184results about How to "Enable high-throughput detection" patented technology

The invention relates to inspection and quarantine systems for biomedicine, agriculture, food hygiene and the like and particularly relates to an absolute quantification type digital nucleic acid analytic system based on an efficient liquid drop microreactor. The absolute quantification type digital nucleic acid analytic system is mainly used for detecting the molecular structure of nucleic acid so as to analyze attributes of organisms and belongs to the fields of biological and medical detection. The absolute quantification type digital nucleic acid analytic system comprises a confocal fluorescence image collecting system, a micro-fluidic chip, a temperature circulating heating system, an electric loading platform, an automatic sample feeding device, a controller and a computer. The formation and PCR amplification of micro-droplets are finished by virtue of the same micro-fluidic chip, namely the micro-fluidic chip has a droplet formation function and a PCR amplification (microreactor) function. According to the absolute quantification type digital nucleic acid analytic system, the complexity of the absolute quantification type nucleic acid analysis process is reduced, the nucleic acid analysis process is finished in a one-button manner, and culture and bacteria-proliferating processes are omitted, so that the rapid detection is realized, the sensitivity of the absolute quantification type nucleic acid detection is greatly improved, meanwhile, the sample consumption of the absolute quantification type nucleic acid analysis is substantially reduced, and the high throughput detection is realized.

The invention provides a primer, a probe, a reagent kit and a method for detecting mutation of BRAF gene V600E on the basis of real-time fluorescent quantitative PCR (polymerase chain reaction) technical platform. The primer, the probe, the reagent kit and the method are quick and low in cost and have clinic detection popularization value.

The invention relates to a method for measuring antigen protein in serum by chemiluminescence protein chips and a kit, which belongs to the medical detection technology. The method is characterized in that a chemiluminescence technology, a standard curve and a protein chip technology are combined for performing quantitative determination of certain antigen protein substance in serum, the high specialty and sensitivity of the chemiluminescence technology and the standard curve are employed for realizing the quantitative determination; the protein chip technology can realize simultaneous detection and analysis of several samples, so that the detection time consumption of a whole set ofe sample is greatly reduced, and the method has the characteristics of reliability, accuracy and low cost; and the method is suitable for general analysis of common antigen protein level in serum and quantitative analysis of great disease antigen marks, and the method is an medical auxiliary detection method having great meaning. The invention also provides a matched kit based on the method.

The invention discloses an EGFR (epidermal growth factor receptor) gene mutation site detection kit which detects EGFR gene mutation sites by combining the ARMS (amplification refractory mutation system) specific mutant enrichment technology with the Taqman-MGB (minor groove binder) probe specific detection technology. The kit comprises ARMS primer pairs and corresponding Taqman-MGB probes, and the ARMS primer pairs and the corresponding Taqman-MGB probes are used for detecting the EGFR gene mutation sites. The EGFR gene mutation site detection kit is high in sensitivity, capable of detecting multiple samples simultaneously and low in cost, provides guidance for pharmacy of clinicians, realizes individualized treatment of patients suffering from tumors and is capable of reducing treatment risk and burden of the patients and wide in application prospect.

The invention discloses a matrix-assisted excitation spectrum detection system adopting plasma surface sample injection. The matrix-assisted excitation spectrum detection system adopting plasma surface sample injection comprises a carrier gas system, a microwave source system, a sample injection system, a microwave induced plasma excitation source, a detection system for carrying out collection, transmission, beam splitting and detection on the detection light emitted by the microwave induced plasma excitation source, and a data processing system for carrying out analysis processing on the output signal of the detection signal, wherein the carrier gas system is connected with the gas discharge tube inlet of the microwave induced plasma excitation source, the signal input end of the data processing system is connected with the signal output end of the detection system; the microwave induced plasma excitation source is connected with the microwave source system through a microwave transmission line. According to the matrix-assisted excitation spectrum detection system adopting plasma surface sample injection, disclosed by the invention, elemental analysis is carried out in a manner of surface sample injection; the system is miniaturized in composition, good in production stability, high in sensitivity, simple in device, simple and convenient to operate, low in sample consumption, high in analysis speed, low in energy consumption, and low in gas consumption.

The invention provides a primer, probe, fluorescent PCR kit and method for detecting nucleotide polymorphisms of two sites of c.665 and c.1286 in an MTHFR (Methylene Tetrahydrofolate Reductase) gene. The nucleotide types of the two polymorphic sites of c.665 and c.1286 in the MTHFR gene are detected on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology platform according to the principle of 'allelic specific PCR'.

The invention relates to a composition and method for detecting drug resistance of staphylococcus aureus, in particular to a method for using DHPLC for carrying out detection on a multiplex PCR amplification product after multiplex PCR amplification. The determination of the drug resistance of the staphylococcus aureus can be easy, convenient, rapid, and high in sensitivity and specificity through the composition and method for detecting the drug resistance of the staphylococcus aureus.

The invention provides primers, a probe, a fluorescent PCR kit and a detection method for detecting a human HLA-B*1301 allele. According to the theory of 'allele specific PCR', a human leucocyte antigen HLA-B*1301 allele can be detected on a real-time fluorescent quantitative PCR technical platform.

The invention provides a complete premixed multiple primer-specific PCR detection kit and method for detecting nucleotide polymorphisms at three sites of c.677 and c.1298 of the folic acid metabolic pathway and c.1298 of the MTRR gene. "Allele-specific PCR" principle, design multiple primer-specific PCR, realize two-tube reaction on the real-time fluorescent quantitative PCR technology platform, and simultaneously detect three folic acid metabolic pathway MTHFR genes c.677 and c.1298 and MTRR gene c.66 The nucleotide type of the site. In addition, modified Taq DNA polymerase and disaccharide PCR protective agent are added to the reaction system to increase the stability of the reaction system and realize the long-term stability of the pre-mixed detection system.

The invention relates to a high throughput test method for a tomato bacterial disease, and more particularly relates to a high throughput test method which can utilize a locking probe to carry out reverse dot blot hybridization on tomato bacterial wilt original bacteria, tomato ulcer pathogenic bacteria and a tomato spot disease, and belongs to the crop preventing and curing disease and exit and entry plant quarantine range. The high throughput test method provided by the invention is based on a rolling circle amplification technology of the locking probe, a pair of universal primers are utilized for amplifying multiple pathogens, then, the high throughput test method and a reverse dot blot are combined together, a result is judged through a chromogenic reaction, the testing time of the traditional tomato bacterial disease is shortened, the specificity and the sensitivity of the detection are guaranteed, and the high throughput test is realized. According to the method, the testing cycle of the traditional pathogenic bacteria can be shortened, the cargo clearance can be increased, the intercept and capture relevance ratio of epidemic situations of imported bulk farm-product is improved, the pest invasion capacity is improved, the cargo clearance of cargoes imported and exported at ports in China is accelerated, and the enterprise cost is reduced and the high throughput test method has the important meanings.

The invention discloses a bridge-PCR-based high-flux chip detection method for DNA hydroxymethylation. . The detection method comprises: firstly fixing different forward primers for amplyfying DNA hydroxymethylation sites to a chip or a microballon; secondly using beta-glucanotransferase to glycosylating all 5hmC on DNA, then using MspI enzyme for enzyme digestion, wherein hydroxymethylated CCGG cannot be cleaved and non-hydroxymethylated CCGG can be cleaved; thirdly hybridizing genome DNA subjected to enzyme digestion with the chip, and performing bridge PCR, wherein the hydroxymethylated CCGG sites can have amplification because the hydroxymethylated CCGG cannot be cleaved, and conversely the non-hydroxymethylated CCGG sites cannot have amplification because the non-hydroxymethylated CCGG can be cleaved; and finally, according to presence or absence of fluorescence at different matrix points of the chip, determining whether CCGC at different positions of the genome DNA is subjected to hydroxymethylization. The beneficial effects comprises: parallelism detection on different DNA hydroxymethylization sites is realized, and the detection results have the characteristics of high flux and high sensitivity.

The invention provides a detection kit. The kit adopts UGT1A1*60(-3279T/G), UGT1A1*93(-3156G/A), UGT1A1*28(-536/7) and UGT1A1*6(211G/A) four loci as the detection objects, and takes molecular beacon probe fluorescent quantitative PCR as the basis to carry out genotyping detection. The kit provided by the invention can detect four loci at one time, greatly saves cost, and provides a reliable method for clinical diagnosis and scientific research of relevant fields.

The invention relates to the field of molecular detection, and in particular relates to a primer pair, a kit and a detection method for judging abamectin resistance of tetranychus urticae. The primer pair comprises (1) upstream primers for detecting resistant and susceptible populations, which are RF:5'-AAGCTGTTGACATTTGGACGA-3' and SF:5'-AAGCTGTTGACATTTGGACGG-3', and (2) a common downstream primer which is paired with the upstream primers for use. According to the primer pair, the kit and the detection method, the resistance or susceptibility of tetranychus urticae to abamectin is judged by detecting GluCl (Glutamate-gated chloride channel) gene mutation in tetranychus urticae. The primer pair, the kit and the detection method have the advantages of high speed, effectiveness, high sensitivity, capability of realizing high-flux detection of a large number of samples and the like, and have the effects of monitoring the resistance gene frequency and resistance development trend of the field population of tetranychus urticae to abamectin, timely adjusting a prevention and control strategy for tetranychus urticae, guiding rational drug use and delaying the resistance development.

The invention relates to a device, a system and a method for real-time fluorescence quantitative nucleic acid amplification detection. The device comprises a micro-fluidic unit, a sample injection unit, a temperature control unit and a driving unit; the micro-fluidic unit is provided with one or more parallel annular flow channels; the annular flow channels are provided with a liquid inlet and a liquid outlet; the sample injection unit is arranged at the liquid inlet of the annular flow channels and is used for injecting samples into the annular flow channels; the temperature control unit is matched with the micro-fluidic unit; the driving unit is matched with the micro-fluidic unit and is used for driving a micro-fluid in the annular flow channels to move; at least one section of the annular flow channels is made of a transparent material, so that fluorescence detection is facilitated; and different flow sections of the annular flow channel have different preset temperatures through configuration of the temperature control unit, and through moving in the flow sections with different temperatures in the annular flow channels, the micro-fluid realizes temperature change operation. The invention simplifies nucleic acid amplification and detection operation, is flexible to control, reduces detection time and material cost, and improves detection efficiency.

The invention provides a primer, a probe, a fluorescence PCR kit and a method for detecting nucleotide polymorphism at *6-type and *28-type loci of the UGT1A1 gene. According to the allele specific PCR principle, the nucleotide type at *6-type and *28-type polymorphism loci of the methylenetetrahydrofolate reductase UGT1A1 gene is detected on a real-time fluorescence quantitative PCR technical platform.

Disclosed is a transcription factor protein detection method through exonuclease III digestion FRET-dsDNA micro array chips, wherein the expression and activation levels of the transcription factor are analyzed through the following steps: (1) preparing FRET-dsDNA micro array chips, (2) reacting the transcription factor with FRET-dsDNA micro array chips, (2) reacting the exonuclease III with FRET-dsDNA micro array chips, (1) proceeding detection and analysis to the FRET-dsDNA micro array chips.

The invention relates to a method for quantitatively detecting a SiO2-FOtargeted nano-drug carrier on the basis of direct competitionfluorescence immunoassay. SiO2-FO nano particles are subjected to quantitative detection and analysis through combination with specificity of antigen and antibody reactions and sensitivity of fluorescence. The method comprises steps as follows: a SiO2-FOenvelope antigen and animmunogen are prepared, the immunogen is injected into a body of an animal, a high-specificity antibody is obtained and marked with FITC(fluorescein isothiocyanate), a fluorescent antibody is prepared, and content of SiO2-FO is accurately measured through established direct competitionfluorescence immunoassay. The detection method is simple to operate, sensitivity and specificity are high, and high-throughput detection can be realized.