33 results about "Simmental cattle" patented technology

The invention discloses a Chinese Simmental cattle FGF-1 gene as genetic markers of carcass meat quality. According to the method, the Chinese Simmental cattle genome DNA or a DNA sequence comprising the Chinese Simmental cattle FGF-1 gene is taken as a template, the functional region segment of the Chinese Simmental cattle FGF-1 gene is amplified by use of PCR primer pairs P1, P2 and P3, enzyme digestion and electrophoretic analysis are performed on restriction enzymes Xsp-I, Hae-III and Rsa-I by virtue of sequencing screening, and then the SNPs at the 8528th site, the 13970th site and the 72251th site of the second introns of the Chinese Simmental cattle FGF-1 gene can be determined (A is greater than T, A is greater than T and A is greater than C). The T allel gene and the TT genotype of the SNP1 site, the C allel gene and the CC genotype of the SNP2 site and the T allel gene and the TT genotype of the SNP3 site in the SNPs obtained by screening can be taken as effective molecular genetic markers for remarkably improving the carcass composition and meat quality traits of the Chinese Simmental cattle, and then can be used for selective molecular breeding under the assistance of early marking of the carcass composition and meat quality traits of the beef cattle.

The invention provides a group of microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle. The group of microsatellite markers includes ten microsatellite markers. The microsatellite markers have the advantages that the markers are high in polymorphism and are not linked with one another, fragments are appropriate in size and interval, and simultaneous detection can be facilitated; combined exclusion probabilities CPE (1), CPE (2) and CPE (3) can respectively reach 0.9960969, 0.999854 and 0.9999997 when the parent-child relationships of the Chinese Simmental cattle are identified by the aid of marker combinations under three different conditions, and accordingly the microsatellite markers are extremely high in detection efficiency; the microsatellite marker combinations can be used for completely and accurately identifying family trees of the Chinese Simmental cattle, accordingly, the Chinese Simmental cattle breeding accuracy can be improved, breeding procedures can be accelerated, and the microsatellite markers have excellent application prospects and economic benefits.

The invention discloses a detection method of Chinese simmental cattle carcass and meat quality trait genetic marker, and the method uses DNA pool sequencing and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology for screening and detection of GPAM (glycerol-3-phosphate acyltransferase, mitochondrial) gene function mutation of Chinese simmental cattle. Through the correlation analysis, Chinese simmental cattle carcass and meat quality trait-related SNPs as a molecular genetic marker is found, and is used in the early selection and molecular pyramiding breeding of beef cattle, and breeding and improving of meat use performance of the Chinese simmental cattle can be further accelerated.

The invention discloses an FDFT1 (farnesyl-diphosphate farnesyltransferase 1) gene key loci affecting Chinese simmental cattle fat deposition, and relates to a detection method for single-nucleotide polymorphism of Chinese simmental cattle fat deposition trait related FDFT1 gene. The method takes Chinese simmental cattle genomic DNA or a DNA sequence containing the Chinese simmental cattle FDFT1 gene as the template, and utilizes the PCR primer pair P1 and P2 to amplify a Chinese simmental cattle FDFT1 gene functional region fragment, and by means of sequencing screening, restriction endonuclease Hae-III and Hpa-II enzyme digestion, and electrophoretic analysis, the 130th SNP1 (T greater than C) of a 4th intron in the Chinese simmental cattle FDFT1 gene and the 500th SNP2 (C greater than T) of a 8th exon can be determined. In the screening obtained SNPs, the T allele and TT genotype of the SNP1 locus and the C allele and CC genotype of the SNP 2 locus can serve as the effective molecular genetic markers for significantly improving Chinese simmental cattle fat deposition and inter-muscular fatty acid traits so as to be used for beef cattle meat quality trait early marker assisted selection of molecular breeding.

The invention relates to a hybridization cattle fattening method which includes the following steps: (1) selecting a hybridization cattle with a Pingwu cattle as a female parent and a Simmental cattle as a male parent, (2) expelling parasites, (3) feeding the cattle with the specially-manufactured total mixed ration, (4) conducting fattening under the drylot feeding condition, and (5) measuring the daily-increased weight, the feeding consumption and economic benefits. Compared with a fattening cattle in a traditional set, the fattening cattle fattened with the method has the advantages that the daily-increased weight is improved by 10.30%, the feeding consumption is reduced by 12.70%, and gross incomes are improved by 33.89%.

The invention relates to feed and in particular relates to environment-friendly compound feed for Simmental cattle at growing stage. The feed is prepared from the following raw materials in parts by weight: 80-90 parts of yellow corn, 2-3 parts of baking soda, 8-12 parts of barley bran, 18-20 parts of bean cake, 4-5 parts of vinegar residue, 12-15 parts of wheat straw, 5-8 parts of watermelon seed, 12-15 parts of Mexican teosinte, 8-12 parts of awnless brome, 2-3 parts of fine bamboo charcoal powder, 1-2 parts of calcium hydrophosphate, 3-4 parts of dried laver powder, 4-5 parts of garlic residue, 4-5 parts of fresh burdock, a proper amount of salt and 4-5 parts of a phagostimulant. According to the feed, the raw materials such as the yellow corn, wheat bran and Mexican teosinte are subjected to ensiling treatment, so that the feed is high in palatability and easy to digest, the raw materials such as the watermelon seed, dried laver powder and fresh burdock contain rich proteins and trace elements, the weight gain of the cattle can be accelerated, the feed weight rate is reduced, and the feed utilization rate is high. In addition, hormone and other medicines are not added, and the product is green and safe.

The invention provides a daily ration formula for beef cattle fattening with silage sorghum addition and application of the formula. According to the present invention, the ideal fattening effect of beef cattle can be achieved, the comprehensive utilization efficiency of sweet sorghum is improved, and the feeding cost is reduced, by scientifically allocating the daily ration formula with sweet sorghum in the cattle feeding process, scientifically screening premix and concentrate supplement, optimizing and obtaining the best scheme of the daily ration formula for beef cattle fattening, and formulating scientific and reasonable management regulations for cattle feeding with sweet sorghum at the same time. The daily ration formula of the invention is used to feed Simmental cattle, and the test results show that the group of 50% silage sorghum + 50 silage whole plant corn has the best daily weight gain and the highest efficiency of feed conversion, and the daily weight gain is 1.74 kg; andthe slaughter performance of 50% silage sorghum + 50 silage whole plant corn is higher, the slaughter rate is 57%, and the highest digestibility is 59.22%. The results indicate that the meat yield ofbeef cattle can be improved by replacing part of silage whole plant corn with silage sorghum.

The invention relates to an online measurement method for body size of living Simmental cattle based on a 3D camera, and the method comprises the steps: obtaining the three-dimensional image data of acattle body through a TOF camera, and converting the three-dimensional image data of the cattle body into the three-dimensional point cloud data of the cattle body; processing the cattle body three-dimensional point cloud data by using conditional filtering, statistical filtering and voxel filtering; performing clustering segmentation on the filtered cattle body three-dimensional point cloud databased on a random sampling consistency algorithm and an Euclidean clustering algorithm; performing curved surface reconstruction on the cattle body three-dimensional point cloud data obtained in theprevious step to obtain a complete cattle body curved surface model; on the reconstructed cattle body curved surface model, selecting body size measurement points according to a preset selection condition to obtain seven-item body size data corresponding to the cattle body; correcting the seven-item body size data by utilizing a correction function; according to the method, accurate measurement ofseven body size data of the cattle body is realized, and non-contact measurement of the body size of the living Simmental cattle is realized.

The invention discloses a cultivation method of beef cattle hybridized by Charolais cattle, Simmental cattle and local cattle. The method includes: selecting a Tongjiang cow as the female parent; performing superovulation on the Tongjiang cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the Tongjiang cow; selecting a Charolais surrogacy cowto perform embryo transplantation; detecting 2 genotypes related to the growth traits of a first-filial-generation cow; selecting an F1-generation cow as the female parent; performing superovulation on the F1-generation cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the F1-generation cow; selecting the first-filial-generation cow to perform embryo transplantation; detecting 2 genotypes related to the growth traits of a second-filial-generation individual; selecting the cattle, which is good in body shape and growth speed and has dominant genotypes, hybridized by Charolais cattle, Simmental cattle and local cattle, and using the artificial insemination, superovulation and embryo transplantation technologies to expand propagation. Bythe method, the workload of scientific and technical personnel can be lowered, and cultivation time can be shortened.

The invention discloses a hybridization and improvement method for Chinese simmental cattle. The Chinese simmental cattle serve as female parents, cattle except for the Chinese simmental cattle are selected as male parents, seminal fluid of the male parents is taken for mating with the female parents for hybridization, and filial generations are obtained; cows with excellent characters in the filial generations are selected and subjected to rotational crossing with the male parents till a new strain with excellent characters is obtained. According to the hybridization and improvement method for the Chinese simmental cattle, the similarity between blood of the Chinese simmental cattle and blood of purebred simmental cattle can be reduced, the phenomenon of degradation of the production performance of the Chinese simmental cattle is effectively controlled, and the new strain of the simmental cattle with excellent meat type or milk type or meat and milk type characters is bred.

The invention provides an SNP site related to the shank and beef knuckle weights on a No.6 chromosome of a simmental cattle for meat, and application of the SNP site. The SNP marked site is the 38576012 nucleotide site on the No.6 chromosome of the international cattle reference genome UMD3.1 version, and a basic group of the site is A or G. By optimizing dominant alleles of SNP, the dominantallele frequency can be increased generation by generation, meanwhile, the shank and beef knuckle weights of the simmental cattle for the meat can be increased, the cattle genetic improvement progressis accelerated, and thus economic benefits of beef cattle breeding are effectively increased.

The invention discloses a method for identifying bull breeds with genetic markers of mononucleotides of Y chromosomes of Bos Taurus. In the method, according to a genome resequencing result, DNAs of Y chromosomes of main beef cattle and dairy cattle breeds are scanned, so that SNP (single nucleotide polymorphism) sites of specific Y chromosomes of Holstein cattle, Red Angus cattle, Charolais cattle, Gelvieh cattle, Limousin cattle, Simmental cattle, Han cattle and Hareford cattle breeds are discovered, and different breeds of bulls are identified in the DNA level according to the SNP sites. Therefore, the method can be effectively used for introduction and molecular mark assisted breeding of all the breeds, and a foundation is laid for fast establishment of a Bos Taurus population with excellent genetic resources.

The invention provides an SNP site related to the forehoof weight on chromosome 6 of a simmental cattle for beef, and application of the SNP site. The SNP marked site is a 38815034 nucleotide siteon the chromosome 6 of an international cattle reference genome UMD3.1 version, and a basic group of the site is A or G. By optimizing the advantage allele of SNP, the frequency of the advantage allele can be increased by generations, the forehoof weight of the simmental cattle for the beef is increased, the development of cattle genetic improvement is accelerated, and thus the economic benefitsof beef cattle breeding are effectively increased.

The invention discloses application of ANGPTL5 gene as a bovine backfat thickness molecular marker and a detection method. After bovine genome-wide DNA is subjected to PCR (polymerase chain reaction) amplification, SNPs (single nucleotide polymorphisms) present in a PCR product sequence are detected through direct sequencing; it is discovered that four SNPs on an intron 1 and an intron 2 in the ANGPTL5 gene sequence are associated with bovine backfat thickness traits. A genetic marker in tight relation with bovine backfat thickness traits is screened and detected effectively, quickly and simply at DNA level; the genetic marker is applicable to molecular breeding of beef cattle; in initial stage of cattle breeding, the functional SNPs are used as markers for molecular breeding and assisted selection for modifying backfat thickness traits of Chinese Simmental cattle; bovine backfat thickness can be assessed and predicted quickly; therefore, with the cost reduced, beef quality is further improved, breeding speed of fine varieties is increased, and population quality is improved.

The invention provides an SNP locus relevant with spleen weight on the chromosome 6 of meat Simmental cattle and application of the SNP locus. The SNP marker locus is the 38852378th nucleotide locus on the chromosome 6 of UMD3.1 version of national cattle reference genome assembly, and the base of the locus is G or A. By optimizing the preferential alleles of the SNP, the frequency of the preferential alleles can be increased generation by generation, the spleen weight of the meat Simmental cattle is increased, and a genetic improvement progress of the Simmental cattle can be accelerated, so that the economic benefits of breeding beef cattle are effectively increased.

The invention discloses a method for detecting a fat coverage rate of Simmental cattle carcass by using DLK1 (Dalta Like non-canonical Notch ligand 1) gene markers. The method comprises the followingsteps: designing a pair of primers by using a DNA sequence of DLK1 gene of a Chinese Simmental cattle; performing strict PCR amplification and electrophoresis of samples on a PCR reaction system; screening and finding out two SNP sites by sequencing; screening from a population to obtain a restriction endonuclease Msp I at a DLK1-478 C / T site, wherein an excision base defined by the restriction endonuclease is CCGG; and screening to obtain a restriction endonuclease Ase I at a DLK1-609 T / G site, wherein T allele and TT mutant genotype individuals carrying the DLK1-478 C / T site in the Chinese Simmental cattle population have relatively high fat coverage rates and relatively good marbling; and G allele and GG mutant genotype individuals carrying the DLK1-609 T / G site have relatively high fatcoverage rates. According to the invention, the two mutation sites of the DLK1 gene can be used as genetic markers for predicting the fat coverage rate and marbling traits of the Chinese Simmental cattle, and are used for early marker assisted selection of beef cattle.

The invention relates to a fluorescent automated detection and analysis method of pathogenic sites of Arachnomelia syndrome (AS) in Simmental cattle. The nucleotide sequence of a primer for PCR (polymerase chain reaction) amplification is as follows: 5'-CTGAGTCTCCTCTTCTGTTTTCA-3',5'-GTTGGCATCTGAGTCCAGGT-3'. By using the fluorescent labeling primer PCR amplification and electrophoresis typing technology, the kit for typing pathogenic sites of AS in Simmental cattle has the advantages of stable result and low cost, and is accurate and reliable, thereby accurately typing the pathogenic sites of AS in Simmental cattle.

The invention provides an SNP locus related to hind leg length on the chromosome 6 of meat Simmental cattle and application of the SNP locus. The locus of an SNP marker is the 38696766th nucleotide locus on the chromosome 6 of the international cattle reference genome assembly UMD3.1 version, and the base of the locus is T or G. By optimizing the preferential alleles of the SNP, the frequency of the preferential alleles can be increased generation by generation, the hind leg length of the meat Simmental cattle is increased, and a genetic improvement progress of the cattle is accelerated, so that the economic benefit of beef cattle breeding is effectively improved.

The invention provides feed for beef cattle at the early fattening period. The early fattening stage means a stage that beef cattle has a weight of 140-210kg, and daily feed for each beef cattle comprises 1.4-1.6kg of concentrate supplement, 0.9-1.1kg of soy sauce residues, 0.9-1.1kg of vinegar residues, 1.4-1.6kg of straws and 5-7kg of silage. According to the feed for beef cattle at the early fattening period, regarding the growing characteristics of Simmental cattle at the early fattening period by adopting a constant fattening method, the feed has more comprehensive nutrition since the soysauce residues and vinegar residues are extra added on the basis of adopting a certain amount of concentrate supplement, silage and straw roughage, is favorable to growth of bones and skeletons of cattle, and has a better later fattening effect; and other raw materials in the feed except the concentrate supplement are natural and environment-friendly raw materials, so that cost can be saved, theimmunity of cattle can be enhanced, and the incidence rate can be reduced.

The invention relates to a fluorescent automated detection and analysis method of pathogenic sites of Arachnomelia syndrome (AS) in Simmental cattle. The nucleotide sequence of a primer for PCR (polymerase chain reaction) amplification is as follows: 5'-CTGAGTCTCCTCTTCTGTTTTCA-3',5'-GTTGGCATCTGAGTCCAGGT-3'. By using the fluorescent labeling primer PCR amplification and electrophoresis typing technology, the kit for typing pathogenic sites of AS in Simmental cattle has the advantages of stable result and low cost, and is accurate and reliable, thereby accurately typing the pathogenic sites of AS in Simmental cattle.

The invention discloses a method for detecting the fat coverage rate of Simmental cattle carcass by DLK1 gene markers. Using the DNA sequence of the DLK1 gene of Chinese Simmental cattle as a template, a pair of primers are designed, and a strict PCR reaction system is carried out to carry out PCR of samples Amplification and electrophoresis, screening by sequencing and finding 2 SNP sites; the restriction endonuclease for screening DLK1-478 C / T sites in the population is Msp I, and the cleavage base limited by this enzyme is CCGG; screening The restriction endonuclease of DLK1‑609 T / G site is Ase I; the individuals carrying DLK1‑478 C / T site T allele and TT mutation genotype in Chinese Simmental cattle population have higher fat coverage and better marbling; and individuals with the G allele and GG mutant genotype carrying the DLK1‑609 T / G locus had higher fat coverage. The two mutation sites of the DLK1 gene of the present invention can be used as genetic markers predicting the fat coverage rate and marbling traits of Chinese Simmental cattle, and used for early marker-assisted selection of beef cattle.

The invention provides an SNP locus related to hind leg length on the chromosome 6 of meat Simmental cattle and application of the SNP locus. The locus of an SNP marker is the 38696766th nucleotide locus on the chromosome 6 of the international cattle reference genome assembly UMD3.1 version, and the base of the locus is T or G. By optimizing the preferential alleles of the SNP, the frequency of the preferential alleles can be increased generation by generation, the hind leg length of the meat Simmental cattle is increased, and a genetic improvement progress of the cattle is accelerated, so that the economic benefit of beef cattle breeding is effectively improved.

The invention provides an SNP site related to the forehoof weight on chromosome 6 of a simmental cattle for beef, and application of the SNP site. The SNP marked site is a 38815034 nucleotide siteon the chromosome 6 of an international cattle reference genome UMD3.1 version, and a basic group of the site is A or G. By optimizing the advantage allele of SNP, the frequency of the advantage allele can be increased by generations, the forehoof weight of the simmental cattle for the beef is increased, the development of cattle genetic improvement is accelerated, and thus the economic benefitsof beef cattle breeding are effectively increased.

The present invention provides the SNP site on chromosome 6 of meat Simmental cattle related to spleen weight and its application. The site of the SNP marker is the 38852378th nucleoside on chromosome 6 of UMD3.1 version of the international bovine reference genome Acid site, the base of this site is G or A. By optimizing the dominant allele of the SNP, the invention can increase the frequency of the dominant allele generation by generation, increase the spleen weight of meat Simmental cattle, accelerate the progress of cattle genetic improvement, and effectively improve the economic benefits of beef cattle breeding.

The invention discloses a cultivation method of beef cattle hybridized by Charolais cattle, Simmental cattle and local cattle. The method includes: selecting a Tongjiang cow as the female parent; performing superovulation on the Tongjiang cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the Tongjiang cow; selecting a Charolais surrogacy cowto perform embryo transplantation; detecting 2 genotypes related to the growth traits of a first-filial-generation cow; selecting an F1-generation cow as the female parent; performing superovulation on the F1-generation cow; selecting Simmental breeding bull sperm to perform artificial insemination; collecting the embryo of the F1-generation cow; selecting the first-filial-generation cow to perform embryo transplantation; detecting 2 genotypes related to the growth traits of a second-filial-generation individual; selecting the cattle, which is good in body shape and growth speed and has dominant genotypes, hybridized by Charolais cattle, Simmental cattle and local cattle, and using the artificial insemination, superovulation and embryo transplantation technologies to expand propagation. Bythe method, the workload of scientific and technical personnel can be lowered, and cultivation time can be shortened.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving meat performance of beef cattle by using a g.42041062G>T site of an EDG1 gene. The method for improving the meat performance of the beef cattle by using the g.42041062G>T site of the EDG1 gene comprises the following steps of detecting whether mutation of the g.42041062G>T site exists in the EDG1 gene in a beef cattle genome to be detected: selecting a beef cattle individual with the genotype of TT, and carrying out artificial insemination and native mating. A molecular marker is used as a reliable marker for meat performance of Qinchuan cattle and Chinese Simmental cattle, and a technical system for detecting the genotype through DNA is established, so that the selection intensity and the breeding accuracy and efficiency are improved, and the meat performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.

The invention discloses a Chinese Simmental cattle FGF-1 gene as genetic markers of carcass meat quality. According to the method, the Chinese Simmental cattle genome DNA or a DNA sequence comprising the Chinese Simmental cattle FGF-1 gene is taken as a template, the functional region segment of the Chinese Simmental cattle FGF-1 gene is amplified by use of PCR primer pairs P1, P2 and P3, enzyme digestion and electrophoretic analysis are performed on restriction enzymes Xsp-I, Hae-III and Rsa-I by virtue of sequencing screening, and then the SNPs at the 8528th site, the 13970th site and the 72251th site of the second introns of the Chinese Simmental cattle FGF-1 gene can be determined (A is greater than T, A is greater than T and A is greater than C). The T allel gene and the TT genotype of the SNP1 site, the C allel gene and the CC genotype of the SNP2 site and the T allel gene and the TT genotype of the SNP3 site in the SNPs obtained by screening can be taken as effective molecular genetic markers for remarkably improving the carcass composition and meat quality traits of the Chinese Simmental cattle, and then can be used for selective molecular breeding under the assistance of early marking of the carcass composition and meat quality traits of the beef cattle.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for improving meat production performance of beef cattle by using a c.* 188G > A site of an AKIRIN2 gene. The method for improving the meat production performance of the beef cattle by using the c.*188G>A site of the AKIRIN2 gene comprises the following steps: detecting whether mutation of the c.*188G>A site exists in the AKIRIN2 gene in a beef cattle genome to be detected: selecting a beef cattle individual of which the genotype is AA type, and carrying out artificial insemination and natural indigo mating. According to the invention, the molecular marker is used as a reliable marker for the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle, and a technical system for detecting the genotype through DNA is established, so that the selection intensity and the breeding accuracy and efficiency are improved, and the meat production performance of the Qinchuan cattle and the Chinese Simmental cattle is improved.