Fluorescent automated detection primer and kit of pathogenic sites of Arachnomelia syndrome (AS) in Simmental cattle

Abstract

The invention relates to a fluorescent automated detection and analysis method of pathogenic sites of Arachnomelia syndrome (AS) in Simmental cattle. The nucleotide sequence of a primer for PCR (polymerase chain reaction) amplification is as follows: 5'-CTGAGTCTCCTCTTCTGTTTTCA-3',5'-GTTGGCATCTGAGTCCAGGT-3'. By using the fluorescent labeling primer PCR amplification and electrophoresis typing technology, the kit for typing pathogenic sites of AS in Simmental cattle has the advantages of stable result and low cost, and is accurate and reliable, thereby accurately typing the pathogenic sites of AS in Simmental cattle.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting a Simmental spider leg syndrome (AS) pathogenic site fluorescently labeled primer PCR detection method. Background technique

[0002] The reason why livestock genetic defects mostly show a recessive inheritance pattern may have something to do with the production methods of livestock, especially dairy cows. The application of artificial insemination (AI) and semen cryopreservation techniques (Semen Cryopreservation Techniques) in the production and breeding of dairy cows has laid a solid foundation for the establishment of the artificial insemination breeding system (AI breeding system) of dairy cows. Because AI technology can undertake more mating tasks for female animals, bulls can be widely used in groups to obtain a large number of offspring, allowing high-yielding genes to spread rapidly among groups. Semen cryopreservation technology makes ex...

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