37 results about "MRS agar" patented technology

The invention discloses lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550, belonging to the technical field of biology. The lactobacillus plantarum 550 provided by the invention is preserved in the China Center for Type Culture Collection on December 21, 2007, wherein the accession number of the lactobacillus plantarum 550 is CCTCC No. M 207202. The lactobacillus plantarum 550 provided by the invention is applied to preparation of functional food and medicines for relieving constipation, or / and helping sleep, or / and reducing cholesterol, or / and inhibiting pathogenic bacteria, or / and degrading nitrite. The lactobacillus plantarum 550 grows well and quickly on an MRS agar culture medium, is high in acid production capacity, has good gastric acid and cholate tolerance, and can be colonized in intestinal tracts. The lactobacillus plantarum 550 has a good bowel relaxing effect, strong capability of producing gamma-aminobutyric acid, the effect of reducing cholesterol in vitro, a good inhibition effect on common pathogenic bacteria, capability of rapidly degrading nitrite and the good safety.

The invention discloses an industrial production method for dried sweet potato leaf products. The method comprises the following operating steps: screening sweet potato leaves, rinsing with clean water, fixing green and protecting green, marinading and fermenting, separating the leaves and size, drying and dewatering. The method is technically characterized by comprising the steps: marinading andfermenting, namely inoculating lactobacillus plantarum onto an MRS agar medium to be activated, and culturing at a temperature of 33-37 DEG C for 2-3 days; inoculating the activated culture onto sweetpotato leaves in a fermentation tank according to an inoculum size of 3%, adding 3% of salt, uniformly stirring, and sealing the fermentation tank, wherein the fermentation temperature is 30-32 DEG C, and the fermentation time is 2-3 days; drying and dewatering, namely performing forced air drying at the temperature of 45-50 DEG C for 4-5 hours. Constitution water of the sweet potato leaves is not damaged as far as possible, and the original physical, chemical and biological properties and shapes invariable are kept unchanged. According to the method disclosed by the invention, the sweet potato leaves subjected to lactic acid fermentation are well flavored, contain favor components such as lactic acid, amino acids, fatty acids and the like, can achieve the effects of removing the own tannic acid and improving the product flavor and palatability, and are convenient to store, transport and sell after drying and dewatering.

The invention relates to microorganisms and meat food fermentation, and specifically discloses a plant lactobacillus and its application in fermenting and preparing Sichuan sausages. The Lactobacillus plantarum 1-1 provided by the present invention is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, with a preservation number: CGMCC NO.15467, and a preservation date: March 20, 2018. The strain was isolated from traditional sausages in Mount Qingcheng, Dujiangyan City, Sichuan Province. It grew well on MRS agar medium. The main fermentation characteristics met the requirements of meat starter and were suitable for meat fermentation. It has the ability to degrade sarcoplasmic proteins and biogenic amines in an in vitro simulation system. Applied to the production of Sichuan sausage, it can promote the degradation of sarcoplasmic protein in Sichuan sausage and significantly reduce the content of biogenic amines in Sichuan sausage. It has the potential to be applied to the actual production of Sichuan sausage, thereby ensuring and improving the flavor, taste and safety of Sichuan sausage.

The invention relates to an enterococcus faecalis with the collection number of CGMCC No.3164. A bacterial colony is cultured on an MRS agar culture medium at the temperature of 37 DEG C for 24 hours to be gathered; the bacterial colony is white, thallus is ovate, extends along chain direction, is in pair or in short chain, is Gram-negative, generates lactic acid when fermenting glucose and is elliptic under a common microscope. When being applied, the bacterial colony is inoculated into a test tube filled with culture medium A to stand at the temperature of 37 DEG C to be cultured for 24 hours; after being repeatedly activated five times, the bacteria solution is inoculated into a culture medium B to stand at the temperature of 37 DEG C to be cultured for four days; the cultivated bacteria solution is decentralized at 1000 rpm for 10 minutes; and supernate is extracted to obtain high concentration tyramine solution. The culture medium A is an MRS liquid culture medium of 0.1% of tyrosine, and the culture medium B is an MRS liquid culture medium of 0.005% of pyridoxal phosphate and 0.1% of tyrosine.

The invention belongs to the field of biotechnology and provides a strain of Lactobacillus reuteri and its application in the bee breeding process. Lactobacillus reuteri was preserved on July 2, 2018 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.16043, and the classification is named Lactobacillus reuteri; the Lactobacillus reuteri After cultured on MRS agar medium, the colony was round, milky white, with neat edges, smooth surface and raised, opaque. The Lactobacillus reuteri LP4 was independently isolated from the intestines of worker bees of Apis mellifera Apis mellifera, which were raised by beekeepers in Luoping County, Yunnan Province, and freely fed on rapeseed nectar in spring. It has good antibacterial activity and is sensitive to various antibiotics. Press Lactobacillus reuteri LP4 by 10 8 The amount of CFU / mL added to the sugar water fed to bees can improve the micro-ecological structure of the flora in the bee intestines, enhance the intestinal immunity of bees, and increase the survival rate of bees.

The invention discloses a strain of Lactobacillus fermentum 9-4 and its application. The strain was deposited in China Center for Type Culture Collection CCTCC on August 12, 2019, and the preservation number is CCTCC NO: M 2019619. The Lactobacillus fermentum is a lactic acid bacteria strain with purine-reducing ability screened from raw rice flour, a traditional food in Guangxi Zhuang area. The strain grows well on MRS agar medium and has strong acid resistance. The post-survival rate is 34%; it can grow slowly under 3% concentration of bile salt, and the survival rate is 65%. The strain has good acid resistance, bile salt resistance, and purine-reducing ability, and is suitable for the production of low-purine food and purine-reducing functional fermented food.

The invention discloses a caries-resistant plant lactobacillus ( Lactobacillus plantarum ) K41 and its applications. The Lactobacillus plantarum K41 provided by the present invention was preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, with a preservation number of CGMCC No. 15462 and a preservation date of March 20, 2018. The strain was isolated from farmhouse-made kimchi in Yan'an Road, Shunqing District, Nanchong City, Sichuan Province. It grew well on MRS agar medium and could inhibit the growth of Streptococcus mutans UA159 cells, exopolysaccharide formation, hydroxyapatite degradation, and biofilm It has good self-polymerization and copolymerization ability, good tolerance to antibiotics, salt and acid, and is easy to colonize in the oral cavity. Animal experiments have shown that K41 can inhibit the demineralization of rat tooth enamel and the degree of dental caries, and its application in functional foods is of great significance to human health.

The invention discloses an Enterococcus faecium XC2 producing antibacterial substances and its screening method and application. The strain is named Enterococcus faecium XC2. The strain was preserved in the China Center for Type Culture Collection (CCTCC) on May 31, 2016. ), its deposit number is CCTCC NO: M2016297. The strain was screened from pig feces samples. On the KF Streptococcus agar medium, it was a red, smooth and raised colony with a neat periphery. On the MRS agar medium, it was a milky white colony with a smooth edge and a diameter of 1 mm. It was oval in microscopic examination. , single, double or short chain arrangement, Gram stain blue. The bacterial strain of the present invention has obvious inhibitory effect on Staphylococcus aureus, Salmonella and Escherichia coli, and it produces enterococcus B, which solves the problem of the disorder of intestinal flora of livestock and poultry caused by antibiotics, the decline of animal disease resistance and the unreasonable compliance with withdrawal period The problem of drug residues in animal products.

The invention relates to an enterococcus faecalis with the collection number of CGMCC No.3165. A bacterial colony is cultured on an MRS agar culture medium at the temperature of 37 DEG C for 24 hours to be gathered; the bacterial colony is white, oval, is in short chain and Gram-negative, generates acid when fermenting glucose and is elliptic under a common microscope. When being applied, the bacterial colony is selected from a slope to be inoculated into a test tube filled with culture medium A to stand at the temperature of 37 DEG C to be cultured for 24 hours; after being repeatedly activated five times, the finally activated bacteria solution is inoculated into a culture medium B to stand at the temperature of 37 DEG C to be cultured for four days; inoculation amount is 106cfu / ml; cultured bacteria solution is decentralized at 1000 rpm for 10 minutes; and supernate is extracted to obtain high concentration tyramine solution. The culture medium A is an MRS liquid culture medium added with 0.1% of tyrosine, and the culture medium B is an MRS liquid culture medium added with 0.005% of pyridoxal phosphate and 0.1% of tyrosine.

The invention provides a lactobacillus strain and its application, belonging to the technical field of microorganisms. The lactobacillus is preserved in the China Center for Typical Culture Collection, the preservation number is: CCTCC M 2017450, and the Latin name is Lactobacillus spicheri IZ. The colony characteristics of the Lactobacillus strain are: MRS agar plate, colony milky white, round, colony diameter is 2 mm ± 1 mm, slightly raised, moist, with neat edges, cell shape is rod-shaped, and the suitable growth temperature is 25 ° C ~ 35 ° C , is a facultative anaerobic strain. The lactobacilli are used to prepare the glutaric acid pepper, can significantly inhibit miscellaneous bacteria while retaining the main flavor of the original glutatic acid pepper, and reduce the defective rate of the glutaric acid pepper to 0%, and are suitable for industrial production of the glutaric acid pepper.

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus plantarum (L.plantarum) J-15 with the capability of inhibiting kidney stone formation and application thereof. The lactobacillus plantarum J-15 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22140, and the preservation date is April 6, 2021. The strain is separated from excrement of healthy people in Chengdu of Sichuan province, grows well on an MRS agar culture medium, has certain endurance capacity to acid and cholate, is sensitive to various antibiotics, has certain oxalate degradation capacity and relatively strong renal crystallization inhibition capacity, and is shown by whole genome sequencing to have relatively strong carbohydrate metabolism capacity, membrane transport capacity and environmental adaptability, is applied to the field of functional foods and clinical kidney stone prevention and adjuvant therapy, has actual production value and has very important significance to human health.

The invention relates to the technical field of microorganisms, and specifically discloses a Lactobacillus plantarum ( Lactobacillus plantarum ) N‑1 and its applications. The Lactobacillus plantarum N-1 provided by the present invention is preserved in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, with the preservation number: CGMCC NO.15463, and the preservation date: March 20, 2018. The strain was isolated from traditional yak cheese products in Kalong Village, Mula Township, Daocheng County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province. It grows well on MRS agar medium, has a certain tolerance to acids and bile salts, and is resistant to human colon cancer cells. Caco‑2 has high adhesion ability, strong cholesterol-lowering ability, and can significantly increase the production of intestinal short-chain fatty acids. It is not only of practical production value, but also of great significance to human health when applied in the field of functional foods.

The present invention relates to a bacteriocin-producing Enterococcus faecium strain. The preservation name of the strain is: Enterococcus faecium (Enterococcus faecium) R1. The preservation unit is: General Microbiology Center of China Microbiological Culture Collection Management Committee. The preservation date is January 2016. 20th, deposit number: CGMCC No.12085. The source of the strain is the traditional fermented soy bean paste from farmers. In the MRS agar medium, it was a round or oval white smooth opaque colony smaller than 1 mm; in the MRS liquid medium, it grew uniformly turbid; under the electron microscope, the bacteria were spherical, Arranged in pairs or chains, Gram staining was positive. The bacterial strain of the invention is sensitive to antibiotics, does not contain virulence genes, and belongs to safe bacterial strains. The bacteriocin produced by it is enterococcin P, which is stable under acidic conditions, has good thermal stability, and has good antibacterial effect on common intestinal pathogenic bacteria.