74results about How to "Enables high-throughput screening" patented technology

The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

The invention discloses a process for preparing a trace amount mycotoxin molecular imprinted column, which comprises the following steps of: selecting a functional monomer; evenly mixing a mycotoxin template molecule, the functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent by a certain molar ratio to prepare a molecular imprinting polymer solution; preparing a nano-solution; and dressing the nano-material and a molecular imprinting polymer on the internal surface of a glass pipe column. A method for detecting the trace amount mycotoxins includes: pumping a chemiluminescence system solution and a sample solution into a chemiluminescence analyzer respectively and detecting the mycotoxins in the sample. The molecular imprinted column of the invention has advantages of high sensitivity and accuracy. The nano-enhanced trace amount mycotoxin molecular imprinted column obtained in the invention is connected to the chemiluminescence analyzer for detecting mycotoxins, and purposes of high specificity, high sensitivity and fast detection to mycotoxins in the sample can be realized.

The invention discloses a method for rapidly screening microalgae germplasm with high grease content. The method comprises the following steps: sampling a water sample, culturing and separating dominated algae species; then, adopting an enzyme-labeling instrument to detect a light absorption value of 490nm to rapidly reflect the growth condition of algae; adopting a fat-soluble fluorescent dye Nile red to dye microalgae; utilizing 485nm exciting light and 575nm dissipating light of the fluorescent enzyme-labeling instrument to quantificationally detect the cytolipin content of the microalgae; and finally, confirming pure algal strains and storing the algae species. The screening process is simple, convenient and high-efficient and can carry out large-scale, high-efficient and rapid separation of the microalgae germplasm with high grease content and high growth rate from a natural water body while integrating the synchronous monitoring of the biomass and the grease content so that suitable algae species are separated by one step; the whole separation, screening and detection process can realize high-throughout screening without changing a culture container; the destination algal strains are obtained by one step through different detection programs, and the working efficiency of the screening is greatly improved.

The invention discloses SNP markers closely linked with a height trait of cabbage type rape, and application of the SNP markers. By using an EMS induced mutation technology, a dwarf mutant DF09 with the plant height being 65 cm is obtained. The dwarf mutant DF09 is taken as a research material, a method of map-based cloning is adopted, and a dwarf trait control locus BnDwf.C9 is subjected to finemapping in the 132 Kb interval of a C09 chromosome. In the fine mapping interval, three SNP molecule markers of BnaC09-42, BnaC09-46 and BnaC09-54 which are closely linked with a dwarf trait are obtained. According to the SNP markers closely linked with the height trait of the cabbage type rape, and application of the SNP markers, through a five primer amplification blocked mutation system technology (the BnaC09-42, the BnaC09-46 and the BnaC09-54) or a conventional PCR amplification technology (BnaC09-46pcr), a dwarf material can be accurately screened according to gene types, thus the dwarfmaterial is applied to molecular identification of early target traits, the progress of dwarf breeding is accelerated, and a foundation is laid for clone of dwarf genes.

The invention discloses an organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and a production method and application thereof, wherein an organic arsenide molecularly imprinted polymer in the aptamer-based marine product is taken as a recognition element to form a reaction layer on the surface of a gold quartz body substrate; and the organic arsenide molecularly imprinted polymer in the aptamer-based marine product is formed by polymerizing organic arsenide template molecule, aptamer functional monomer, cross-linking agent, porogen, initiating agent and organic solvent in the marine product according to the molar ratio of (0.1-2.5):3:(0.1-3):(30-60):(0.01-0.15):(1.0-10). The surface finish technology is applied to the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product, so the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product has controllability, and the sensitivity and the accuracy of the substrate are improved.

The invention discloses a drug screening method and a three-dimensional tumor slice model culture method, and relates to the field of biological medicine. The three-dimensional tumor slice model culture method is established, high-throughput screening of drugs is achieved by combining a label-free technology and/or a cell apoptosis report substance delayed imaging method, and efficient drugs for specific cancer samples are obtained within one week. Immune components of original tumors are completely reserved in three-dimensional tumor slice model culture, and which is possible that an immune checkpoint blocking test is successfully achieved through an immune checkpoint inhibitor. By means of the technology, a cheap, rapid and simple platform is provided for anti-cancer drug discovery, and accurate anti-cancer treatment is accelerated.

The invention relates to a method for preparing a glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin, which comprises the following steps: (1) selecting a functional monomer which can compound a glycosyl functional molecularly imprinted polymer with the bacterial toxin; (2) uniformly mixing a bacterial toxin template molecule, the functional monomer, a cross-linking agent, a porogenic agent, an initiating agent and an organic solvent to prepare a glycosyl functional molecularly imprinted polymer solution according to a certain molar ratio; and (3) modifying the glycosyl functional molecularly imprinted polymer on the surface of an electrode of a sensor by an electrode surface modifying technology. The method for detecting trace bacterial toxin comprises the following steps: connecting the glycosyl functional molecularly imprinted membrane electrode prepared by the method to an electrochemical workstation and detecting the bacterial toxin in an environmental sample extracting solution. The glycosyl functional bacterial toxin molecularly imprinted membrane electrode has high specificity, sensitivity and detecting sped, can realize high flux screening of a plurality of samples in short time and reduces the detecting cost.

The invention relates to a screening method of a cell strain of a GS expression system. The method includes the steps of: pressurizing methionine sulphoximine, selecting a cell pool being 25-35% in cell activity for performing monoclonal screening, and adding the cell pool to a semi-solid culture medium containing 10% of a conditional culture medium to form mono-clone (wherein final cell concentration is 150 cells/ml), and then performing screening and amplified cultivation to obtain a high-expression cell strain through a high-throughput cell screening system, and performing stability evaluation to the screened high-expression cell strain. The method can be used for obtaining the cell strain being more than 90% in stability.

The invention discloses a method for high throughput screening of microorganisms for preventing plant soil-borne fungal diseases, and belongs to the technical field of agricultural microorganisms. Themethod includes preparing the diseased oil with a high-concentration wheat root rot pathogenic bacteria culture and the soil, sowing germinated wheat seeds into the soil containing pathogenic bacteria to simulate the natural growth conditions and environment of plants and the pathogenic bacteria in the soil to the maximum extent. The method for screening for a pathogen-plant pathogenesis system of antagonistic microorganisms of the wheat bipolaris sp, the dense planting of the wheat reduces the space occupied by the screening, and the symptoms of the wheat root rot appear early, which reducesthe screening time, so that the high-throughput screening can be achieved. The screening efficiency of the microorganisms with better inhibition of pathogenic bacteria obtained in the early screeningis greatly improved, and a large number of microorganisms that cannot exert control effects in practical applications can be removed, so that the pace of microorganism screening, microorganism pesticide development and microorganism fertilizer research and development can be accelerated.

The invention provides a high-efficiency directed evolution method of a lipase gene. The method comprises the following steps: (1) constructing a display expression vector of saccharomyces cerevisiae;(2) constructing display expression recombinant plasmids of the saccharomyces cerevisiae of a lipase; (3) obtaining vector-carried lipase gene fragments in a homologous sequence and constructing an expression mutation library; and (4) analyzing the display expression mutation library and mutants. The method synchronizes the construction of the mutation library and the expression of the mutants ina general directed evolution technology and overcomes the defects that directed evolution of lipase molecules has complicated operation steps, difficult mutant selection, and the like in the prior art.

The invention provides influenza A-type influenza virus neuraminidase (NA), a gene and a preparation method thereof as well as a construction method of a screening model of an influenza virus neuraminidase inhibitor. Particularly, influenza A-type influenza virus H1N1 subtype neuraminidase is secretly expressed by pichia pastoris to obtain the neuraminidase with biological activity, and particularly, the truncated neuraminidases of 82 amino acids at N end of an NA full-length sequence is removed, so that an operation process of the NA prepared by totivirus, which is complicated, consumes time and has a safety problem, is avoided. According to the invention, the A-type influenza virus H1N1 subtype neuraminidase inhibitor screening model is also constructed by utilizing the neuraminidase; and the influenza virus H1N1 subtype neuraminidase has the advantages of definite mechanism of drug action, less material consumption, quick screening speed, high sensitivity, easy realization of high throughput screening and the like.

The invention discloses a high-producing strain containing abamectin, and also provides a screening method of the high-producing strain containing abamectin. The high-producing strain containing abamectin is (Streptomyces avermitilis) AV-185S, the preservation unit of the high-producing strain is the China general microbiological culture collection center, the preservation date of the high-producing strain is March 15, 2016. The screening method mainly comprises the steps of a, preparing a spore suspension; b, conducting ultraviolet-lithium chloride compound mutation treatment; c, preparing a streptomyces avermitilis liquid medium; d, screening the high-producing strain through liquid culture in a deep well plate; and e, detecting the strain fermentation potency. The average fermentation potency of the (Streptomyces avermitilis) AV-185S screened according to the screening method can be up to 6842.8 ug/mL.

The invention belongs to the field of analysis and detection, and relates to a preparation method of a high-throughput liquid crystal detection platform for screening an enzyme inhibitor by inducing aptamer release through enzyme catalysis. The high-throughput liquid crystal detection platform comprises the following components of: a glass slide, a copper net, liquid crystal molecules, a surfactant, aptamer coding DNA, a substrate and a substrate specific invertase. The components are utilized to modify a liquid crystal biosensor, efficient screening of the enzyme inhibitor is achieved, and the effect of the platform is better than that of an existing screening method at present. The detection method provided by the invention has the advantages of high sensitivity and short time consumption; and the preparation method has the advantages of simplicity, good selectivity, rapidness, simplicity, low cost and no need of large-scale instruments, and has a good application prospect in high-throughput screening of enzyme inhibitors.

The invention relates to a preparation method of a nano-synergistic carbohydrate functionalized molecularly imprinted column for detecting bacterial toxins, comprising the following steps: selecting a carbohydrate functional monomer which can be used for synthesizing a carbohydrate functionalized molecularly imprinted polymer with the bacterial toxins; preparing solution of the carbohydrate functionalized molecularly imprinted polymer; preparing nano solution; and modifying nano materials and the carbohydrate functionalized molecularly imprinted polymer on the inner surface of a glass tube column. A method for detecting trace bacterial toxins of the invention is characterized by connecting the carbohydrate functionalized molecularly imprinted column prepared by the above method with a chemiluminescence analyzer, respectively pumping chemiluminescence system solution and sample solution into the chemiluminescence analyzer and detecting the bacterial toxins in the samples. The preparation method of the invention has controllability and improves the sensitivity and accuracy of the column. The prepared molecularly imprinted column features strong specificity, high sensitivity and rapid detection speed, can realize high throughout screening of vast samples in short time and lower the detection cost.

The invention discloses an immune nanocrystalline quartz fluorescent sensor for field rapid in-vitro instant multi-disease synchronous diagnosis and a method for diagnosing and monitoring infectious diseases. A preparation method of a immune nanocrystalline comb multi-probe quartz plate, as shown in the figure, comprises the following steps: selecting the pathogen related protein of the infectious diseases or a marker thereof as antigen; preparing the nanocrystalline and preparing immune nanocrystalline according to the document; and modifying the immune nanocrystalline of different antibodies on the surfaces of different probes of the comb quartz plate by using the layer-by-layer surface modification technique. A multi-disease synchronous diagnosis monitoring method comprises the following steps: soaking the modified quartz plate in an environment sample solution, body fluid, blood or secretion sample; placing in a sealed quartz excitation vessel transmitting light path; and performing diagnosis monitoring on the infectious diseases. The invention has the advantages of high specificity, high sensitivity, short diagnosis monitoring time and low cost.

The invention relates to a fungi culture method for the technical field of bioengineering and biology to realize high throughput screening on strains, in particular to a moldfungi suspension culture method by high throughput screening. The method comprises the steps of selecting moldfungi, obtaining bacterial colony, setting a perforated plate culture environment, suspension culturing and the like. Through a simple mode to ensure that the bacterial colony is suspended on the surface of a liquid medium for static culturing, solves the oxygen supply problem of miniature culture, realizes high throughput culturing of the moldfungi, is associated with corresponding high throughput detecting technology to realize high throughput screening, ensures that the culturing process of the strains and the forming process of spore are combined into a whole, and further shortens the flow of strain breeding to achieve the aims of high yield strains and high throughput screening. The method has simple operation and high efficiency.

The invention discloses an antibody chip kit for screening biomarkers. The kit comprises an antibody chip, a biotin labeled cell factor detection antibody mixture and horse radish peroxidase labeled streptavidin, wherein the antibody chip comprises a bas membrane and specific antibodies of 60 cell factors fixed on the surface of the base membrane, three positive control. The kit disclosed by the invention can be used for simultaneously detecting 60 cell factors for multiple samples, overcomes the defects in the prior art of tedious operation, single detection index, low sensitivity and the like and has the advantages that the kit is cheap, convenient, sensitive, accurate, high in throughput and few in use level of specimens and can be popularized and expanded on a common laboratory and the like.

The invention discloses a micro-fluidic chip for multi-cell co-culture and a preparation method of the micro-fluidic chip. The micro-fluidic chip comprises an on-membrane cell culture layer, a porous membrane and an under-membrane cell culture layer, the cell culture layer can be provided with a plurality of cell culture channels, and each cell culture channel comprises a cell culture cavity, and a perfusion channel, an outflow channel and a cell injection port which are respectively connected with the cell culture cavity. The micro-fluidic chip disclosed by the invention realizes multi-cell co-culture, and can be used for researching the influence of drugs on different organs and tissues and the mutual action of the drugs.