Preparation method and application of bioactive factor loaded temperature-sensitive composite gel carrier

A bioactive factor and composite gel technology, applied in the field of bioactive factor sustained-release carrier preparation, can solve problems such as affecting patients' chewing function, facial aesthetics, and difficulty in denture repair.

Active Publication Date: 2014-07-16
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alveolar bone atrophy not only makes it difficult to restore the denture, but also affects the patient's masticatory function and facial appearance

Method used

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  • Preparation method and application of bioactive factor loaded temperature-sensitive composite gel carrier
  • Preparation method and application of bioactive factor loaded temperature-sensitive composite gel carrier
  • Preparation method and application of bioactive factor loaded temperature-sensitive composite gel carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: Dissolve sodium alginate in 100 mL of distilled water at 60°C, mechanically stir for 10 minutes, ultrasonicate for 15 minutes, adjust the pH of the colloid to 4 with acetic acid, and make 2wt% sodium alginate hydrosol;

[0027] Step 2, take the sodium alginate hydrosol prepared in step 1 respectively, add chitosan under stirring conditions, mechanically stir at 60 ° C for 10 minutes, and ultrasonically 15 minutes, the sodium alginate / The relative weight ratio of chitosan is 0.2;

[0028] Step 3, prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add sodium alginate-chitosan hydrosol under the condition of stirring the sodium glycerophosphate aqueous solution, and control the sodium glycerophosphate / chitosan hydrosol because the pH of the sol is 4 to 5. The mass ratio is 18, forming a homogeneous sol;

[0029] Step 4, add adiponectin at 0.1% to 0.3% by weight of the homogeneous sol to the homogeneous sol prepared in step 3 unde...

Embodiment 2

[0035] Step 1: Dissolve sodium alginate in 100 mL of distilled water at 60°C, mechanically stir for 10 minutes, ultrasonicate for 15 minutes, adjust the pH of the colloid to 4 with acetic acid, and make 2wt% sodium alginate hydrosol;

[0036] Step 2, take the sodium alginate hydrosol prepared in step 1 respectively, add chitosan under stirring conditions, mechanically stir at 60 ° C for 10 minutes, and ultrasonically 15 minutes, the sodium alginate / The relative weight ratio of chitosan is 0.4;

[0037] Step 3, prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add sodium alginate-chitosan hydrosol under the condition of stirring the sodium glycerophosphate aqueous solution, and control the sodium glycerophosphate / chitosan hydrosol because the pH of the sol is 4 to 5. The mass ratio is 22.4, forming a homogeneous sol;

[0038] Step 4, add adiponectin at 0.1% to 0.3% by weight of the homogeneous sol to the homogeneous sol prepared in step 3 unde...

Embodiment 3

[0043] Step 1: Dissolve sodium alginate in 100 mL of distilled water at 60°C, mechanically stir for 10 minutes, ultrasonicate for 15 minutes, adjust the pH of the colloid to 5 with hydrochloric acid, and make 3wt% sodium alginate hydrosol;

[0044] Step 2, take the sodium alginate hydrosol prepared in step 1 respectively, add chitosan under stirring conditions, mechanically stir at 60 ° C for 10 minutes, and ultrasonically 15 minutes, the sodium alginate / The relative weight ratio of chitosan is 0.8;

[0045] Step 3, prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add sodium alginate-chitosan hydrosol under the condition of stirring the sodium glycerophosphate aqueous solution, and control the sodium glycerophosphate / chitosan hydrosol because the pH of the sol is 4 to 5. The mass ratio is 15, forming a homogeneous sol;

[0046] Step 4, add adiponectin at 0.1% to 0.3% by weight of the homogeneous sol to the homogeneous sol prepared in step ...

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Abstract

The invention relates to a preparation method and an application of a bioactive factor loaded temperature-sensitive composite gel carrier. The method comprises the following steps: 1. preparing a sodium alginate hydrosol; 2. preparing a sodium alginate-chitosan hydrosol through the hydrosol prepared by the step 1; 3. preparing a sodium glycerophosphate solution, adding the sodium alginate-chitosan hydrosol under a stirring condition to obtain a uniform sol; 4. adding adiponectin to the uniform sol prepared by the step 3, adding Ca<2+> and uniformly stirring; and 5. adding calcined bone powder to the sol prepared by the step 4 and uniformly mixing to obtain the bioactive factor loaded temperature-sensitive composite gel carrier. The preparation method, for shortcomings of the prior art of repairing missing and defects of an extraction socket wall, can greatly improve bioactivity, biocompatibility, mechanical tolerance and a slow release performance of a normal gel carrier, thus providing a novel repairing material for treatment of repairing missing and defects of the extraction socket wall.

Description

technical field [0001] The invention belongs to the technical field of preparation of bioactive factor slow-release carrier, in particular to a preparation method of a temperature-sensitive composite gel carrier loaded with bioactive factor, and the composite gel carrier is used in orthopedic repair, especially alveolar bone of tooth extraction socket Applications in wall-sparing therapy. Background technique [0002] After the tooth is extracted due to trauma, inflammation and other causes, the remaining alveolar ridge will undergo continuous bone resorption, resulting in a decrease in the height and narrowing of the remaining alveolar bone. Alveolar bone atrophy not only makes denture repair difficult, but also affects the patient's masticatory function and facial appearance. Therefore, the research on the mechanism and prevention technology of residual alveolar bone resorption after tooth loss has important clinical application value for the development of new therapeuti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/52A61L27/54
Inventor 唐志辉李箐廖运茂
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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