37 results about "Arthrobacter simplex" patented technology

The invention provides a method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium, relating to a method for preparing a compound II by adopting 4-alkene-3,20-dione steroid I as an initiator through microbial co-fermentation. The method is characterized in that the 11beta-hydroxyl-1,4-diene-3,20-dione steroid II is prepared through the mixed one-step fermentation of curvularia lunata AS 3.4381 and arthrobacter simplex AS1.94.

The invention discloses a method for preparing a dexamethasone epoxy hydrolysate 8DM from arthrobacter simple CPCC140451 through one-step fermentation. The method is simple in operation manner, stablein process and suitable for industrial mass production.

The invention discloses a method for preparing 16,17[alpha]-epoxyprogesterone by virtue of a biological process. By conducting biological fermentation on a 3-hydro-5-ene-16,17[alpha]-epoxy substance by virtue of autologous cholesterol oxidase of Arthrobacter simplex AS1.94, so that biological manufacturing of the 16,17[alpha]-epoxyprogesterone is achieved; therefore, a conventional chemical process is replaced and the conversion rate and yield of the 16,17[alpha]-epoxyprogesterone are improved, wherein the 3-hydro-5-ene-16,17[alpha]-epoxy substance serves as a substrate and the Arthrobacter simplex AS1.94 is a catalysis strain; and by conducting the fermentation, the yield of the 16,17[alpha]-epoxyprogesterone is greater than 90%, and the purity of the 16,17[alpha]-epoxyprogesterone is greater than 98.5%.

The invention belongs to the field of genetic breeding, and in particular relates to a simple Arthrobacter mutant strain with stress tolerance and a genetic engineering bacterium constructed therefrom. Among them, the survival rate of the mutant strains under 16% ethanol and 20% methanol shock conditions increased by 3.74 times and 2.10 times respectively compared with the starting strain; overexpression of groEL, dnaK, recA, uvrD, katG, sod or treS genes from the mutant strains The tolerance of the engineered bacteria to high concentrations of organic solvents is significantly improved, and they also show higher tolerance to salt stress and oxidative stress. The above engineered bacteria are used in the steroid C1,2 dehydrogenation reaction to put The ethanol concentration was increased to 8-10%, the substrate CA concentration was increased to 6-8g / L, and the product yield was increased by 0.41-3.56 times, which laid a good foundation for the subsequent research on the resistance mechanism and the molecular transformation and application of strains.

The invention belongs to the field of microbial pharmaceutics and pharmaceutical engineering, specifically relates to a production method of prednisone acetate by microbial transformation with Arthrobacter simplex as bacteria strain and hydrocortisone acetate as substrate. The method uses Arthrobacter simplex as bacteria strain and comprises the following steps of: performing primary seed culture, performing second fermentation culture, adding hydrocortisone acetate into the fermentation liquid of Arthrobacter simplex to transform hydrocortisone acetate into prednisone acetate, filtering, andcollecting cake to obtain prednisone acetate. The bacteria can be prepared into double liquid phase, broken cells or protoplast, each of which has high transformation ratio. The inventive production method replaces cortisone acetate with hydrocortisone acetate as raw material, and has the advantages of high yield, simple process, good economical and practical performance, and less use of harmful reagents; and is important for steroids production with biotransformation method.

The invention provides a method for preparing a betamethasone intermediate. By using one of a single strain Arthrobacter simplex CPCC 140451 or a single strain Nocardioide simplex ACCC 10205, the hydrolysis and dehydrogenation processes of the compound I are conducted in a same system, and it is achieved that beta methyl epoxide is obtained through one-step fermentable conversion. The technology is simple, and the method is suitable for industrial production.