5results about How to "Improve hatchability" patented technology

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.

This utility model discloses a black soldier fly egg collection device, comprising a frame, wherein an attraction lamp or attraction box is provided in the frame, and an attraction agent is provided in the attraction box. Multiple collection troughs are arranged sequentially from top to bottom within the frame, with a spacing of 8mm to 15mm between adjacent collection troughs. Each collection trough consists of upper and lower collection strips, with a spacing of 2mm to 4mm between the upper and lower collection strips. The sequentially arranged collection strips facilitate the female black soldier fly to climb on both sides and lay eggs in both directions. The 8mm to 15mm spacing between adjacent collection troughs ensures that the female black soldier fly lays eggs only within the space of the collection trough, thereby improving the egg hatching rate.

The application provides a strong diurnal temperature range water area schizothorax ova low temperature-high temperature stimulation membrane rupture and double high water temperature tolerance threshold experiment method, including: step one, determining the diurnal extreme value and amplitude of the hatching water temperature of the schizothorax ova; step two, realizing the diurnal amplitude hatching water temperature; step three, key water temperature control of membrane rupture; step four, low temperature-high temperature stimulation large-scale membrane rupture; step five, determining the double high water temperature tolerance threshold. The application determines the diurnal extreme value and amplitude of the hatching water temperature of the natural reproduction ova, designs different diurnal amplitude hatching water temperature experiment hatching ova, simulates the natural hatching environment of the Qinghai-Tibet Plateau river with large diurnal water temperature difference under climate change; through the indoor ova water temperature gradient experiment, the low temperature-high temperature stimulation membrane rupture mechanism of the Schizothorax grahami, the double high water temperature tolerance range and threshold of the key fish in the source region of the Yangtze River are found, and scientific support is provided for the protection and restoration of the fish resources in the source region of the Yangtze River under the background of climate warming.

This invention belongs to the field of acidifier technology, specifically relating to an acidifier that can improve the health of breeding ducks and the hatchability of hatching eggs, its preparation method, and its application. The acidifier includes acidifier A and acidifier B. Acidifier A is prepared by mixing and adjusting the volume of calcium formate, acetic acid, lactic acid, monk fruit extract, and an immune enhancer; acidifier B is prepared by mixing and adjusting the volume of calcium propionate, citric acid, malic acid, stevia extract, and an antioxidant. This invention enhances the immunity and antioxidant capacity of breeding ducks and improves eggshell quality by intermittently and alternately adding the two acidifiers to their drinking water during the egg-laying period. Furthermore, by spraying a proportionally diluted mixture of the two acidifiers during the later stages of hatching, it improves embryonic calcium absorption and duckling shell-pecking rate, reduces embryonic mortality, and significantly increases the hatchability of hatching eggs. This invention is suitable for large-scale breeding and hatching of breeding ducks.