The invention provides a preparation method and application of
dental pulp stem cells, and belongs to the technical field of medicines. The method comprises the following steps: adding an alpha-MEM basic culture solution containing a complexing agent, a
growth promoting factor and a natural
antibacterial agent into a glass culture dish subjected to surface treatment, adding cleaned and
cut fresh human dental pulp tissues, performing constant-temperature culture, after primary cells
climb out, pouring out liquid, collecting the primary cells, re-suspending part of the primary cells in a
cell cryopreservation solution, and performing
cryopreservation, thereby obtaining the human dental pulp
tissue culture medium. And adding the remaining primary cells into a culture medium containing nano activated particles, subculturing under the conditions of
low oxygen, low-frequency
magnetic field and illumination, collecting subcultured cells, resuspending the subcultured cells in a
cell cryopreservation solution, and cryopreserving to obtain the
dental pulp stem cells. The preparation method is simple, the cost is low, industrial application is easy to achieve, the culture period is short, the
dental pulp stem cells obtained through culture are high in viability and good in differentiation performance and can be differentiated into osteoblasts, and a foundation is laid for preparing
medicine for treating tooth defects and
tooth loss.