40 results about "Bovine collagen" patented technology

Bovine collagen is freed of non-collagen proteins, glycosaminoglycans and lipids by microbial treatment to yield a product, which is undenatured fibre of high tensile strength. The microbial extraction technique ensures degradation of non-collagenous components with the help of the protease combination produced, leaving the collagenous matter intact due to the absence of collagenase in the secreted enzymes. The resulting atelopeptide collagen has largely monomeric triple helical conformation. This process results in the formation of regularly ordered fibres of collagen possessing a rope-like structure. It is soluble in dilute acidic aqueous solutions. The collagen is rendered non-immunogenic by the removal of certain terminal peptide chains. The non-cytotoxic fibres can be fabricated into various physical forms for biomedical applications.

The invention provides a sequential extraction process for bovine collagen peptides, which comprises the following steps: performing primary extraction by adopting ultrasonic waves; performing solid-liquid separation to obtain filtrate and filter residue; performing primary enzymatic hydrolysis to obtain a primary enzymatic hydrolysate; performing enzyme deactivation, separating undissolved substances, and removing impurities to obtain a primary refined solution; performing concentration treatment; performing sterilization and drying to obtain primary protein peptide powder; performing secondary enzymatic hydrolysis to obtain a secondary enzymatic hydrolysate; performing concentration treatment; performing sterilization and drying to obtain secondary protein peptide powder. According to the sequential extraction process for bovine collagen peptides, the primary extraction is performed in an alkalescence environment with the pH value being 7-8, the extraction efficiency is improved under a 0-0.04 Mpa micro-high-pressure environment through matching of the operation temperature being 100-105 DEG C, the collagen peptide purity and the molecule integrity are ensured, and the protein utilization ratio is relatively high; the extraction efficiency (production efficiency) is improved through the combination of ultrasonic wave extraction, and acids, alkalies and other chemical reagents are avoided from use and emission, so that the process is friendly to the environment and does not pollute the environment.

The invention provides a bovine bone collagen peptide production device. The wall of a tank body comprises an outer wall, a heating plate and an inner wall from outside to inside, the heating plate iscontrolled by an independent unit, a heating area can be adjusted according to a liquid surface of reactant in the tank body, so that the energy consumption is reduced; meanwhile, the connection anddisconnection of an electromagnet facilitates the fixation and disassembling of the heating plate, and far infrared rays released by graphene has a catalytic effect for enzymolysis reaction in the tank body, so that the enzymolysis efficiency and speed can be effectively improved. In addition, various tank bodies are connected with one another by virtue of the cooperation of annular bars and annular grooves and are fixed by virtue of fasteners, and the fasteners comprise a second crossbar, a connecting rod and a first crossbar which are connected with one another in a pivoting manner. The production device is convenient to disassemble and assemble and convenient and flexible to use, and can adjust the number of tank bodies according to the enzymolysis times, thereby reducing the process complexity in the production process.

The invention belongs to the technical field of protein peptide preparation, and in particular relates to a production process of bovine bone collagen peptide. The production process of the bovine collagen peptide provided by the present invention includes steps such as S1, raw material pretreatment; S2, oil-water separation; S3, enzymatic hydrolysis; S4, microwave enzyme inactivation; S5, purification; The bovine collagen peptide powder prepared by the bovine collagen peptide production process provided by the present invention has the advantages of small molecular weight, concentrated distribution, fast absorption, low fat content, etc., and has good water solubility, and is not easy to produce precipitation when dissolved in water. High utilization, long-term use can effectively improve human immunity, relieve physical fatigue, and can be widely used in fields such as health food, formula food and cosmetics.

Provided are synthetic bone graft substitutes that include bioactive glass and a carrier. Synthetic bone graft substitutes may include bioactive glass, glycerol and polyethylene glycol. Also provided are bone graft substitutes that include collagen and bioactive glass particles. Example bone graft substitutes may include collagen and bioactive glass particles. Other example embodiments may include Type I Bovine Collagen, an angiogenic agent, such as hyaluronic acid, and bioactive glass. Further provided are methods that include administering the present bone graft substitutes to a mammal, e.g., by surgical insertion of the bone graft substitute into the mammal, either alone or in conjunction with one or more implant devices. Further provided are kits that include the present bone grafts.

The invention belongs to the technical field of enzymatic hydrolysis of bovine collagen peptides, and in particular relates to a production process of bovine collagen peptides, including S1 cleaning, S2 denaturation, S3 enzymatic hydrolysis, S4 centrifugation, S5 enzyme inactivation, S6 impurity removal, and S7 finished product. The present invention has the following advantages and effects: the application uses ultrasonic technology, denaturation technology and enzymatic hydrolysis technology to work together, which can be conveniently adapted to cowhide from various origins; and the collagen peptide product produced by the technology provided by the application has good dissolution properties and dispersibility, good sensory quality, high yield, and a large amount of active polypeptides in the product make it have strong antioxidant capacity; the method of this application has relatively mild reaction conditions, low production energy consumption, and far superior process conditions and process effects in existing technical solutions.

The invention discloses a beef tendon rinsing and roasting slice processing method. By adoption of traditional food materials and an advanced enzymatic reaction technique, intrinsic collagen and protein of the raw materials are subjected to cross-linking reaction with substances such as external cattle collagen, then modern slicing equipment is adopted for accurate shaping and slicing, and high collagen content is realized. In addition, beef tendon rinsing and roasting slices processed according to the method is chewy in taste, rich in nutrition, convenient to consume and suitable for cold dishes, rinsing, roasting and the like.

The invention relates to a collagen temperature-sensitive hydrogel and a preparation method thereof Soft tissue filling materials utilized in the existing plastic and beauty treatment industry have the problems of hardening, production of phyma, too fast absorption speed, and induction of chronic inflammation. American F Dalton approves successively multiple products for soft tissue filling, and main components of the products comprise bovine collagen, hyaluronic acid sodium, polymethyl methacrylate (PMMA) globules and the like. The products have good effects but can still produce cases of redness and allergy of a used position and too fast absorption. In the invention, collagen solution, chitosan acid or hyaluronic acid, and beta-sodium glycerophosphate solution are mixed according to a volume ratio of (1 to 3): (1 to 5): (1 to 2), then are stirred well at a temperature of 0 to 15 DEG C for 10 to 30 minutes and are adjusted to a pH of 7.4 to form temperature-sensitive hydrogel. The temperature-sensitive hydrogel has the advantages of good gel forming property at a temperature of 37 DEG C, gel forming time within 10 minutes, good mechanical strength, long degradation time, low immunological rejection, and favorable safety and effectiveness.

The invention discloses a method for producing ox collagen by means of tail-end gelatin of oxhide gelatin, and belongs to the field of oxhide processing. According to the method, through steps of ethyl alcohol pre-treatment, enzymolysis, enzyme removal and the like, the collagen is extracted, through ethyl alcohol, pre-treatment can be conducted on the tail-end gelatin easily, impurities in the tail-end gelatin are removed, the purity and the quality of the collagen are improved, enzymolysis time is shortened, by adjusting an enzymolysis sequence, the molecular weight of the collagen is controlled, collagen products with molecular weight between 3 KDa to 8 KDa can be prepared, and the molecular weight of the prepared collagen is centralized, and can account for more than 70% of total molecular weight of collagen; a whole preparation process can be completed in 15-20 h, so that compared with the prior art, the method for producing the ox collagen by means of the tail-end gelatin of theoxhide gelatin has the advantage that the time cost is greatly saved.

The invention relates to the technical field of animal food processing, in particular to a bovine collagen peptide capable of relieving fatigue and improving bone density and a preparation method thereof. The preparation method of the bovine bone collagen peptide of the present invention comprises the following steps: using bovine bone as a raw material, enzymatically hydrolyzing the pretreated raw material to obtain an enzymatic hydrolyzate; the compound protease is neutral protease, papain and flavor Protease. The bovine bone collagen peptide prepared by the method has a high content of small molecular peptides; it has good water solubility, is easily absorbed by the human body, and has no bad smell; it has good effects of relieving fatigue and improving bone density; and the preparation method is simple and convenient to operate. Industrial production.

The invention belongs to the field of collagen peptide, and discloses bovine bone collagen peptide and a production method thereof. The production method of the bovine bone collagen peptide comprisesthe steps of impurity removal, smashing and cooking on bovine bone, oil-water separation for cooking liquid, high-efficiency compounding of enzymic preparations, enzymolysis on the condition that thepH value is not adjusted and the temperature condition is not controlled, filtering of an inorganic nanofiltration ceramic membrane to remove components which are easily dissolved and components whicheasily absorb moisture, high-temperature and high-pressure treatment, concentration and drying for powder preparation. According to the method, the enzymolysis pH value does not need to be adjusted,the enzymolysis temperature does not need to be controlled, the enzymolysis time is short, the enzyme addition amount is small, and the enzymatic products are low in molecular weight and are centralized; meanwhile, the prepared bovine bone collagen peptide powder is excellent in water re-dissolubility, and does not easily absorb moisture or get caked in the storage process.

In the present invention, the porcine decellularized dermal matrix is ​​prepared by placing the dermal matrix in a 0.5Mol / L sodium hydroxide solution prepared with triple-distilled water and continuously shaking it, then rinsing it with triple-distilled water and phosphoric acid balanced saline, and freeze-drying , packed and sterilized; pig acellular dermal matrix is ​​used as a scaffold for in vitro culture of epidermal cells to prepare tissue-engineered composite skin. After the dermal matrix is ​​coated with type I bovine collagen dissolved in acetic acid, the pH is adjusted to 7.0-7.4, and then placed successively Soak in phosphate balanced salt solution and serum-free medium, take out the inoculated and passaged epidermal cells, and then place them in CO 2 Serum-free culture in the incubator; application of porcine acellular dermal matrix, involving the construction of tissue-engineered composite skin with porcine acellular dermal matrix as a scaffold and the preparation of tissue-engineered composite skin as a substitute for human skin.