The invention discloses a bovine 30K
liquid phase chip and application thereof, and belongs to the field of
molecular biology and biological chips. The
liquid phase chip comprises a probe combination for detecting 30715 SNP (
Single Nucleotide Polymorphism) loci, and the loci are obtained by performing whole
genome re-sequencing and screening on milk and meat dual-purpose type
Simmental cattle varieties such as Monebela cattle, Freevey cattle,
Simmental cattle and the like. The sites integrate paternity test sites, genetic defect
gene sites, variety identification sites and breeding target functional sites related to important economic traits such as
milk yield and meat traits. The
chip has high stability, high
detection rate and high accuracy, can be applied to paternity test, genetic defect screening, variety identification and
molecular breeding of milk and meat dual-purpose type
Simmental cattle varieties, and is particularly suitable for the requirements of small and medium-sized breeding enterprises on cost-sensitive accurate
genetic typing; and an efficient technical tool is provided for improved variety breeding of the milk-meat dual-purpose type Simmental cattle variety.