Preparation method of pearl hydrolysate

A technology of pearl hydrolyzate and pearl powder, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, cosmetic preparations, etc., and can solve the problems of pearl hydrolyzate with large molecular weight, difficult to absorb, and low product yield

Active Publication Date: 2011-04-20
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the molecular weight of each component in the pearl hydrolyzate that this method gained is all bigger, is difficult for being absorbed by human body, and the yield of product is not high

Method used

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  • Preparation method of pearl hydrolysate
  • Preparation method of pearl hydrolysate
  • Preparation method of pearl hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Get the pearl powder and place it in an aqueous hydrochloric acid solution with a molar concentration of 4mol / L equivalent to 10 times its weight (liter / kg, the same below), stir until the reaction is complete, and adjust the pH value of the mixed solution with ammonia water to Neutral, centrifuged, get precipitate A;

[0041] 2) Dissolve the precipitate A with purified water, adjust its pH value to 7.5 with ammonia water, and centrifuge to obtain the precipitate B;

[0042] 3) Dissolve 1 kg of wet precipitate B in purified water, add a total of 7 grams of enzymes (wherein snail protease and papain account for 10% and 90% of the total amount of enzymes) and enzymolyze at a temperature of 55°C 4 hours, centrifuged to obtain supernatant and precipitate C;

[0043] 4) Precipitate C is dissolved with purified water, adding a total amount of 2 grams of enzyme (wherein trypsin, papain and neutral protease each account for 10%, 20% and 70% of the total amount of enzyme) at...

Embodiment 2

[0049] 1) Take the pearl powder and place it in an aqueous hydrochloric acid solution with a molar concentration of 2 mol / L equivalent to 9 times its weight, stir until the reaction is complete, adjust the pH value of the mixed solution to neutral with ammonia water, and centrifuge to obtain Precipitation A ;

[0050] 2) Dissolve the precipitate A with purified water, adjust its pH value to 8 with ammonia water, and centrifuge to obtain the precipitate B;

[0051] 3) Dissolve 1 kg of wet precipitate B in purified water, add a total of 3 grams of enzymes (wherein snail protease and papain account for 15% and 85% of the total amount of enzymes) and enzymolyze at a temperature of 52°C Centrifuge for 3 hours to obtain supernatant and precipitate C;

[0052] 4) Precipitate C is dissolved with purified water, adding a total amount of 3 grams of enzyme (wherein trypsin, papain and neutral protease each account for 25%, 15% and 60% of the total amount of enzyme) at a temperature of 55 ...

Embodiment 3

[0058] 1) Take the pearl powder and place it in an aqueous hydrochloric acid solution with a molar concentration of 2 mol / L equivalent to 8 times its weight, stir until the reaction is complete, adjust the pH value of the mixture to neutral with ammonia water, and centrifuge to obtain precipitate A ;

[0059] 2) Dissolve the precipitate A with pure water, adjust its pH value to 8.5 with ammonia water, and centrifuge to obtain the precipitate B;

[0060] 3) Dissolve 1 kg of wet precipitate B in purified water, add a total of 9 grams of enzymes (wherein snail protease and papain account for 12% and 88% of the total amount of enzymes) and enzymolyze at a temperature of 50°C Centrifuge for 2 hours to obtain supernatant and precipitate C;

[0061] 4) Precipitate C is dissolved with purified water, adding a total amount of 4 grams of enzyme (wherein trypsin, papain and neutral protease each account for 15%, 18% and 67% of the total amount of enzyme) at a temperature of 55 ° C Enzy...

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Abstract

The invention relates to a preparation method of a pearl hydrolysate. The method comprises the following steps of: (1) adding pearl powder in an aqueous solution of hydrochloric acid, stirring until the pearl powder and the aqueous solution of hydrochloric acid fully react, adjusting the pH value of the mixed solution with ammonia to be neutral and centrifugating to obtain a sediment (A); (2) dissolving the sediment (A) with purified water, adjusting the pH value of the solution with ammonia to be 7.5-8.5 and centrifugating to obtain a sediment (B); (3) dissolving the sediment (B) with purified water, adding enzyme for enzymolysis, centrifugating an enzyme solution to obtain a supernatant and a sediment (C), wherein the enzyme is the mixture of snail protease and pawpaw protease, and the addition of the enzyme is 0.3-1.05 percent by weight of the sediment (B); (4) dissolving the sediment (C) with purified water, adding enzyme for secondary enzymolysis, centrifugating and collecting a supernatant, wherein the enzyme is the mixture of the trypsinase, the pawpaw protease and neutral protease, and the addition of the enzyme is 0.2-0.45 percent by weight of the sediment (B); and (5) mixing the two supernatants, boiling and filtering with a nanofiltration membrane with the aperture of 1-3nm to obtain the pearl hydrolysate.

Description

technical field [0001] The invention relates to a method for preparing a hydrolyzate, in particular to a method for preparing a pearl hydrolyzate. Background technique [0002] Pearl is a precious Chinese herbal medicine. Pearl is formed by the nacre secreted by the mantle of the mother-of-pearl and the epidermal cells of the pearl sac. The main component of pearls is calcium carbonate, which accounts for about 82-93% of the total. In addition, pearls also contain 4-14% shell keratin protein and 2-4% water, as well as more than ten kinds of amino acids and 28 kinds of trace elements. These include anti-aging substances such as selenium and germanium. Shell keratin can be dissociated into amino acids under appropriate conditions. Amino acid is the basic unit of protein, and protein is the life basis of our body, and it is a necessary substance for regulating physiological functions and supplying heat. Li Shizhen recorded in "Compendium of Materia Medica": "(Pearl) calms co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61K8/98C12P1/00A61K35/618
Inventor 孙步祥汤凌志李霞唐丽娟陈振鹏吴柏峰
Owner GUILIN NATURAL INGREDIENTS CORP
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