Preparation method and application of imprinting electrochemical sensor for detecting vanillin

A technology of vanillin and sensors, applied in the detection of vanillin in food, and in the field of detection of vanillin by imprinted electrochemical method, which can solve the problems of cumbersome operation, expensive instrument, unsuitable for rapid detection, etc., and achieve good selectivity , detection speed is fast, and the effect of on-site detection is convenient

Inactive Publication Date: 2016-01-06
UNIV OF JINAN
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  • Abstract
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Problems solved by technology

The current detection methods of vanillin mainly include thin-layer chromatography, spectrophotometry, high-performance liquid chromatography, etc. These methods are

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  • Preparation method and application of imprinting electrochemical sensor for detecting vanillin

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Embodiment 1

[0028] Preparation of graphene oxide / carbon nanotubes / ionic liquid / gold nanoparticles composite (GO / CNTs / IL / AuNPs)

[0029] Graphene oxide (GO) and carbon nanotubes (CNTs) were placed in a solution of ethanol and water with a volume ratio of 1:1 and sonicated for 3 hours, and then transferred to an autoclave at 200°C for 6 hours to obtain a GO / CNTs composite; Add 15 mg of 1-butyl-3-methylimidazolium hydrobromide ionic liquid (IL) to the complex and sonicate for 30 min to obtain the GO / CNTs / IL complex; take 0.15 g GO / CNTs / IL and 3.65 mL of 0.01 mol / L chloroauric acid solution was dispersed into 100mL ultrapure water, heated to slightly boiling, then quickly added 3.0mL1% sodium citrate solution, stirred for 30min; centrifuged and washed 5 times to obtain GO / CNTs / IL / AuNPs composite material, dried .

Embodiment 2

[0031] Preparation of graphene oxide / carbon nanotubes / ionic liquid / gold nanoparticles composite (GO / CNTs / IL / AuNPs)

[0032] Graphene oxide (GO) and carbon nanotubes (CNTs) were placed in a solution of ethanol and water with a volume ratio of 1:2 and sonicated for 3 h, and then transferred to an autoclave at 150 °C for 7 h; GO / CNTs composites were obtained. Add 15 mg of 1-butyl-3-methylimidazolium hydrobromide ionic liquid (IL) to the complex and sonicate for 30 min to obtain the GO / CNTs / IL complex; take 0.15 g GO / CNTs / IL and 3.65 mL of 0.01 mol / L chloroauric acid solution was dispersed into 100mL ultrapure water, heated to slightly boiling, then quickly added 6.0mL1% sodium citrate solution, stirred for 30min; centrifuged and washed 3 times to obtain GO / CNTs / IL / AuNPs composite material, dried .

Embodiment 3

[0034] A method for preparing a molecularly imprinted electrochemical sensor for detecting vanillin, the steps are as follows:

[0035] (1) Drop 5 μL GO / CNTs / IL / AuNPs homogeneous solution onto the surface of the glassy carbon electrode and dry at room temperature;

[0036] (2) Prepare a 0.1mol / L KCl solution, add an appropriate amount of template molecules (vanillin) and functional monomers (pyrrole), and complete the pre-assembly process of template molecules and functional monomers at room temperature for 2 hours; use electropolymerization to polymerize MIPs Dry on the surface of GO / CNTs / IL / AuNPs / GCE obtained in (1) at room temperature;

[0037] (3) Use 0.05mol / L phosphate buffer solution with pH=7.0 to elute the template molecule vanillin on the surface of the sensor, and dry at room temperature;

[0038] (4) With Ag / AgCl as the reference electrode and the Pt electrode as the counter electrode, the sensor obtained in (3) combined with the template molecule

[0039] The la...

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Abstract

The invention discloses a molecular imprinting electrochemical sensor for detecting vanillin. The molecular imprinting electrochemical sensor makes the surface of a glass carbon electrode covered with a prepared graphene oxide/carbon nanometer tube/ion liquid/gold nanometer particle composite through an electrode surface decoration technology so that the aims of enhancing electric signals and improving the detection sensitivity of the sensor can be achieved. Through the electrochemical polymerization mode, the surface of the electrode is decorated with molecularly imprinted polymers with pyrrole as functional monomers and vanillin as template molecules, and the molecular imprinting electrochemical sensor is formed after the template molecules are washed away. After the template molecules are washed away, holes are formed in the surface of the sensor, and specificity matching between the holes and the structure of vanillin can be achieved. The prepared electrochemical sensor is connected to an electrochemical working station as a working electrode, current changes are produced through the specific binding of the template molecules on the surface of the electrode and the holes, and vanillin can be detected through the current changes. The prepared molecular imprinting electrochemical sensor is high in selectivity and sensitivity, easy and rapid to operate and suitable for detecting vanillin in food.

Description

technical field [0001] The invention belongs to the field of detection of nanometer materials and food additives, in particular to an imprinting electrochemical method for detection of vanillin, which can be used for detection of vanillin in food. Background technique [0002] Vanillin is a phenolic compound widely used in the food industry, such as confectionary beverages and cream products. Adding vanillin in an appropriate amount can not only maintain the ideal taste and aroma of food, but also benefit your health. However, excessive intake of vanillin can cause headaches, nausea and vomiting, and more serious cases can affect liver and kidney function. Therefore, it is meaningful to prepare a simple, sensitive and rapid sensor for the detection of vanillin. The current detection methods of vanillin mainly include thin-layer chromatography, spectrophotometry, high-performance liquid chromatography, etc. These methods are cumbersome to operate, expensive instruments, req...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 罗川南王晓娇段辉敏李剑波王延辉孙敏冯娟娟
Owner UNIV OF JINAN
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