Constructing method for transgene Chlamydomonas reinhardtii bioreactor

Abstract

The invention relates the construct method for transgenosis rhinestone algae bioreactor. The method comprises the following steps: using the rhinestone algae as transgenosis receptor biology, highly effective expressing the carrier construct by constructing the exogenesis gene containing screening mark expression frame, leading the destination genes in rhinestone algae with bead milling method, electric punching method and other methods, integrating them into nucleus genome or chloroplast genome, and adopting a series of gene expression regulate and control technology to construct the transgenosis rhinestone algae bioreactor. The transgenosis rhinestone algae bioreactor possesses the characters of fast growth rate, short breeding cycle, easy segregation, photoautotroph and large scale culture, fast and cheap producing medicinal protein, industrial raw materials, and feeding vaccination, and possessing the bioactivity secondary metabolite.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for constructing a transgenic bioreactor, in particular, using Chlamydomonas reinhardtii to construct a transgenic algae bioreactor, which can realize rapid and cheap production of various medicinal proteins, edible vaccines, and biological Active secondary metabolites, industrial raw materials, etc. Background technique [0002] Recombinant DNA technology was developed in the Escherichia coli expression system in the 1970s. With the continuous development and improvement of modern molecular biology technology, the high-efficiency expression of exogenous genes has become an important biotechnology means for genetic engineering pharmaceuticals, gene diagnosis and treatment, modern agriculture and industrial raw material production. It has become a hot issue in modern biotechnology to research and develop various high-efficiency expression systems of exogenous genes that meet d...

AI Technical Summary

Problems solved by technology

[0003] Although several mature exogenous gene expression systems have their own advantages, there are still various shortcomings: although the E. coli expression system has a large amount of expression, most of the products exist in the form of inclusion bodies and cannot be glycosylated Modification; the yeast expression system solves the problem of glycosylation of eukaryotic gene products, but there are also problems such as the toxicity of formaldehyde inducers, the accumulation of product ethanol and the high cost of fermentation substrates; the higher plant expression system can use plant photosynthesis products as Substrates for the production of foreign proteins, but the production cycle is long and takes up a lot of space

There has also been a report of using Chlamydomonas reinhardtii to express foreign genes in China (zhang et al, NS 3-C Chimeric Antigen Gene of Hepatitis C Virus was Introduced Site-specifically into Chloroplast Genome of Chlamydomonas reinhardtii, Hereditas, 1999, 21 (6) 1- 6; wang et al, Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii, Chinese Science Bulletin, 2004, 49 (16) 1713-1717), but the expression level needs to be improved, and a complete high-efficiency expression system for exogenous genes has not been established

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