Bio-organic fertilizer containing dead bacillus vallismortis, and preparation method and application thereof

A technology of Bacillus dead valley and bio-organic fertilizer, which is applied in the field of bio-organic fertilizer containing Bacillus dead valley and its preparation field, can solve problems such as rare reports of biological fertilizers, and achieves improved prevention and control efficiency, good control effect, and improved plant growth. The effect of quality and yield

Active Publication Date: 2021-08-06
JILIN AGRICULTURAL UNIV
View PDF13 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacterial strain is now preserved in the China General Microorganism Culture Collection Management Center (preservation number is CGMCC No.8273), and the application name is "a kind of Bacillus dead valley and its application", and the authorized announcement number is an invention patent of CN103952329B, but for Reports of biofertilizers containing this strain are rare

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bio-organic fertilizer containing dead bacillus vallismortis, and preparation method and application thereof
  • Bio-organic fertilizer containing dead bacillus vallismortis, and preparation method and application thereof
  • Bio-organic fertilizer containing dead bacillus vallismortis, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Bacillus vallismortis SZ-4 was preserved by the State Key Laboratory of Ecological Restoration and Ecosystem Management jointly established by Jilin Agricultural University.

[0045] The activation of Bacillus death valley SZ-4 uses beef extract peptone medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 16g, deionized water 1000mL, pH 6.8-7.0); the preparation of fermented seed liquid uses beef Extract-peptone culture medium (beef extract-peptone culture medium without agar); the preparation of the fermented liquid adopts BPY fermented liquid (beef extract 5g, peptone 10g, yeast extract 5g, glucose 5g, NaCl 5g, deionized water 1000mL, pH 6.8-7.0).

[0046] Preparation of fermentation broth of strain SZ-4: activate the Bacillus dead valley SZ-4 test tube species in beef extract peptone medium, culture at a constant temperature of 37°C for 24 hours with shaking, pass for 3 times, pick a single colony and inoculate it in beef extract peptone medium , 32°C, 180r / min...

Embodiment 2

[0070] Preparation of fermentation broth of strain SZ-4: activate the Bacillus dead valley SZ-4 test tube species in beef extract peptone medium, culture at a constant temperature of 37°C for 24 hours with shaking, pass for 3 times, pick a single colony and inoculate it in beef extract peptone medium , 32°C, 180r / min shaking culture for 24h, to obtain SZ-4 bacterial seed liquid. Put the seed liquid into the BPY fermentation liquid according to the inoculum amount of 5%, shake and culture at 32°C and 180r / min for 48h, and adjust the bacterial content to about 10 by blood counting method. 8 CFU / mL, to obtain the Bacillus dead valley SZ-4 fermentation broth.

[0071] Calculation of bacteria content: using the hemocytometer method. Spot 10 μL of bacterial fermentation broth on the hemocytometer, record the bacterial cells in the middle, upper left, lower left, upper right, and lower right of the hemocytometer, and number them sequentially as a 1 , a 2 , a 3 , a 4 , a 5 , bac...

Embodiment 3

[0074] Preparation of BIO-4 Biological Organic Fertilizer

[0075] BIO-4 biological organic fertilizer is produced by secondary solid fermentation of Bacillus dead valley SZ-4 fermentation liquid and corn stalk organic fertilizer. The preparation method is as follows:

[0076] With SZ-4 bacterium fermented liquid (10 8 CFU / mL) was inoculated in the organic matrix in Example 1 according to the inoculum amount of 10%, adjusted the water content to 45%, added urea until the C / N was 15, then placed it in a sterile plastic box, and cultivated it at a constant temperature of 32°C for 4 days. Turn over once every 48h. Gained bio-organic fertilizer is named as BIO-4 bio-organic fertilizer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a bio-organic fertilizer prepared by combining dead bacillus vallismortis and a straw organic fertilizer through secondary solid fermentation, and a preparation method and application thereof. The content of dead bacillus vallismortis in the bio-organic fertilizer after the secondary solid fermentation is finished is taken as a detection index, the optimal culture condition combination of the bio-organic fertilizer is obtained by screening through a single-factor method and a multi-factor and multi-level orthogonal method, and the fermentation process of the bio-organic fertilizer is optimized. The BIO-4 bio-organic fertilizer, which is researched and developed by taking a corn straw organic fertilizer as a matrix and taking dead bacillus vallismortis SZ-4 as a biocontrol bacterium source, has a good prevention and control effect on root rot caused by fusarium solani, has a promoting effect on crop growth, and is beneficial to increase of crop yield and improvement of quality.

Description

technical field [0001] The invention relates to the technical field of fertilizer production, in particular to a bio-organic fertilizer containing Bacillus dead valley and its preparation method and application. Background technique [0002] Ginseng Panax ginseng C.A. Meyer is a famous and precious traditional Chinese medicine, known as the "King of Herbs", and is widely planted in China and South Korea. my country is a big country of ginseng planting. Due to the introduction of policies such as "returning farmland to forests", the traditional "cutting down forests and planting ginseng" has been restricted, and the ginseng cultivation mode in my country has gradually transformed into farmland planting ginseng. However, due to immature ginseng planting technology and frequent occurrence of soil-borne diseases, the quality and yield of ginseng cannot be guaranteed, seriously affecting the competitiveness of my country's ginseng industry in the international market. Therefore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05F15/00C05F17/20C12N1/20C12R1/07
CPCC05F11/08C05F11/00C05F17/00C12N1/20Y02W30/40
Inventor 杨利民韩梅狄平孙卓张永刚林红梅吕泽良
Owner JILIN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap