Method for rapidly screening microalgae germplasm with high grease content

Abstract

The invention discloses a method for rapidly screening microalgae germplasm with high grease content. The method comprises the following steps: sampling a water sample, culturing and separating dominated algae species; then, adopting an enzyme-labeling instrument to detect a light absorption value of 490nm to rapidly reflect the growth condition of algae; adopting a fat-soluble fluorescent dye Nile red to dye microalgae; utilizing 485nm exciting light and 575nm dissipating light of the fluorescent enzyme-labeling instrument to quantificationally detect the cytolipin content of the microalgae; and finally, confirming pure algal strains and storing the algae species. The screening process is simple, convenient and high-efficient and can carry out large-scale, high-efficient and rapid separation of the microalgae germplasm with high grease content and high growth rate from a natural water body while integrating the synchronous monitoring of the biomass and the grease content so that suitable algae species are separated by one step; the whole separation, screening and detection process can realize high-throughout screening without changing a culture container; the destination algal strains are obtained by one step through different detection programs, and the working efficiency of the screening is greatly improved.

Description

technical field [0001] The invention relates to the field of renewable energy, in particular to a method for rapidly screening microalgae germplasm with high oil content, which can be used to quickly and efficiently separate and screen target algal strains from natural waters (fresh water or sea water) to required artificial culture conditions , the raw material for the production of biodiesel. Background technique [0002] With the continuous development of global economic integration, the world's energy situation is becoming increasingly tense, and in order to meet the needs of sustainable development and increasingly stringent environmental protection requirements, looking for a green and sustainable new energy source has become a concern of all countries. The development of renewable and environmentally friendly new energy sources has become one of the focuses of widespread concern in various countries, and the use of microalgae to prepare biodiesel has become a hot spot...

AI Technical Summary

Problems solved by technology

The above method to determine the fat content requires a large amount of microalgae samples and consumes a large amount of chemical preparations. The process is complicated, the error is large, and the time is long.

[0007] For the large-scale separation and screening of high-quality microalgae germplasm with high oil content, fast growth rate and suitable for industrial production, there is no efficient and systematic method at present

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