249 results about "Sodium glycerophosphate" patented technology

The invention relates to chitosan double-network quick response-type injectable hydrogel and a preparation method thereof. The injectable hydrogel is prepared from chitosan or derivatives of the chitosan and sodium glycerophosphate serving as raw materials, and dialdehyde compounds or diepoxy compounds serving as crosslinking agents, wherein the mass ratio of the chitosan to the sodium glycerophosphate is 1:(0.5-10); the mass ratio of the crosslinking agents to the chitosan is (0.01-1):1. The method comprises the following steps: evenly mixing a hydrochloric acid aqueous solution of the chitosan with a sodium glycerophosphate solution in the environment at -1 to 5 DEG C, so as to obtain a solution of which the pH is 7-7.2, and evenly mixing the solution with a crosslinking solution at 35-37 DEG C. Transformation from a sol state to a gel state can be quickly finished under a specific condition, the process is irreversible, and the transition time is controllable. The chitosan double-network quick response-type injectable hydrogel has the advantages of simple reaction condition, short response time, good thermal stability of gel, high mechanical strength, good biocompatibility and the like. The chitosan double-network quick response-type injectable hydrogel is applied to the fields such as tissue filling and repairing, biological scaffolds, scar resistance and anti-adhesion, biogel, and quickly molding or three-dimensional printing materials and the like.

The invention discloses a method for preparing injectable chitosan/sodium glycero-phosphate/collagen hydrogel, belonging to the field of biotechnology and tissue engineering; the invention particularly relates to a technology for preparing temperature sensitive type injectable material. The method is characterized in that 2.2% (W/V) of chitosan solution is mixed with 50% (W/V) of beta-sodium glycero-phosphate solution at low temperature to prepare chitosan/sodium glycero-phosphate solution; then, the solution is mixed with collagen solution following a volume ratio of 1:10-10:1 at low temperature; the mixture is retained in the liquid state at room temperature( 8 DEG C) and is turned into gel at body temperature within 12min. The invention has the beneficial effects that the chitosan/sodium glycero-phosphate/ collagen hydrogel can be maintained in the liquid state at low temperature when pH value is within physiological range (pH value ranges from 7.0 to 7.2); the mixture is turned into gel when the temperature is raised to body temperature (37 DEG C). As ideal cell growth substrate collagen is added, the hydrogel features fine biocompatibility.

The invention relates to a collagen temperature-sensitive hydrogel and a preparation method thereof Soft tissue filling materials utilized in the existing plastic and beauty treatment industry have the problems of hardening, production of phyma, too fast absorption speed, and induction of chronic inflammation. American F Dalton approves successively multiple products for soft tissue filling, and main components of the products comprise bovine collagen, hyaluronic acid sodium, polymethyl methacrylate (PMMA) globules and the like. The products have good effects but can still produce cases of redness and allergy of a used position and too fast absorption. In the invention, collagen solution, chitosan acid or hyaluronic acid, and beta-sodium glycerophosphate solution are mixed according to a volume ratio of (1 to 3): (1 to 5): (1 to 2), then are stirred well at a temperature of 0 to 15 DEG C for 10 to 30 minutes and are adjusted to a pH of 7.4 to form temperature-sensitive hydrogel. The temperature-sensitive hydrogel has the advantages of good gel forming property at a temperature of 37 DEG C, gel forming time within 10 minutes, good mechanical strength, long degradation time, low immunological rejection, and favorable safety and effectiveness.

The invention discloses an injectable hollow hydroxyapatite microsphere/chitosan composite drug carrier material. The material comprises the following components in percentage by weight: 0.5-2wt% of hollow hydroxyapatite, 2wt% of chitosan, 5.6wt% of beta-sodium glycerophosphate and 90.4-91.9 wt% of water. The preparation method comprises the following steps: first, loading drug in the hollow sodium hydroxyapatite microsphere; then, preparing chitosan thermo-sensitive hydrogel; finally, mixing the drug-loaded hollow hydroxyapatite microsphere and the chitosan thermo-sensitive hydrogel in a solid-liquid ratio of (0.05-0.2)g/10ml, and stirring to obtain the injectable hollow hydroxyapatite microsphere/chitosan composite drug carrier material. The material can realize slow and controlled release of drug, gives the material injectable molding property, and realizse minimally invasive therapy to bone defect or fracture.

The invention belongs to the field of tissue engineering and discloses chemically-crosslinked natural polysaccharide-based injectable hydrogel, a preparation method thereof and application thereof in tissue engineering materials, especially in conjunctival repair. The preparation method includes the following steps: adding an acrylated natural polysaccharide material water solution into a sulfhydrylated natural polysaccharide material water solution, mixing well, adding a beta-sodium glycerophosphate solution to adjust pH to be neutral, and incubating at temperature of 37 DEG C to obtain the chemically-crosslinked natural polysaccharide-based injectable hydrogel. The hydrogel is controllable in performance, can realize gelation under human physiological conditions, is needless of adding other chemical crosslinking agents, can be used as a tissue engineering material and is especially suitable for conjunctival repair, injectable, quick in reaction, capable of realizing gelation within 5-15min, in-situ in forming, high in surgical operability and automatic in bonding in the process of surgery, stitching for fixing is not needed, and wound surfaces in any shape and at any position can be protected effectively.

The present invention discloses a quick-concreting composite material of calcium phosphate solid cement, comprising a solid phasing part and a liquid phasing part; the preparation method of the composite material is that: preparing Alpha-TCP solid cement powder with solid-state sintering; forcing the Alpha-TCP cement powder and the carbon nanotube via the ball mill, and then acquiring the solid cement composite powder; putting the different quantity of chitosan and Beta-sodium glycero-phosphate respectively into the dilute hydrochloric acid and deionized water, obtaining the mixing liquid of solid cement; putting the solid cement composite powder and the hydroxyapatite seed into the mixing liquid of solid cement; evenly mixing the solid cement slurry and then pouring into the mould to be exhausted and planned; acquiring quick-concreting composite material of calcium phosphate solid cement. The present invention provides a quick-concreting composite material of calcium phosphate solid cement which has great mechanical property, short concreting time, certain bioelectromagnetic effects and good biocompatibility, and the preparation method.

The invention discloses injectable compound bone cement and a preparation method thereof. The invention further discloses a kit for preparing the injectable compound bone cement. The kit comprises solid powder and a curing liquid, wherein the solid powder comprises calcium phosphate bone cement powder and chitosan microspheres; and the curing liquid contains glycerophosphate. The injectable compound bone cement is prepared by uniformly mixing the solid power with the curing liquid, has superior scattering resistance, superior injection performance, rapid self-curing performance, high compressive strength and high biocompatibility, and can be used for preparing a biomedical material applied to bone defect reconstruction, bone fracture treatment and bone repair.

The invention relates to a preparation method and an application of a bioactive factor loaded temperature-sensitive composite gel carrier. The method comprises the following steps: 1. preparing a sodium alginate hydrosol; 2. preparing a sodium alginate-chitosan hydrosol through the hydrosol prepared by the step 1; 3. preparing a sodium glycerophosphate solution, adding the sodium alginate-chitosan hydrosol under a stirring condition to obtain a uniform sol; 4. adding adiponectin to the uniform sol prepared by the step 3, adding Ca<2+> and uniformly stirring; and 5. adding calcined bone powder to the sol prepared by the step 4 and uniformly mixing to obtain the bioactive factor loaded temperature-sensitive composite gel carrier. The preparation method, for shortcomings of the prior art of repairing missing and defects of an extraction socket wall, can greatly improve bioactivity, biocompatibility, mechanical tolerance and a slow release performance of a normal gel carrier, thus providing a novel repairing material for treatment of repairing missing and defects of the extraction socket wall.

The invention provides a kit and method for inducing differentiation from bone mesenchymal stem cells to osteoblasts. The kit comprises an osteoblast inducing culture solution and an osteoblast identification solution, wherein the inducing culture solution comprises a cell culture solution A, a cell culture solution B, a cell culture solution C, a cell culture solution D and a cell culture solution E; the osteoblast identification solution comprises a cell immobilizing solution, coloring agents and a detergent; the cell culture solution A is an alpha-minimum essential medium (MEM) liquid culture medium; the cell culture solution B is fetal bovine serum; the cell culture solution C is a dexamethasone solution; the cell culture solution D is a beta-sodium glycerophosphate solution; the cell culture solution E is a vitamin C solution; the cell immobilizing solution is 4% paraformaldehyde; the coloring agents include sodium thiosulfate, silver nitrate and neutral red; and the detergent is double distilled water.

A synergistic composition, or the use of that composition in the manufacture of a medicament, or a method of treatment including the use of that composition, for the treatment of hair loss and baldness, for combined, sequential or simultaneous administration, in any form, via any biological route. In its optimal embodiment the composition consists, in the form of a lotion: 1600-2400 IU/mL Vitamin A Palmitate, 0.64%-0.96% Thiamine Hydrochloride, 0.64%-0.96% Pyridoxine Hydrochloride, 4.8%-7.2% Niacinamide, 2.85%-5.2% D-Panthenol, 1.6%-2.4% L-Arginine, 3.6%-4.4% Methyl Sulphonyl Methane (MSM), 0.08%-0.12% Ginger Oil, 0.08%-0.12% Cinnamon Oil, 0.0996%-0.1494% Oleoresin Capsicum, 1.3%-1.95% Magnesium, 2.4%-3.6% Zinc, 0.192%-0.288% Manganese, 2.6%-3.9% Urea, 2.4%-3.6% Sodium Glycerophosphate, 4.8%-7.2% L-Lysine HCl, plus Preservatives, Co-solvent (Propylene Glycol), Fragrances, Anti-Oxidant, Cooling agent (Menthol), Emulsifier, and Vehicle (Purified water).

The invention discloses a preparation method of injectable chitosan grafted cyclodextrin compound drug-carrying hydrogel. The preparation method comprises the steps of carrying out amination and hydroformylation on cyclodextrin, reacting by virtue of hydroformylated cyclodextrin with chitosan so as to obtain chitosan grafted cyclodextrin, soaking molecules of obtained chitosan grafted cyclodextrin into medical liquid, uniformly stirring, filtering, carrying out freeze drying so as to obtain drug-carrying chitosan grafted cyclodextrin, adding drug-carrying chitosan grafted cyclodextrin into a chitosan water solution, adding beta-sodium glycerophosphate, so as to obtain the injectable chitosan grafted cyclodextrin compound drug-carrying hydrogel. The preparation method has the beneficial effects that reaction conditions are mild, and the operation is relatively simple and convenient; the stability of a drug concentration in a gel system of the prepared hydrogel can be maintained for a long time, and a drug can be effectively and controllably released for a long time, so that the utilization rate of the drug is increased; and the preparation method has relatively wide application prospects.

The invention relates to a temperature-sensitive in-situ gel preparation composition for intra-articular injection and a preparation method thereof, and belongs to the field of medicine preparations. The preparation method comprises the following steps: preparing diclofenac sodium-carrying sodium alginate microspheres by combining a microsphere technology with a temperature-sensitive gel technology, and then carrying the medicine-carrying microspheres in a chitosan/beta-sodium glycerophosphate temperature-sensitive in-situ gel system to obtain a diclofenac sodium in-situ gel preparation composition. The composition is in a liquid state at a room temperature, capable of being converted to a semi-solid gel at a body temperature and forming a medicine storeroom at a medication part after being partially injected to enter an articular cavity, and beneficial to slowly releasing the medicine, prolonging the time of staying at the injection part, of the medicine, and enhancing the efficacy. The composition disclosed by the invention can be used for treating arthritis diseases of rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and the like, as well as applicable to both diclofenac sodium and other arthritis treatment medicines of non-steroidal anti-inflammatory medicines, steroid hormone medicines, biological preparations and the like.

The invention relates to the technical field of stem cells, in particular to a placenta-derived mesenchymal stem cell freezing medium. The placenta-derived mesenchymal stem cell freezing medium comprises the following components in parts by weight: 40-70 parts of DEME culture medium, 2-6 parts of sodium glycerophosphate solution, 1-5 parts of hydroxyethyl starch, 1-4 parts of mannitol solution, 0.3-0.8 part of ethanediol, 0.5-2 parts of polyvinyl alcohol, 0.5-1.8 parts of trehalose, and 0.5-1.5 parts of polyvinylpyrrolidone. With the adoption of the freezing medium for storing placenta-derived mesenchymal stem cells, the damage on the stem cells in the freezing process can be reduced, and the survival rate of the stem cells after thawing is increased.

The invention discloses a bone tissue engineering scaffold material and a preparation method thereof. The preparation method for the bone tissue engineering scaffold material comprises the steps that firstly, fibroin / hydroxyapatite powders are synthesized according to a bionic principle, the fibroin/ hydroxyapatite powders are dissolved in sericin solution, then the sericin solution (containing the fibroin/ hydroxyapatite powders), chitosan solution and sodium glycerophosphate solution are mixed, the mixed solution is turned into hydrogel by controlling the temperature, the hydrogel is dried through prefreezing, unfreezing, refreezing and final freezing, and the bone tissue engineering scaffold material is obtained. The bone tissue engineering scaffold material has the advantages that the mechanical property is good, the biocompatibility is good, the degradability and formability matching with osteocyte growing feature are good, and the osteocyte can be induced to grow into the material; the physiological process of the ingrowth of the osteocyte is accelerated, the performance to promote the growth of the osteocyte is achieved, the recovery time of a patient can be shortened, the clinic treatment process is accelerated, and the pain of the patient is decreased. The preparation method has the advantages of being mild in reaction condition, simple in technique, low in cost and suitable for amplifying production.

The invention provides recombinant collagen and thermo-sensitive hydrogel of the recombinant collagen. The invention further provides a preparation method of the thermo-sensitive hydrogel, comprisingthe following step: standing chitosan, collagen, carbodiimide, N-hydroxysuccinimide, beta-sodium glycerophosphate and a sodium bicarbonate solution at the temperature of 37 DEG C.

The invention discloses a preparation method of an injectable hydrogel with a photo-thermal property, and belongs to the field of functional materials. In a malignant tumor treatment, a patient shouldtake medicines for many times, while provided hydrogel only needs one injection. The preparation method comprises the following steps: at first, preparing a dopamine modified sodium alginate bio-macromolecule (Alg-DA); adjusting the gelation temperature of a temperature sensitive hydrogel (CGP) of chitosan (CS) and sodium beta-glycerophosphate (beta-GP) to the range of human physiological temperature so that the hydrogel is in a sol state at a room temperature and will become gel after arriving at a human body; then mixing Alg-DA and CGP to obtain an injectable temperature sensitive hydrogel,and doping a polydopamine wrapped gold nano rod (Au-PDA NRs), which is prepared in advance, into the hydrogel to obtain the injectable hydrogel with a photo-thermal property (CGP/Alg-DA@Au-PDA). Thehydrogel can fix Au NRs in tumors and carries out multiple photo-thermal conversions under laser radiation so as to treat tumors by utilizing high temperature. The injectable hydrogel has a potentialapplication value in the biomedicine field.

The invention relates to a method for preparing biologically active titanium materials, which is characterized in that the method includes the steps as follows: (1) metal titanium or titanium alloy powder and the like of 5 microns to 100 microns are pressed statically for shaping and then sintered in vacuum at the temperature of 1100 DEG C to 1300 DEG C; (2) microarc oxidation treatment is conducted on the materials after powder matallurgy is sintered in electrolytic aqueous solution prepared by calcium acetate, natrium glycerophosphoricum and hydroxide to obtain the biologically active titanium materials. The obtained titanium materials contain pores internally; the surface of the titanium material shows the complex porous state and has two states of the pores, one is microarc oxidation pore and the other one is large pore formed by the powder metallurgy with the microarc oxidation pore on the inside wall. The biological activation and biomechanical compatibility of the surface of the obtained titanium materials are higher than those of the titanium materials obtained by the single powder metallurgy method. The preparing method of the invention can be used for preparing the biological replacement (recovery) body for hard tissues such as human bones, teeth, etc.

The invention discloses a medical antibacterial hydrogel dressing and a preparation method thereof. The hydrogel dressing is prepared from the following raw materials: thiolated chitosan, sodium glycerophosphate hydrate, carbomer, oxidized sodium alginate, L-arginine ethyl ester dihydrochloride, theophylline, glycine fumarate, L-carnitine powder, stachyose, hydroxyethyl methylacrylate, tri(propylene glycol) diacrylate, ginkgo flavonol, polyhexamethylene biguanidine, povidone iodine, alcoholic extract of herba andrographitis, triethanolamine and de-ionized water. The hydrogel dressing prepared by the invention can achieve inhibitory rates 91% and 94% or above on pseudomonas aeruginosa and staphylococcus aureus separately, and the hydrogel dressing has a broad-spectrum antibacterial property and a durable antibacterial effect; and in addition, the hemostasis time of the dressing is just 9-18s and healing time is shorter than 10d, and healed regenerated skin has a smooth and delicate surface; therefore, the hydrogel dressing is excellent in wound healing capacity and quite good in healing effect.

The invention discloses injectable chitosan/keratin composite hydrogel capable of promoting myocardium repair and a preparation method of the hydrogel. The hydrogel consists of four solutions, namely chitosan, sodium beta-glycerophosphate, genipin and keratin according to a volume ratio of 10: (1-5): (1-3): (0.1-0.5). The preparation method comprises the following steps: 1) extracting keratin from human fairs by using peracetic acid and Tris alkaline; 2) adjusting the pH value of a water-soluble chitosan solution by using sodium beta-glycerophosphate so as to be close to a microenvironment in a human body; 3) introducing genipin as a crosslinking agent, and controlling the gelling time of a hydrogel solution by changing the use amount of the genipin so as to achieve the purpose that the mixed solution can be rapidly gelled in situ after being injected into an organism. Experiments prove that the composite hydrogel has favorable biocompatibility and has the capability of remarkably promoting the growth of myocardial cells; the hydrogel is wide in sources of materials and low in cost and has a broad application prospect in the field of tissue engineering repair.

The invention provides a hydrogel mask matrix and a preparing method thereof. The hydrogel mask matrix is prepared from, by mass, 2% of sodium alginate water solution, 56% of a sodium glycerophosphate water solution, 5% of a sodium carboxymethylcellulose water solution, 8% of a polyvinyl alcohol water solution and 2% of a chitosan solution, the raw materials are mixed uniformly, poured into a mold, cured in a water bath and subjected to multigelation for 2-4 times. The matrix raw material components are biomacromolecules and other natural and harmless components good in biocompatibility and skin affinity, safe and harmless to the human body, and ensure that the mask matrix is free of sensitization; the prepared matrix material is uniform, good in glossiness and good in peeling integrity, has fine jade texture and suitable surface viscosity and mechanical strength, and meets the requirement for the film-forming property and stretchability.

The invention belongs to the field of tissue engineering scaffolds, and discloses a preparation method and application of an in-situ injection molded thiolated polysaccharide-based hydrogel and a drugcarrier thereof. The in-situ injection molded thiolated polysaccharide-based hydrogel is prepared from 3-6%(w/v) of thiolated polysaccharide and 10-29% (w/v) of sodium beta-glycerophosphate, whereinthe thiolated polysaccharide can coat one or more of drug-carrying liposomes, drug-carrying thiolated halloysite or polypeptides to form the in-situ injection molded thiolated polysaccharide-based hydrogel drug carrier. The in-situ injection-molded thiolated polysaccharide-based hydrogel and the drug carrier thereof can be gelled at human physiological temperature, and have temperature sensitivity, injectability and water absorption.

The invention relates to the technical field of serum 5'-ribotide hydrolase detection, in particular to a serum 5'-ribotide hydrolase detection reagent. A PIPES buffer solution is adopted, and various stabilizers are added to remarkably improve the stability of the reagent; beta-sodium glycerophosphate, bilirubin oxidase and ascorbic acid oxidase are added to effectively prevent interference caused by alkaline phosphatase, cholerythrin and ascorbic acid and greatly enhance the anti-interference capability of the reagent. Besides, a preferred novel amphoteric surfactant, dodecyl dimethyl betaine (BS-12), is added to prevent a reaction system from turbidity, enhance the stability of a substrate and improve the anti-interference capability of the reagent.