682 results about "Mixed systems" patented technology

This invention advances power grid operational planning by introducing a mid-term scheduling framework for integrated hydro-wind-solar systems that accounts for heterogeneous daily load profiles across multiple receiving-end power grids. The proposed approach utilizes an adaptive variable-step search algorithm to segment loads into peak, flat, and valley intervals. By synthesizing five key metrics, including mean daily load, daily load factor, peak-valley differential ratio, load rates during peak / valley periods, and timing of peak / valley occurrences, the method accurately captures region-specific load patterns and peak-shaving demands. This enables a refined reconstruction of load profiles of receiving-end power grids. A nested multi-temporal scheduling model that couples medium- and short-term horizons to simultaneously maximize total energy production and minimize transmission imbalances among power grids. The model is addressed by using the mixed-integer linear programming (MILP) to obtain medium- and short-term generation schedules and power transmission schedules.

The invention discloses a mixed surfactant system surface tension prediction method and system based on machine learning. Measuring system surface tension data of the mixed surfactant at different temperatures through experiments; secondly, carrying out theoretical correction and data enhancement on the original data by utilizing a Szyszkowski equation; then constructing a multi-dimensional feature space; a nested K-fold cross validation strategy is adopted; the finally established XGB prediction model is excellent in performance on a test set of an enhanced data set, the determination coefficient R2 reaches 0.9994, and the mean square error MSE is 0.0713. The method is particularly suitable for a binary mixed system containing a nonionic polyether surfactant, and not only can accurately predict the surface tension value, but also can determine key parameters such as critical micelle concentration (CMC) and the like. An efficient and reliable technical means is provided for rapid screening and optimization of a surfactant formula, and the method has wide application prospects in the fields of daily chemicals, petroleum, pharmacy and the like.

The invention relates to a high-stability bronze powder pigment and a preparation method and application thereof.The preparation method comprises the following steps that copper-zinc alloy coarse powder, stearic acid and a dissolved compound passivator are taken and subjected to ball milling in the nitrogen atmosphere, and a ball milling product is screened, washed and subjected to suction filtration to obtain an amino-functionalized passivated base material; the preparation method comprises the following steps: dispersing SiO2 in anhydrous isopropanol to obtain a reaction system, adding deionized water and a catalyst, dissolving tetraethoxysilane and phenyltriethoxysilane in anhydrous isopropanol, slowly dropwise adding, carrying out a heat preservation reaction, and carrying out suction filtration and washing to obtain a phenyl modified SiO2 coated intermediate; the preparation method comprises the following steps: dispersing a compound stabilizer in anhydrous isopropanol to obtain a mixed system, heating and stirring, dissolving the compound stabilizer in anhydrous isopropanol, dropwise adding into the mixed system, reacting, centrifuging, washing, and drying in vacuum to obtain the bronze powder pigment. The bronze powder pigment disclosed by the invention has excellent oxidation resistance, chemical corrosion resistance and long-term storage stability, and can keep the natural color gloss of metal to the greatest extent.

The invention relates to the technical field of hydrogel materials, and discloses a preparation method of self-growing hydrogel based on spatial morphology gradient change. The method comprises the following steps: constructing a hydrogel self-growth reaction environment comprising a self-growth initiator layer and a space confinement layer; covering the space confinement layer with a self-growing monomer aqueous solution, and forming concentration gradient distribution through diffusion to obtain a self-growing reaction mixing system; culturing the self-growth reaction mixed system, and forming the self-growth hydrogel with spatial morphology gradient change through a self-growth reaction of the self-growth monomer based on concentration gradient difference and the initiation effect of a free radical initiator; by selectively degrading alginate single-network hydrogel in a target mixed system, homogeneous self-growing hydrogel with spatial morphology gradient change is obtained. Based on the method, external equipment or a complex process is not needed, and the hydrogel can form a structure with spatial morphology gradient change in a self-growth mode under a constant-temperature condition.

A temperature regulation system for samples in a microplate includes a housing defining an interior volume and first and second airflow ports defined by a first and second exterior surfaces of the housing. The system includes an electromagnetic mixing system that has a base plate defining a plurality of openings, and a plurality of electromagnets. Each electromagnet defines an axis that extends substantially vertically from the base plate. The base plate is disposed in the interior volume and each of the plurality of electromagnets extend toward the first airflow port. A fan is disposed in the interior volume substantially below the base plate. Activation of the fan draws air into the first or second airflow port, substantially parallel to each axis of the plurality of electromagnets, through the plurality of openings, and out of the other of the second and first airflow port.

An assembly for delivery of mixed chemical for use in a vehicle wash system includes a chemical delivery device for dispensing chemical having a piston with a one-way valve and a pneumatic drive mechanism; and a fluid delivery manifold configured to deliver motive fluid for mixing with the dispensed chemical having an integrated valve with a valve plunger therein configured to be pneumatically actuated. During a dispensing operation, chemical and fluid are dispensed and mixed at a mixing site of the assembly. During a resetting stroke of the chemical delivery device, the drive shaft and the piston are pneumatically retracted away from the outlet to a retracted position, and the one-way valve is in an open state and permits passage of chemical through the one-way valve for dispensing from the chemical chamber in a subsequent dispensing operation.

The invention relates to the technical field of preparation of high-purity boron oxide, and discloses a purification method of high-purity boron oxide and a device thereof, and the purification method comprises the following steps: step 1, mixing crude boron oxide with a liquid metal alloy, and dissolving metal impurities through gradient temperature control; 2, applying ultrasonic treatment to the mixed system, introducing a vortex field, and stripping impurities on the surface of the boron oxide; 3, a liquid metal phase and a boron oxide solid phase are separated through cooperation of a magnetic field and a pulse high-voltage electrostatic field; 4, non-metal ion impurities are removed through a gradient porous ceramic adsorption bed; and step 5, melting boron oxide in vacuum and carrying out gradient cooling crystallization. By adopting the technical scheme of a Ga-I n-Bi-Er quaternary liquid alloy system, the technical effect of selective targeted removal of metal impurities is achieved, and compared with a liquid metal system depending on single phase solubility difference in the prior art, the defect that efficient chelating and dynamic settling separation of the impurities cannot be achieved at the same time is overcome.

The invention relates to the technical field of coatings, and discloses an anti-falling environment-friendly wall paint and a preparation method thereof.The wall paint is composed of, by weight, 30-40 parts of composite resin, 10-20 parts of nanoscale filler, 10-20 parts of auxiliaries and 10-20 parts of additives. 10 to 14 parts of a nano-scale filling material; 5-10 parts of an auxiliary agent; the preparation method comprises the following steps: preparing the composite resin, the nano-scale filler, the auxiliary agent and the additive according to the weight parts of the formula, putting the composite resin and the nano-scale filler into a high-speed dispersion kettle, setting stirring conditions, adding the auxiliary agent twice, adding the additive again, adjusting the stirring conditions, forming a mixed system, adding the thickening agent and the deionized water to adjust the viscosity, forming a mixed material, and uniformly stirring. The preparation method comprises the following steps: adding the raw materials into a stirrer for stirring, adjusting the acid-base balance of the materials, carrying out curing treatment, carrying out defoaming operation on the cured materials under a vacuum condition for 15-20 minutes, completing the preparation of the wall paint, and finally detecting that the wall paint has the advantages of low VOC emission, no formaldehyde, no benzene, excellent adhesive force and strong weather resistance.

The invention relates to the technical field of traditional Chinese medicine nano preparations, in particular to traditional Chinese medicine polysaccharide radiotherapy sensitization nanoparticles and a preparation method thereof.The preparation method comprises the following steps that 1, a chitosan-based carrier and traditional Chinese medicine polysaccharide are dissolved in a buffer solution, and the chitosan-based carrier is selected from chitosan, carboxymethyl chitosan or quaternized chitosan; step 2, putting the chitosan-based carrier solution into a reaction container, and dropwise adding glacial acetic acid to adjust the pH value of the system; 3, a cross-linking agent solution is dropwise added at a constant speed, a cross-linking agent is a multivalent anion / cation compound, and stirring is performed to form a core-shell structure; step 4, dropwise adding a traditional Chinese medicine polysaccharide solution into a mixed system in the step 3, and continuously stirring; step 5, filtering and granulating the obtained solution to obtain nanoparticles; according to the traditional Chinese medicine polysaccharide radiotherapy sensitization nanoparticles and the preparation method thereof, not only is radiotherapy sensitization achieved, but also the immunoregulation function is achieved, and the synergistic interaction of radiotherapy and immunotherapy is achieved by activating dendritic cells and T cells.

The object of the invention is a mixing system (1) comprising at least a boiler (2) and a heat pump (3) through which technical fluids for room heating and / or for domestic water production circulate, and an associated method for managing and controlling the switching cycle (ON / OFF) of said heat pump (3) during domestic hot water (DHW) intake. The method comprises at least the following steps: during said domestic hot water (DHW) water intake, ensuring flow to the heat pump (3) at a flow rate mchHP at least adapted to avoid closing thereof, and ensuring a complete achievement of this DHW water intake.

The invention discloses a method for detecting L-cysteine in serum based on N, S co-doped carbon nanodots, and belongs to the field of analysis and detection. The method comprises the following steps: (1) preparing carbon dots by taking beta-mercaptoethylamine and p-phenylenediamine as precursors; (2) after Fe < 3 + > is added into the carbon dot solution, a carbon dot fluorescence quenching phenomenon occurs, and a carbon dot-Fe < 3 + > mixed system is obtained; then adding L-cysteine into the carbon dot-Fe < 3 + > mixed system to gradually recover the fluorescence of the system; and (3) constructing a linear relationship between the concentration of the L-cysteine and the fluorescence recovery degree to obtain the quantitative detection model for the L-cysteine in the serum sample. According to the method, the carbon dots are prepared by taking beta-mercaptoethylamine and p-phenylenediamine as precursors for the first time, quantitative detection of L-cysteine is realized, and the method is low in cost, high in sensitivity, suitable for field rapid detection and relatively high in practical value and popularization prospect.

The invention discloses a wave-absorbing material chemical coprecipitation reaction kettle, and relates to the technical field of chemical engineering equipment and functional material preparation, the reaction kettle comprises the following components: a three-stage gradient reaction cavity structure, an anti-pollution structure and a multi-physics field coupling control platform; through the design of a three-stage gradient reaction cavity structure, optimal distribution of a fluid field is realized, stable and efficient flowing of a reaction solution among different cavities is ensured, a multi-axis intelligent mixing system in a premixing area can quickly mix a precursor solution to achieve a molecular-level mixing state, an ideal initial condition is provided for subsequent reaction, and the reaction efficiency is improved. The nucleation area realizes uniform nucleation of nanocrystal nuclei through accurate temperature control and chemical environment control, the grain size distribution range is effectively reduced, the growth area controls preferred growth of crystal faces through adjustment of magnetic field intensity and solution supersaturation degree, particles with good monodispersity are obtained, and under the combined action of the designs, the particle size distribution range is effectively reduced. The reaction efficiency and the consistency of the product quality are obviously improved.

The invention provides a long-acting antibacterial / antioxidant biomass-based essential oil microcapsule and a preparation method thereof. The preparation method comprises the following steps: firstly, uniformly stirring and mixing a chitosan stock solution and a casein stock solution, adjusting the pH value of a mixed system, and promoting the formation of nanoparticles through reverse charge neutralization of casein and chitosan; centrifuging the mixed solution, collecting a supernatant, slowly dripping essential oil into the supernatant, stirring and emulsifying, and ultrasonically preparing an emulsion; and finally, adding a natural cross-linking agent for cross-linking, and filtering and drying after reaction to obtain the long-acting antibacterial / antioxidant biomass-based essential oil capsule. The method does not need an additional emulsifier, is simple in process and low in cost, and is suitable for the fields of drug delivery, cosmetics and food preservation. The prepared microcapsule has the advantages of better stability, high coating rate, good slow release performance, long-acting antibacterial / antioxidant property and the like.

The invention provides a modified separation membrane as well as a preparation method and application thereof, the preparation method of the modified separation membrane comprises the following steps: obtaining a mixed system of a mineral aggregate and a polar solvent, performing ultrasonic liquid phase stripping on the mixed system to obtain a to-be-treated liquid, and the mass volume ratio of the mineral aggregate to the polar solvent is (50-100) mg: (30-60) ml; performing centrifugal treatment on the to-be-treated liquid, and collecting supernatant liquid to obtain nano suspension liquid; a cross-linking agent is mixed with the nano suspension to obtain modified liquid, the modified liquid contains the modified nanosheets, and the mass of the cross-linking agent is 2%-30% of the mass of the mineral aggregate; the modified liquid is filtered through a filter membrane, the modified nanosheets are evenly loaded on the surface of the filter membrane, and the modified separation membrane is prepared. The modified separation membrane prepared by the invention is a modified separation membrane material with high separation coefficient, high rejection rate and high flux, and has excellent performance.

The application discloses a kind of AuNPs@ polyimide SERS heating chip preparation and application method, through interface self-assembly technology preparation large-scale AuNPs array film, and transfer to the polyimide film with heating system, form AuNPs@ polyimide heating film as APHC for SERS chip, in situ heat enrichment is carried out to TNT molecule by APHC, and sample can be effectively collected from mixed system by simple operation.This flexible, heatable SERS chip has great significance for efficient, sensitive and in-situ detection of target molecules in complex environmental systems.

The invention provides a mixed speech recognition method and system based on an AI large model architecture. The method comprises the following steps: constructing a mixed system comprising a traditional ASR system and an ASR system of an AI large model; based on a large amount of unlabeled audio data, pre-training the large model through self-supervised learning, mining audio signal features, and performing supervised fine tuning on the AI large model; using cross entropy loss to train text output in the base layer; an acoustic modeling branch is introduced into the middle layer or the auxiliary head, and a CTC loss function is adopted to train acoustic output; combining a CTC loss function with cross entropy loss training to realize acoustic and text information fusion; in a model training or reasoning stage, an audio partitioning mode and a KV cache technology are adopted to realize efficient streaming identification; and the recognition result can be obtained while speaking, and after the calling is finished, the large model quickly completes the subsequent autoregression text reasoning based on all cache information, and outputs a high-precision recognition text. According to the invention, compatibility of high precision and streaming experience is realized.

The invention discloses a method for preparing a b-axis oriented MFI type molecular sieve separation membrane through in-situ crystallization. The method comprises the following steps: firstly, preparing a graphite transition layer on the surface of a porous carrier, then directly putting the modified porous carrier into a high-pressure synthesis kettle filled with synthesis liquid, crystallizing, washing with deionized water, and drying to obtain a b-axis oriented MFI type molecular sieve separation membrane product. According to the present invention, with the in-situ crystallization method, the seed crystal synthesis in the secondary growth method and the construction process of the carrier surface orientation seed crystal layer are avoided, the preparation process of the b-axis orientation MFI type molecular sieve membrane is simplified, and the obtained product can be used for the separation of the ethanol / water and other mixed systems, and has important application value in the actual chemical industry production field.

To provide a mixing system 1 for a power storage device that efficiently homogenizes multiple types of powder.SOLUTION: A mixing system 1 for a power storage device includes a measuring portion 10 that stores multiple types of powder P measured at a predetermined ratio, a supply portion 20 that is arranged below the measuring portion 10 and supplies the powder P to a kneading portion 30, and the kneading portion 30 that is connected to the supply portion 20 and kneads the powder P supplied from the supply portion 20. The measuring portion 10 includes a lower opening 10c that opens downward, a shutter 11 that is arranged at an opening edge 10d of the lower opening 10c, and a control portion 13 that opens and closes the lower opening 10c by sliding a shutter 11 in an opening / closing direction A. The opening speed, which is the speed at which the shutter 11 moves in the opening direction B, is set on the basis of the volume ratio of the powder P in each of multiple compartments obtained by dividing the powder P stored in the measuring portion 10 into multiple compartments in the opening / closing direction A.SELECTED DRAWING: Figure 1

The invention relates to the technical field of conductive adhesives, in particular to a graphene doping-based flexible conductive adhesive and a preparation method thereof. The invention overcomes the defects of poor electrical conductivity and poor thermal conductivity of the existing adhesive. The preparation method comprises the following steps: mixing epoxy resin, carboxyl-terminated liquid nitrile rubber and 1, 4-butanediol diglycidyl ether, adding modified epoxy resin, and continuously mixing to obtain a mixed system; adding a nano-alloy into the mixed system, mixing, and carrying out first-stage high-shear dispersion to obtain a primary mixed material; adding a few-layer graphene and silver powder mixture into the primary mixture, stirring, and performing two-stage high-shear dispersion, low-frequency vibration treatment and vacuum defoaming treatment to obtain the flexible conductive adhesive. Wherein the modified epoxy resin is prepared from modified graphene oxide and epoxy resin; the modified graphene oxide is prepared from graphene oxide and a silane coupling agent. The adhesive prepared in the invention has strong electrical conductivity and strong thermal conductivity, and is suitable for bonding optical communication equipment components.

The invention discloses a radioactive liquid activity detection method, device and equipment, and relates to the field of nuclear energy. The method comprises the following steps: acquiring a to-be-detected water sample, and respectively inputting the to-be-detected water sample into a detector to obtain a detector original pulse signal and a detector fast signal corresponding to the to-be-detected water sample; performing FPGA time window coincidence processing based on the detector original pulse signal and the detector fast signal to obtain a new energy spectrum corresponding to the to-be-detected water sample; obtaining a preprocessed theoretical energy spectrum template, and inputting the theoretical energy spectrum template and the new energy spectrum into the peak decomposition network model to obtain a characteristic peak area list of the target nuclide; and obtaining an efficiency curve of the detector, and obtaining an activity detection result of the target nuclear element in the to-be-detected water sample based on the characteristic peak area list of the target nuclide and the efficiency curve. According to the method, the final result is determined by combining the theoretical energy spectrum template, the original pulse signal of the detector and the fast signal of the detector, so that the spectrum unfolding error of the overlapping peak can be reduced, and the recognition accuracy of the mixed system is effectively improved.

The invention provides an anti-aging composition containing sea cucumber peptide and a preparation method of the anti-aging composition, and belongs to the technical field of cosmetics. Mixing the sea cucumber peptide compound, the fermented smallanthus sonchifolius extract, the grape seed extract and the clam peptide at 23-25 DEG C for 10-15 minutes to obtain a mixture; the preparation method comprises the following steps: dispersing xanthan gum and sodium carboxymethyl cellulose in purified water at 85-90 DEG C, and stirring and mixing for 20-30 minutes to obtain a mixed system A; mixing the mixed system A, sodium hyaluronate, butanediol, hydroxyethyl urea, stearyl alcohol polyether-21 and glycosylglycerol at 50-55 DEG C for 10-15 minutes to obtain a mixed system B; and mixing the mixed system B and the mixture at 40-45 DEG C for 20-30 minutes to obtain the anti-aging composition. The sea cucumber peptide and the clam peptide jointly stimulate collagen synthesis through the synergistic effect, and good anti-aging and moisturizing effects are achieved through the synergistic effect of multiple components.

The present invention belongs to the technical field of battery material preparation, and specifically discloses a hard carbon material, a preparation method thereof and an application. The preparation method of the hard carbon material provided by the present invention impregnates the pore-filling carbon source mixed system into the large and small pores in the activated carbon under high temperature and high pressure. The pore-filling carbon source deposits into small molecule fragments in the pores of the activated carbon through a chemical reaction, and then rapidly raises the temperature to a specific temperature and keeps it warm, so that the pore-filling carbon source decomposes to form a carbon matrix to block the pores. Moreover, the disordered carbon atoms in the activated carbon can also form a large number of graphite domains. During the formation of the graphite domains, due to the difference in the nucleation energy barriers of different carbon atoms, there is a strong electrostatic repulsion between the layers, thereby broadening the interlayer spacing. The preparation method provided by the present invention greatly improves the fast charging performance of the hard carbon material when applied to the electrode sheet by reducing the closed pore volume and broadening the interlayer spacing.

The invention relates to the technical field of environmental analytical chemistry, and discloses a thymol phenolphthalein-TBPE double indicator-based cyanide titration detection method and system, and the method comprises the following steps: adjusting a water sample to be detected to an alkaline pH interval to construct a detection matrix; the method comprises the following steps: introducing a double-indicator system composed of thymol phenolphthalein and tetrabromo phenolphthalein ethyl ester according to a specific ratio into a detection matrix, and constructing a spectrum background for masking yellow interference of sulfide by utilizing the blue color development characteristic of thymol phenolphthalein; dropwise adding a silver nitrate standard solution into the mixed system to execute a complex reaction; according to the method, a spectrum background shielding field is constructed, chromaticity response of a system in a specific wave band is monitored in real time, and when a chromaticity mutation signal from green to bluish violet is captured, a titration end point is judged, and the cyanide concentration is calculated. High-precision anti-interference detection on cyanide in a complex high-turbidity matrix is realized, and the sensitivity and accuracy of end point recognition are improved.

The invention provides a water pollution real-time detection and early warning method based on self-adaptive spectral feature fusion, relates to the technical field of environmental monitoring, and aims at spectral data of different incident angles through a self-adaptive segmented spectral enhancement algorithm, a feature enhancement strategy is dynamically adjusted, complementarity of multi-angle spectral information is effectively mined, and the real-time detection and early warning of water pollution are realized. Therefore, the precision and the anti-interference capability of heavy metal ion concentration detection are remarkably improved. And the heavy metal recognition model carries out accurate correction on the spectrum superposition effect and matrix interference in the heavy metal mixed system through the spectrum interference suppression factor, so that the robustness and reliability of concentration detection are further improved. Rapid detection and real-time early warning of pollutant concentration are realized, and the problem of low detection precision caused by mutual interference of spectral characteristics of mixed pollutants of heavy metal ions (especially chromium ions and nickel ions) in industrial wastewater can be solved to a certain extent.

The present disclosure relates to a mixing system that includes an injector body comprising a first flow path configured to transmit a motive fluid, a second flow path configured to transmit a granular material, and a diffuser configured to transmit and mix the motive fluid of the first flow path with the granular material of the second flow path. A flexible vessel includes a closed end, an open end, and an interior volume configured to hold a granular material. The open end is configured to couple to the injector body at or adjacent the second flow path. A pressurized fluid inlet is configured to fluidly couple to the second flow path and configured to transmit a pressurized fluid to at least one of the interior volume of the flexible vessel and the second flow path.

This invention relates to a polyurethane foam material, its preparation method, and its application. The method includes the following steps: (1) preparing a polyol solution with toluene, and then mixing the polyol solution with an aqueous solution of particulate material to obtain an emulsion; (2) adding isocyanate and a catalyst to the emulsion and stirring evenly to obtain a mixed system; (3) foaming the mixed system to obtain a polyurethane foam material. This invention overcomes the shortcomings of existing polyurethane foaming processes that require the use of surfactants (foam stabilizers) as emulsifiers, to stabilize foam and adjust cell size, and the difficulties in material stirring and dispersion caused by high solution viscosity. This invention provides a method for preparing a polyurethane foam material that is surfactant-free, has low solution viscosity, and is safe and efficient. The polyurethane foam prepared by this invention has excellent performance, small foam pore size, and a wide range of applications.

The invention provides a lithiated clay mineral PVDF-based composite solid-state electrolyte membrane, a preparation method and application thereof, and a quasi-solid-state battery, and belongs to the technical field of solid-state lithium batteries. The preparation method comprises the following steps: mixing lithium halide, clay minerals and water for the first time, and carrying out ion exchange reaction to obtain lithiated clay minerals; mixing lithiated clay minerals, PVDF, organic lithium salt and an organic solvent for the second time to obtain composite slurry; and applying the composite slurry to the surface of a substrate, and carrying out thermocuring to obtain the lithiated clay mineral PVDF-based composite solid electrolyte membrane. The lithiated clay mineral is introduced into a mixed system of PVDF and organic lithium salt, so that the crystallinity of PVDF can be effectively reduced, an amorphous region is increased, conversion of PVDF crystals to beta-type PVDF is promoted, and the dissociation process of the lithium salt in the electrolyte is promoted; meanwhile, the lithiated clay mineral is introduced, so that the ionic conductivity can be obviously improved, an electrochemical window is widened, the mechanical property is improved, and the growth of lithium dendrites is inhibited.

The invention provides an extracellular vesicle marker separation-free detection method based on nanoparticle enhancement, and belongs to the technical field of detection methods, the method comprises the following steps: preparing gold nanoparticles with the surface modified with a first antibody, the first antibody being used for specific recognition of an extracellular vesicle surface marker; determining the particle size distribution of the gold nanoparticles by adopting a dynamic light scattering method, and determining the surface potentials and surface isoelectric points of the gold nanoparticles by adopting a potentiostat; adopting a transmission electron microscope to characterize the morphology of the screened gold nanoparticles; co-modifying polyethylene glycol molecules on the surfaces of the gold nanoparticles to form an anti-interference layer; mixing the gold nanoparticles with uniform morphology with a sample to be detected; adding a second antibody into the mixed system; distinguishing signals of the binding-state second antibody and the free-state second antibody through fluorescence lifetime analysis; preparing an extracellular vesicle standard substance, and obtaining a standard curve and a system response curve; the optimal detection condition is determined by solving the detection system optimization equation set, and separation-free detection is carried out.

The invention relates to the technical field of in-vitro diagnostic kits, in particular to hollow porous monodisperse carboxyl polystyrene microspheres, a preparation method and application of the hollow porous monodisperse carboxyl polystyrene microspheres in a kit for quantitatively detecting MxA. The preparation method of the microspheres comprises the steps that firstly, hydrophilic polystyrene microspheres are prepared to serve as stable particles; then fully mixing the Pickering particles with a first oil phase to form Pickering stable particles; then a surfactant and a second oil phase are added, the mixed system is stirred at a high speed, and the second oil phase permeates into the surfaces or pores of Pickering stable particles through interface interaction and molecular diffusion; under the action of an initiator, the polymeric monomers are subjected to a polymerization reaction, the low-boiling-point organic solvent is evaporated out along with temperature rise, and a polymer with a core part of a hollow structure and a shell layer of a porous structure is formed; and adding a carboxyl-containing functional monomer to form a hydrophilic polymer chain on the surface of the polymer, thereby finally obtaining the hollow porous monodisperse carboxyl polystyrene microspheres.

Mixing systems that include a mixer housing and a plurality of roller assemblies for mixing and processing materials is disclosed. Each roller assembly includes a roller that extends between sidewalls in the mixer housing and rotates to mix an additive into the material and to carry agglomerated solids toward a discharge of the mixing system. Each of the rollers may have a protrusion geometry formed in the outer contact surface, where each protrusion geometry is complementary to an adjacent roller.