80results about How to "Avoid toxicity" patented technology

The invention relates to the field of hydrogel materials, in particular to a hydrogel dressing as well as a preparation method and application thereof. The deferoxamine mesylate is loaded on the gelatin microsphere material constructed by the gelatin and the genipin, so that the loading dispersion of the deferoxamine mesylate can be benefited, and the added gelatin can be in contact with matrix proteinase-9 in a diabetic foot ulcer microenvironment for degradation so as to induce the release of the deferoxamine mesylate. A first cross-linked network is formed by the 3-carboxyphenylboronic acid modified chitosan and the oxidized hyaluronic acid, and the gelatin microsphere material loaded with the deferoxamine mesylate and the epigallocatechin gallate are coated with the first cross-linked network. The hydrogel dressing realizes controllable release of the deferoxamine mesylate and the epigallocatechin gallate, so that the technical problem of uncontrollable release of the deferoxamine mesylate is solved.

The present disclosure relates to treatment of tumors with an anti-HER2 antibody drug conjugate in combination with an anti-HER2 antibody. Specifically, the invention relates to an application of an antibody drug conjugate combined with an antigen binding protein combined with HER2 in preparation of drugs for treating tumors, and the structure of the antibody drug conjugate is as shown in formula (I). # imgabs0 #

The invention discloses a method for constructing recombinant hansenula polymorpha for efficiently producing canine serum albumin and an expression method of the recombinant hansenula polymorpha. Based on a natural sequence of a canine serum albumin gene, a codon of the canine serum albumin gene is optimized, so that the canine serum albumin gene is more suitable for being expressed in hansenula polymorpha, an optimal promoter of the sequence expressed in hansenula polymorpha is further screened, and the copy number of the canine serum albumin in hansenula polymorpha is optimized. The yield of the canine serum albumin hansenula polymorpha strain obtained by recombinant transformation can reach 0.8-0.9 g / L after 6 days of high-density fermentation production in a fermentation tank.

The invention relates to the technical field of batteries, in particular to a hydrogel adhesive, a preparation method thereof, a negative electrode and a battery. The invention discloses a hydrogel adhesive which comprises the following raw materials: a flexible hydrogel polymer, an alginate polymer and a self-healing polymer, and the weight ratio of the flexible hydrogel polymer to the alginate polymer to the self-healing polymer is (3.2-3.5): 1: (3.2-3.5). The hydrogel adhesive provided by the invention has a chain entanglement foam structure, can effectively solve the problem of expansion of a Si-based negative electrode, and improves the cycle life and cycle stability of a battery.

The invention discloses a multifunctional bionic coating with osteogenesis induction and antibiosis functions and a preparation method thereof.The multifunctional bionic coating takes a biomedical material as a substrate and sequentially comprises a bone-imitating porous titanium dioxide nanotube layer, a phase-transition lysozyme nano-protein layer chelated with active metal ions and a bone-targeting biomolecule layer from inside to outside; the preparation method comprises the following steps: preparing a hollow bone-imitating porous titanium dioxide nanotube as a storage chamber on the surface of a biomedical material by adopting an anodic oxidation method, then preparing a phase-transition lysozyme protein layer chelated with active metal ions through in-situ self-assembly, and finally chemically connecting limb bone targeting biomolecules on the surface of the protein layer. And forming a bone targeting biomolecular layer. The multifunctional bionic coating can effectively control release of active metal ions, improve osteoblast activity and induce osteoblast adhesion and hydroxyapatite formation, and also has excellent antibacterial performance; and the preparation method is simple and easy to implement, and has a wide application prospect in the field of bone repair materials.

The invention relates to the technical field of collagen dressings, in particular to a moisturizing and repairing collagen dressing and a preparation method thereof. The moisturizing and repairing collagen dressing is prepared from the following components in parts by mass: 10 to 30 parts of recombinant human fibronectin, 10 to 20 parts of recombinant collagen, 5 to 15 parts of beta-glucan, 5 to 10 parts of compound hexanediol, 40 to 50 parts of carbomer, 5 to 15 parts of a flavoring agent, 80 to 120 parts of purified water, 10 to 30 parts of a modified recombinant human III type collagen solution and 10 to 20 parts of a modified chitosan solution. The recombinant human fibronectin disclosed by the invention can be deposited at an injured part to form a blood clot, so that the aim of stopping bleeding is fulfilled, and the subcutaneous tissue is protected; the recombinant collagen has high biological activity and can inhibit inflammation and improve the conditions of pachulosis, damage and the like of the skin; the beta-glucan can generate cytokines, promote healing of skin with laser-dependent dermatitis or barrier damage, and realize anti-allergic and anti-inflammatory effects.

The invention discloses a three-phase process for producing medium-chain fatty acid by using kitchen waste, which comprises the following steps of: (1) collecting the kitchen waste, pretreating the kitchen waste, stirring the kitchen waste by using a stirrer until the kitchen waste is pasty, and storing the pasty kitchen waste for later use; (2) taking a reaction container, adding the kitchen waste slurry and anaerobic sludge, adjusting the pH value, maintaining an anaerobic environment in the reaction container, and carrying out anaerobic fermentation to generate short-chain acid; (3) adding the kitchen waste slurry into another reaction container, adding saccharomycetes, adjusting the pH value, maintaining an anaerobic environment in the reaction container, and carrying out anaerobic fermentation to generate ethanol; and (4) centrifugally filtering the fermentation supernatant in the steps (2) and (3), taking another reaction container, mixing the filtered supernatant in the steps (2) and (3), inoculating the domesticated chain extension sludge, adjusting the pH value, maintaining the anaerobic environment in the reaction container, and carrying out chain extension reaction to generate the medium-chain fatty acid. The process disclosed by the invention realizes multi-dimensional value-added utilization of producing short-chain acid, ethanol and medium-chain fatty acid from the kitchen waste.

The invention discloses a preparation method and application of a porous GelMA microneedle for delivering AIE molecules based on HSF derived exosome. The preparation method comprises the following steps: S1, preparing TTMN molecules; s2, dispersing DPPC and Chol in chloroform, performing water bath evaporation at 50 DEG C to form a thin film, hydrating the thin film in PBS, incubating to enable DPPC and Chol to form a liposome structure again, and uniformly dispersing TTMN molecules in the liposome structure to obtain TL; s3, extracting an exosome from the HSF cells; s4, loading the TL into the exosome by utilizing an electroporation technology, so as to obtain TE; s5, preparing the GelMA; and S6, mixing the GelMA with the TE, so that the TE is uniformly dispersed in the GelMA to obtain a mixed solution, injecting the mixed solution into a microneedle mold, and irradiating with 405nm laser to obtain the porous GelMA microneedle. According to the invention, high-efficiency treatment of hypertrophic scars is realized, and the system has a remarkable drug delivery effect, an innovative photodynamic therapy, a high-efficiency scar tissue remodeling effect and good biological safety and stability, and has important clinical application value and market prospect.

The invention discloses a bisphenol AF feeding dust prevention device, which relates to the technical field of feeding dust prevention, and comprises a conveying device, wherein both ends of the conveying device are fixedly mounted with load-bearing plates, a rotating motor is fixedly mounted on the surface of the conveying device, a supporting foot is fixedly mounted on the bottom of the load-bearing plate, vertical rods are fixedly mounted on the top of the load-bearing plate, a feeding frame is fixedly mounted between the vertical rods, a feeding port is provided at the bottom of the feeding frame, a top cover is fixedly mounted on the top of the feeding frame, a feeding port is provided on the surface of the top cover, a partition plate is fixedly mounted inside the feeding frame, a No. 1 spring is fixedly mounted on the top of the partition plate, and the partition plate slowly flows out through the opening of a material distribution funnel, and bisphenol AF does not generate dust when feeding, so that the dust generated by bisphenol AF during rapid feeding is prevented from being flammable or toxic with certain chemical dust, thereby reducing the risk of explosion and poisoning.

A cooling device for an explosion-proof electric control box and a method for using the same, the cooling device comprising: a shell, a coolant tank, a coolant spray device, a controller, a spray electromagnetic switch and a plurality of temperature sensors; the coolant spray device is provided with a compressed air pipe, a liquid inlet pipe and a spray manifold, the compressed air pipe is connected to the compressed air source pipeline, the liquid inlet pipe is connected to the inside of the coolant tank, the spray manifold is provided with a plurality of spray ports, and a spray electromagnetic switch is provided on the opening of each spray port, the temperature signal input end of the controller is respectively connected to each temperature sensor, and a group of spray signal output ends of the controller are respectively connected to the spray signal input ends of each spray electromagnetic switch. This design can not only cool down different areas in the shell through the cooperation of the temperature sensor, the controller and the spray electromagnetic switch, effectively improving the heat dissipation efficiency of the electric control box, but also the cooling device will not generate discharge sparks, effectively improving the safety of the system.

The invention belongs to the technical field of polyurethane coatings, and particularly discloses a weather-resistant polyurethane waterproof coating and a preparation method thereof. The polyurethane waterproof coating comprises a component A and a component B, wherein the component A is prepared from the following raw materials: diphenylmethane diisocyanate and polyether glycol; and the component B is prepared from the following raw materials: polyether triol, water, a catalyst, a nano metal oxide, a coupling agent and a pigment filler. The polyurethane waterproof coating is safe and environment-friendly, the cured waterproof coating has excellent weather resistance and mechanical property, the preparation process is simple, the construction stability is high, the polyurethane waterproof coating is suitable for waterproof engineering in constructional engineering, the engineering quality can be improved, and the later maintenance cost can be reduced. The invention also provides a preparation method and application of the polyurethane waterproof coating.

The present invention relates to the field of medical technology, and provides a PMC nanozyme and a preparation method thereof; the PMC nanozyme comprises: a core layer, the core layer comprises oxygen atoms, phosphate ions and polyoxometalate nanoclusters of a first transition metal element; a coating layer, at least part of the coating layer is fixed on the outer surface of the core layer, and the coating layer is formed by the coordination between a second transition metal and the oxygen atoms on the core layer; wherein the first transition metal element comprises Mo; the second transition metal element comprises Mn, and the coating layer comprises a CO donor for controlling the release of CO gas. The PMC nanozyme of the present invention can utilize polyoxometalate nanoclusters in combination with CO gas therapy to synergistically treat IBD, and on the basis of endowing the nanoclusters with anti-inflammatory and intestinal epithelial barrier repair functions, the catalytic activity is enhanced, and CO is released in an environment of overexpression of reactive oxygen species, thereby alleviating oxidative stress, inhibiting inflammatory responses, restoring intestinal immune balance, and promoting the repair of mucosal damage.

The invention discloses a selenium-rich dendrobium polysaccharide and a preparation method thereof, wherein the preparation method comprises the following steps: 1) taking a dendrobium officinale extract and adding a nitric acid solution to dissolve it; 2) taking the solution obtained in step 1), adding sodium selenite and barium chloride to react for 6 to 10 hours, cooling and filtering, taking the filtrate to adjust the pH value to 4 to 7, dialyzing, taking the dialyzate and drying, and obtaining the selenium-rich dendrobium polysaccharide. The preparation method of the selenium-rich dendrobium polysaccharide of the invention, through specific synthesis process parameters and a concentration and purification method, the total polysaccharide content in the selenium-rich dendrobium polysaccharide is greater than 90%, and the selenium content is greater than 1.2 mg / g. Experiments have proved that it has significant antioxidant and anti-wrinkle activities.

This invention relates to anti-CD70 antibodies and antibody drug conjugates comprising at least one non-naturally encoded amino acid. Disclosed herein are αCD70 antibodies with one or more non-naturally encoded amino acids and further disclosed are antibody drug conjugates wherein the αCD70 antibodies of the invention are conjugated to one or more toxins. Further disclosed are methods for using such non-natural amino acid antibody drug conjugates, including therapeutic, diagnostic, and other biotechnology uses.

The invention provides an X-ray composite radiation shielding material and a preparation method and application thereof, and belongs to the technical field of radiation protection. The photoelectric effect and the scattering effect of X-rays in the material are remarkably enhanced by using high atomic number elements contained in the metal oxide, so that the transmittance of the X-rays is effectively reduced. Meanwhile, the oxide material has good chemical stability and environmental friendliness, and the toxicity and aging problems caused by a traditional lead-based shielding material can be avoided. The prepared composite radiation shielding material has the advantages of being low in cost, light in weight, easy to process, good in flexibility, non-toxic, environmentally friendly and the like, the shielding efficiency of an X-ray shielding coating reaches up to 70% or above when the X-ray photon energy is 70 kV or below, and the generated secondary reflectance can be reduced by 35-80% compared with lead.

The invention belongs to the field of biomedical engineering, and particularly relates to high-water-solubility fullerol and application of the high-water-solubility fullerol in treatment of intervertebral disc degeneration. The chemical formula of the high-water-solubility fullerol is as shown in formula 1. Compared with the prior art, the preparation method at least has the following beneficial effects: (1) a novel fullerol is synthesized, and a branched chain of the fullerol contains a plurality of hydroxyl groups, so that the water solubility of the fullerol is enhanced, and cytotoxicity caused by aggregation due to poor water solubility is avoided; (2) the use mode and the use dosage of the fullerol in a rat IDD model are defined, and a foundation is laid for subsequent clinical transformation of the fullerol. And (3) the action target of the fullerol is determined to be Rap1, and downstream Nrf2 protein can be activated, so that the result proves that the fullerol is a potential Rap1 and Nrf2 agonist, which is not determined in the past.

This invention relates to the field of artificial bear bile powder technology, specifically to a highly efficient cloning and temperature-inducible expression vector for the 7αβ-hydroxysteroid dehydrogenase gene and its construction method. The expression vector is formed by integrating the hydroxysteroid dehydrogenase gene into the pBV220 plasmid; the hydroxysteroid dehydrogenase gene is either the 7α-hydroxysteroid dehydrogenase gene or the 7β-hydroxysteroid dehydrogenase gene. The construction of this expression vector makes it possible to obtain the protease through induced fermentation using a temperature-inducible expression system, avoiding the introduction of toxic inducers and simplifying the process. During vector construction, the target gene and the linear plasmid ligate spontaneously within the engineered bacteria, independent of endonucleases and ligases, further reducing costs and process complexity. This technical solution can solve the technical problem of toxic residues in the engineered bacterial expression products of hydroxysteroid dehydrogenase in existing technologies, and has ideal prospects for widespread application.

This invention belongs to the field of new energy and hydrogen energy, and specifically relates to an integrated energy vessel, the "Dongfang Heping," for direct seawater hydrogen production, storage, and conversion. The integrated energy vessel of this invention includes a powered platform, on which are installed a wave energy system, a photovoltaic system, a wind power system, a seawater desalination direct electrolysis hydrogen production system, a green methanol synthesis system, a green ammonia synthesis system, and a power system. The wave energy system, photovoltaic system, and wind power system are used to extract energy from the ocean and convert it into electrical energy to provide power to the powered platform, the seawater desalination direct electrolysis hydrogen production system, the green methanol synthesis system, and the green ammonia synthesis system. The power system drives the powered platform, and the seawater desalination direct electrolysis hydrogen production system, the green methanol synthesis system, and the green ammonia synthesis system are electrically connected to the power system. This invention provides a comprehensive energy ship system that integrates multiple renewable energy sources such as photovoltaic, wind power, and wave energy. It constructs a power platform at sea that integrates the production, storage, transportation, and utilization of "oceanic green hydrogen," forming an integrated energy production and conversion model of "green electricity-green hydrogen-green ammonia and alcohol." This not only meets the shipping industry's demand for green fuels but also effectively solves the problem of renewable energy consumption, promoting the deep integration of transportation and energy.

The invention provides an efficient near-infrared light-emitting zero-dimensional tin-based perovskite material as well as a preparation method and application thereof. The chemical formula of the zero-dimensional tin-based perovskite material is (C8NH18) 2SnX4, X is selected from ClxBr1-x or Br, and x is selected from 0 to 1 and is not 0. The invention effectively solves the problems of toxicity of the known lead-containing perovskite material and low luminous efficiency of the near-infrared perovskite material, is expected to realize the application of the perovskite material in the fields of human electronic products and the like, and has huge development potential in the fields of luminous display, optoelectronic devices and the like.

The invention discloses a tartary buckwheat protein compound as well as a preparation method and application thereof, and relates to the technical field of preparation of Pickering emulsion stabilizers. The method comprises the following steps: diluting tartary buckwheat protein hydrogel with distilled water, and adjusting the pH value to prepare protein hydrogel; mixing quercetin with an ethanol solution, and adjusting the pH value to prepare a quercetin solution; and mixing the protein hydrogel with a quercetin solution, heating in a water bath, carrying out ultrasonic emulsification, and freeze-drying to obtain the tartary buckwheat protein compound. The invention provides a non-toxic, harmless and pollution-free natural emulsifier and stabilizer, simultaneously prepares a Pickering emulsion capable of loading functional substances and nutritional ingredients, and provides reference for screening research of the natural emulsifier, basic research of tartary buckwheat protein, development of tartary buckwheat products and protection of nutritional ingredients easy to photodecompose and oxidize. The problem that in the prior art, the effect of protein serving as a Pickering emulsion stabilizer is poor is solved.

The invention belongs to the technical field of hydrogel, particularly relates to A61K9 / 06, and more particularly relates to bi-component polyelectrolyte compound drug-loaded gel and a preparation method thereof. The bi-component polyelectrolyte compound drug-loaded gel is prepared from the following raw materials: a drug component, positively charged high-molecular polymer polyethyleneimine and negatively charged high-molecular polymer polyacrylic acid. The bi-component polyelectrolyte compound drug-loaded gel provided by the invention can deliver various drugs, including dexamethasone, and the drug-loaded gel can be applied to allergic inflammation and autoimmune inflammatory diseases, and also can be applied to local skin diseases and the like; the product can relieve acute inflammation in a short time, and can also realize a long-term slow release effect.

The invention relates to a mitochondrial delivery system and a preparation method and application thereof. According to the mitochondrial delivery system, safe and efficient cell entry of active mitochondria can be achieved, the mitochondrial delivery system has appropriate particle size and surface potential, toxicity to cells is avoided while the entrapment rate of the mitochondria is effectively improved and the electrochemical stability and biological activity of a mitochondrial membrane are guaranteed, and the mitochondrial cell entry efficiency is remarkably improved. The mitochondrial delivery system not only can transplant active mitochondria into common cells to repair cell damage, but also can transplant the active mitochondria into egg cells to play a role across a zona pellucida and a plasma membrane barrier. The mitochondrial delivery system can be used for treating mitochondrial diseases related to mitochondrial dysfunction, especially infertility caused by mitochondrial dysfunction of egg cells.

The invention discloses an osteogenesis-promoting and anti-infection difunctional porous metal antibacterial coating material based on a bimetal ion electroplating and electrolytic pore-forming technology and a preparation method of the porous metal antibacterial coating material. The preparation method comprises the following steps: 1) ultrasonically cleaning a titanium substrate by using an environment-friendly degreasing agent and deionized water to remove impurities and chemical films on the surface of titanium; 2) forming a copper transition layer on the surface of the substrate by using a chemical deposition process; 3) designing a Cu < 2 + > / Mg < 2 + > composite electrolyte formula, and further introducing a copper-magnesium composite coating on the surface of the transition layer in combination with a bimetal ion electroplating process; (4) an alkaline stripping solution is designed for electrolytic stripping of magnesium, and a porous copper coating is obtained; and (5) a sample is soaked in a blood vessel promoting medicine, and after ultrasonic soaking, low-temperature nitrogen drying, dark vacuum packaging and irradiation sterilization are conducted, the required porous copper coating material with the osteogenesis promoting and anti-infection functions is obtained. The coating material can induce cell adhesion and ingrowth at the same time, synergistically promote bone formation, and opens up a brand new strategy for bone repair.

The invention relates to a stem cell preparation for treating Parkinson's disease and a preparation method thereof, and belongs to the technical field of biological medicines.The preparation method comprises the following steps that a traditional Chinese medicine extract is encapsulated through solid lipid nanoparticles, ultrasonic treatment is conducted, and a compound substance is obtained; coating the compound with quaternized chitosan and hyaluronic acid, so as to obtain an encapsulated compound; and dispersing neural stem cells in a phosphate buffer solution to obtain a cell suspension, dispersing the encapsulated compound in deionized water to obtain a dispersion liquid, and adding the dispersion liquid into the cell suspension to obtain the stem cell preparation for treating Parkinson's disease. According to the technical scheme, the traditional Chinese medicine extract is doubly encapsulated by the solid lipid nanoparticles, the quaternized chitosan and the hyaluronic acid, so that active ingredients in the traditional Chinese medicine extract can be effectively protected, the biological activity and the stability of the traditional Chinese medicine extract are improved, proliferation and differentiation of stem cells are promoted, and the survival rate of the stem cells is increased. And the stem cell preparation can have a better effect in the aspect of preventing and treating Parkinson's disease.

The invention discloses a patulin nano antibody and application thereof, patulin artificial antigen (conjugate of patulin and carrier protein BSA) is used as coating antigen, a nano antibody natural library is used for screening the patulin nano antibody, and the patulin artificial antigen does not need to be used for immunizing camelidae animals. The toxic effect of patulin on animals is avoided; according to the present invention, the patulin nano-antibody is prepared by using the patulin nano-antibody, the patulin immunodetection method is established by using the patulin nano-antibody, the patulin detection limit of the patulin nano-antibody is 0.12 [mu] g / mL, the half inhibition concentration is 0.41 [mu] g / mL, and the linear range is 0.17-0.97 [mu] g / mL, and the method has characteristics of simpleness, rapidness, strong specificity and high sensitivity, and can be used for rapid detection of patulin in food.

The application discloses a preparation method of a multifunctional nano drug of Fe-based MOF loaded CM-272, and relates to a preparation method of an anti-cancer nano drug.The application aims at solving the problem that the CM-272 action is limited due to the internal hypoxic environment of a tumor.Method: I, preparing a Fe-based metal organic framework;II, preparing a MIL-53@CM-272 nano drug.The application is used for the preparation of the multifunctional nano drug of the Fe-based MOF loaded CM-272.

An object to be solved of the present invention is to provide a brain-penetrable antitumor agent showing an excellent brain penetration property and RET inhibitory activity. The present invention provides brain-penetrable antitumor agent including a compound represented by Formula (I) below or a salt thereof as an active ingredient: wherein R1, R2, and R3 are as described in Specification.

The invention relates to the technical field of gene engineering, and particularly provides a regulation and control system for degrading uric acid to reduce the uric acid level and application thereof.The system comprises a first functional gene, a second functional gene and a third functional gene, and the first functional gene encodes a first functional protein capable of degrading the uric acid; the first functional protein comprises one or more sequences as shown in SEQ ID NO: 1-10 or having at least 85% identity with the first functional protein; a second functional gene encoding a second functional protein capable of transporting uric acid, the second functional protein comprising one or more sequences as shown in SEQ ID NO: 11-15 or having at least 85% identity therewith; the first promoter is used for regulating and controlling the expression of the first functional gene and / or the second functional gene; the first terminator is used for terminating the expression of the first functional gene and / or the second functional gene. The regulation and control system for degrading the uric acid to reduce the uric acid level, provided by the embodiment of the invention, shows an efficient uric acid degradation function.

The present invention provides an online selective separation device for organic acid from anaerobic digestion liquid, which is connected to an anaerobic reactor in which digestion liquid is placed, and comprises: a box body, a cathode and an anode arranged at both ends of the box body, and multiple groups of bipolar membranes and anion exchange membranes alternately stacked between the cathode and the anode, wherein the multiple groups of bipolar membranes and anion exchange membranes divide the box body into multiple compartments, namely, a cathode chamber, an anode chamber, and multiple alkali chambers and multiple acid chambers arranged between the cathode chamber and the anode chamber, the alkali chamber and the anaerobic reactor are connected by a pipeline to form a loop, and a microfilter for microfiltering the digestion liquid is arranged on the pipeline of the digestion liquid in the anaerobic reactor entering the alkali chamber, and a membrane contactor is embedded in the acid chamber. The present invention greatly saves energy consumption and improves the stability and efficiency of anaerobic digestion.

The present invention discloses a gold homodimer (AuHDs) probe based on anisotropic scattering properties and uniform surface chemical properties, a preparation method and an application thereof. The present invention also discloses a preparation method of the AuHDs probe obtained by a stepwise growth method and DNA modification and purification, wherein the synthesized probe has uniform physical and chemical properties and dumbbell-shaped anisotropic scattering properties. The present invention also discloses a single particle tracking (SPT) method assisted by a colorimetric algorithm (CBA), which can be used to accurately track the rotational motion of the AuHDs probe under a dark field microscope (DFM). The present invention brings new inspiration to the development of anisotropic imaging probes in various cellular processes and the dynamics study of vesicle movement during endocytosis, and has broad application prospects.