4results about How to "Enable high-throughput detection" patented technology

The application discloses a kind of molecular markers for Camellia genus Camellia reticulata group plant identification, which is composed of 3 Camellia reticulata specific DNA sequences.The application also provides a Camellia reticulata identification method based on the molecular markers, and the operation process is as follows: total DNA extraction is carried out on the sample to be tested, then high-throughput sequencing is carried out, the sequencing reads obtained are compared with the molecular markers, and if the sequencing reads can completely cover the molecular markers, it can be determined that the sample to be tested belongs to Camellia reticulata.

The invention discloses a PEDV (Porcine Epidemic Diarrhea Virus) sIgA detection reagent, a PEDV sIgA detection method and application. The reagent comprises a first probe and a second probe. The first probe comprises a first binding element and a first fluorescent reporter molecule which are coupled with each other, and the first binding element comprises a PEDV S2 protein capable of being combined with a variable region of PEDV sIgA or a stabilizing mutant of a functional fragment of the PEDV S2 protein. The second probe comprises a second binding element and a second fluorescence reporter molecule which are coupled with each other, and the second binding element comprises an anti-porcine IgA antibody capable of being bound with a PEDV sIgA constant region. The first fluorescent reporter molecule and the second fluorescent reporter molecule form a fluorescence resonance energy transfer (FRET) pair. Compared with the prior art, the detection reagent provided by the invention can realize rapid, homogeneous and high-throughput quantitative detection of PEDV sIgA, and the detection sensitivity, the specificity and the signal-to-noise ratio of the detection reagent are greatly improved.

The application discloses a bio-sample quantitative detection method based on an optical disc and belongs to the technical field of biosensors. The method comprises the following steps: for a mixed system to-be-detected solution, based on the inhibition or promotion effect of the solution on an insoluble precipitate solution, the solution is reacted with the insoluble precipitate, and a suspension of the remaining insoluble precipitate after the reaction is prepared; after the surface of an optical disc full of data is subjected to an activation treatment, the insoluble precipitate suspension corresponding to the mixed system to-be-detected solution or a single system to-be-detected solution is added dropwise on the optical disc surface at a specified radius to form a sample drop point; after the water in the sample drop point is completely evaporated, the data in the optical disc is read, data reading errors are obtained, and corresponding LDC counts are calculated; and the LDC counts are substituted into a pre-calibrated standard curve or a relational expression to obtain the concentration of the to-be-detected solution. The application can improve the efficiency and universality of quantitative detection of substances in a solution and reduce detection cost and detection complexity.

The application discloses a rare earth up-conversion red-green-blue fluorescent coding microsphere, adopts a modified olefin-norbornene copolymer as a matrix material, and adopts red, blue or green rare earth up-conversion luminescent nanocrystals as a fluorescent coding material. The application further discloses a preparation method of the rare earth up-conversion red-green-blue fluorescent coding microsphere. The modified olefin-norbornene copolymer is blended with the up-conversion luminescent nanocrystals to obtain a uniform blend, and the blend is hot-pressed into a rod-shaped core layer which is inserted into a cladding material to form a preform rod, and then the preform rod is hot-drawn into a fiber, and then heat treatment is carried out to obtain a polymer fluorescent microsphere with a coding function. The method can control the fluorescent type and intensity of the fluorescent coding microsphere and the particle size of the microsphere according to requirements, the preparation process is simple, the method is suitable for large-scale production, the fluorescent performance is stable, and the method can be used for detecting cells, proteins or other molecules in biological samples.