Lactobacillus salivarius separated from pig intestinal tract and used for producing fermented feed

A technology of Lactobacillus salivarius and fermented feed, applied in animal feed, animal feed, bacteria, etc., can solve the problems of short shelf life, poor resistance, unstable application effect, etc.

Inactive Publication Date: 2017-03-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, people have found in actual production that some microecological products have unstable application effects in ac

Method used

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  • Lactobacillus salivarius separated from pig intestinal tract and used for producing fermented feed
  • Lactobacillus salivarius separated from pig intestinal tract and used for producing fermented feed
  • Lactobacillus salivarius separated from pig intestinal tract and used for producing fermented feed

Examples

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example 1

[0012] Example 1, the acquisition of pig source Lactobacillus strain

[0013] 1.1 Isolation, screening and identification of Lactobacillus salivarius from pigs

[0014] The fresh feces of large white × landrace binary hybrid sows were diluted to 10 with normal saline gradient in the laboratory. -4 -10 -6 , then smeared on MRS agar medium, cultured at 37°C for 24-36h, and when a single colony appeared, pick the colony for further purification by streaking, purify the strain 2-3 times, and store the obtained culture in a refrigerator at 4°C , for morphological observation and biochemical identification.

[0015] Take a clean slide, use an inoculation loop to take a ring of sterile normal saline on the slide, pick a small amount of bacteria, spread evenly on the slide, dry, fix, then stain, and examine under the microscope (16×100). Bacterial smears with clear vision and typical morphology were picked, and the morphology and purity of the bacteria were observed with a light mi...

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Abstract

The invention relates to separation and screening of lactobacillus salivarius in the pig intestinal tract and biologically fermented feed produced by lactobacillus salivarius as an inoculant and belongs to the field of high quality strain screening and solid feed. According to self-characteristics, microorganisms such as lactic acid bacteria produce a large amount of organic acid such as lactic acid through fermentation under anaerobic conditions so that feed pH is reduced and thus the number of lactic acid bacteria is increased and harmful bacteria in the feed are reduced. The lactobacillus salivarius is isolated from the healthy pig intestinal tract and has the characteristics of fast growth rate and high lactic acid yield. The fermented feed produced by inoculating complete feed or a feed raw material with the lactobacillus salivarius is environmentally friendly and safe, has a thick sour fragrance and is well received by animals.

Description

technical field [0001] The present invention relates to the screening of high-quality lactic acid bacteria and microbial fermented feed technology, in particular to the isolation and screening of a strain of Lactobacillus salivarius isolated from the intestinal tract of pigs that can be used to produce fermented feed, and the fermented feed produced by inoculating it with complete feed or feed raw materials. feed. [0002] technical background [0003] Since the 1950s, feed antibiotics have been used on a large scale in aquaculture production, and they have played a huge role in disease resistance, disease prevention, and promotion of animal growth. However, with the development of intensive production in the aquaculture industry, microbial resistance brought about by the addition of feed antibiotics has been continuously enhanced and the negative impact of drug residues in agricultural products has gradually become prominent. Many developed countries in the world have announ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/225
Inventor 毛胜勇高擎燏朱伟云黄瑞华
Owner NANJING AGRICULTURAL UNIVERSITY
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