21 results about "Nanoparticle Complex" patented technology

The present invention provides methods, compositions, systems, and kits comprising nano-satellite complexes and / or serum albumin carrier complexes, which are used for modulating antigen-specific immune response (e.g., enhancing anti-tumor immunity). In certain embodiments, the nano-satellite complexes comprise: a) a core nanoparticle complex comprising a biocompatible coating surrounding a nanoparticle core; b) at least one satellite particle attached to, or absorbed to, the biocompatible coating; and c) an antigenic component conjugated to, or absorbed to, the at least one satellite particle component. In certain embodiments, the complexes further comprise: d) a type I interferon agonist agent. In some embodiments, the serum albumin complexes comprise: a) at least part of a serum albumin protein, b) an antigenic component conjugated to the carrier protein, and c) a type I interferon agonist agent.

The application provides a self-driven BCG-nanoparticle complex and a preparation method thereof, and belongs to the fields of biological medicine and nanomaterials. The complex is a composite of BCG and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the BCG in an asymmetric distribution through modified polyethyleneimine, and specifically, the surface of the BCG is connected to several modified polyethyleneimine derivatives through chemical bonds and electrostatic adsorption, and the polyethyleneimine derivatives wrap the cerium oxide nanoparticles through electrostatic adsorption. The complex can be self-driven by using the gas release and ion concentration change caused by the catalytic decomposition of urea in the environment, significantly enhances the adhesion and retention of BCG on the bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of traditional BCG therapy, simultaneously improves the tumor immune microenvironment, and can be used for preparing high-efficiency and low-toxicity bladder cancer treatment drugs.

Orally administrable nanoparticle complexes of biological molecules and biopolymers are described herein, and include formulations for the oral administration of peptides, proteins, nucleic acids, and antibodies.

The application discloses a urine small extracellular vesicle PLA2R1 membrane protein detection method for distinguishing membranous nephropathy, and belongs to the technical field of biomedical detection. The detection method comprises the following steps: preparing an anti-CD63 antibody surface fixed plasmonic metasurface sensing chip to directly capture small extracellular vesicles in urine; preparing an anti-PLA2R1 antibody modified biofunctionalized gold nanoparticle complex to specifically combine with the PLA2R1 membrane protein on the surface of the small extracellular vesicles; detecting the reflection spectrum of the chip after antibody fixation and gold nanoparticle combination through a spectrometer with an integrated optical fiber probe; calculating the resonance wavelength shift amount; measuring the expression level of the PLA2R1 membrane protein; and realizing accurate distinction of healthy people, membranous nephropathy gray area and confirmed patients. The non-invasive detection method is simple in operation and rapid in detection, the sensitivity and the specificity are improved by means of the gold nanoparticle signal amplification effect, and is suitable for large-scale clinical screening and early diagnosis.

The present application belongs to the field of medical material surface functionalization, and particularly relates to a kind of nano drug-loaded hydrogel catheter coating and its preparation method and application.The present application utilizes PLGA to load antibiotic amikacin sulfate AMK or antibacterial peptide FK13-a1, synthesizes nanoparticle NP-AM and nanoparticle NP-FK, prepares NP-AM / FK@OMV nanoparticle complex with EcN OMV as carrier, then adds poloxamer-hyaluronic acid composite hydrogel to obtain NP-AM / FK@OMV-P / H nano drug-loaded hydrogel, and forms coating after coating and drying.The nano drug-loaded hydrogel coating provided by the present application has the characteristics of enzyme response, antibacterial, prevention of biofilm formation and low toxicity, has great application potential in inhibiting medical catheter surface biofilm, and has important significance for preventing and treating CAUTI, prolonging the use time of indwelling catheter, and reducing the discomfort of patients caused by frequent replacement of catheter.

A nanoparticle complex comprises a bifunctional conjugate capable of non-covalently binding to lipid nanoparticles in the nanoparticle complex.

The invention provides a self-driven bacillus calmette-guerin vaccine-nano enzyme compound and a preparation method thereof, and belongs to the field of biological medicines and nano materials. The compound is a compound of bacillus calmette-guerin and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the bacillus calmette-guerin through modified polyethyleneimine and are asymmetrically distributed, specifically, the surface of the bacillus calmette-guerin is connected with a plurality of modified polyethyleneimine derivatives through chemical bonds and an electrostatic adsorption effect, and the modified polyethyleneimine derivatives are coupled to the surface of the bacillus calmette-guerin. The cerium oxide nano particles are wrapped by the polyethyleneimine derivative through an electrostatic adsorption effect. The compound provided by the invention can realize self-driving by utilizing gas release and ion concentration change caused by urea catalytic decomposition in an environment, remarkably enhances the adhesion and retention of BCG on a bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of a traditional BCG therapy, improves a tumor immune microenvironment, and improves the tumor immune activity. The compound can be used for preparing high-efficiency and low-toxicity bladder cancer treatment medicines.

The present invention relates to a phosphorylated tau-gold nanoparticle complex, a method for manufacturing the same, and a drug screening method using same. The phosphorylated tau-gold nanoparticle complex manufactured according to the method for manufacturing a phosphorylated tau-gold nanoparticle complex of the present invention enables evaluation of how effectively a specific drug degrades phosphorylated tau aggregates and thus can be advantageously used for high-throughput screening of drugs for preventing or treating primary tauopathies.

The invention belongs to the crossing field of functional food and nanotechnology, and particularly relates to a curcumin-coffee nanoparticle compound and a preparation method and application thereof, the curcumin-coffee nanoparticle compound comprises coffee nanoparticles and curcumin, the curcumin is loaded on the surfaces of the coffee nanoparticles through non-covalent acting force; wherein the coffee nano-particles are natural nano-particles separated from a coffee extracting solution through an ultrafiltration method. Natural nanoparticles contained in coffee are used as a carrier, and curcumin is loaded on the surface of the carrier by adopting a pH regulation method, so that the stability of a final compound in a milk system is remarkably improved, aggregation and precipitation phenomena caused by phenolic acid-protein interaction are effectively inhibited, and the beverage keeps uniform texture.

This invention relates to fruit juice flocculants, a chitosan-coffee grounds nanoparticle composite, its preparation and application, comprising the following steps: 1) Obtaining coffee grounds powder: drying and sieving coffee grounds to obtain the desired coffee grounds powder; 2) Dissolving the coffee grounds powder in water, heating, filtering, collecting the filtrate, passing it through a D101 macroporous resin column, eluting with water, dialyzing and drying the eluent to obtain coffee grounds nanoparticles; 3) Mixing a chitosan aqueous solution (chitosan:water) at a mass ratio of 1:100-400 with the coffee grounds nanoparticles and adding it to a reaction vessel for hydrothermal reaction; dialyzing and drying the resulting solution to obtain the chitosan-coffee grounds nanoparticle composite. This invention aims to use nanoparticles with good biocompatibility, low dosage, significant flocculation effect, good antioxidant properties, and visibility to modify chitosan, synthesizing a novel flocculant and providing a new approach for fruit juice clarification.

This invention discloses a lipid-gold nanoparticle complex for treating sepsis. The preparation method includes the following steps: Sodium dodecyl sulfate (SDS) is added to a dispersion of AuNPs and dispersed evenly; β-MEA is added, and stirring is continued for 4–10 hours. SDS is then removed by filtration, and the remaining liquid is concentrated by centrifugation. The concentrate is purified by dialysis. After purification, the pH is adjusted to obtain a cationic AuNPs solution; Claudin-5 overexpression plasmid is added, and the reaction is stirred to obtain an AC solution; soybean lecithin, cholesterol, and DOTAP are added to a round-bottom flask, and methanol solution is added. The mixture is heated to dissolve the liposomes, and the methanol is removed by cooling to obtain liposomes; the AC solution is added to the round-bottom flask, and the mixture is shaken to hydrate. The liposomes are then ultrasonically broken up, purified by centrifugation, and filtered to obtain LAC. A "post-interpolation method" is used to add REDVC-PEG. 2000 DSPE is modified onto the surface of the liposome complex to obtain the RLAC complex that targets vascular endothelial cells.

This invention provides a composite polysaccharide-selenium nanoparticle complex, its preparation method, and its applications. The complex comprises polysaccharide-modified selenium nanoparticles, obtained by a redox reaction of a polysaccharide composition with a selenium solution. The polysaccharide composition includes *Boletus edulis* polysaccharide, *Portulaca oleracea* polysaccharide, and *Astragalus membranaceus* polysaccharide, with a mass ratio of 0.5–3:0.5–3:0.5–3. The composite polysaccharide-selenium nanoparticle complex of this invention, through the synergistic modification of selenium nanoparticles by multiple polysaccharides, effectively prevents the aggregation and oxidation of selenium nanoparticles, significantly improves the dispersibility and stability of selenium nanoparticles, enhances their bioavailability and targeting, strengthens their antioxidant activity, reduces their cytotoxicity, and achieves multifunctionality, expanding the application potential of selenium nanoparticles in medicine, food, cosmetics, and other fields.

The application discloses an electrochemical aptamer sensor for simultaneously detecting Cd 2+ and Hg 2+ as well as a preparation method and application thereof. The electrochemical aptamer sensor comprises a multi-walled carbon nanotube and gold nanoparticle composite modified silk screen printing electrode, methylene blue-labeled MB-Apt is coupled to the electrode surface through gold-sulfur bond covalent action, and Cd 2+ detection is realized through MB electrochemical signal change; ssDNA and MB-Apt jointly capture Hg 2+ , silver nanoclusters are generated in-situ in the C-rich sequence of the ssDNA, and Hg 2+ detection is realized through electrochemical signal change of the silver nanoclusters; standard solutions with different concentrations are prepared by diluting Cd 2+ and Hg 2+ standard stock solutions, a standard curve of concentration and electrochemical signal is determined; after being pretreated, actual samples to be measured are added to the electrochemical biosensor, electrochemical signals are determined, and the concentration of the measured substance is calculated according to a linear model, so that specific, sensitive and rapid detection of the two heavy metal ions can be realized.

The application provides a use of a doxorubicin hydrochloride-DNA tetrahedron-iron-based nanoparticle complex in preparation of a drug for cancer, and belongs to the field of pharmacy. The doxorubicin hydrochloride-DNA tetrahedron-iron-based nanoparticle complex provided by the application is a complex formed by mixing doxorubicin hydrochloride, DNA tetrahedron framework nucleic acid and iron-based nanoparticles; the ratio between the doxorubicin hydrochloride, the DNA tetrahedron framework nucleic acid and the iron-based nanoparticles is (0.5-5) nmol:(5-100) μg:(0.01-1) mg. The complex shows excellent biocompatibility and effective cytotoxicity in in-vitro experiments and in-vivo experiments, can effectively inhibit the proliferation of tumor cells and promote the apoptosis of tumor cells, and has a good application prospect in preparation of anti-tumor drugs.

The invention discloses a method for preparing high-stability selenium polypeptide based on directional enzymolysis of cardamine hirsute, and relates to the technical field of polypeptide extraction.The method comprises the following steps that selenium-rich cardamine hirsute powder serves as a raw material, and high-activity selenium polypeptide D is obtained through reduction extraction, two-step enzymolysis (glutamine endonuclease and compound protease), ultrafiltration and affinity chromatography; the residues are subjected to enzymolysis and then react with selenite to generate a selenium nanoparticle compound G, D and G are mixed according to the selenium molar ratio of 1: 1-4: 1, a protective agent is added, drying is conducted, and high-stability selenium polypeptide is prepared; according to the method for preparing the high-stability selenium polypeptide based on directional enzymolysis of cardamine hirsute, low-temperature reduction extraction is combined with residue enzymolysis-nano conversion, so that the extraction conversion rate of total selenium in raw materials exceeds 95%, and waste of selenium resources is greatly reduced; the high-activity selenium polypeptide component rich in selenocystine is obtained through sequential directional enzymolysis, ultrafiltration grading and immobilized metal affinity chromatography purification, and the antioxidant activity of the high-activity selenium polypeptide component is remarkably higher than that of a conventional enzymolysis product.

The application discloses a kind of lipid nanoparticle complex and its preparation method and application, belong to molecular biology technical field.The method includes: after CdSe / ZnS quantum dot is treated by removing oleic acid ligand, with mercapto seven glycol monomethyl ether reaction, methoxyl polyethylene glycol modified water-soluble quantum dot is obtained;The water-soluble quantum dot is mixed with multi-thiol phosphonate single-stranded DNA, and DNA-quantum dot complex is formed;The DNA-quantum dot complex is mixed with complex lipid by microfluidic, and lipid nanoparticle complex is formed.The quantum dot of the application is encapsulated in the interior of lipid nanoparticle, avoids the interference of surface marker to the original property of nanoparticle, combines super-resolution imaging and single particle tracking technology, realizes real-time observation and analysis to single lipid nanoparticle transmembrane movement process under nanometer scale, and can be used for lipid nanoparticle formula screening, nucleic acid drug delivery efficiency evaluation and intracellular endocytosis mechanism research.

The application relates to the field of plant genetic engineering technology, and particularly discloses a tea tree dsRNA nanoparticle compound based on cationized bovine serum albumin and application thereof. The application takes cationized bovine serum albumin (cBSA) as a nano-carrier, forms a nanoparticle compound cBSA / dsPDS-RNA with negative charged dsPDS-RNA, and the Zeta potential of the nanoparticle compound is +21, the particle size is mainly about 12-15 nm, and the nanoparticle compound is stable. The cBSA / dsPDS-RNA nanoparticle compound is used for treating tea tree branches to obtain PDS-silenced plants. The method of the application shows that the cBSA / dsPDS-RNA nanoparticle compound can effectively silence the endogenous gene of the tea tree, and the problems of slow process, long time cost and low efficiency of traditional genetic transformation methods are solved, and a new method is provided for tea tree gene function research.

The disclosure provides compositions, methods of treatment, and methods of making and using compositions to deliver a nucleic acid to a subject that, optionally, have reduced reactogenicity and promotes a local innate immune response in the subject while promoting an adaptive immune response. Compositions described herein include nanoparticles, optionally including an inorganic particle, capable of admixing with nucleic acids encoding proteins, antibodies, or immunomodulators. Methods of using the compositions as a therapeutic vaccine for the treatment of an infection or cancer are also provided.

The invention relates to a compound, a lipid compound nanoparticle, a nucleic acid nanoparticle complex, a pharmaceutical composition and uses thereof in the drug delivery field, and belongs to the fields of biomedicine and biotechnology. The structure of the compound is shown as formula A. The compound, lipid compound nanoparticle or nucleic acid nanoparticle complex provided herein has the advantages of good biocompatibility, high transfection efficiency, low toxicity and excellent technical effects.

The present invention relates to an RNA-responsive sensor comprising a catalytic nanoparticle complex linked to a magnetic bead by a specific single-stranded DNA probe of a target RNA wherein the single-stranded DNA probe will form a duplex with the target RNA, and wherein the DNA linker is cleaved in the presence of a duplex-specific nuclease that recognizes the RNA-DNA duplex, and wherein the DNA linker is cleaved in the presence of a duplex-specific nuclease that recognizes the RNA-DNA duplex. The present invention also relates to methods of detecting RNA using a sensor and a catalytic enzyme, in which digital quantification, diagnosis and typing are achieved using a microfluidic device, to: i) release of a catalytic nanoparticle complex, and ii) release of a target RNA to form a DNA-RNA duplex with another RNA-responsive sensor.The invention also relates to methods of detecting RNA using the sensor and the catalytic enzyme.

The application belongs to the technical field of proteinuria treatment drugs, and relates to application of a lipid gold nanoparticle complex in preparation of a proteinuria treatment drug. The lipid gold nanoparticle complex is composed of a cationic liposome, a gold nanoparticle complex and a targeting peptide. The cationic liposome coats the gold nanoparticle complex, and the targeting peptide is loaded on the surface of the cationic liposome after coating the gold nanoparticle complex. The gold nanoparticle complex is a complex of a gold nanoparticle and a VE-Cadherin overexpression plasmid. The sequence of the VE-Cadherin overexpression plasmid is shown as SEQ ID NO. 1, and the sequence of the targeting peptide is shown as SEQ ID NO. 2. The lipid gold nanoparticle complex provided by the application can repair the glomerular filtration barrier, thereby improving permeability, and further achieving treatment of proteinuria.