150results about How to "Accurate typing" patented technology

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention provides a method for carrying out large-scale gene typing based on an n SLAF-seq (Specific-Locus Amplified Fragment Sequencing) technology. Complexity of the genome is reduced by utilizing the SLAF-seq technology, and genetic typing is carried out on large-scale products. High-throughput sequencing is carried out on the genome, marker-developing, genetic map drawing and whole genome association analyzing are carried out on the samples by utilizing the technology. Compared with the conventional method, the large-scale genetic typing method disclosed by the invention has the advantages that the throughput is greatly improved, and the cost is greatly reduced. The method is mainly applied to marker-developing, genetic map drawing and whole genome association analyzing.

The invention relates to a preparation method for probes related to breast cancer molecular markers and to preparation of a breast cancer fluorescence in situ hybridization detection kit by using the probes. The breast cancer fluorescence in situ hybridization detection kit can be prepared from HER2, TOP2A and AGTR1 gene probes prepared by using the method provided in the invention, human chromosome 17 counting probe, hybridization buffer, unlabelled competitive DNA and DAPI counter strain; application of the kit enables the detection rate of breast cancers to be substantially improved and type sorting of breast cancers to be more accurate and provides guidance to formulation of an individual therapeutic schedule and selection of proper therapeutic drugs, thereby lowering down mortality, reducing recurrence risk and achieving the goal of optimizing the effect of diagnosis and treatment.

The invention provides a human papillomavirus HPV DNA fragment, a mix primer for human papillomavirus HPV DNA detection and/ or parting and application thereof. The mixed primer can specifically amplify to obtain one or a plurality of DNA sequences from HPV6, HPV 11, HPV 16, HPV 18, HPV 26, HPV 31, HPV 33, HPV 35, HPV 39, HPV 40, HPV 42, HPV 43, HPV 44, HPV45, HPV 51, HPV 52, HPV 53, HPV 54, HPV 55, HPV 56, HPV 58, HPV 59, HPV 61, HPV 66, HPV 68, HPV 70, HPV 72, HPV 73, HPV 81, HPV 82 and HPV 83, can be used for the human papillomavirus HPV DNA detection and/ or parting and prepared into a PCR kit. The invention can detect common types of HPV at a time, greatly improve the HPV detection efficiency, overcome the defect of missed detection of latent infection by a serology and IHC detection method, realize early diagnosis of HPV diseases, and is suitable for clinical HPV gene detection and parting and guides prevention and cure of cervical carcinoma.

The invention belongs to the field of forensic medicine, and in particular relates to a medicolegal composite assay kit based on twenty triallelic SNP (single nucleotide polymorphism) genetic markers. The kit consists of a composite amplification primer mixture, a multiple single-base extension reaction primer mixture, an allelic typing reference material mixture, a composite amplification reaction mixed liquor and a single-base extension reaction mixed liquor, which are packed in a separate way. The kit can quickly and exactly obtain the typing of twenty triallelic SNP (single nucleotide polymorphism) genetic markers of a biologic assay material at one time to ensure the individual source of the assay material and provide a new technical means for the personal identification by a composite amplification reaction and a multiple single-base extension reaction in a single pipe and a capillary electrophoresis platform generally used in a medicolegal heredity lab. Therefore, the kit has good application prospect in the field of forensic medicine.

The invention relates to the field of gene mutation detection, and specifically relates to primers and a probe used for detecting EGFR gene mutation, a kit thereof, and an application method of the kit. With the kit provided by the invention, a sample containing 0.1-1% of EGFR gene mutation DNA can be detected; and accurate genotyping can be carried out upon a sample containing 10% of EGFR No. 19 exon gene mutation DNA through sequencing. The sensitivity of the kit provided by the invention is high, wherein a minimum detection limit is 5-copy gene. With the kit, qualitative detection can be carried out upon EGFR gene mutation. Different sensitivity reference ranges can be provided for assisting clinical determinations of mutation amount volumes, such that final treatment programs can be provided according to different mutations.

The invention discloses wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof in the field of wheat molecular breeding. Three KASP markers (AX-111578083, AX-111624595 and AX-110772653) in the third homologous group of wheat, developed on the basis of an SNP marker and a flanking sequence of the SNP marker, are capable of accurately typing preharvest sprouting resistance candidate loci and predicting the preharvest sprouting resistance of the wheat, thereby more conveniently identifying and screening preharvest sprouting resistance materials under laboratory conditions. By using a molecular marker-assisted selective breeding technique, the influences of environmental factors and personal errors on phenotype identification can be effectively avoided, and the molecular marker-assisted selective breeding technique can be combined with phenotype identification for use. According to the three KASP markers, strongly selected loci can be provided, the accuracy of wheat preharvest sprouting resistance breeding material selection is improved, and the target of wheat preharvest sprouting resistance molecular marker-assisted selective breeding is achieved.

The invention provides a primer set, application, a product and a method for detecting SNP sites related to drug metabolism in children, and relates to the field of biotechnology. The primer set of the SNP sites related to drug metabolism in children provided by the invention can be used to detect the metabolism capacity of thermoanalgesics, metabolism capacity of respiratory system drugs, metabolism capacity of neurological and psychiatric drugs, metabolism capacity of cardiovascular and cerebrovascular drugs, metabolism capacity of anti-infective drugs, metabolism capacity of endocrine drugs, metabolism capacity of digestion system drugs, metabolism capacity of anesthetic drugs and metabolism capacity of chemotherapy and immunosuppressant. Specific detection of site mutation related to common drug metabolism in children can be realized by the primer set, the accuracy is high, the detection cycle can be tremendously shortened, and the detection cost is reduced at the same time. Moreover, the needs of a drug metabolism can be predicted by the detection results, the metabolism capacity of a variety of drugs can also be comprehensively evaluated, and scientific reference is providedfor the individualized medication of children.

The invention discloses nucleic acid and a kit for detecting G1165C polymorphism of the human ADRB1 gene, and also establishes a method for detecting the G1165C polymorphism of the human ADRB1 gene, which is strong in specificity, high in sensitivity, high in degree of accuracy, and easy to operate. The kit for detecting the G1165C polymorphism of the human ADRB1 gene is applicable to the selection for various clinical samples, and has the remarkable advantages of strong specificity, high sensitivity, short experimental period, operation simplicity, safety and non-toxicity, low cost, and the like. The detection method provided by the invention adopts the completely closed tube operation, and is easy, convenient and rapid to operate, the detection result is obtained by directly directing fluorescence signal values during the PCR process, the PCR aftertreatment or electrophoresis detection is not needed, the large possibility of pollution and false positive property during the conventional PCR technology are overcome, the difficulty of non-specific amplification can be effectively avoided, and the detection method is suitable for detection samples in large batches.

The invention belongs to the field of biotechnical detection, and particularly relates to a bovine, goat, porcine and canine brucella typing fluorescent PCR (polymerase chain reaction) detection reagent kit and preparation and application thereof. The bovine, goat, porcine and canine brucella typing fluorescent PCR reagent kit contains bovine brucella detection primers and probes, goat brucella detection primers and probes, porcine brucella detection primers and probes and canine brucella detection primers and probes. The bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit, the preparation and the application have the advantages that the bovine, goat, porcine and canine brucella typing fluorescent PCR detection reagent kit is high in detection sensitivity, the lowest detection limit is 1*10<3> copy/ml, and the accuracy and the positive rate can reach 100%.

The invention relates to the technical field of gene polymorphism detection, in particular to a detection method of yak FOXO1 gene single nucleotide polymorphism and a kit thereof. The detection method comprises the following steps: 1) preparing a yak FOXO1 gene amplification product; 2) synthesizing a high and low temperature interior label; 3) preparing an HRM-PCR (High Resolution Melt-Polymerase Chain Reaction) amplification product; 4) collecting a fluorescence signal. Compared with an HRM method used for detecting polymorphic sites in the prior art, a gene probe designed by the detection method is more accurate and flexible in positioning, is accurate in parting, does not need PCR post-processing, has high working efficiency and good repeatability and is low in sample quality and quantity requirements, especially, the concentration requirement of a DNA template can be lowered to 0.1ng/mu L, and time and labor are saved when a sample size is large. Meanwhile, the detection kit has the advantages of high sensitiveness, high detection speed and good stability.

The invention discloses a cancer subtyping method and a device based on RNA targeted sequencing and machine learning. According to the method, through RNA targeted sequencing technology, a target genearea is concentrated efficiently. Through steps of inverse transcription, database establishing and sequencing, second-generation sequencing data of the target area are obtained. Furthermore a randomforest algorithm is used for training on a TCGA dataset for obtaining a tumor typing predicting model, thereby accurately performing cancer subtyping. The method according to the invention can obtaina model for realizing lung cancer and renal cell carcinoma typing with high accuracy. The method according to the invention can reduce typing cost. Furthermore the typing speed, precision and the typing result accuracy are better than that of a traditional method.

The invention discloses a whole set reagent and a method for detecting a human papilloma virus. The whole set reagent for detecting the human papilloma virus disclosed by the invention consists of 621probes. The sequences of the 621 probes are sequences 1 to 621 in the sequence listing, and 1 to 15 positions and 94 to 108 positions of all sequences 1 to 621 in the sequence listing are the same and can be used for amplifying the 621 probes; the 5' end of each probe can be labeled with biotin. The whole set reagent provided by the invention can distinguish different types during capture of human papilloma virus genomes and sequencing, can also determine whether the DNA is integrated into human genomes or not, can also determine the copy number of the human papilloma virus, and have the characteristics of accurate typing, capability of obtaining integrated information and copy number, high sensitivity, and the like, thus meeting the development trend of precision medicine, and having great significance.

The invention discloses a folate metabolism related gene MTHFR heritable variation detection kit.The kit is used for identifying rs1801133 genotypes and contains that 1, forward and reverse primers: the forward primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.1, and the reverse primer is a nucleotide strand or its complementary strand of a sequence shown by SEQ ID NO.2; 2, a PCR reaction: 2 x HRM PreMix.In addition, the invention further discloses application of the kit.The detection kit adopts polymerase chain reaction, a reaction system contains specific forward and reverse primers, and the genotypes are judged through real-time fluorescence quantification detection.The folate metabolism related gene MTHFR heritable variation detection kit has the advantages of being low in cost, good in specificity, high in sensitivity, simple and quick to operate and accurate in classification, making results easily interpreted and the like, can be used for clinic detection of folate metabolism related gene MTHFR heritable variation and assists a doctor to select an appropriate dose of folic acid.

The invention belongs to the technical field of biology, and relates to a multiplex amplification system for rapidly mutating Y-chromosome short tandem repeats, a kit and an application. The multiplexamplification system comprises 16 pairs of primers, and can be used for amplifying 16 STR loci: DYS630, DYS464, DYF403S1b, DYF399S1, DYS518, DYF403S1a, DYS527, DYS713, DYS612, DYS626, DYS627, DYS526,DYF404S1, DYF387S1, DYS449, and DYS547. Compared with prior art, the system is advantageous in that: 1. the system contains 16 human male Y-chromosome high-mutation rate STR, and the system is uniqueand novel with a large amount of information content and good compatibility; 2. the system has wide application range, good directivity, and high precision, and the system is suitable for legal medical expert DNA analysis in material evidence cases which relate to all cells of male; wherein biological testing materials which contain mal cells, such as bloodstain, seminal stain, saliva, hair, nail, cartilage and other human tissues, can be identified. 3. the system has good system specificity and good stability, multiple repeat checking is not needed, non-specific amplification products are not generated, and signal intensity is stable; 4. the system has high sensitivity, and DNA template amount which is low to 15pg can be accurately classified.

The invention discloses an SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and an application thereof. A mark primer is at least one selected from Na12E02, KBRH138G23 or BrSS15. The SSR molecular mark provided by the invention can be applied in rapid identification and detection of the self-incompatibility of brassica campestris ssp. chinensis Makino, increase breeding efficiency and accelerate a breeding process.

The invention relates to a microfluidic-technology-based CTC protein typing kit. The kit comprises: (1) capture antibody compounds aiming at different CTCs marker protein, each capture antibody compound being made from a capture antibody in specific binding to corresponding marker protein and an aptamer, wherein the capture antibody and aptamer are connected through a cuttable material which is deoxyribonucleotide double strands having a palindrome structure and two or more than two restriction enzyme sites; and (2) a microfluidic chip, the surface of which is coupled to a cell capture assembly. The cell capture assembly made from a microcolumn is fixed at the surface of the microfluidic chip, and a specific conjugate capable of bonding to the aptamer modifies one end of the microcolumn. Through the microfluidic technology, efficient and fast automatic detection and typing of CTCs is achieved, the kit is simple to operate, false-negative and false-positive results due to man-made operational factors are reduced, and the sensitivity and accuracy of detection are improved.

The invention relates to the technical field of cell biology detection, and particularly relates to an accurate detection kit for typing of a tumor immune cell subset. The invention provides markers for single-cell immune cell typing detection and an antibody combination thereof. The combination covers 42 markers and metal pre-labeled antibodies thereof for immune basic detection, lymphoid cell extension detection and myeloid cell extension detection. The antibody combination and a mass spectrum flow cytometry are used for typing detection of immune cells, various detection requirements can bemet, accurate detection of complex tumor immune cell heterogeneity and rapid and accurate typing of the immune cell subset are achieved, single-staining control and calculation compensation of all channels are not needed, samples are effectively saved, and the detection efficiency is improved.

The invention discloses a fluorescent composite amplification system, a kit and application of the kit. The fluorescent composite amplification system comprises multiple pairs of specific primers, hasa nucleotide sequence shown in SEQ ID NO.1-71 and is used for preparing the kit, and the prepared kit is applied to DNA analysis and source analysis by legal medical experts. The Y-SNP contains multiple haplogroups and has high discrimination for male groups, and the kit is unique, novel, large in information amount and good in compatibility.