37 results about "Fast Growth Rate" patented technology

The invention relates to an artificial hybridization fry breeding method for epinephelus fuscoguttatus and pink pink bass, which belongs to the technical field of distant hybridization breeding and comprises the steps of parent selection of epinephelus fuscoguttatus and pink pink bass, parent breeding, parent intensive breeding, gamete collection, artificial insemination, hatching of fertilized eggs and fry breeding. By means of reproductive regulation and distant hybridization technologies, two species belonging to two genera under the grouper family with different geographical distribution and different breeding time are hybridized, namely cross-generic hybridization under the grouper family is achieved, intergeneric reproductive isolation is broken through, and the breeding efficiency is improved. Therefore, new hybridized germplasm with excellent characteristics of high fertilization rate, high hatching rate, high survival rate, high growth speed and the like is cultivated.

The invention belongs to the technical field of rhizosphere microorganisms, and provides a strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens and application of the strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens in growth promoting and repairing of saline-alkali soil. The strain not only grows fast, but also has growth promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization. The strain is preserved in the China General Microbiological Culture Collection Center on March 11, 2025, called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the biological preservation number of the strain is CGMCC NO.33793. The SP-38 strain has multiple growth promoting functions, and through salt stress water culture tests and in-situ saline-alkali soil pot tests of corn, the strain has good effects in the aspects of improving the crop yield and repairing saline-alkali soil.

The invention discloses an Akkermansia massiliensis LTA21F2 strain and an application of the Akkermansia massiliensis LTA21F2 strain, and belongs to the technical field of microorganisms. The strain is preserved in the Guangdong Microbial Culture Collection Center on December 04, 2022, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 63035. The Akkermansia massiliensis anaerobic fermentation bacterial liquid provided by the invention is high in viable count and strong in oxygen tolerance, still has a survival rate of more than 90% after being exposed in air for 12 hours, the viable count in a sample is increased by 25.26% after being stressed by 1.5 M H2O2 for 2 hours, and the strain has a section of characteristic sequence which can be used as a specific molecular marker, and has the characteristics of high growth rate, high viable count and the like when being subjected to enlarged culture in a 200L fermentation tank.

According to the haematococcus pluvialis strain and the application thereof, cell wall breaking operation can be carried out more easily when astaxanthin is extracted, astaxanthin can be extracted more effectively, and the cost of natural astaxanthin is reduced. The haematococcus pluvialis RMC16 strain provided by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20241404. The haematococcus pluvialis mutant strain obtained by screening has the characteristics of thin wall, no secondary cell wall, high astaxanthin content and the like, the haematococcus pluvialis mutant strain is high in growth speed and can quickly reach a plateau phase, the individual proportion of parental cells in a population bred by the haematococcus pluvialis mutant strain serving as a parent is higher, the cost required by extraction of natural astaxanthin can be remarkably reduced, and the haematococcus pluvialis mutant strain is suitable for industrial production. The method is suitable for large-scale popularization.

The invention discloses a recombinant escherichia coli strain, a preparation method and application thereof, and a synthesis method of oryzanol A, and relates to the technical field of bioengineering. The recombinant escherichia coli strain takes E.coli BL21 (DE3) as a chassis cell, and is modified as follows: (a) knockout of a gene pfkA; or, the gene pfkA is knocked out, and an untranslated region of the 5'end of the gltA gene is mutated from the sequence as shown in SEQ ID NO: 1 to the sequence as shown in SEQ ID NO: 2; and (b) inserting exogenous genes agmA, agmB, agmC, agmE and agmF, so as to obtain the gene. The recombinant Escherichia coli strain is high in growth speed and short in fermentation period (within 72 hours), a culture medium which is cheap and easy to obtain can be adopted, and compared with an existing Escherichia coli system, the fermentation yield of oryzanol A is obviously increased.

The invention discloses aspergillus niger and application thereof and a preparation method of chitosan. The Aspergillus niger is Aspergillus niger WL08, the preservation number of the Aspergillus niger is CCTCC (China Center for Type Culture Collection) NO: M2025459, and the Aspergillus niger is preserved in the China Center for Type Culture Collection on March 13, 2025. The preparation method comprises the following steps: fermenting the aspergillus niger, extracting the chitin, and deacetylating the obtained chitin to obtain the chitosan. The aspergillus niger provided by the invention has the characteristics of fast thallus growth and high biomass, and further has the characteristics of fast growth speed, low fermentation cost and high chitin content in cell walls in combination with the culture medium and the culture mode provided by the invention. Meanwhile, the chitosan is produced by further combining with a fermentation mode disclosed by the invention, the extraction of the chitosan is greatly facilitated by optimizing a culture medium and fermentation conditions, and the chitosan is high in extraction rate and stable in quality, so that low-cost and efficient chitosan production is realized.

The invention belongs to the technical field of biological manufacturing, and relates to an Euglena gracilis algal strain ZC-1007 with high yield of beta-1, 3-glucan and a preparation method of the Euglena gracilis algal strain ZC-1007. The preservation number of the algal strain ZC-1007 is GDMCC (China General Microbiological Culture Collection Center) No.66479. The algal strain is high in biomass density, high in growth speed and high in beta-1, 3-glucan yield under the light polyculture condition. The preparation method of the euglena strain comprises the steps of optimizing culture conditions and efficiently screening, and obtaining the euglena strain ZC-1007 with high yield of beta-1, 3-glucan through a specific culture medium and environment control. The strain has a good application prospect in the aspect of efficiently preparing a functional component beta-1, 3-glucan.

The present invention relates to the technical field of aquaculture and breeding of seawater fishes, in particular to an industrialized cultivation method for cephalopholis sonnerati fry, comprising the steps of successively subjecting parent fishes of cephalopholis sonnerati to feed domestication and nutrient enrichment at a water temperature of 25-29°C and under a flowing water condition, so as to obtain parent fishes with fully developed gonads, etc. The present invention takes strict measures for fry quality control and infectious disease prevention and control during industrialized workshop cultivation, so as to effectively optimize the growth conditions of fry, thus increasing the growth rate and improves the health conditions and adaptability of fry while reducing the risk of diseases. By means of standardized breeding management and technical means, the growth environment and feeding management of fry can be controlled, thus effectively improving production efficiency. The cultivated cephalopholis sonnerati has the advantages of fast growth rate, high disease resistance, strong vigor, tender and delicious flesh and good market prospects.

The present invention discloses Trichoderma asperellum ZC3 and its application in the degradation of Zanthoxylum bungeanum branches. This strain has a fast growth rate and strong degradation ability. In a liquid filter paper strip culture medium, after culturing at 28 °C for 7 days, the filter paper strip completely disintegrates; in a solid culture medium of Zanthoxylum bungeanum branch powder, after culturing at 28 °C for 40 days, its lignin degradation rate for Zanthoxylum bungeanum branches reaches 78.11%, and the degradation rates of cellulose and hemicellulose reach 82.58% and 75.84% respectively. Under a scanning electron microscope, obvious fiber breakage and structural disintegration phenomena can be seen in the Zanthoxylum bungeanum branches, showing a high degradation ability. In addition, the lignin-degrading bacterium ZC3 has good tolerance to the leaching solution of Zanthoxylum bungeanum branches.

A new and distinct cultivar of Chionanthus retusus tree named ‘CRMTF’ is disclosed, characterized by a fast growth rate, prolific white flowers and dark green foliage. The new variety is easily propagated by softwood cuttings. The new variety is a Chionanthus tree, typically used for landscapes and gardens.

The application belongs to the technical field of biological manufacturing, and relates to a kind of high-yield beta-1,3-glucan fine euglena (Euglena gracilis) strain ZC-1007 and its preparation method. Euglena gracilis ​ The preservation number of the strain ZC-1007 is GDMCC No.66479. The strain has high biomass density, fast growth rate and high yield of beta-1,3-glucan under light mixed culture conditions. The preparation method of the strain includes optimization of culture conditions and high-efficiency screening. Through specific culture medium and environmental control, the high-yield beta-1,3-glucan euglena strain ZC-1007 is obtained. The strain has good application prospect in the efficient preparation of functional component beta-1,3-glucan.

The present invention discloses the application of the PdAKR2A gene in improving the photosynthetic rate and biomass of poplar, belonging to the technical field of poplar genetic engineering breeding. The present invention for the first time discloses that overexpression of the PdAKR2A gene can significantly increase the number of cambium cell layers and the width of xylem in poplar, thereby increasing the plant height and ground diameter of poplar, and thus improving the growth rate and biological yield of poplar; it can significantly increase the net photosynthetic rate, stomatal conductance, transpiration rate and chlorophyll content of poplar; it can significantly increase the lignin and cellulose contents of poplar; the provided PdAKR2A gene can be used as a candidate gene for cultivating new poplar varieties with faster growth rate and better quality, which is of great significance for promoting the sustainable development of forestry in China and alleviating the supply-demand tension in the wood market.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

This invention relates to the field of edible fungi technology, providing a method for breeding enoki mushrooms. The method includes: collecting enoki mushroom strain samples from different regions; conducting morphological observations on the collected strains; culturing the strains on standard culture media; testing the strains' resistance to diseases and pests; selecting strains with fast growth rates, high bioconversion rates, and strong resistance to diseases and pests for hybridization; screening the hybrid offspring to select individuals with round caps, straight stems, and pure white color; purifying the selected individuals through self-pollination; and expanding the cultivation of the finally selected superior strains for wider application and promotion. Through precise control of environmental parameters and strict selection criteria, enoki mushroom varieties with superior traits can be bred more efficiently and accurately, resulting in significant improvements in growth rate, morphological quality, and stress resistance.

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.

The invention discloses a strain of high-quality and high-yield sulfur gorgeous fungus and application thereof, and belongs to the technical field of microorganisms. According to the present invention, the Erioporus sulphureus 2023V48 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41575, and the preservation date is October 18, 2024, and the Erioporus sulphureus 2023V48 can be used for preparing the Erioporus sulphureus strain. The mycelial growth speed of the sulfur gorgeous fungus 2023V48 is high, culture materials can be rapidly infected, the spawn running speed is high, the fruiting body yield after fruiting is high, the content of active ingredients in mycelium fermentation liquor is high, the laccase activity is high, the sulfur gorgeous fungus 2023V48 is suitable for industrial production, the commodity performance is higher, and the application value is higher.

This invention discloses an artificial breeding method for yellow catfish, relating to the field of animal husbandry technology. The breeding method includes: (1) artificially hybridizing yellow catfish and long-snout catfish as parents to obtain a binary hybrid fish; (2) artificially hybridizing the binary hybrid fish and yellow catfish as parents to obtain a ternary hybrid yellow catfish. The breeding method of this invention, through specific selection and matching of hybrid parents, enables the excellent genetic resources of the three parents to be inherited synchronously and stably during the ternary hybridization process, thereby stably obtaining a new fish variety with fast growth rate, good stress resistance, high fish meat quality and good disease resistance, which is of great significance for ensuring the long-term supply of high-quality aquatic products and promoting the high-quality development of fisheries.

The invention relates to a denitrifying phosphorus-accumulating bacterium with a plant growth promoting function as well as a culture method and application of the denitrifying phosphorus-accumulating bacterium. The strain is (Pseudomonas sp.) DWP1; the preservation unit is CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC (China General Microbiological Culture Collection Center The strain has the advantages that the strain is high in growth speed and wide in strain carbon source utilization range, and the removal rate of NO3 <->-N in most carbon sources which can be utilized by the strain reaches 95% or above; according to the present invention, the rapid propagation of the strain is achieved under the outdoor condition, the optimal nutrient concentration of KNO3 is 1.9 g / L, the optimal nutrient concentration of sodium citrate is 2.7 g / L, and the optimal nutrient concentration of KH2PO4 is 0.58 g / L, only trace elements are required to be added, aeration is not required, the OD600 of the bacterial liquid of the strain under the concentration within 36 h can achieve 0.6, the nitrate nitrogen removal rate can achieve 98%, the TOC removal rate can achieve 100%, and the sulfate radical removal rate can achieve 66%; the strain can also be used as a plant growth-promoting bacterium to promote the growth of an aquatic plant duckweed and cooperate with plants to jointly purify a water body.

The application discloses a kind of for sheep MSTN And SOCS2 The sgRNA of gene cytosine base editing and application thereof, the sgRNA includes nucleotide such as any one or several of SEQ ID NO.1~4.This application is respectively designed sgRNA guide sequence for sheep MSTN And SOCS2 Gene, introduce stop codon in target site using CBE system, select sgRNA guide sequence and its combination from which can efficiently target sheep MSTN And SOCS2 Gene, carry out gene editing efficiency verification on cell, and successfully obtain MSTN And SOCS2 Double gene editing goat by prokaryotic embryo microinjection technology, and gene editing sheep shows muscle fiber thickening, and fast growth rate.This application establishes a set of efficient, precise double gene editing technology system, provides key breeding material and technical support for quickly cultivating muscle developed, fast growth meat sheep new variety.

The present invention relates to the technical field of plant tissue culture, and specifically discloses a method for efficiently inducing callus and regeneration of Festuca glauca. In the present invention, the seeds of Festuca glauca "Frosty" are used as explants. After disinfection and sterilization, callus induction is carried out. By inducing callus maturation, embryogenic callus is selected as the receptor, and seed disinfection and pretreatment, callus induction, subculture, differentiation culture and seedling transplantation are carried out in sequence, and embryogenic callus with light yellow hard granular shape and high differentiation rate is successfully obtained. It has the advantages of simple operation, convenient material collection, significantly higher propagation coefficient than outdoors, and fast growth rate of seedlings. The present invention obtains Festuca glauca callus and regenerated seedlings with seeds as explants for the first time, and establishes a perfect and efficient regeneration system in Festuca glauca, laying a foundation for obtaining new varieties of Festuca glauca with excellent stress resistance traits and functional verification of key genes through transgenic technology.

The invention discloses an early-maturing white flammulina velutipes strain ZJJZG003 with high amino acid content and high brittleness, an InDel marker for identification and application of the strain, and relates to the technical field of edible mushroom breeding, and the preservation number is CGMCC NO.41175. The invention further discloses a preparation method of the strain. The edible mushroom is classified and named as Flammulina filiformis, and the edible mushroom is named as Flammulina filiformis. The strain is obtained by hybridizing F24 and W1 as parents and has a relatively high growth speed, the growth speed of hyphae cultured at 30 DEG C is 5.63 mm / d, the growth speed of hyphae cultured at 25 DEG C is 4.75 mm / d far higher than the growth speed of the parents, and meanwhile, the appearance time of primordium is shortened.

The present invention relates to a method for cultivating artificial hybrid seedlings of brown-spotted grouper and red nine-spined seabass, and the method belongs to the technical field of distant hybridization breeding. The method comprises parent selection of brown-spotted grouper and red nine-spined seabass, parent cultivation, parent intensive cultivation, gamete collection, artificial insemination, hatching of fertilized eggs and seedling cultivation. The present invention hybridizes two species belonging to two genera of the grouper family with different geographical distributions and different reproduction times by means of reproductive regulation and distant hybridization technology, i.e., cross-generic hybridization within the grouper family, thereby breaking through the inter-generic reproductive isolation and cultivating new hybrid germplasm with excellent characteristics such as high fertilization rate, high hatching rate, high survival rate and fast growth rate.

The present invention discloses a Boletus aereus strain and its application. The strain PJ1208 is obtained by the method of double-single hybridization, using the protoplast monokaryotic mycelium of YL01 as the receptor and the wild strain HZ18072 as the donor. It was deposited in the China General Microbiological Culture Collection Center (CGMCC) on March 3, 2021, with the deposit number CGMCC NO. 21925. The present invention also discloses the application of the Boletus aereus strain in the production of Boletus aereus. This strain has the characteristics of genetic stability, strong vitality, fast growth rate, neat fruiting, uniform color of the fruiting body (olive brown), good mushroom shape, soft and glutinous flesh, no color change after injury, and no earthy smell. It belongs to an excellent strain and has high commercial value.

This invention discloses a SNP molecular marker linked to a major QTL locus related to the weight trait of grass carp and its application. This SNP molecular marker is located at base 13,428,777 on chromosome 24 of grass carp, where polymorphisms of both A and G alleles are observed. The SNP molecular marker screened in this invention is closely related to the major QTL locus of grass carp weight trait and plays an important role in the regulation of grass carp weight. When the genotype of this SNP molecular marker is AA, the average weight and average body length of grass carp are higher than those of individuals with genotypes AG and GG. Therefore, this SNP molecular marker can be used to assist in the selection of grass carp breeding, especially in the selection of grass carp strains with fast growth rates, and has broad application prospects.

The invention provides a formula and application of a benzopyrene degradation inorganic salt culture medium. Kefir cheese is used as a raw material, it is confirmed through a PB test that microelements, disodium hydrogen phosphate and sodium chloride in an inorganic salt culture medium serve as main influence factors, through a single factor test and a response surface test, the optimized formula of the culture medium comprises 1 g of KH2PO4, 2 g of K2HPO4, 2 g of NH4Cl, 0.2 g of MgSO4, 0.05 g of MnSO4.H2O, 0.02 g of FeCl3, 2.88 g of NaCl and 1.78 g of Na2HPO4, 12.5 mL of microelements are dissolved in 1 L of water, the degradation rate reaches 51.78%, and the microelements are dissolved in water. The culture medium has the advantages of high growth speed and low cost.

The invention discloses a jellyfish polyploid induction method and application thereof, and belongs to the technical field of marine ornamental animal breeding. According to the induction method, the polyploidy jellyfish is obtained by taking the scyphistoma stage of the jellyfish as an induction object and carrying out induction treatment through the combination of colchicine and dimethyl sulfoxide, and a plurality of polyploidy new germplasms are obtained through one-time treatment under the condition that the use amount of an inducer is small. The obtained polyploidy scyphistoma can rapidly proliferate a new individual with polyploidy characteristics through asexual propagation, and a jellyfish body with polyploidy characters such as high growth speed, long life cycle, beautiful form and enlarged individual can be obtained through transverse fissure; meanwhile, the method also has the characteristics of simple operation and low cost. Therefore, the induction method disclosed by the invention has important application value and wide popularization and application prospects.

The invention discloses a white gourd pig hybridization combination method, Yunba wild Duroc pigs obtained by hybridization through the method have higher growth speed, lower breeding cost, higher reproductive capacity and better slaughter performance, and the production efficiency of pig raising is improved.

The invention discloses a construction method of a yak fallopian tube epithelial cell immortalized cell line, and relates to the technical field of immortalized cell line construction. Two lentivirus packaging recombinant plasmids pLVX-hTERT-Puro and pLVX-SV40LT-Puro are constructed, primary yak fallopian tube epithelial cells are co-transfected, the immortalized yak fallopian tube epithelial cell line is obtained, the cell line has the advantages of being high in growth speed, high in activity, stable in form and capable of being subcultured infinitely in vitro, the cell line has the cancerization-free characteristic, and the cell line can be used for preparing an immortalized yak fallopian tube epithelial cell line. Immortalized genes can be stably expressed, the number of chromosomes is consistent with that of primary cells, and hormone receptors of estrogen and progestational hormone are normally expressed. Therefore, the cell line constructed by the construction method of the yak fallopian tube epithelial cell immortalized cell line is safe and reliable, and a safe and reliable in-vitro model is provided for researching related problems in the field of yak breeding.

The present invention provides a molecular marker of an rrp44 gene SNP locus related to the growth of channel catfish, and a detection method and application thereof, and designs primers covering rrp44 the entire gene region and obtains 20 haplotype SNP molecular markers for screening fast-growing channel catfish individuals. Individuals with genotypes A / A and G / G are screened as parents to produce individuals with the A / G haplotype. The method of the present invention is simple to operate and rapid in detection, improves the accuracy of screening channel catfish parents, and can quickly obtain channel catfish parents with fast growth rate and genetic stability.

The present invention discloses a strain of Bacillus acidiceler CNBG-PGPR-17 and its application; the strain was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on October 28, 2021, with a deposit number of CGMCC No. 23673, and is classified as Bacillus acidiceler. The strain has a fast growth rate, good stability, and is easy to scale up and culture; it has a strong ability to dissolve inorganic phosphorus, and the amount of inorganic phosphorus dissolved can reach 277.8 mg / kg after 7 days of inoculation; it can secrete small molecule organic acids such as lactic acid and succinic acid, and reduce the pH value of the solution; it can increase the content of soil organic matter, total nitrogen, available phosphorus and available potassium, and reduce the pH value of the soil; it promotes the growth of blueberry seedlings and improves the quality of blueberries.