Lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550

A technology of Lactobacillus plantarum and constipation, which is applied to Lactobacillus plantarum 550 and its application fields, can solve the problems that have not been seen, and achieve the effect of rapid growth, strong acid production ability and good growth

Pending Publication Date: 2021-10-01
SICHUAN GAOFUJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the simultaneous production of γ-aminobutyric acid in the invention of probiotic strains for relieving constipation. Multifunctional probiotics such as sleep aids are of great significance to human health

Method used

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  • Lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550
  • Lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550
  • Lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Isolation, screening and molecular biology identification of embodiment 1 Lactobacillus plantarum 550

[0047] 1. Material preparation

[0048] The samples were collected from kimchi farms in Qingchengshan, Dujiangyan.

[0049] Cut the collected sample into pieces, weigh 1 g, and put it into 9 mL of sterile saline. After fully oscillating and mixing, 10-fold serial dilution was spread on MRS solid medium, and cultured at 37°C for 48h. Observe with the naked eye, pick single colonies of different shapes and sizes in the medium, and repeatedly streak and purify. It was initially identified as lactic acid bacteria by Gram staining and calcium dissolution method, and the purified strain was stored in 45% glycerol at -80°C for later use.

[0050] 2. Molecular biological identification of strains: the purified strains were sent to the Culture Collection Center of the Sichuan Provincial Microbial Resources Platform for 16S rRNA identification, and the measured 16S rRNA sequ...

Embodiment 2

[0056] Example 2 Growth ability test of Lactobacillus plantarum 550.

[0057] The 550 strain of Lactobacillus plantarum was inoculated into the MRS liquid medium according to the inoculum amount of 5%, activated and cultivated at 37°C for 24 hours, and activated twice continuously. Inoculate the activated 550 bacterium solution into the MRS liquid culture medium according to the inoculum size of 5%, and after mixing evenly, divide it into a sterile test tube (18mm×180mm test tube) by 8ml / branch; put the divided Lactobacillus plantarum Place 550 bacteria solution in a constant temperature incubator at 37°C for static culture, and take 3 test tubes to measure the absorbance value OD600 of the bacteria solution, and calculate the average value; at regular intervals, take 3 test tubes to measure the absorbance value OD600 of the 550 bacteria solution, and calculate the average value ; Take time as the abscissa and absorbance value OD600 as the ordinate to draw the growth curve.

...

Embodiment 3

[0059] Example 3 Acid-producing ability test of Lactobacillus plantarum 550.

[0060] The 550 strain of Lactobacillus plantarum was inoculated into the MRS liquid medium according to the inoculum amount of 5%, activated and cultivated at 37°C for 24 hours, and activated twice continuously. Inoculate the activated 550 bacteria solution into the MRS liquid medium at an inoculum size of 5%, and after mixing evenly, divide it into sterile test tubes (18mm×180mm test tubes) by 8ml / branch. Put 550 bacteria liquids packed in a constant temperature incubator at 37°C for static culture, and take 3 test tubes to measure the total acid of the bacterial liquid, and calculate the average value; at regular intervals, take 3 test tubes to measure the total acid of the bacterial liquid, And take the time as the abscissa and the acid production as the ordinate to draw the acid production curve.

[0061] The acid production curve is attached Image 6 shown. from Image 6 It can be seen that...

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Abstract

The invention discloses lactobacillus plantarum 550 with constipation relieving and sleep aiding functions and application of lactobacillus plantarum 550, belonging to the technical field of biology. The lactobacillus plantarum 550 provided by the invention is preserved in the China Center for Type Culture Collection on December 21, 2007, wherein the accession number of the lactobacillus plantarum 550 is CCTCC No. M 207202. The lactobacillus plantarum 550 provided by the invention is applied to preparation of functional food and medicines for relieving constipation, or / and helping sleep, or / and reducing cholesterol, or / and inhibiting pathogenic bacteria, or / and degrading nitrite. The lactobacillus plantarum 550 grows well and quickly on an MRS agar culture medium, is high in acid production capacity, has good gastric acid and cholate tolerance, and can be colonized in intestinal tracts. The lactobacillus plantarum 550 has a good bowel relaxing effect, strong capability of producing gamma-aminobutyric acid, the effect of reducing cholesterol in vitro, a good inhibition effect on common pathogenic bacteria, capability of rapidly degrading nitrite and the good safety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Lactobacillus plantarum 550 with functions of relieving constipation and helping sleep and its application. Background technique [0002] Constipation is a common clinical symptom, among which chronic constipation is the main symptom. Chronic constipation is mainly characterized by gastrointestinal dysfunction, manifested as difficulty in defecation and / or decreased frequency of defecation, and dry and hard stool. Difficulty defecation includes straining, difficulty passing stool, feeling of incomplete defecation, sensation of anorectal blockage, time-consuming defecation, and need for assisted defecation; reduced frequency of defecation refers to defecation less than 3 times per week. The duration of chronic constipation is at least 6 months. Long-term constipation will affect the quality of life and mental health of patients, and cause mental and psycholog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/10A61P25/20A61P3/06A61P31/04A23L33/135A23L2/02A23L2/38A23L2/39A23L19/20C12R1/25
CPCA61K35/747A61P1/10A61P25/20A61P3/06A61P31/04A23L33/135A23L2/02A23L2/382A23L2/39A23L19/20A23V2002/00A23V2400/169A23V2200/3204A23V2200/31A23V2200/3262A23V2200/30
Inventor 蒲小平刘梅钟综闾中平谢建将景晓青
Owner SICHUAN GAOFUJI BIOLOGICAL TECH
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