilizes the vertical space of the farming water body, increasing the farming density per unit water volume. Tiered farming improves space utilization and increases farming density.

The present application belongs to the field of fish molecular immunology, and particularly relates to an oral vaccine containing a largemouth bass iridovirus ATPase and a preparation method thereof. B. subtilis The spores of the WB600 strain are antigen delivery carriers, the spore coat protein C (CotC) is used as an anchor protein, and the key protein of the largemouth bass iridovirus is fused with the ATPase gene and CotC the gene to construct a recombinant spore surface display system, so that the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. The vaccine can tolerate the gastrointestinal environment after being orally administered to fish, and is accurately delivered to the gut-associated lymphoid tissue to stimulate the immunity of the fish, so that the prevention and control of the largemouth bass iridovirus is finally achieved.