52 results about "Nanoparticle Complex" patented technology

The invention discloses a method for preparing a micro-particle-size negative oxygen ion purifying agent from a nano material, and relates to the technical field of air purifying agents, the method comprises the following preparation steps: step 1, pouring 5-6.5 g of modified tourmaline powder and 10 g of an amino acid-nano particle compound into 200 mL of deionized water, then adding 0.5-0.54 g of polyvinylpyrrolidone, carrying out ultrasonic treatment for 30-40 min, then adding 0.5-0.54 g of polyvinylpyrrolidone into the deionized water, carrying out ultrasonic treatment for 30-40 min, and carrying out ultrasonic treatment for 30-40 min; carrying out ball milling until the particle size is 1-5 microns; 2, adding 2-2.6 g of a chitosan solution with the concentration of 1% after ball milling, adjusting the pH value of the system to 6, and stirring at 50 DEG C for 1 h; according to the method for preparing the micro-particle-size negative oxygen ion purifying agent from the nano material, tourmaline powder is modified, so that the surface hydroxylation degree of the tourmaline powder is improved, lattice defects are increased, the negative oxygen ion release efficiency is remarkably improved, meanwhile, the tourmaline powder is compounded with white carbon black, interface charge conduction can be enhanced, and continuous release of negative oxygen ions is further promoted.

The invention provides magnesium manganese carbonate nanoparticles wrapped by cell membrane vesicles as well as a preparation method and application of the magnesium manganese carbonate nanoparticles. The nanoparticles comprise tumor cell-derived membrane vesicles highly expressing PD1; the MgMn nano-particle compound is wrapped in the vesicles; the magnesium manganese carbonate nano-particles (CVs (at) MgMn) wrapped by the cell membrane vesicles provided by the invention integrate numerous advantages of Mg < 2 + > and Mn < 2 + > ions, not only have immune regulation and control and tumor killing effects, but also can realize radiotherapy sensitization, have immune regulation and control and microenvironment remodeling effects on any type of solid tumors, and can be used for preparing a medicine for treating tumor tumors. And the nano-particles are universal multifunctional composite nano-particles.

The invention relates to the technical field of preparation of IL-12 slow-release hydrogel, in particular to a preparation method and system of IL-12 slow-release hydrogel based on nanoparticles, and the preparation method comprises the following steps: obtaining high-quality nanoparticles, carrying out irradiation sterilization on the high-quality nanoparticles to obtain sterilized nanoparticles, obtaining a drug-loaded nanoparticle compound, and preparing a hydrogel base material solution; the method comprises the following steps: obtaining an optimal mixing ratio, confirming divalent cation parameters, preparing a crosslinking solution, preliminarily mixing the crosslinking solution and a hydrogel precursor solution to obtain an initial mixed solution, detecting the initial mixed solution to obtain crosslinking performance parameters, and if the crosslinking performance parameters meet preset standard crosslinking performance parameter interval conditions, determining that the hydrogel is in the crosslinking state. If not, taking the initial mixed solution corresponding to the optimal crosslinking performance parameter as the optimal IL-12 slow-release hydrogel, and completing the preparation of the IL-12 slow-release hydrogel based on the nanoparticles based on the optimal IL-12 slow-release hydrogel. The in-vivo and in-vitro slow release effect of the IL-12 can be improved.

The present invention provides methods, compositions, systems, and kits comprising nano-satellite complexes and / or serum albumin carrier complexes, which are used for modulating antigen-specific immune response (e.g., enhancing anti-tumor immunity). In certain embodiments, the nano-satellite complexes comprise: a) a core nanoparticle complex comprising a biocompatible coating surrounding a nanoparticle core; b) at least one satellite particle attached to, or absorbed to, the biocompatible coating; and c) an antigenic component conjugated to, or absorbed to, the at least one satellite particle component. In certain embodiments, the complexes further comprise: d) a type I interferon agonist agent. In some embodiments, the serum albumin complexes comprise: a) at least part of a serum albumin protein, b) an antigenic component conjugated to the carrier protein, and c) a type I interferon agonist agent.

The application provides a self-driven BCG-nanoparticle complex and a preparation method thereof, and belongs to the fields of biological medicine and nanomaterials. The complex is a composite of BCG and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the BCG in an asymmetric distribution through modified polyethyleneimine, and specifically, the surface of the BCG is connected to several modified polyethyleneimine derivatives through chemical bonds and electrostatic adsorption, and the polyethyleneimine derivatives wrap the cerium oxide nanoparticles through electrostatic adsorption. The complex can be self-driven by using the gas release and ion concentration change caused by the catalytic decomposition of urea in the environment, significantly enhances the adhesion and retention of BCG on the bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of traditional BCG therapy, simultaneously improves the tumor immune microenvironment, and can be used for preparing high-efficiency and low-toxicity bladder cancer treatment drugs.

Orally administrable nanoparticle complexes of biological molecules and biopolymers are described herein, and include formulations for the oral administration of peptides, proteins, nucleic acids, and antibodies.

The application discloses a urine small extracellular vesicle PLA2R1 membrane protein detection method for distinguishing membranous nephropathy, and belongs to the technical field of biomedical detection. The detection method comprises the following steps: preparing an anti-CD63 antibody surface fixed plasmonic metasurface sensing chip to directly capture small extracellular vesicles in urine; preparing an anti-PLA2R1 antibody modified biofunctionalized gold nanoparticle complex to specifically combine with the PLA2R1 membrane protein on the surface of the small extracellular vesicles; detecting the reflection spectrum of the chip after antibody fixation and gold nanoparticle combination through a spectrometer with an integrated optical fiber probe; calculating the resonance wavelength shift amount; measuring the expression level of the PLA2R1 membrane protein; and realizing accurate distinction of healthy people, membranous nephropathy gray area and confirmed patients. The non-invasive detection method is simple in operation and rapid in detection, the sensitivity and the specificity are improved by means of the gold nanoparticle signal amplification effect, and is suitable for large-scale clinical screening and early diagnosis.

The present invention provides nanoparticles for targeted elimination of intracellular bacteria and a preparation method thereof. The nanoparticles are composed of a biodegradable nanoparticle matrix, a potassium ion-responsive linear polymer grafted onto the surface of the nanoparticle matrix, and a drug loaded into the pore structure of the nanoparticle matrix. The nanoparticle matrix has a pore structure, the drug is a drug for treating bacterial infection, and the potassium ion-responsive linear polymer can achieve conformational transition and charge reversal after recognizing and responding to potassium ions. The nanoparticles are taken into the cells through the endocytosis of macrophages. In the high potassium ion concentration environment within the cells, the potassium ion-responsive linear polymer on the surface of the nanoparticles complexes with potassium ions, causing the nanoparticles, which are negatively charged in the extracellular fluid, to undergo charge reversal within the cells, thereby inducing the nanoparticles to target and bind to the intracellular bacteria, while simultaneously undergoing conformational transition and releasing the loaded drug.

The present application belongs to the field of medical material surface functionalization, and particularly relates to a kind of nano drug-loaded hydrogel catheter coating and its preparation method and application.The present application utilizes PLGA to load antibiotic amikacin sulfate AMK or antibacterial peptide FK13-a1, synthesizes nanoparticle NP-AM and nanoparticle NP-FK, prepares NP-AM / FK@OMV nanoparticle complex with EcN OMV as carrier, then adds poloxamer-hyaluronic acid composite hydrogel to obtain NP-AM / FK@OMV-P / H nano drug-loaded hydrogel, and forms coating after coating and drying.The nano drug-loaded hydrogel coating provided by the present application has the characteristics of enzyme response, antibacterial, prevention of biofilm formation and low toxicity, has great application potential in inhibiting medical catheter surface biofilm, and has important significance for preventing and treating CAUTI, prolonging the use time of indwelling catheter, and reducing the discomfort of patients caused by frequent replacement of catheter.

A nanoparticle complex comprises a bifunctional conjugate capable of non-covalently binding to lipid nanoparticles in the nanoparticle complex.

The present invention discloses a photothermal active starch-based food label and its preparation method, belonging to the technical field of packaging material processing. The method of the present invention is a preparation method of nanoparticles based on the in-situ self-polymerization of catecholamine-like substances and the coordination reaction of metal polyphenols. Under the action of the graphene π-π conjugation platform, the catecholamine-like substances are promoted to undergo in-situ self-polymerization with metal salts at the graphene interface to form a double-spherical metal polyphenol nanoparticle complex. At the same time, with the help of the amino-hydroxy interaction, the cyclodextrin cavity is adsorbed on the modified graphene interface. This cavity can adsorb fat-soluble volatile plant essential oils. The nanoparticles are dispersed in the starch-based film, and the starch-based film is complete and continuous. Under near-infrared light irradiation, it can be heated from 20°C to 200°C within 30 seconds, and the mechanical tensile strength reaches 28 MPa. It has excellent photothermal conversion performance and antibacterial performance, and can be used in the fields of food, medicine, medical devices, and agricultural product packaging.

The invention provides a self-driven bacillus calmette-guerin vaccine-nano enzyme compound and a preparation method thereof, and belongs to the field of biological medicines and nano materials. The compound is a compound of bacillus calmette-guerin and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the bacillus calmette-guerin through modified polyethyleneimine and are asymmetrically distributed, specifically, the surface of the bacillus calmette-guerin is connected with a plurality of modified polyethyleneimine derivatives through chemical bonds and an electrostatic adsorption effect, and the modified polyethyleneimine derivatives are coupled to the surface of the bacillus calmette-guerin. The cerium oxide nano particles are wrapped by the polyethyleneimine derivative through an electrostatic adsorption effect. The compound provided by the invention can realize self-driving by utilizing gas release and ion concentration change caused by urea catalytic decomposition in an environment, remarkably enhances the adhesion and retention of BCG on a bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of a traditional BCG therapy, improves a tumor immune microenvironment, and improves the tumor immune activity. The compound can be used for preparing high-efficiency and low-toxicity bladder cancer treatment medicines.

The purpose of the present invention is to provide composite particles having nanoparticles that are excellent in terms of weather resistance. Composite particles according to an embodiment of the present invention each include at least one nanoparticle complex and an aggregate of a plurality of functional particles. The nanoparticle complex is present inside an aggregate structure that is formed of the aggregate of the functional particles, and the nanoparticle complex is held inside the aggregate structure by intermolecular force between the nanoparticle complex and the functional particles.

The present invention relates to the technical field of nanodevice preparation, and specifically to a method for batch preparation of enzyme-driven Janus nanomotors, comprising the following steps: preparing an asymmetric gold nanoparticle complex by electrostatic adsorption; modifying the surface of the gold nanoparticles with CHO-PEG-SH or acetylcysteine; separating and removing the magnetic microparticles electrostatically adsorbed to the gold nanoparticles to obtain CHO-PEG-SH-nanogold particles or acetylcysteine-nanogold particles; further modifying the nanoparticles with CHO-PEG-SH or acetylcysteine ​​to obtain gold nanoparticles modified with CHO-PEG-SH and acetylcysteine ​​on both sides; and mixing and shaking the enzyme solution to obtain the enzyme-driven Janus nanomotors. The method is simple, efficient, low-cost, and reusable. It does not require the use of large amounts of organic solvents, can be mass-produced, and is suitable for industrial production.

The present invention relates to a phosphorylated tau-gold nanoparticle complex, a method for manufacturing the same, and a drug screening method using same. The phosphorylated tau-gold nanoparticle complex manufactured according to the method for manufacturing a phosphorylated tau-gold nanoparticle complex of the present invention enables evaluation of how effectively a specific drug degrades phosphorylated tau aggregates and thus can be advantageously used for high-throughput screening of drugs for preventing or treating primary tauopathies.

The application relates to the technical field of biological medical nanomaterial manufacturing, and discloses a polypeptide-cerium dioxide active nanodot compound, which is prepared through electrostatic action of polypeptide (KTTKS) with the function of activating collagen and CeO2 nanodots with the functions of absorbing ultraviolet rays and removing free radicals. The nanodot compound has the advantages of easy cell uptake, ultraviolet resistance, high free radical removal activity, can significantly protect skin cells from ultraviolet damage, and can promote the synthesis of collagen in skin cells. The application has significant practical value in the fields of anti-skin photoaging skin care products and the like.

The invention discloses a preparation method of amorphous zirconium oxide with a chemical power treatment effect, which is a novel nano particle material taking pharmaceutical chemistry as a synthesis basis and is used for tumor treatment by utilizing the special excellent properties of the amorphous zirconium oxide. The particle synthesis mainly utilizes chemical reaction to prepare nano amorphous zirconium dioxide. The specific preparation method of the nanoparticles comprises the following steps: 1) stirring zirconium oxychloride octahydrate and ammonia water at room temperature, 2) reacting through a high-pressure reaction kettle at a specific temperature, and 3) centrifugally drying and collecting materials.The finally obtained nanoparticle complex has good biocompatibility, biological safety and a good tumor treatment effect.

The invention belongs to the crossing field of functional food and nanotechnology, and particularly relates to a curcumin-coffee nanoparticle compound and a preparation method and application thereof, the curcumin-coffee nanoparticle compound comprises coffee nanoparticles and curcumin, the curcumin is loaded on the surfaces of the coffee nanoparticles through non-covalent acting force; wherein the coffee nano-particles are natural nano-particles separated from a coffee extracting solution through an ultrafiltration method. Natural nanoparticles contained in coffee are used as a carrier, and curcumin is loaded on the surface of the carrier by adopting a pH regulation method, so that the stability of a final compound in a milk system is remarkably improved, aggregation and precipitation phenomena caused by phenolic acid-protein interaction are effectively inhibited, and the beverage keeps uniform texture.

A method of identifying one or more bacteria from a sample, where the one or more bacteria is suspected to be present in the sample. An aqueous suspension including a bacteria-SERS nanoparticle complex is provided, where the bacteria-SERS nanoparticle complex includes (i) a SERS nanoparticle and (ii) the one or more bacteria suspected to be present in the sample. Furthermore, the aqueous suspension is deposited on a substrate having structures coated with a SERS-active material to dispose the bacteria-SERS nanoparticle complex on the substrate. Additionally, the bacteria-SERS nanoparticle complex disposed on the substrate is irradiated to generate one or more SERS signals. Furthermore, a SERS-spectroscopic module is operable to render one or more SERS spectral data corresponding to the one or more SERS signals. Additionally, a process module is operable to identify the presence or absence of the one or more bacteria from the one or more SERS spectral data.

The invention discloses a difunctional nanoparticle and a construction method thereof in the technical field of biosensors. The nanoparticle comprises a ferroferric oxide magnetic core, a mesoporous silicon dioxide shell layer coated on the surface of the magnetic core, palladium nanoparticles modified on the surface of the silicon dioxide shell layer, and luminol molecules coupled to the silicon dioxide shell layer through covalent bonds. The material has superparamagnetism, a high specific surface area, a mesoporous structure and efficient peroxide activity, can accelerate hydrogen peroxide to generate hydroxyl radicals (OH), and can promote luminol to generate strong electrochemiluminescence signals. The superparamagnetism can be used as a trapping agent for trapping exosomes in body fluid. The PD-L1-modified gold electrode is combined with the exosome-nanoparticle compound, so that the PD-L1 exosome can be sensitively and quickly detected, and the PD-L1-modified gold electrode has important significance in early discovery of non-small cell lung cancer and prediction of the treatment effect of an immune checkpoint inhibitor.

This invention relates to fruit juice flocculants, a chitosan-coffee grounds nanoparticle composite, its preparation and application, comprising the following steps: 1) Obtaining coffee grounds powder: drying and sieving coffee grounds to obtain the desired coffee grounds powder; 2) Dissolving the coffee grounds powder in water, heating, filtering, collecting the filtrate, passing it through a D101 macroporous resin column, eluting with water, dialyzing and drying the eluent to obtain coffee grounds nanoparticles; 3) Mixing a chitosan aqueous solution (chitosan:water) at a mass ratio of 1:100-400 with the coffee grounds nanoparticles and adding it to a reaction vessel for hydrothermal reaction; dialyzing and drying the resulting solution to obtain the chitosan-coffee grounds nanoparticle composite. This invention aims to use nanoparticles with good biocompatibility, low dosage, significant flocculation effect, good antioxidant properties, and visibility to modify chitosan, synthesizing a novel flocculant and providing a new approach for fruit juice clarification.

This invention discloses a lipid-gold nanoparticle complex for treating sepsis. The preparation method includes the following steps: Sodium dodecyl sulfate (SDS) is added to a dispersion of AuNPs and dispersed evenly; β-MEA is added, and stirring is continued for 4–10 hours. SDS is then removed by filtration, and the remaining liquid is concentrated by centrifugation. The concentrate is purified by dialysis. After purification, the pH is adjusted to obtain a cationic AuNPs solution; Claudin-5 overexpression plasmid is added, and the reaction is stirred to obtain an AC solution; soybean lecithin, cholesterol, and DOTAP are added to a round-bottom flask, and methanol solution is added. The mixture is heated to dissolve the liposomes, and the methanol is removed by cooling to obtain liposomes; the AC solution is added to the round-bottom flask, and the mixture is shaken to hydrate. The liposomes are then ultrasonically broken up, purified by centrifugation, and filtered to obtain LAC. A "post-interpolation method" is used to add REDVC-PEG. 2000 DSPE is modified onto the surface of the liposome complex to obtain the RLAC complex that targets vascular endothelial cells.

The invention discloses a surface enhanced Raman spectroscopy (SERS) base material for specific detection of enzyme in a water phase environment, which is haloid / polysaccharide / precious metal nanoparticle compound hydrosol, and is prepared by the following steps: carrying out in-situ reduction reaction on a precious metal compound, polysaccharide and a reducing agent at 10-50 DEG C for 0.5-5 hours to prepare polysaccharide / precious metal nanoparticle compound hydrosol, and carrying out centrifugal concentration to obtain the polysaccharide / precious metal nanoparticle compound hydrosol. And adding a haloid solution to prepare surface modified hydrosol. Hydrosol containing a substrate and precious metal nanoparticles is prepared to serve as an SERS base material, enzyme and the substrate in the SERS base material are subjected to specific binding, then the enzyme is close to the precious metal nanoparticles, and the Raman scattering signal enhancement effect is generated. The haloid / polysaccharide / noble metal nanoparticle compound hydrosol has long-term stability, has a specific surface enhanced Raman effect on enzyme, can detect and analyze the structure of original ecological enzyme in a solution, and has relatively high SERS activity and reproducibility.

The present invention provides methods, compositions, systems and kits comprising nanosatellite complexes, the nanosatellite complexes comprising: a core-nanoparticle complex comprising a biocompatible coating surrounding a nanoparticle core; 3-25 satellite particles attached to or absorbed into the biocompatible coating; a plurality of antigenic peptides conjugated to or absorbed into the satellite particles; and at least one additional characteristic: i) the weight ratio of all the satellite particles to the nanoparticle core is 10-40%; the diameter of each satellite particle is 2-20 nm; iii) the satellite particles are present at a density of 500-20,000 or 15,000-30,000 per square micron; iv) the plurality of antigenic peptides is 100-4000 antigenic peptides; v) 10-300 of the plurality of antigenic peptides are present on each satellite particle; and / or vi) the average distance between each satellite particle is 5-20 nm.

The present invention belongs to the field of biomedical technology, and specifically relates to a membrane protein lipid nanoparticle complex, a preparation method thereof, and an application thereof. The membrane protein lipid nanoparticle complex of the present invention is a nanoparticle liposome complex formed by all or part of the membrane protein antigens expressed on tumor cells and a variety of lipids. This complex can deliver all or part of the membrane protein antigens on tumors to the body through a lipid nanoparticle delivery system, activate the body's immune response, cause a strong humoral and cellular immunity in the body, especially promote the infiltration of CD8-positive T cells and the secretion of IFN-γ in the tumor microenvironment, achieving the effect of preventing or killing tumors, and having no obvious toxic side effects. In addition, when the membrane protein lipid nanoparticle complex of the present invention is used to prepare a tumor vaccine, the carrier delivery efficiency of the membrane protein is high, which can effectively prevent the occurrence of solid tumors or kill cancer cells, and has good application prospects.

The application discloses a kind of nanoparticle coating loaded urinary system implants and its application, belong to the field of biomedicine.The urinary system implant includes support carrier and nanoparticle coating loaded on the support carrier, the siRNA of targeting TGF-β1 gene is contained in the nanoparticle coating, and the siRNA is wrapped in nanoparticle.The siRNA can be transfected to ureter tissue and inhibit the expression of TGF-β1, to inhibit the occurrence of ureteral stricture.The application combines RNA interference technology and nanoparticle delivery system by urinary system implant, and the effect of the urinary system implant loaded with TGF-β1-siRNA nanoparticle complex for inhibiting ureteral stricture is verified by in-vivo and in-vitro experiments, can effectively inhibit the formation of ureteral stricture around urinary system implant, and has wide clinical application prospect.

A quantitative measurement method for a nucleic acid in a nucleic acid-lipid nanoparticle complex, sodium lauryl sulfate serving as a demulsifier. Said quantitative measurement method can accurately and quantitatively determine the effective nucleic acid concentration and the encapsulation rate.

A new nucleic acid delivery system is provided, which comprises a linear peptide rich in histidine-lysine and containing cysteine ​​with a targeting function, and a four-branched histidine-lysine-rich polypeptide. The delivery system comprises a nucleic acid, such as siRNA. These components form a stable nanoparticle complex through non-covalent interactions between the phosphate of the siRNA and the histidine / lysine of the polypeptide, which has reduced toxicity and selectively delivers genetic material to cells. The targeting function enhances the efficiency of nucleic acid delivery and transfection. A carrier molecule is also provided, which can deliver therapeutic molecules to specific cells. The carrier molecule is modified with a targeting ligand, which can bind to a specific receptor present on the targeted cell. The therapeutic molecule is siRNA, miRNA or other oligonucleotides. The targeting moiety is a small molecule, peptide or protein that shows affinity for the receptor present on the targeted cell.

This invention provides a composite polysaccharide-selenium nanoparticle complex, its preparation method, and its applications. The complex comprises polysaccharide-modified selenium nanoparticles, obtained by a redox reaction of a polysaccharide composition with a selenium solution. The polysaccharide composition includes *Boletus edulis* polysaccharide, *Portulaca oleracea* polysaccharide, and *Astragalus membranaceus* polysaccharide, with a mass ratio of 0.5–3:0.5–3:0.5–3. The composite polysaccharide-selenium nanoparticle complex of this invention, through the synergistic modification of selenium nanoparticles by multiple polysaccharides, effectively prevents the aggregation and oxidation of selenium nanoparticles, significantly improves the dispersibility and stability of selenium nanoparticles, enhances their bioavailability and targeting, strengthens their antioxidant activity, reduces their cytotoxicity, and achieves multifunctionality, expanding the application potential of selenium nanoparticles in medicine, food, cosmetics, and other fields.

The invention is suitable for the technical field of food safety, and provides a general detection platform for CRISPR / Cas12a sensing based on Mn < 2 + > regulation engineering crRNA. According to the invention, a Cas12a activity control platform is constructed by virtue of ALP (alkaline phosphatase) and an aptamer modified gold nanoparticle compound in combination with a mechanism of regulating and controlling a crRNA structure by Mn < 2 + >-dependent DNAzyme, and is applied to detection of ALP and staphylococcus aureus. According to the platform, tetravalent manganese is converted into divalent manganese (Mn < 2 + >) to serve as a core signal transduction mode, the sensitivity is high (0.001 U / L ALP can be distinguished, and 10 CFU / mL staphylococcus aureus can be identified), the specificity is high (interference of other non-target bacteria can be effectively eliminated), the detection requirements of the ALP and the staphylococcus aureus can be flexibly met without replacing a core signal mechanism, and the application prospect is wide. The detection result is accurate and reliable.