Production method of molecular engram membrane electrode for detecting endocrine interference in trace amount surroundings and detecting method thereof

Abstract

The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.

Description

technical field The present invention relates to the technical field of environmental pollutant detection, and more specifically to a method for preparing a molecularly imprinted membrane electrode for detecting trace amounts of environmental endocrine disruptors. The present invention also relates to the use of the molecularly imprinted membrane electrode to detect trace amounts in environmental samples Approaches to Environmental Endocrine Disruptors. Background technique Endocrine Disrupting Chemicals (EDCs for short) refer to a class of foreign substances that interfere with the normal behavior of organisms and the synthesis, storage, secretion, in vivo transportation, combination, and clearance of normal hormones related to reproduction and development. Environmental endocrine disruptors are divided into the following categories: polychlorinated biphenyls, dioxins, alkylphenols, dibutyl phthalates, metals, organochlorine compounds, environmental hormones, phytoestrogens...

AI Technical Summary

Problems solved by technology

1. Uterine weight gain test and uterine calcium-binding protein CaBP-9k mRNA expression detection and protein analysis are often used in in vivo tests. This method is sensitive and specific, but its cost is high and its sensitivity is low, so it is not suitable for large-scale rapid screening. ;

2. In vitro tests mainly include E-Screen method, receptor competition test, etc. The specificity of the test itself is poor, and false positive results are prone to occur;

3. The EDCs instrument detection method established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the detection of EDCs, but this method cannot identify the structure of the analyte at one time, often Need to use GC-MS to confirm the structure, the technical cost is high, the traditional method generally takes a long time, some even up to several hours, the operation is complicated, and cannot be used for rapid on-site detection

4. For the detection and analysis of EDCs, the above methods generally have shortcomings such as low detection sensitivity, high cost, false positive results, complicated detection process, and not suitable for rapid on-site detection, so they cannot meet the needs of actual detection.

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