The application provides a preparation method and application of
dental pulp stem cells, and belongs to the technical field of medicines. An alpha-MEM basic culture solution containing a complexing agent, a
growth promoting factor and a natural
antibacterial agent is added into a surface treated glass culture dish, fresh human dental pulp tissue after cleaning and
cutting is added, constant temperature culture is carried out, after primary cells are climbed out, liquid is poured out, the primary cells are collected, part of the primary cells is resuspended in a
cell freezing solution, freezing is carried out, the remaining part of the primary cells is added into a culture medium containing nano-activated particles, subculture is carried out under
low oxygen,
low frequency magnetic field and light conditions, the subculture cells are collected, resuspended in the
cell freezing solution and frozen, and the
dental pulp stem cells are prepared. The preparation method is simple, the cost is low, industrial application is easy to realize, the culture period is short, the
dental pulp stem cells obtained through culture have strong survival ability and good differentiation, can be differentiated into osteoblasts, and lay a foundation for preparing medicines for treating tooth defects and
tooth loss.