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Method for preparing glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin and application thereof

A molecularly imprinted membrane and bacterial toxin technology, applied in the field of environmental pollutant detection, can solve the problems of time-consuming and laborious result accuracy, long cycle, complicated process, etc., and achieve the effect of low cost, strong specificity and high sensitivity

Inactive Publication Date: 2010-03-10
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these detection methods have high sensitivity and good stability, most of them have the disadvantages of long cycle, complicated process, time-consuming and laborious results, and low accuracy of results.

Method used

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  • Method for preparing glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin and application thereof
  • Method for preparing glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin and application thereof

Examples

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Embodiment 1

[0028] A preparation method of glycosyl functionalized molecularly imprinted membrane electrode for detecting diphtheria toxin, including the following steps:

[0029] (1) Select functional monomer A-D-pyranosylmannose that can synthesize glycosyl functionalized molecularly imprinted polymer with diphtheria toxin;

[0030] (2) The template molecule diphtheria toxin, the functional monomer AD-pyranosyl mannose, the crosslinking agent ethylene glycol dimethacrylate (EGDMA), the porogen chloroform, and the initiator azobisisobutyronitrile, The organic solvent dichloromethane molar ratio is 0.1:2.5:0.5:70:0.05:2.0 and the mixture is evenly mixed to obtain the glycosyl functionalized diphtheria toxin molecularly imprinted polymer solution;

[0031] (3) The working electrode is a glassy carbon electrode. The surface of the working electrode is polished with 0.05μm alumina powder, ultrasonically cleaned, and then 1mol / L HNO is used. 3 , 1mol / L NaOH cleaned, then thoroughly cleaned several t...

Embodiment 2

[0035] Example 2 (Tetanus Toxin)

[0036] A preparation method of glycosyl functionalized molecularly imprinted membrane electrode for detecting tetanus toxin, including the following steps:

[0037] (1) Select the functional monomer sialic ganglioside that can synthesize glycosyl functionalized molecularly imprinted polymer with tetanus toxin;

[0038] (2) Template molecule tetanus toxin, functional monomer sialic acid ganglioside, crosslinking agent trimethylolpropane trimethacrylate, porogen dichloromethane, initiator azobisisobutyronitrile, organic The molar ratio of solvent methylene chloride is 0.5:2.5:3:45:0.1:5, and the mixture is evenly mixed to obtain a glycosyl functionalized tetanus toxin molecularly imprinted polymer solution;

[0039] (3) The working electrode is a glassy carbon electrode, the electrode surface is polished with 0.05μm alumina powder, ultrasonically cleaned, and then 1mol / L HNO 3 , 1mol / L NaOH cleaned, then thoroughly cleaned several times with double dis...

Embodiment 3

[0043] Example 3 (Botulinum toxin)

[0044] A preparation method of glycosyl functionalized molecularly imprinted membrane electrode for detecting botulinum toxin, including the following steps:

[0045] (1) Select the functional monomer N-acetylglycosamine that can synthesize glycosyl functionalized molecularly imprinted polymer with botulinum toxin;

[0046] (2) Combine template molecule botulinum toxin, functional monomer N-acetylglucosamine, cross-linking agent N, N-methylenebisacrylamide, porogen methanol, initiator azobisisobutyronitrile, organic solvent The molar ratio of dichloromethane is 1:2.5:5:65:0.08:15, and the mixture is evenly mixed to obtain a glycosyl functionalized botulinum toxin molecularly imprinted polymer solution;

[0047] (3) The working electrode is a glassy carbon electrode, the electrode surface is polished with 0.05μm alumina powder, ultrasonically cleaned, and then 1mol / L HNO 3 , 1mol / L NaOH cleaned, then thoroughly cleaned several times with double dist...

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Abstract

The invention relates to a method for preparing a glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin, which comprises the following steps: (1) selecting a functional monomer which can compound a glycosyl functional molecularly imprinted polymer with the bacterial toxin; (2) uniformly mixing a bacterial toxin template molecule, the functional monomer, a cross-linking agent, a porogenic agent, an initiating agent and an organic solvent to prepare a glycosyl functional molecularly imprinted polymer solution according to a certain molar ratio; and (3) modifying the glycosyl functional molecularly imprinted polymer on the surface of an electrode of a sensor by an electrode surface modifying technology. The method for detecting trace bacterial toxin comprises the following steps: connecting the glycosyl functional molecularly imprinted membrane electrode prepared by the method to an electrochemical workstation and detecting the bacterial toxin in an environmental sample extracting solution. The glycosyl functional bacterial toxin molecularly imprinted membrane electrode has high specificity, sensitivity and detecting sped, can realize high flux screening of a plurality of samples in short time and reduces the detecting cost.

Description

Technical field [0001] The invention belongs to the technical field of environmental pollutant detection, and specifically relates to a preparation method of a glycosyl functionalized molecularly imprinted membrane electrode for detecting bacterial toxins, and the use of the glycosyl functionalized molecularly imprinted membrane electrode to detect traces in environmental samples Methods of measuring bacterial toxins. Background technique [0002] There are three main types of bacterial toxins: exotoxin, endotoxin and non-protein toxin. Exotoxin is a typical water-soluble protein. It is the most toxic bacterial toxin known to infect humans secreted by bacteria during the exponential growth phase. It has high toxicity at very low concentrations. At present, bacterial toxins have become a new generation of environmental pollutants that urgently need to be treated. Trace amounts of bacterial toxins can affect normal hormones, interfere with endocrine functions, and cause reproducti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 黄加栋于京华裴梅山汪世华葛慎光林青张秀明邢宪荣贺晓蕊朱晗孙纳新宋晓妍袁靓
Owner UNIV OF JINAN
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