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A strain of Lactobacillus salivarius and its application

A technology of Lactobacillus salivarius and lactic acid bacteria, applied in the field of microorganisms, can solve the problems of incomplete restoration of tooth status, achieve the effect of preventing dental caries, important application value, and inhibiting streptococci

Inactive Publication Date: 2019-12-03
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most common treatment for dental caries is filling surgery. However, this treatment method can only be used as a restoration method and cannot completely restore the state of the tooth itself. Therefore, the key to caries treatment is early prevention

Method used

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  • A strain of Lactobacillus salivarius and its application
  • A strain of Lactobacillus salivarius and its application
  • A strain of Lactobacillus salivarius and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, isolation and identification of Lactobacillus salivarius

[0068] 1. Separation

[0069] The saliva of three preschool children (all volunteers who gave informed consent to the test content) was taken, and evenly spread on the MRS solid plate, and then placed in a carbon dioxide incubator for static culture at 37°C. After culturing until the 2nd day, a single colony was produced. Pick a single colony and repeat purification more than three times. The three strains of bacteria isolated were named Bacteria LJ, Bacteria A and Bacteria B, respectively.

[0070] 2. Identification

[0071] 1. Morphological identification

[0072] The bacteria (bacteria LJ, bacteria A or bacteria B) isolated in step 1 were inoculated on the MRS solid plate, then placed in a carbon dioxide incubator, cultured at 37°C, and the morphology of a single colony was observed after 2 days.

[0073] Bacteria LJ, Bacteria A, and Bacteria B were identified as Gram-positive bacteria, sho...

Embodiment 2

[0082] Embodiment 2, the inhibitory effect of Lactobacillus salivarius to Streptococcus mutans

[0083] The experiment was repeated three times, and the average value was taken. The specific steps of each experiment are as follows:

[0084] 1. Inoculate a single colony of Lactobacillus salivarius LJ into a test tube containing 10 mL of MRS liquid medium, and then place it in a carbon dioxide incubator. After static culture at 37°C for 24 hours, the bacterial liquid of Lactobacillus salivarius LJ was obtained (OD 600nm value around 0.5).

[0085] According to the above method, the single colony of Lactobacillus salivarius LJ is replaced by the single colony of Lactobacillus salivarius A, and other steps are all unchanged, and the bacterium liquid of Lactobacillus salivarius A (OD 600nm value around 0.5).

[0086] According to the above method, the single colony of Lactobacillus salivarius LJ is replaced by the single colony of Lactobacillus salivarius B, and other steps are...

Embodiment 3

[0101] Embodiment 3, the experimental effect of Lactobacillus salivarius LJ on each bacterial genus in the oral flora

[0102] The experiments in this example were all carried out under the premise of the informed consent of the volunteers, and all signed informed consent forms. There are a variety of harmful bacteria in the oral cavity. The names of some harmful bacteria and their relationship with diseases are shown in Table 3.

[0103] table 3

[0104] Harmful bacteria name relationship with disease Streptococcus mutans cause dental caries Prevotella It is related to the occurrence and development of dental caries, periodontal disease and pulpitis Facultative diplococci infective endocarditis Actinomycetes Related to the occurrence and development of dental caries and periodontal disease ciliate fungus Root granuloma and ciliate mycotic sepsis

[0105] 1. Preparation of tablets

[0106] 1. Preparation of Tablet A

[0107...

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Abstract

The invention discloses a strain of Lactobacillus salivarius and its application. The Lactobacillus salivarius provided by the present invention is Lactobacillus salivarius LJ, and its preservation number in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No.12857. Experiments have proved that Lactobacillus salivarius LJ provided by the present invention plays a crucial role in preventing dental caries and / or inhibiting streptococcus and / or reducing harmful bacteria in the oral cavity and / or increasing beneficial bacteria in the oral cavity, and has important application value .

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Lactobacillus salivarius and its application. Background technique [0002] Lactobacillus is a recognized probiotic, which has broad-spectrum antibacterial properties and has a certain inhibitory effect on common harmful bacteria. Lactobacillus salivarius is a member of the Lactobacillus family and often exists in the digestive tract (such as the oral cavity) of the human body. [0003] Caries is a common disease that endangers human oral health. Its secondary infection can form pulpitis and periapical periodontitis, and even cause alveolar bone and jaw inflammation. At present, the most common treatment for dental caries is filling surgery. However, this treatment method can only be used as a restoration method, and cannot completely restore the state of the tooth itself. Therefore, the key to caries treatment is early prevention. Caries is a bacterial disease, the ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/02A61P31/04C12R1/225
CPCA61K2035/115C12N1/20C12N1/205C12R2001/225
Inventor 黄留玉黄思妺刘威邹大阳杨展董德荣刘宁伟
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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