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A kind of Lactobacillus acidophilus freeze-drying protective agent

A technology of Lactobacillus acidophilus and seeds, applied in the direction of bacteria, preserved microorganisms, microorganisms, etc., can solve the problems of easy inactivation, etc., and achieve the effects of high bacterial content, convenient preservation and convenient use

Active Publication Date: 2016-09-14
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, as a probiotic, Lactobacillus acidophilus is easily inactivated before production, storage and entering the intestinal tract. In order to obtain higher live bacteria, it needs to be protected. Therefore, the protection technology of Lactobacillus acidophilus has become Its application to core technical issues in animal production

Method used

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  • A kind of Lactobacillus acidophilus freeze-drying protective agent
  • A kind of Lactobacillus acidophilus freeze-drying protective agent
  • A kind of Lactobacillus acidophilus freeze-drying protective agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The seed liquid preparation of embodiment 1 Lactobacillus acidophilus

[0025] Activation culture of Lactobacillus acidophilus: Streak the strain Lactobacillus acidophilus CGMCC No.5093 preserved in the glycerol cryopreservation tube on the MRS agar medium plate with an inoculation needle, and culture at a constant temperature of 37°C for 20 hours. Then inoculate the single colony grown on the plate into a 250mL Erlenmeyer flask containing 100mL MRS liquid medium, and incubate at a constant temperature of 37°C for 20h. The cultured bacterial solution was streaked on the MRS medium plate again, and after 20 hours of constant temperature cultivation at 37°C, a single colony was inoculated into a 250mL Erlenmeyer flask containing 100mL of MRS liquid medium, and incubated at 37°C for 20h. The above operation was repeated 3 times to complete the activation of Lactobacillus acidophilus.

[0026] Preparation of seed solution of Lactobacillus acidophilus: inoculate a single co...

Embodiment 2

[0029] The effect comparison of embodiment 2 different materials to Lactobacillus acidophilus freeze-drying protection

[0030] 1. Experimental materials

[0031] (1) Suspension matrix: MRS enrichment liquid MRS liquid medium, sterilized at 121°C for 15 minutes.

[0032] (2) Tested strain: Lactobacillus acidophilus CGMCC No.5093.

[0033] (3) Protective agent materials: sucrose, lactose, skimmed milk powder, D-erythorbic acid, corn starch, sodium glutamate, trehalose, sorbitol, glycerin.

[0034] (4) Feed: 50% glucose, sterilized at 121° C. for 15 minutes.

[0035] (4) Colony count medium: MRS agar medium.

[0036] (5) Vacuum freeze dryer: German CHRIST ALPHA2-4LSC type.

[0037] 2. Freeze-drying of Lactobacillus acidophilus

[0038] 2.1 Proliferation and cultivation of Lactobacillus acidophilus

[0039] Inoculate 300mL of the cultured seed solution into a 5L fermenter with 4L of optimized medium. The culture parameters are: temperature, 37°C; stirring speed, 50rmp; pH v...

Embodiment 3

[0058] Embodiment 3 best protective agent optimization formula design

[0059] According to the mechanism of bacterial frostbite and cryoprotection, combined with the protective effect and function characteristics of each protective agent, the source and properties of the protective agent, it was studied that trehalose, sucrose, skimmed milk powder, D-isoascorbic acid, and sodium glutamate were used as acidophilic milk. Optimal formulation of Bacillus freeze-drying protectant composition.

[0060] After determining the level of each protective agent, an orthogonal experiment of 5 factors and 4 levels of L1654 was designed (see Table 2) to optimize the best combination of Lactobacillus acidophilus lyoprotectant. See Table 2 for the factor levels of the orthogonal experiment, and Table 3 for the experimental design and implementation plan.

[0061] Table 2 Orthogonal experiment factor level table

[0062]

[0063] Table 3 Experimental design and implementation plan

[0064...

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Abstract

The invention discloses lactobacillus acidophilus cryoprotectant, which contains 1.0 to 5.0 percent of trehalose, 5.0 to 20.0 percent of defatted milk powder, 2.0 to 8.0 percent of cane sugar, 0.4 to 1.0 percent of D-isoascorbic acid and 0.5 to 2.0 percent of monosodium glutamate. The lactobacillus acidophilus cryoprotectant has the advantages of small size, high bacteria content, low pollution, convenience in preservation, transportation and use and the like. The viable count of the actobacillus acidophilus dry powder prepared by utilizing the lactobacillus acidophilus cryoprotectant can reach more than 4.0*10<11> CFU / g.

Description

technical field [0001] The invention specifically relates to a Lactobacillus acidophilus freeze-dried protective agent and a Lactobacillus acidophilus freeze-dried agent containing the same, belonging to the technical field of probiotic preparations. Background technique [0002] Lactobacillus acidophilus is an inherent beneficial bacterium in animals. It and Bifidobacteria account for more than 95% of the probiotics in the intestinal tract of animals. They mainly promote the health of animals by maintaining the micro-ecological balance of the intestinal tract. Lactobacillus acidophilus mainly exists in the upper part of the intestinal tract, and maintains the health of the entire intestinal tract together with Bifidobacteria, which mainly exists in the lower part. Taken together, it has the following effects: [0003] (1) Improving immunity: Lactobacillus acidophilus can produce a large amount of immunoglobulin, thereby activating the collective immune cells, making it dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/23
Inventor 胡婷王建设刘滢
Owner BEIJING DABEINONG TECH GRP CO LTD
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