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37 results about "Arthrobacter simplex" patented technology
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Biological dehydrogenation preparation method of steroid drug intermediate
InactiveCN101760496AAvoid residueAvoid toxicityMicroorganism based processesFermentationArthrobacter simplexSolvent
A biological dehydrogenation preparation method of steroid drug intermediate takes the formula (1) compound as a substrate, and adopts an arthrobacter simplex biological dehydrogenation method to obtain formula (2) compound; the special process comprises the following steps: smashing or dissolving the formula (1) compound with solvent, putting into a fermentation tank in which arthrobacter is cultivated for biotransformation, extracting, separating and refining, and drying to obtain dehydrogenated polymer, i.e. compound (2); and the dehydrogenation transformation rate can reach 70 to 90 percent.
Owner:TIANJIN JINYAO GRP
Method for producing prednisolone acetate
InactiveCN101210259AStrong response specificityEasy to operateMicroorganism based processesFermentationMicrobial transformationArthrobacter simplex
The invention belongs to the field of microbial pharmaceutics and pharmaceutical engineering, specifically relates to a production method of prednisone acetate by microbial transformation with Arthrobacter simplex as bacteria strain and hydrocortisone acetate as substrate. The method uses Arthrobacter simplex as bacteria strain and comprises the following steps of: performing primary seed culture, performing second fermentation culture, adding hydrocortisone acetate into the fermentation liquid of Arthrobacter simplex to transform hydrocortisone acetate into prednisone acetate, filtering, and collecting cake to obtain prednisone acetate. The bacteria can be prepared into double liquid phase, broken cells or protoplast, each of which has high transformation ratio. The inventive production method replaces cortisone acetate with hydrocortisone acetate as raw material, and has the advantages of high yield, simple process, good economical and practical performance, and less use of harmful reagents; and is important for steroids production with biotransformation method.
Owner:TIANJIN UNIV OF SCI & TECH
Method for preparing 11 alpha-OH-ADD by mixed fermentation of microbes
ActiveCN102827913AHigh yieldFully transformedMicroorganism based processesFermentationMicroorganismArthrobacter simplex
The invention relates to a production method of steroids medicines, and concretely relates to a method for conversing 4-AD to produce 11 alpha-OH-ADD by mixed fermentation of two microbes. According to the invention, 4-AD is taken as a raw material, and the mixed fermentation is carried out through aspergillus ochraceus ATCC18500 and Arthrobacter simplex ATCC6946. Compared with hydroxylation and dehydrogenation reaction by individually using the two above bacteria, 11 alpha-OH-ADD can be obtained through mixed fermentation of 4-AD, two above bacteria have cooperative effect on the fermentation and conversion, The method has the following advantages: the overall yield is high, the conversion is more complete, a step of post-treatment is reduced, and the energy consumption is reduced. The mixed fermentation is capable of greatly reducing the production cost, increasing the conversion rate, reducing the conversion time, and relatively reducing the post-treatment and the energy consumption.
Owner:HUNAN LONGTENG BIOTECH
Salt-resistant ammonia-nitrogen-removing compound bacterial agent for printing and dyeing wastewater treatment
PendingCN110157632AImprove biological activityHigh bacterial contentBacteriaWater contaminantsEnterobacter cloacaePseudomonas putida
The invention discloses a salt-resistant ammonia-nitrogen-removing compound bacterial agent for printing and dyeing wastewater treatment. The salt-resistant ammonia-nitrogen-removing compound bacterial agent is prepared from, by mass, 5-8% of a saline-alkaline-resistant aliidiomarina haloalkalitolerans culture, 5-7% of a salt-resistant nesterenkonia halotolerans culture, 20-22% of a zobellella taiwanensis culture, 15-18% of a comamonas aquatica culture, 10-12% of a pseudomonas putida culture, 10-15% of a bacillus subtilis culture, 5-7% of an arthrobacter simplex culture and 17-18% of an enterobacter cloacae culture. The salt-resistant ammonia-nitrogen-removing compound bacterial agent for the printing and dyeing wastewater treatment contains various strains, is reasonable in compatibility,has a strong synergistic effect, can effectively remove ammonia nitrogen and industrial COD in printing and dyeing wastewater, has low input cost, and can be promoted and used on a large scale.
Owner:新疆水处理工程技术研究中心有限公司
Method for preparing prednisone acetate
InactiveCN108588163AImprove conversion rateImprove refining efficiencyMicroorganism based processesFermentationArthrobacter simplexSolvent
Owner:ZHEJIANG XIANJU PHARMA
Method for preparing dexamethasone intermediate product through one-step microbial fermentation and transformation
ActiveCN106520896ACompletely transformedHigh yieldMicroorganism based processesFermentationDexamethasoneHydrolysate
The invention belongs to the technical field of bio-pharmaceuticals, and in particular relates to a method for producing a dexamethasone epoxide hydrolysate through one-step microbial fermentation and transformation. According to the method, with 16a-methyl-17[alpha],21-dihydroxypregna-4-ene-9,11-epoxy-3,20-dione-21 acetate as a substrate, the dexamethasone epoxide hydrolysate is directly produced mainly by conducting mixed fermentation by virtue of arthrobacter simplex and bacillus megatherium. In comparison with the prior art, the method provided by the invention, on the basis of a synergistic effect between mixed strains, integrally combines two steps of reactions, namely dehydrogenation and hydrolysis, so that the finished dexamethasone epoxide hydrolysate is directly obtained; and the product has the advantages of being short in production cycle, high in bio-transformation rate, high in yield, environment-friendly and low in cost.
Owner:JIANGSU YUANDA XIANLE PHARMA
Preparation process of betamethasone intermediate
InactiveCN102071240AHigh yieldQuality improvementMicroorganism based processesFermentationCompound (substance)Betamethasone
The invention discloses a preparation process of a compound shown in a formula II. The compound is prepared from a compound shown in a formula I by biological fermentation and dehydrogenation, and in the processes of biological fermentation and dehydrogenation, an inert grinding material is added in fermentation liquid. In the invention, the fermentation manner is improved under a condition of using a common arthrobacter simplex strain; and a proper amount of inert grinding material is added in the fermentation liquid so that the yield and the quality of a compound product (namely a betamethasone intermediate) in the formula II are improved under the condition that the feeding concentration is maintained to be over 0.5%, and the production cost of betamethasone is reduced. In addition, no chemical solvent is used in the preparation process, and thus the cost for auxiliary materials is saved.
Owner:ZHEJIANG XIANJU PHARMA
Construction method and application of Arthrobacter simplex engineering strain high in organic solvent tolerance
The invention relates to a construction method and application of an Arthrobacter simplex engineering strain high in organic solvent tolerance. The Arthrobacter simplex engineering strain is obtained by guiding a gene segment into Arthrobacter simplex; experiments verify that tolerance of the engineering strain obtained by utilizing the construction method to methanol and alcohol is improved obviously. By utilizing the engineering strain for steroid C1, 2 dehydrogenation reaction, adding amount of an organic solvent in a system is doubled, feeding concentration of a substrate is increased by three times, and generation amounts of products are increased in different degrees. By constructing and applying the converted engineering strain high in organic solvent tolerance, the adding amount of the organic solvent in the conversion system can be increased, the feeding concentration of the substrate is increased, the generation amount of the products is increased, and the engineering strain has important application value in increasing the conversion efficiency of high-concentration substrates of steroid hydrophobic compounds.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Constructing method and application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes
InactiveCN103205450AReduce manufacturing costHigh copy numberBacteriaMicroorganism based processesArthrobacter simplexMolecular Technique
The invention relates to application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes to constructing high-efficiency expression steroid compound A ring 1, 2 dehydrogenation arthrobacter simplex engineering bacteria. The plasmid is capable of being copied in arthrobacter simplex and efficiently expressing steroid compound A ring 1, 2 dehydrogenase genes and significantly practical, and basis is provided for further using plasmid pXJM19 to modify arthrobacter simplex through molecular techniques.
Owner:TIANJIN UNIV OF SCI & TECH
Mutant strain and engineering bacterium of arthrobacter simplex with stress tolerence
ActiveCN107988092AStrong toleranceImprove toleranceBacteriaHydrolasesHigh concentrationEndurance capacity
The invention belongs to the field of genetic breeding, and particularly relates to a mutant strain of arthrobacter simplex with stress tolerence and a genetically engineered bacterium constructed bythe mutant strain. The survival rate of the mutant strain under the impact conditions of 16% ethyl alcohol and 20% methyl alcohol is increased by 3.74 times and 2.10 times respectively compared with the original strain; the endurance capacity of engineering bacteria, overexpressing the genes of groEl, dnaK, recA, uvrD, katG, sod or treS from the mutant strain, to high-concentration organic solvents is obviously improved, and the endurance capacity to salt pressure and oxidative pressure is expresses to be higher. When the engineering bacteria are applied to steride C1,2 dehydrogenation reaction, the ethanol concentration in a system can be increased to 8 to 10%, the substrate CA concentration can be increased to 6 to 8g / L, the product yield can be improved by 0.41 to 3.56 times, and a goodbasis is established for the subsequent tolerance mechanism research and bacterial strain molecular modification and application.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Process for preparing 6 alpha-methyl hydroprednisone
InactiveCN1966711AHigh purityRaw materials are easy to getMicroorganism based processesFermentationDiketoneVitamin K3
The invention provides a method for preparing 6alpha-medrol (1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane), and is characterized in the following method: adopting 4-alkene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane as the substrate, fermentation enzyme solution obtained from the fermentation of Arthrobacter simplex as enzyme source, adding 0.01-0.08g / 100mL fermentation enzyme solution vitamin K3, carrying out a converting reaction at 25-35DEG C for 36-60h till complete reaction, separating and purifying to obtain the final product1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane. The beneficial effects of the biological preparation method of 1,4-diene-6alpha-methyl-3,20-diketone-11 beta,17alpha-dihydroxyl pregnane is mainly reflected in: (1) wide raw materials sources, and low cost, (2) good selectivity, simple process, industrial production, (3) mild reaction conditions, easy separation, highly pure product.
Owner:ZHEJIANG UNIV OF TECH
Method for preparing prednisolone through bio-fermentation in one step
The invention relates to a method for directly generating prednisolone through bio-fermentation in one step by using hydrocortisone acetate as a raw material and Arthrobacter Simple ATCC 21032.
Owner:TIANJIN JINYAO GRP
Production method of methylprednisolone dehydrogenate
ActiveCN112608970ACause toxic effectsQuality impactMicroorganism based processesFermentationBiotechnologyMicroorganism
The invention provides a production method of a methylprednisolone dehydrogenate. The production method of the methylprednisolone dehydrogenate comprises the following steps: A) adding water into a methylprednisolone Grignard substance, and pulping to obtain slurry; and B) biologically converting the methylprednisolone Grignard substance in the slurry into a methylprednisolone dehydrogenized substance by using arthrobacter simplex. According to the production method, no organic solvent is used, and the toxic effect on microorganisms is small during biotransformation; according to the method, the methylprednisolone Grignard substance is added with water and pulped into slurry, so that the feeding concentration and the substrate conversion rate of the methylprednisolone Grignard substance are greatly improved, the yield of a methylprednisolone dehydrogenized substance product is high, and the method is particularly beneficial to industrial production of the methylprednisolone dehydrogenized substance.
Owner:HENAN LIHUA PHARMA
Preparation of boldenone by sequential conversion of Arthrobacter simplex and genetically-engineered yeast strain
ActiveCN109971817AHigh yieldImprove coenzyme utilizationMicroorganism based processesOxidoreductasesMicroorganismYeast
The invention relates to a production method of steroidal drugs, in particular to a method of preparing a boldenone steroidal compound by sequential fermentation of Arthrobacter simplex and a genetically-engineered yeast strain. The method comprises: providing a Pichia pastoris genetically-engineered strain capable of efficiently expressing 17beta carbonyl reductase; providing a process of fermenting the compound AD by microbial sequential conversion to prepare a compound BD, wherein the method refers to fermenting the AD via Arthrobacter simplex, deactivating the reaction system, and preparing the compound boldenone through sequential fermentation of the Pichia pastoris genetically-engineered strain.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for preparing 16alpha, 17alpha-epoxy-11alpha-hydroxy-pregna-1,4-diene-3,20-dione through combined microbial fermentation
InactiveCN105779556AHigh yieldReduce stepsMicroorganism based processesFermentationKetoneArthrobacter simplex
The invention discloses a method for preparing 16alpha, 17alpha-epoxy-11alpha-hydroxy-pregna-1,4-diene-3,20-dione by taking 16alpha,17alpha-epoxy progesterone as a starting material through one-step mixed fermentation of aspergillus ochraceus and arthrobacter simplex.
Owner:TIANJIN JINYAO GRP
Method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium
ActiveCN105779553AHigh yieldReduce stepsMicroorganism based processesFermentationMicroorganismArthrobacter simplex
Owner:TIANJIN JINYAO GRP
A kind of preparation method of dexamethasone intermediate
ActiveCN110157764BSimple production processEasy to operateBacteriaMicroorganism based processesEpoxyArthrobacter
The invention discloses a method for preparing a dexamethasone epoxy hydrolysate 8DM from arthrobacter simple CPCC140451 through one-step fermentation. The method is simple in operation manner, stablein process and suitable for industrial mass production.
Owner:ZHEJIANG XIANJU PHARMA
Preparation method of prednisone acetate
InactiveCN112608969AThe product quality is quiteHigh yieldMicroorganism based processesFermentationBiotechnologyOrganic solvent
The invention discloses a preparation method of prednisone acetate, and the method comprises the following steps: adding a hydrophilic organic solvent into Arthrobacter simplex fermentation broth, and adding 8-12% of prednisone acetate micropowder; finally, adding a lipophilic organic solvent for fermentation reaction; and after the fermentation reaction is finished, filtering or centrifuging the product, performing separating, decolorizing and crystallizing to obtain prednisone acetate. The quality of the product prepared by the preparation method is equivalent to that of the product in the prior art, but the yield is slightly higher, the single-batch yield is higher, the production efficiency is higher, and the preparation method is more suitable for large-scale industrial production.
Owner:HUAZHONG PHARMA
Method for preparing 11alpha, 17alpha-dyhydroxyl-pregn-1,4-diene-3,20-dione through microbial co-fermentation
InactiveCN105779554AHigh yieldReduce stepsMicroorganism based processesFermentationKetoneArthrobacter simplex
The invention relates to a method for preparing 11alpha, 17alpha-dyhydroxyl-pregn-1,4-diene-3,20-dione by taking 17alpha-hydroxyprogesterone as a starting material and through mixed one-step fermentation adopting aspergillus ochraceus and arthrobacter simplex.
Owner:TIANJIN JINYAO GRP
Method for preparing delta 1-11 alpha,17 alpha-dihydroxyprogesterone with one-pot continuous fermentation process
InactiveCN104711311AReduce filterReduce extractionMicroorganism based processesFermentationContinuous fermentationArthrobacter simplex
The invention discloses a method for preparing delta 1-11 alpha,17 alpha-dihydroxyprogesterone with a one-pot continuous fermentation process, and belongs to the technical field of intermediate preparation. The method comprises the following specific steps: (1) inoculating slant culture of arthrobacter simplex into a first culture medium, performing culture for 20-30 hours, adding ground 17 alpha-hydroxyprogesterone, and performing transformation; (2) inoculating fermentation liquor after finishing the step (1) into a second culture medium, inoculating slant culture of aspergillus ochraceus into the second culture medium, performing culture, and performing post-treatment to obtain delta 1-11 alpha,17 alpha-dihydroxyprogesterone. Delta 1-11 alpha,17 alpha-dihydroxyprogesterone is prepared with the one-pot continuous fermentation process, so that a step of filtering, extracting, refining and drying a dehydrogenation material is reduced, discharge of 50% pollutants is reduced, environmental pressure is reduced, the time is saved, the yield is increased, the efficiency is improved, the process is simplified, the whole reaction operation is convenient, and the method is suitable for industrialized popularization and application.
Owner:JIANGXI JUNYE BIOLOGICAL PHARM CO LTD
Method for preparing 16,17α-epoxy progesterone by biological method
ActiveCN106636287BMild reaction conditionsRealize green manufacturingMicroorganism based processesFermentationEpoxyArthrobacter simplex
The invention discloses a method for preparing 16,17[alpha]-epoxyprogesterone by virtue of a biological process. By conducting biological fermentation on a 3-hydro-5-ene-16,17[alpha]-epoxy substance by virtue of autologous cholesterol oxidase of Arthrobacter simplex AS1.94, so that biological manufacturing of the 16,17[alpha]-epoxyprogesterone is achieved; therefore, a conventional chemical process is replaced and the conversion rate and yield of the 16,17[alpha]-epoxyprogesterone are improved, wherein the 3-hydro-5-ene-16,17[alpha]-epoxy substance serves as a substrate and the Arthrobacter simplex AS1.94 is a catalysis strain; and by conducting the fermentation, the yield of the 16,17[alpha]-epoxyprogesterone is greater than 90%, and the purity of the 16,17[alpha]-epoxyprogesterone is greater than 98.5%.
Owner:山东宝利甾体生物科技有限公司
Construction method and application of plasmid capable of replicating and expressing dehydrogenase gene at positions 1 and 2 of steroid compound a ring in Arthrobacter simplex
InactiveCN103205450BReduce manufacturing costHigh copy numberBacteriaMicroorganism based processesCompound aMolecular Technique
The invention relates to application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes to constructing high-efficiency expression steroid compound A ring 1, 2 dehydrogenation arthrobacter simplex engineering bacteria. The plasmid is capable of being copied in arthrobacter simplex and efficiently expressing steroid compound A ring 1, 2 dehydrogenase genes and significantly practical, and basis is provided for further using plasmid pXJM19 to modify arthrobacter simplex through molecular techniques.
Owner:TIANJIN UNIV OF SCI & TECH
Preparation method of triketone dehydrogenation product
PendingCN114196722ALow yieldImprove conversion rateBacteriaMicroorganism based processesStrong acidsEngineering
The invention relates to the technical field of pharmacy, in particular to a preparation method of a triketone dehydrogenation product, which comprises the following steps: mixing, stirring and pulping a triketone product and water to obtain slurry; adding a dispersing agent into the slurry, and sterilizing at high temperature to prepare triketone slurry; wherein the mass ratio of the triketone substance to the water is 1: (2-3); the mass ratio of the slurry to the dispersing agent is 1: (0.01-0.05); putting the triketone slurry into a fermentation culture solution of arthrobacter simplex for biotransformation in batches to obtain a triketone dehydrogenation product; wherein the total adding concentration of the triketone slurry is 2-4% of that of the fermentation culture solution. The biotransformation method can specifically remove 1, 2 hydrogen of 17 alpha-hydroxypregna-4-ene-3, 11, 20-triketone and introduce double bonds, the transformation rate is as high as 90%, the use of strong acid, strong alkali and toxic and harmful chemicals is avoided or reduced, the operation conditions are improved, the production cost is reduced, and the concept of green development is met.
Owner:HENAN LIHUA PHARMA
Mutant strains and engineering bacteria of Arthrobacter simplex with stress tolerance
The invention belongs to the field of genetic breeding, and in particular relates to a simple Arthrobacter mutant strain with stress tolerance and a genetic engineering bacterium constructed therefrom. Among them, the survival rate of the mutant strains under 16% ethanol and 20% methanol shock conditions increased by 3.74 times and 2.10 times respectively compared with the starting strain; overexpression of groEL, dnaK, recA, uvrD, katG, sod or treS genes from the mutant strains The tolerance of the engineered bacteria to high concentrations of organic solvents is significantly improved, and they also show higher tolerance to salt stress and oxidative stress. The above engineered bacteria are used in the steroid C1,2 dehydrogenation reaction to put The ethanol concentration was increased to 8-10%, the substrate CA concentration was increased to 6-8g / L, and the product yield was increased by 0.41-3.56 times, which laid a good foundation for the subsequent research on the resistance mechanism and the molecular transformation and application of strains.
Owner:TIANJIN UNIV OF SCI & TECH
Method for preparing betamethasone intermediate
ActiveCN110066846ASimple processReduce cost pressureMicroorganism based processesFermentationBetamethasoneArthrobacter simplex
The invention provides a method for preparing a betamethasone intermediate. By using one of a single strain Arthrobacter simplex CPCC 140451 or a single strain Nocardioide simplex ACCC 10205, the hydrolysis and dehydrogenation processes of the compound I are conducted in a same system, and it is achieved that beta methyl epoxide is obtained through one-step fermentable conversion. The technology is simple, and the method is suitable for industrial production.
Owner:ZHEJIANG XIANJU PHARMA
Arthrobacter simplex engineered strain with excellent stress tolerance and construction method and application thereof
PendingCN111254143AIncreased resistance to organic solventsExcellent stress toleranceBacteriaMicroorganism based processesHigh concentrationOrganic solvent
The invention relates to an arthrobacter simplex engineered strain with excellent stress tolerance and a construction method and application thereof. The arthrobacter simplex engineered strain is obtained by adopting a promoter engineering method, a global transcription mechanism engineering method or a method combining the promoter engineering and the global transcription mechanism engineering. The strain still keeps good activity in a transformation system with high concentrations of organic solvents (8%) and substrates (15-45 g / L), with the product yield in a C1,2 (subscript) dehydrogenation reaction being 1.1-2.2 times higher than a control strain I (containing a wild-type promoter and a wild-type irrE). According to the construction method and the application of the engineered strainwith excellent stress tolerance, the concentrations of organic solvents and substrates in the transformation system are increased, and then the product production is increased. The invention has important guiding significance for improving the transformation efficiency of the high-concentration substrates of steroid hydrophobic compounds.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Genetically engineered bacteria and starter for the dehydrogenation of the 1 and 2 positions of the a ring of steroidal compounds
InactiveCN103255095BImprove biotransformation efficiencyBacteriaMicroorganism based processesBiotechnologyPhosphate
The invention relates to a genetically engineered bacterium and a leavening agent for dehydrogenation of steroid A ring 1,2 positions. The host bacterium of the genetically engineered bacterium is Arthrobacter simplex, the preservation number of which is ACCC 12070. The genetically engineered bacterium contains a recombinant gene fragment as shown in Sequence 2. The fermentation substrate of the genetically engineered bacterium contains cortisone acetate, fluoride cortical hormone and 4-AD. The constructed engineered bacterium provided by the invention performs fermentation by constant temperature ventilation stirring on the basis of yeast extract, phosphate, corn steep liquor and glucose containing nutritional ingredients. When the concentration of steroids is not higher than 10%(m / V), one time addition of steroid substrate is adopted, and?biological dehydrogenation conversion rate can reach to more than 95% in?32-48 hours. When the concentration of steroids is higher than 10%, continuous addition of steroid substrate is adopted, and biological dehydrogenation conversion rate can reach to more than 95% within 60 hours.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for producing prednisolone acetate
InactiveCN101210259BStrong response specificityEasy to operateMicroorganism based processesFermentationMicrobial transformationArthrobacter simplex
Owner:TIANJIN UNIV OF SCI & TECH
A kind of method for preparing betamethasone intermediate
ActiveCN110066846BSimple processReduce cost pressureMicroorganism based processesFermentationBiochemical engineeringArthrobacter simplex
Owner:ZHEJIANG XIANJU PHARMA
Method for producing androstane dienedione through fermentation of arthrobacter simplex
PendingCN114292892ASuitable for industrial applicationsBacteriaMicroorganism based processesBiotechnologyPharmaceutical drug
The invention belongs to the technical field of preparation of steroid hormone drugs, and particularly relates to a method for producing androstane dienedione by fermentation of arthrobacter simplex, which mainly comprises the following steps: optimizing fermentation culture medium components, feeding points and substrates for whole-cell transformation of arthrobacter simplex to obtain an ADD crude product with high conversion rate, and extracting and refining by mixing acetone and water to obtain the androstane dienedione. And an ADD fine product is obtained. The method effectively solves the problems that in the prior art, the conversion efficiency is not high, byproducts are difficult to separate, the process is tedious and not suitable for industrial mass production, and the like, the fermentation feeding concentration is increased to 30-60 g / L, and the conversion rate is increased to 97% or above.
Owner:ZHEJIANG XIANJU PHARMA
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