Method for rapidly screening microalgae germplasm with high grease content

A high-fat, microalgae technology, applied in microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of complex procedures, long time, and large errors.

Inactive Publication Date: 2010-01-13
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The above method to determine the fat content requires a large amount of microalgae samples and consumes a large amount of chemical preparations. The process is complicated, the error is large, and the time is long.
[000

Method used

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  • Method for rapidly screening microalgae germplasm with high grease content
  • Method for rapidly screening microalgae germplasm with high grease content
  • Method for rapidly screening microalgae germplasm with high grease content

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[0033] Below in conjunction with example the present invention is described in further detail.

[0034] The first step is the collection of natural water samples. On February 27, 2009, it was collected from the natural water in Donghu Lake, Haikou City, Hainan Province (E: 110°20'20.62"N: 20°02'14.29") with two 50ml sterile centrifuge tubes. Take about 40ml of water sample from the water body, tightly cover the tube cap and mark the clear serial number. After the two collected water samples are brought back to the laboratory, they must be filtered through a 350-mesh sieve for three times to remove plankton, and then placed in the natural environment for 24 hours.

[0035] The second step is the preparation of the medium and the setting of its concentration gradient. Prepare 50ml of each of the four liquid mediums AE1, AE2, AE3, and AE4. The formula is as follows:

[0036] 1. AE1 (1000ml)

[0037]

[0038]

[0039] 2. AE2 (1000ml)

[0040]

[0041] 3. AE3 (1000ml)

...

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Abstract

The invention discloses a method for rapidly screening microalgae germplasm with high grease content. The method comprises the following steps: sampling a water sample, culturing and separating dominated algae species; then, adopting an enzyme-labeling instrument to detect a light absorption value of 490nm to rapidly reflect the growth condition of algae; adopting a fat-soluble fluorescent dye Nile red to dye microalgae; utilizing 485nm exciting light and 575nm dissipating light of the fluorescent enzyme-labeling instrument to quantificationally detect the cytolipin content of the microalgae; and finally, confirming pure algal strains and storing the algae species. The screening process is simple, convenient and high-efficient and can carry out large-scale, high-efficient and rapid separation of the microalgae germplasm with high grease content and high growth rate from a natural water body while integrating the synchronous monitoring of the biomass and the grease content so that suitable algae species are separated by one step; the whole separation, screening and detection process can realize high-throughout screening without changing a culture container; the destination algal strains are obtained by one step through different detection programs, and the working efficiency of the screening is greatly improved.

Description

technical field [0001] The invention relates to the field of renewable energy, in particular to a method for rapidly screening microalgae germplasm with high oil content, which can be used to quickly and efficiently separate and screen target algal strains from natural waters (fresh water or sea water) to required artificial culture conditions , the raw material for the production of biodiesel. Background technique [0002] With the continuous development of global economic integration, the world's energy situation is becoming increasingly tense, and in order to meet the needs of sustainable development and increasingly stringent environmental protection requirements, looking for a green and sustainable new energy source has become a concern of all countries. The development of renewable and environmentally friendly new energy sources has become one of the focuses of widespread concern in various countries, and the use of microalgae to prepare biodiesel has become a hot spot...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/89
Inventor 邓晓东张毅费小雯
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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