63results about How to "High bacterial content" patented technology

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

The invention discloses bacteria immobilization particles for water purification and a preparation method of the bacteria immobilization particles, and belongs to the technical field of water treatment. The preparation method comprises the following steps: preparing a bacteria concentrate through flocculation by utilizing activated carbon, diatomite and a chitosan acetic acid solution; adding diatomite, bentonite, silicon dioxide and a polyvinyl alcohol-sodium alginate mixed solution into the concentrate for stirring; carrying out granulation with a granulator to prepare the bacteria immobilization particles. The prepared bacteria immobilization particles are high in biological activity, high in bacteria content (5*10<9>-1*10<10> CFU/g), short in starting time, and high in reaction speed, has a honeycomb structure (100-300 m<2>/g in specific surface area) suitable for bacteria apposition growth and mass transfer, and can be used for immobilizing bacteria for long, prohibiting the invasion of external hazardous materials, and permanently and efficiently treating sewage in different environments and of different water quality types; meanwhile, industrialized production is available for a whole bacteria immobilization particle preparation process, and the prepared bacteria immobilization particles are high in mechanical property, long in service life, and convenient to store and convey.

The invention relates to a water purification microbial inoculum which is produced by blending and fermenting a plurality of bacteria. Straws are taken as raw materials and then seed solutions of a plurality of bacteria, a straw fermention medium and a concentrated nitrifying bacteria solution are added, thus obtaining the water purification microbial inoculum. A preparation method of the water purification microbial inoculum comprises the steps as follows: the straws are sent to a material steam explosion system and then a steam explosion is conducted to the straws; after the steam explosion, the straws are sent to a chelated reaction storage for reaction; after reacting in the chelated reaction storage, the straws are sent to a fermention storage for fermentation; 5 percent of the seed solution of a plurality of sporos and 30 percent to 40 percent of the straw fermention medium are added to the fermention storage; and before the straws are taken out of the storage, 2 percent to 3 percent of the concentrated nitrifying bacteria solution is added and stirred evenly, thereby obtaining the water purification microbial inoculum. The water purification microbial inoculum has the advantages that the valid viable bacteria number of the produced water purification microbial inoculum reaches 2 billion/g (slab) and nitration and subnitration can be realized; a purifier has comprehensive water purification functions which can effectively restrain the harmful bacteria of a cultured water body, thereby realizing the disease prevention and survival rate improvement; the degradation of the organic pollutant in the water body can be accelerated; and ammonia nitrogen can be decreased and dissolved, and oxygen can be increased, thereby achieving the aims of increasing production and income.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention relates to a paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation, a preparation method, and applications thereof. The pesticide-fertilizer comprises the following raw materials: paenibacillus polymyxa powder, colistin sulfate, natamycin, L-alanine, valine, yeast powder, corn steep liquor powder, disodium hydrogen phosphate, sodium chloride, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and valine) can rapidly promote the germination of spores of paenibacillus polymyxa and improve the germination rate of spores. The provided pesticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve product quality, and can be used as a microbial pesticide, and the effect is stable.

The invention relates to a bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation, a preparation method and applications thereof. The insecticide-fertilizer comprises the following raw materials: bacillus amyloliquefaciens, colistin sulfate, natamycin, L-alanine, asparagine, yeast extract powder, peptone, disodium hydrogen phosphate, sodium chloride, glucose, fructose, and brown sugar. The colistin sulfate and natamycin in the insecticide-fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (alanine, fructose, and asparagine) can rapidly promote the germination of spores of bacillus amyloliquefaciens and improve the germination rate of spores. The provided insecticide-fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The insecticide-fertilizer can prevent disease, promote growth, and increase output and improve quality, and can be used as a microbial pesticide, and the effect is stable.

The invention discloses a special biocontrol compound microbial fertilizer for grapes. An organic raw material is amino acid hydrolysis residue, and a bacterial agent is a mixed bacterial agent of bacillus subtilis and bacillus amyloliquefaciens; the strain in the bacterial agent is subjected to factory fermentation; the initial pH value of the culture medium is 6.5-7.5, and the fermentation temperature is 30-35 DEG C; and a nitrogen source comprises 140.0-106.0g/L corn flour, 0.6-1.3g/L glucoamylase, 1.2-1.8g/L alpha-amylase, 4.0-6.5g/L KH2PO4 and 3.0-6.0g/L CO(NH2)2. The invention also discloses a production method of the compound microbial fertilizer. In the special compound microbial fertilizer for grapes, disclosed by the invention, the microorganisms and the fertilizer enter the soil at the same time and are colonized at the root of a grape plant, and the growth of pathogenic bacteria of coniothyrium diplodiella is inhibited so as to reduce the morbidity of coniothyrium diplodiella.

The preparation process of acidophilic lactobacillus milk tablet adopts inducing mutation breeding method to breed high temperature mutant acidophilic lactobacillus strain, i. e., acidophilic lactobacillus LA201 strain. Meanwhile, matter capable of lowering the death rate of acidophilic lactobacillus is extracted from the Chinese herbal medicine extract liquid and microbe culture liquid. The breeding of high temperature acidophilic lactobacillus strain and the protecting agent make it possible to produce acidophilic lactobacillus milk tablet in available milk powder producing high temperature spray drying apparatus and conventional tabletting.

The invention relates to the technical field of water treatment, particularly a microbial water purifying agent. The microbial water purifying agent is prepared from a culture medium and a liquid microbial inoculant. The culture medium is composed of the following components in parts by mass: 50-70 parts of rice hull, 8-15 parts of bagasse, 15-30 parts of wheat bran, 5-10 parts of bean pulp, 5-15 parts of peanut pulp, 2-6 parts of sodium dihydrogen phosphate, 0.2-0.8 part of sodium chloride, 0.5-1.2 parts of trace element solution, 3-8 parts of rock powder, 5-20 parts of glucose, 2-6 parts of ammonium chloride solution, 1.2-5.8 parts of beef leaching substance and 2-6 parts of yeast extract. By adopting the combination of multiple bacteria and using mixed fermentation, the microbial water purifying agent can well remove nitrogen, phosphorus and other eutrophic substances in water, can effectively inhibit other harmful bacteria in the water body, achieves the effects of preventing diseases and enhancing the survival percent, and achieves the goal of water purification.

The invention provides a production method of a nonpathogenic fusarium oxysporum-containing plant vaccine preparation. The method comprises the following steps: preparing liquid strain for production by taking nonpathogenic fusarium oxysporum FJAT-9290 as strain and using a potato dextrose agar (PDA) culture medium and a Czapek yeast culture solution as a culture medium for the liquid strain; preparing a solid culture medium substrate for plant vaccine production by taking pig manure waste as a main component, mixing the prepared solid culture medium substrate with bran and wheat grains, respectively packaging the mixture into polypropylene plastic bags, sterilizing and then inoculating the liquid strain, culturing and fermenting for 18-20 days at the constant temperature of 28-30 DEG C to prepare a solid fermentation product of a plant vaccine, and evenly mixing the product after fermentation to obtain the nonpathogenic fusarium oxysporum-containing plant vaccine. The plant vaccine preparation prepared by the method has effects of promoting growth of crops and resisting diseases, can solve the problem that tomato fusarium wilt is difficult to control, and achieves the aim of reducing the use of chemical pesticides.

The invention discloses a semisolid fungus cultivation method for nitrifying bacteria. The cultivation method comprises the following steps: 1, respectively inoculating purified ammonia oxidizing bacteria and nitrite oxidizing bacteria into triangular bottles, and putting the triangular bottles in a shaking bed for 12 days' heat-insulating cultivation; 2, respectively inoculating the strains into corresponding seed tanks, pumping gas into the seed tanks, stirring, and carrying out cultivation for 12 days; 3, mixing the strains with 99 times of corresponding culture solutions, mixing the mixtures with 3 times of carriers to obtain semisolids, putting the semisolids into containers for 20 days' heat-insulating and moisture-holding cultivation; step 4, heating the containers, and removing the moisture in the cultivated semisolids with exhaust fans, and smashing the dried cultivated semisolids; step 5, mixing the two semi-finished products according to the proportion of 1:1, and mixing uniformly to obtain the finished product. The semisolid fungus cultivation method is easy to operate, low in production cost, stable in product quality, and has the advantage that the finished product contains 0.2 billion or more bacteria per gram, and is easy to store and transport.

The invention provides a bio-organic fertilizer prepared by combining dead bacillus vallismortis and a straw organic fertilizer through secondary solid fermentation, and a preparation method and application thereof. The content of dead bacillus vallismortis in the bio-organic fertilizer after the secondary solid fermentation is finished is taken as a detection index, the optimal culture condition combination of the bio-organic fertilizer is obtained by screening through a single-factor method and a multi-factor and multi-level orthogonal method, and the fermentation process of the bio-organic fertilizer is optimized. The BIO-4 bio-organic fertilizer, which is researched and developed by taking a corn straw organic fertilizer as a matrix and taking dead bacillus vallismortis SZ-4 as a biocontrol bacterium source, has a good prevention and control effect on root rot caused by fusarium solani, has a promoting effect on crop growth, and is beneficial to increase of crop yield and improvement of quality.

The invention discloses a method for preparing a viable bacillus subtilis preparation for feed. The viable bacillus subtilis preparation for the feed is prepared by the following steps of: (1) performing activation culture on bacillus subtilis strains; (2) mutagenizing and screening the activated bacillus subtilis strains; (3) performing propagation culture on the screened bacillus subtilis strains; (4) preparing bacillus subtilis liquid from the bacillus subtilis strains subjected to the propagation culture; (5) mixing the bacillus subtilis liquid and an absorption carrier, wherein the absorption carrier is a mixture of maize alcohol lees and maize cob meal; and (6) pneumatically drying the bacillus subtilis liquid-absorbed carrier to obtain the finished product. By the method, the low-cost and stable-quality viable bacillus subtilis preparation can be prepared.

The invention provides a high density fermentation method of a genetically engineered bacterium of recombinant interferon, and belongs to the field of fermentation engineering. The high density fermentation method comprises following steps: a genetic engineering seed stored in liquid nitrogen after construction recombination is subjected to activation, shaking culturing, and amplification culturing, and is delivered into a fermentation cylinder for aeration stirring culturing, when bacterium OD600 is increased to be about 5, an inducing agent is added; ingredient supply, stirring speed, and aeration amount are adjusted based on a bacterial strain growth curve, nutrient requirements, and pH change in the fermentation cylinder; and discharging is carried out when conversion from growth period into decline phase is realized, and crude purification and condensation are carried out, so that a crude enzyme product is obtained. According to the high density fermentation method, optimization of dissolved oxygen amount, fermentation medium, medium supply, and supplying method is realized, so that nutrient conditions and external environment required by rapid growth and metabolisms of bacteria are ensured, bacterium content and product yield in fermentation cylinder discharging are increased greatly, and bacterium content is increased from 25g/L to 45g/L.