23results about How to "Accurate typing" patented technology

The invention relates to an antibody detection kit for neuroparatoma syndrome and a preparation method of the antibody detection kit. The antibody detection kit for neuroparatoma syndrome comprises an antibody detection strip, a cleaning solution, a buffer solution and an auxiliary agent, wherein the antibody detection strip is a nitrocellulose membrane which is coated with antigen proteins containing Amphiphysin, CV2, GAD65, Hu, PNMA2 (Ma2 / Ta), Recovery, Ri, SOX1, Titin, Tr (DNER), Yo and Zic4. The invention further discloses a detection method of the antibody detection strip. The antibody detection kit disclosed by the invention can be used for simultaneously detecting 12 autoantibodies related to the neuroparatoma syndrome. The design of simultaneous detection of multiple antibodies greatly improves the comprehensiveness of detection, so that multiple possible pathogenic factors can be covered in single detection, the kit can be used for differential diagnosis of suspected neuroparatoma syndrome patients in professional laboratories, and the problem of missed diagnosis caused by single antibody detection is avoided.

The present application relates to the field of DNA detection technology, and specifically to a kit, method and application for early screening and monitoring of HPV-related tumors. The present application is based on multiplex ddPCR and uses mixed probes to detect four HPV subtypes in plasma at one time. Compared with conventional detection technology, the kit is simple and safe to operate, non-invasive, low-cost, and has a short cycle; at the same time, the kit has higher detection sensitivity and strong specificity, with a minimum detection limit of only 250 copies / ml.

The invention discloses a microbial fermentation real-time monitoring and molecular analysis method based on a single-cell Raman spectrum technology. The method comprises the following steps: firstly, preparing basic gold nanospheres which are microcosmically spherical and have the particle size distribution of 20-40 nm through a chloroauric acid oxidation reduction method, and characterizing the morphology of the basic gold nanospheres; secondly, the surface of the basic gold nanosphere is coated with a silicon dioxide thin layer through tetraethoxysilane hydrolysis and polymerization reaction, and the silicon dioxide functionalized gold nanosphere with the core-shell structure is prepared to serve as the microorganism living cell probe. The probe and different microbial cells are co-cultured, Raman fingerprint spectrums of the cells are sensitively captured, the single-cell Raman fingerprint spectrums are deeply analyzed by adopting a machine learning technology, and differential analysis (including strain difference, environment difference, metabolic capability difference and the like) is combined to determine the single-cell Raman fingerprint spectrums of the cells. Molecular indexes of specific expression of the microbial cells in different industrial production processes and different culture periods are screened out. The invention provides a universal tool for accurately realizing real-time monitoring and molecular analysis of microbial cells in different industrial processes, and has a wide industrial application prospect.

This invention relates to a method for structural variant (SV) identification and genotyping in long-read family pedigree samples. The invention pertains to the field of vegetative variant (SV) detection in families, specifically focusing on methods for identifying and genotyping structural variants. The aim of this invention is to address the problems of existing family-based SV detection methods, which heavily rely on high-coverage sequencing, resulting in insufficient utilization of genetic characteristics and inaccurate SV detection and genotyping, as well as the high cost of sequencing multiple samples. This invention uses individual sequencing data from all family members as input, extracts variant features from each member, performs cluster analysis on the family feature set, assigns features to their respective members, and then uses three family feature signal correction methods to correct detection errors. Finally, SVs are located and anchored using Mendelian inheritance laws, and haplotype genotyping of SVs is completed using linkage information from long-read sequencing fragments.

The invention belongs to the technical field of novel nano functional materials and electrochemical biosensor detection, and discloses a preparation method of an Au (at) Co-NC NSAs / 3D GF composite material, the composite material and application of the composite material. The method comprises the following steps: (1) putting three-dimensional graphene foam into a mixed solution in which the concentration of cobalt nitrate hexahydrate is 0.0006 mol / L to 0.1 mol / L and the concentration of dimethylimidazole is 0.0006 mol / L to 0.1 mol / L, and keeping at room temperature for 2 to 12 hours to obtain ZIF-67 nanosheet array / three-dimensional graphene foam; (2) carrying out heat treatment on the ZIF-67 nanosheet array / three-dimensional graphene foam at the temperature of 300-900 DEG C for 1-5 hours to obtain cobalt nanoparticle anchored nitrogen-doped carbon nanosheet / three-dimensional graphene foam; and (3) measuring 5-50 [mu] L of a gold nanoparticle solution with carboxyl, and dropwise adding the gold nanoparticle solution on the surface of the cobalt nanoparticle anchored nitrogen-doped carbon nanosheet / three-dimensional graphene foam to obtain the gold and cobalt nanoparticle anchored nitrogen-doped carbon nanosheet / three-dimensional graphene foam. The method is simple in process, environment-friendly and high in yield.

The present invention belongs to the field of biotechnology, and relates to a composite amplification system and a kit of 23 short tandem repeat sequences, which are used to detect genetic marker genes with polymorphism in the human genome, and belongs to the field of biotechnology. The present invention relates to amplifying multiple short tandem repeat sequences simultaneously in a PCR system, and the specific loci are 22 short tandem repeat sequences with high genetic polymorphism and 1 sex determination site, and primers are designed and labeled with fluorescent groups respectively. The system has extremely high individual recognition rate and non-paternal exclusion rate, large amount of information, and good compatibility. The present invention can be used for forensic individual identification, paternity testing, and population genetics analysis, with higher accuracy and good sensitivity.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for detecting the color of watermelon pulp and application of the SNP molecular marker. The SNP molecular marker corresponds to a Chr06: 24308944 site on a No.6 chromosome of a watermelon Citrullus lanatus 97103 v2.5 genome, the polymorphism of the SNP molecular marker is G / A, and the SNP molecular marker is closely linked with the thick yellow / light yellow color character of watermelon pulp. When the basic group of the SNP site is G, the watermelon shows a thick yellow pulp character; and when the basic group is A, the watermelon shows a light yellow pulp character. The invention also provides a KASP primer group for detecting the SNP molecular marker, and a detection method based on the primer group, and KASP-PCR amplification and genetic typing are performed on a sample to be detected, so that the color character of watermelon pulp is judged. The invention also provides a kit containing the primer group and application of the kit in watermelon molecular marker-assisted breeding. The method has the advantages of being rapid in detection, high in accuracy, free of environmental influence, suitable for high-throughput application and the like, and the breeding efficiency of the yellow pulp watermelon variety is improved.

This invention discloses the KASP molecular marker for the wheat metsulfuron-methyl resistance gene TaASADH-B1 and its application, relating to a SNP site associated with wheat metsulfuron-methyl resistance in the field of marker-assisted breeding technology. The SNP site is located on wheat chromosome 5B, corresponding to the gene number TraesCS5B02G403400. The SNP site corresponds to the 101st base from the 5' end of SEQ ID NO: 1. When this site is homozygous for AA, the corresponding genotype is TaASADH-B1. A When this locus is homozygous for CC, the corresponding genotype is TaASADH-B1. C Wheat tolerance to mesosulfuron-methyl is characterized by the following genotype: TaASADH-B1 C Wheat tolerance is greater than or candidate greater than genotype TaASADH-B1 A The KASP marker developed in this invention is associated with wheat tolerance to the herbicide mesosulfuron-methyl and can be used for screening and identification of phenotypic traits of wheat varieties to tolerate mesosulfuron-methyl.

The present application is applicable to the field of bioinformatics, and provides an HLA type determination method and device, including: comparing and screening HLA second-generation sequencing data samples after quality control processing with gene features of an HLA type database, and gene classification of the screened data; determining the candidate type range of each gene according to the gene-classified data and the HLA type database; comparing the gene-classified data with a preset gene sequence to determine the difference site; chaining the difference sites into chains, and comparing them with the difference site database to obtain accurate candidate types; determining the HLA type result according to the candidate type range and the accurate candidate type; the present application can directly analyze the HLA type result for normal HLA second-generation sequencing data samples without manual intervention, and will give corresponding prompt information for abnormal data such as HLA new type samples, suspected mixed samples, and data unqualified samples.

The application provides an athlete muscle strength training and rehabilitation system based on electric stimulation, and relates to the technical field of medical rehabilitation.The application synchronously collects electromyography and kinematic signals, extracts muscle coordination index and joint net output torque double-dimension features in real time, identifies three movement performance states of coordination dominant type, strength dominant type and mixed type, and dynamically generates differentiated electric stimulation parameter sets according to the three movement performance states, so that the precise typing and targeted intervention of the fatigue state of athletes are realized, the muscle coordination efficiency and strength output are synchronously optimized in training, compensatory injury is effectively prevented and functional recovery is accelerated in rehabilitation, and the individualization, safety and overall efficiency of training and rehabilitation are improved.

The invention discloses an MGMT gene promoter methylation detection kit and application in temozolomide curative effect predication, the kit performs primer design for MGMT gene promoter methylation sites related to temozolomide curative effect predication, the detection result covers 12 CPG islands of an MGMT gene, and the accuracy is high. And the detection primer is a common PCR primer and is low in price. According to the present invention, the gold standard pyrosequencing is performed, the positive-negative determination standard for detecting the MGMT gene promoter methylation based on the nucleic acid mass spectrometry platform is constructed, and the kit can provide the MGMT degree so as to provide the reference for the temozolomide therapy drug.

The application belongs to the technical field of biology and particularly relates to a gene typing dot array chip for detecting African swine fever virus and a construction method and application thereof. The application firstly provides a single nucleotide polymorphism site combination for precisely detecting African swine fever virus of genotype I, genotype II and I / II recombinant virus. Secondly, the application provides a gene typing dot array chip for detecting African swine fever virus, which can simultaneously realize precise typing, epidemic and variation monitoring and identification of the recombinant gene of I / II recombinant virus of high-throughput detection samples.

This invention discloses a method for quantitatively assessing the severity of dark circles under the eyes, belonging to the field of objective evaluation technology in cosmetic dermatology. The method divides the eye into the upper and lower eyelids, each assigned a 50% weight. Each area is scored in three dimensions: area (A), color (D), and structure (S). A weighted total score for each eye and a combined total score for both eyes are calculated. Based on the total score, the severity is classified as mild, moderate, severe, and extremely severe. Furthermore, the percentage of each indicator is used to classify the condition as pigmentary, structural, and mixed types. This invention provides an objective, standardized, and repeatable assessment. The combination of multi-dimensional indicators improves the accuracy of the classification. It can be used for preoperative diagnosis and postoperative efficacy comparison, and is compatible with automated assessment in medical aesthetic systems, solving the technical problem of the lack of unified standards in traditional subjective assessments.

The present invention discloses a detection method and application of gene polymorphic sites related to sheep meat tenderness. The genetic polymorphism of sheep CCN5 gene in a population is analyzed by sequencing technology, and correlation analysis is performed by combining with individual meat quality trait data. It is found that the sheep population with superior meat tenderness can be selected early based on the polymorphic sites on the 5th exon of the CCN5 gene.

The invention belongs to the technical field of molecular biology and in-vitro diagnosis, and particularly discloses an LAMP (loop-mediated isothermal amplification) primer probe composition for detecting folic acid MTHFR (methylenetetrahydrofolate reductase) gene polymorphism, a kit and application of the LAMP primer probe composition. A probe-mediated LAMP melting curve analysis system is constructed, efficient nucleic acid amplification is realized under a constant-temperature condition by utilizing LAMP reaction, and rapid and accurate typing of C677T mutation sites is realized by combining high-specificity discrimination characteristics of a double-labeled probe melting curve. Compared with a traditional method, the method has the advantages of being short in reaction time, high in sensitivity, high in specificity, free of complex instruments and follow-up operation and the like, and is suitable for clinical rapid typing detection and basic molecular diagnosis application.

The invention relates to the technical field of biology and animal genetic breeding, and aims to provide a primer combination for detecting the genotype of a sheep Chr1: g.61541655 site, which is composed of the following three primers: a site A typing primer FAM with the sequence shown as SEQ ID NO: 1, a site B typing primer FAM with the sequence shown as SEQ ID NO: 2, and a site C typing primer FAM with the sequence shown as SEQ ID NO: 3. The sequence of the site T typing primer VIC is as shown in SEQ ID NO: 2; and the sequence of the universal primer is shown as SEQ ID NO: 3. T) of a sheep genome Chr1: g.61541655 site and the serum prolactin level are disclosed for the first time, it is clear that the AA genotype is a dominant genotype, and a reliable molecular target is provided for evaluating and screening sheep with high lactation potential from the genetic level.

This invention provides a SNP molecular marker associated with fatty acid content in sheep muscle and its application, relating to the field of biotechnology. Based on the discovered SNP site located in exon 1 of the SCARA5 gene, this invention not only enables rapid and accurate genotyping of the SCARA5 gene in sheep populations, but also, based on the significant correlation between this site and individual fatty acid content, allows for early screening of sheep with higher fatty acid content using SNP markers, thereby accelerating the breeding of superior sheep. Compared to traditional single-genotype selection, this invention offers higher accuracy and significant advantages in screening sheep with high fatty acid content, saving feeding costs and improving the breeding of superior sheep.

The present application belongs to the technical field of molecular markers and animal breeding, and particularly relates to a molecular marker related to the content of glycine, alanine and histidine in beef cattle muscle and application thereof. The present application provides a molecular marker located at the 18184950th base of chromosome 17 of a beef cattle reference genome GCF_002263795.3 version, having C / T polymorphism, which can not only realize rapid and accurate genotyping of beef cattle population SETD7 gene, but also based on the significant correlation between the site and the amino acid content of individuals, the beef cattle individuals with high amino acid content can be screened early, so as to improve the selection speed of superior beef cattle. Compared with the traditional single genotype selection, the present application has higher accuracy and significant advantages for screening beef cattle individuals with high amino acid content, saving feeding cost and improving the selection of superior beef cattle. The amino acid in the present application is one or more of glycine, alanine and histidine.

The invention discloses a meat duck body length character molecular marker as well as an identification primer and application thereof. The molecular marker is a duck No.28 chromosome chr28: 301790 locus, the locus has a T / C allele, and the corresponding genotypes are TT, TC and CC. The invention further provides an allele specific primer combination based on the KASP technology, the allele specific primer combination comprises an FAM primer, a VIC primer and a universal primer, and the specific sequences of the FAM primer, the VIC primer and the universal primer are shown as SEQ ID NO.1-3. According to the method, marker-assisted selection is carried out on 10 commercial lines of Huaifu meat ducks, TT type individuals are preferentially selected and reserved, CC type individuals are eliminated, the semi-submersible length is increased by 3.95 cm, the body weight is increased by 200 g, and the progress rate of breeding generations is increased by 38% or above. The method is simple and convenient to operate, low in cost and high in accuracy, and a molecular tool and a technical support are provided for early-stage precise breeding of body length characters of Huaifu meat ducks and similar strains.

This invention relates to the field of molecular biology, specifically to SNP molecular markers associated with palmitoleic acid trait in sheep muscle and their applications. Based on the detection of exons in the sheep SMAD1 gene, this invention identifies an SNP site at position 493 of exon 2 of the SMAD1 gene or position 13,440,579 of chromosome 17 of sheep. The base at this site is either A or G, and allele A is positively correlated with high palmitoleic acid trait in muscle. Furthermore, a method for detecting this SNP molecular marker is provided to achieve rapid and accurate genotyping of the SMAD1 gene in sheep populations (e.g., double-dried meat sheep). Moreover, based on the significant correlation between this site and individual palmitoleic acid and fatty acid content, SNP markers can be used to screen sheep individuals with higher palmitoleic acid content at an early stage, thereby improving the speed of breeding superior sheep.

The application discloses a kind of molecular inversion probes, molecular inversion probes based on mandarin fish genotyping chip and application, it is related to fish molecular breeding technical field, the molecular inversion probe of the present application sequentially includes left arm specificity sequence, general skeleton sequence and right arm specificity sequence from 5'to 3 ', general skeleton sequence includes molecular tag for high-throughput sequencing;The present application also provides the application including genotyping chip and in mandarin fish genotyping.The present application successfully designs and selects 10,000 specific probes, by verification, the chip system average enrichment success rate reaches 92.3%, and 53,454 high-quality single nucleotide polymorphism sites are captured, sequencing data comparison rate reaches 91.96%, target area is evenly covered, with the characteristics of high accuracy, high uniformity and high cost performance, provides efficient and reliable technical solutions for the molecular breeding and population genetics research of mandarin fish even other aquatic economic species.

The invention provides a synchronous detection primer for type II norovirus, group A rotavirus and adenovirus, which comprises specific primer pairs designed for conserved genes of three pathogens of type II norovirus, group A rotavirus and adenovirus, namely an ORF1 / ORF2 primer pair, a VP6 gene primer pair and a Hexon gene primer pair. The invention also provides a detection probe, which comprises an ORF1 / ORF2 capture probe, a VP6 capture probe and a Hexon capture probe. Meanwhile, the invention further provides application of the primer and the probe in preparation of infectious diarrhea related virus detection products, a specific detection kit and a specific detection method. According to the present invention, the multiple detection of three common diarrhea viruses can be achieved, the detection can be completed in a short time, the amplification product can be rapidly and accurately identified, the color development can be performed, the detection sensitivity and the specificity can be improved, the large-scale instrument is not required, and the cost is low.

The present invention discloses a method, system, device and medium for identifying and classifying lesions of endoscopic mucosal images. The method includes: reading a video signal output by an endoscopic device to obtain a mucosal image; calculating multiple quality feature values ​​of the mucosal image and determining the image quality of the mucosal image accordingly; predicting normal mucosal areas, unclear mucosal areas, abnormal mucosal areas and abnormal mucosal area classification results in mucosal images that meet preset image quality standards; and fusing the predicted results onto an image to generate an identification classification map. The present invention improves the accuracy of abnormal mucosal areas and classification by screening out unclear images and then identifying clear areas in clear images, thereby improving the accuracy and reference of the lesion identification and classification method of endoscopic mucosal images for assistance; through classification prediction, the analysis of abnormal mucosal areas is realized, and classification based on clear image areas makes the classification accurate.