213results about How to "Easy to detect on site" patented technology

The invention discloses an electrochemiluminescence sensor for detecting trace pesticide residue and a method for detecting pesticide residue. An electrode preparation method (figure attached) comprises the following steps: selecting an identifier of the pesticide residue; preparing quantum dots and graphene nano material according to documents; and modifying the nano material, the quantum dots and the identifier onto the electrode surface of the sensor by the electrode surface modification technology. The method for detecting the trace pesticide residue comprises the following steps: connecting the modified electrode to an electrochemiluminescence instrument and detecting the pesticide residue in the sample extract. The electrode in the invention has high specificity and sensitivity capable of reaching ng level, a basic detection process is finished only within 3-5 min, and the cost is low. The method for detecting the pesticide residue by utilizing the electrode is fast and simple to operate, and the reaction is automatically finished and the result is automatically recorded by an apparatus.

The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

The invention discloses a molecular imprinting electroluminescent sensor for detecting veterinary drug residue by taking a battery as power and a method for detecting veterinary drug residue. A preparation method of an electrode (a schematic diagram is shown in the figure) comprises the following steps of: preparing MIP (Molecularly Imprinted Polymer)s sol of the veterinary drug residue; preparing a carbon dot and preparing a graphene nano material according to documents; and modifying graphene, the carbon dot and the MIPs sol onto the surface of the electrode of the sensor. The method for detecting the trace veterinary drug residue comprises the following steps of: connecting the modified electrode to an electrogenerated chemiluminescence instrument, and detecting the veterinary drug residue in a sample extract by taking the battery as the power. The molecular imprinting electroluminescent sensor disclosed by the invention has the advantages that the specificity of the electrode is strong and the sensitivity is high and can be up to a ng grade; only 3-5 minutes are spent on a basic detection process; and the cost is low. The method for detecting the veterinary drug residue by adopting the electrode is quick and easy to operate, the reaction is automatically completed by instruments and results are automatically recorded by the instruments.

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

The invention discloses a process for preparing a trace amount mycotoxin molecular imprinted column, which comprises the following steps of: selecting a functional monomer; evenly mixing a mycotoxin template molecule, the functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent by a certain molar ratio to prepare a molecular imprinting polymer solution; preparing a nano-solution; and dressing the nano-material and a molecular imprinting polymer on the internal surface of a glass pipe column. A method for detecting the trace amount mycotoxins includes: pumping a chemiluminescence system solution and a sample solution into a chemiluminescence analyzer respectively and detecting the mycotoxins in the sample. The molecular imprinted column of the invention has advantages of high sensitivity and accuracy. The nano-enhanced trace amount mycotoxin molecular imprinted column obtained in the invention is connected to the chemiluminescence analyzer for detecting mycotoxins, and purposes of high specificity, high sensitivity and fast detection to mycotoxins in the sample can be realized.

The invention relates to an oligonucleotides chain-based mercury ion fluorescent detection chip, a manufacturing method thereof and a using method thereof. The detection chip can be in convalent binding with a T basic group on a T-enriched oligonucleotides chain specifically through Hg<2+> and mediates T-T pairing of two T-enriched oligonucleotides chains to form a stable intermolecular T-Hg Hg<2+>-T structure and thus induces the release of complementary strands which are crossed with the T-enriched oligonucleotides chain. The use of the detection chip comprises the following steps of: placing a sample to be tested on the chip, keeping a period of time, scanning the chip by using a chip signal analysis system and analyzing a fluorescent signal. The Hg<2+> detection is realized by the change of the fluorescent signal. Higher Hg<2+> concentration in the sample means the fluorescent signal weakens more. The Hg<2+> concentration range capable of being detected by the method is 1nM to 100mu M.

The invention provides an electrochemical aptamer sensor for detecting dopamine (DA). The preparation method of the electrochemical aptamer sensor comprises the following steps: modifying the prepared polyamidoamine dendrimer-gold nanoparticles (PAMAM-GNPs) compound and nickel hexacyanoferrate nanoparticles (NiHCFNPs) to the electrode surface by utilizing the electrode surface modification technology, then carrying out electro-deposition on one layer of gold nanoparticles, modifying on the electrode surface, modifying amino group to the tail end of the DA connected with an aptamer, modifying the amino group on the electrode surface by affinity of the gold nanoparticles and the amino group, and finally, combining one layer of DA to be detected outside the aptamer, thereby preparing the electrochemical aptamer sensor. The prepared electrochemical aptamer sensor used as a working electrode is connected to an electrochemical workstation, an electrochemical signal changes before and after DA connection, and the change of the electrochemical signal is utilized to detect the DA. The prepared molecularly imprinted electrochemical sensor has strong selectivity and high sensitivity, is simple and rapid to operate and is suitable for DA detection.

The invention discloses an ultrasonic leakage detector for a gas exhaust pipe for a coal mine, and relates to a gas leakage detection instrument. The ultrasonic leakage detector comprises an ultrasonic probe, a signal amplifying circuit, an audio processing circuit, a frequency display circuit and a singlechip controller, wherein the ultrasonic probe is used for acquiring leakage ultrasonic signals generated by pipe leakage; the leakage ultrasonic signals pass through the signal amplifying circuit and are input to the audio processing circuit; the audio processing circuit converts the leakage ultrasonic high-frequency signals into human eye audible low-frequency signals; and the frequency display circuit displays the frequency values of the ultrasonic signals in real time. The leakage ultrasonic signals generated by pipe leakage are acquired by adopting the ultrasonic probe, and the leakage ultrasonic high-frequency signals are converted into the low-frequency signals or the frequency values of the ultrasonic signals are displayed in real time through the frequency display circuit; and the leakage detector has low cost, is convenient to use, high in speed, accurate, reliable and convenient for field detection, and is suitable for gas leakage detection of a gas delivery pipe in the explosive gas environment under the coal mine.

The invention relates to the technical field of clinical detection, in particular to a detection reagent for the content of alcohol in saliva. The invention discloses the detection reagent for the content of the alcohol in the saliva, which is characterized in that 1.25 to 0.5U of alcohol dehydrogenase, 0.05 to 0.2mg of coenzyme NAD and 0.025 to 0.2mg of tetrazolium salt are loaded and contained in a solid phase or semi-solid phase carrier with the area of 5.0*5.0mm<2>. The detection reagent for the content of the alcohol in the saliva has high specificity, high sensitivity, and quick reaction, does not contain poisonous substances, can detect orally, is safe and reliable, shows a result without a composite sensor host, can directly contrast readings with a colorimetric card, has better thermal stability, and can be preserved for 2 years at room temperature. The reagent has the advantages of good repetitiveness of determination results, convenient carrying, simple preparation technology, low cost, easy industry promotion and convenient field detection, and can be used for the detection of drunk drivers and taken as a simple tool for postmortem examination of judicial doctors.

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the detection speed of the invention is fast, the detection time is less than 30 minutes, the degree of automation is high, and the maneuverability is good, the invention can be applicable to the locale rapid detection of health quality in the food, cosmetics, medical, environmental, and other fields.

The invention provides an aptamer electrochemical sensor for detecting malachite green (MG). A preparation method for the electrochemical sensor comprises the steps of: the prepared graphene-chitosan compound and nanogold are modified on an electrode surface, an aptamer is connected on the nanogold through chemical action, a target object is bound onto the electrode surface through the specific binding action of the aptamer and the target and then connected with an antibody of the target object, thus constructing the biosensor with a sandwich structure. When the detected solution contains the target object, a certain amount of target objects and corresponding antibodies can be fixed on the electrode surface, so that a sensing structure is formed. Horseradish peroxidase (HRP) is modified on the antibodies, and the hydrogen peroxide can be catalyzed to be decomposed, so that the change of an electrochemical signal can be generated, and the detection on the MG can be realized through the change of the electrochemical signal. The prepared aptamer electrochemical sensor is strong in selectivity, high in sensitivity, simple and fast to operate, and suitable for detection on the MG of aquatic products.

The invention relates to measurement of peroxide values, and in particular relates to an edible oil peroxide value measurement combined reagent and a detection method. The edible oil peroxide value measurement combined reagent comprises a polarity organic solvent, a reducing agent, a color development reagent and a color development stabilizing agent, wherein the polarity organic solvent comprises ethanol with the mass concentration of 237-710 g/L in percentage by mass; the reducing agent comprises ferrous sulfate with the mass concentration of 1-20 g/L in percentage by weight; the color development reagent is a sulfosalicylic acid solution with the mass concentration of 300-1,000 g/L; and the color development stabilizing agent is a pyridoxamine dihydrochloride solution with the mass concentration of 20-800 g/L. By a colorimetric analysis method, the operation is quick and simple, and interference caused by heating on a detection result is reduced; the types of the needed detection reagents is few, and the reagents are convenient to combine and carry; and the edible oil peroxide value measurement combined reagent can develop and detect a plurality of oil samples at the same time, is uniform in developing, is low in toxicity and even non-toxic, and is safe and environmentally-friendly.

The invention provides a method for detecting pesticide residues through a paper-based visualized molecular imprinting biosensor, and discloses the visualized portable microfluidic paper chip sensor which is easy to operate and low in cost and is successfully applied to on-site detection. Through the sensor, a molecular imprinting technology is successfully introduced to a microfluidic paper chip, and color changing is triggered through a catalytic effect of zinc ferrite on hydrogen peroxide and 3,3',5,5'-tetramethyl benaidine to achieve visualized detection. The sensor is constructed through the following steps that hydrophobic patterns are printed in batches, wax melting forming is performed, and the zinc ferrite is prepared and synthesized; molecular imprinting polymers are synthesized in situ; a hydrogen peroxide solution is added to trigger a reaction, and the concentration range of the pesticide residues is determined by observing the color changes on the paper chip.

The invention relates to a malachite green collaurum detection card and production and application methods thereof, belonging to the technical field of immunology. The rapid diagnosis card comprises a case and a test strip; wherein the case is opened with a sample injecting hole and an observation window, the test strip is arranged in the case, the middle part of a PVC liner plate is superposed with a nitrocellulose membrane, the two ends are respectively superposed with an absorbent pad and a sample pad, the nitrocellulose membrane is overlapped and connected with the absorbent pad and the sample pad, a membrane containing malachite green monoclonal antibody immune collaurum compound is coated on a sample region, and a detection strip containing malachite green envelop antigen and a quality control strip containing goat anti mouse IgG are arranged on the nitrocellulose membrane. The invention is a rapid, accurate and sensitive diagnosis card, requires no special apparatus, has simple operation and can meet the detection requirements of food safety, fishery and detection mechanism.

The invention provides a preparation method and an application of a current type electrochemical sensor for detecting bisphenol A, belonging to the field of a nano functional material and environmental detection. The preparation method of the bisphenol A electrochemical sensor utilizes an electrode surface modification technology to modify a prepared PtRu nano porous alloy material and a chitosan solution on the surface of an electrode, and takes the electrode which is successfully modified as a working electrode to be connected to an electrochemical work station, so as to detect the bisphenol A. The electrochemical sensor disclosed by the invention has the advantages of strong selectivity, high sensitivity, and simplicity and rapidness in operation, and is suitable for detecting the bisphenol A in a water sample.