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244results about How to "Stable quantity" patented technology

Multi-port regional gridding VRS (Virtual Reference Station) differential positioning information broadcasting device and work method thereof

The invention relates to a multi-port regional gridding VRS (Virtual Reference Station) differential positioning information broadcasting device and a work method thereof, belonging to the field of measuring methods. The multi-port regional gridding VRS differential positioning information broadcasting device comprises a network RTK (Real Time Kinematic) system reference station, a data processing center and a data FM (Frequency Modulation) broadcasting unit, wherein the network RTK system reference station is connected with a data processing center server through optical fiber, and the data processing center server is connected with the data FM broadcasting unit through a serial port; and the data FM broadcasting unit is used for carrying out FM broadcasting on differential correction data packaged by a UDP (User Datagram Protocol). The work method comprises the steps of: receiving network RTK system reference station information by the data processing center, and dividing grids for the covered region of the network RTK system reference station; then solving all differential correction information of all grid virtual reference stations by utilizing a VRS algorithm, and then appointing an unique UDP port number for each grid virtual reference station and packaging the differential correction information by using the UDP protocol; finally, carrying out FM broadcasting on the UDP data packets, three-dimensional coordinates and UDP port numbers of all the grid virtual reference stations through the data FM broadcasting unit.
Owner:SHANDONG UNIV

Image forming apparatus

An image forming apparatus includes an image bearing member on which an electrostatic image is to be formed; a developing device for developing the electrostatic image with a developer; a developer container detachably mountable to a mounting portion provided in a main assembly of the apparatus, for accommodating a supply of developer; a discharging device for discharging the supply of developer from the developer container mounted to the mounting portion to an outside of the developer container; a first developer accommodating portion for receiving the developer from the discharging device and for accommodating an amount of the developer which exceeds a first predetermined amount, wherein the first amount is larger than a capacity of the developer container; a first detecting device for detecting an amount of the developer in the first accommodating portion; a first supplying device for supplying the developer in the first accommodating portion to an outside of the first accommodating portion; a second developer accommodating portion for receiving the developer from the first supplying device and for accommodating an amount of the developer which exceeds a second predetermined amount; a second detecting device for detecting the amount in the second accommodating portion; a second supplying device for supplying the developer from the second accommodating portion toward the developing device; a controller for controlling, when the developer is accommodated in the developing container, the discharging device such that amount of the developer in the first accommodating portion is not less than the first amount, in accordance with a detection result of the first detecting device and such that such that amount of the developer in the second accommodating portion is not less than the second amount, in accordance with a detection result of the second detecting device, and for permitting, when the developing container is substantially empty, and the amount in the first accommodating portion is less than the first value, an image forming operation to continue until the amount in the second developer portion becomes less than the second amount.
Owner:CANON KK

Primary isolation culture method of adipose-derived stem cells

The invention discloses a primary isolation culture method of adipose-derived stem cells. The primary isolation culture method comprises the following steps: (1) washing adipose tissues; (2) adding a mixed liquid of type-I collagenase and trypsase into the adipose tissues, wherein the volume of the mixed liquid is equal to the volumes of the adipose tissues; (3) adding FBS, covering a bottle cap, sealing, uniformly shaking, carrying out centrifugation, and discarding supernate after the centrifugation; (4) carrying out resuspension precipitation by virtue of a DMEM containing the FBS, uniformly blow-hitting to form cell suspension, and inoculating the cell suspension to a culture medium, wherein the volume ratio of precipitates to the DMEM containing the FBS is 1 to 1; (5) culturing the inoculated culture medium; and (6) transferring the cultured culturing medium into a cell incubator, and continuing to culture the culturing medium. Enzymolysis conditions explored in the primary isolation culture method are stable and efficient, the complete enzymolysis of the adipose tissues is achieved, and cells of each component are furthest released; on the premise that the activity of ADSCs is not influenced, the quantity of the ADSCs acquired in unit adipose volume is relatively stable.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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