58results about How to "Easy to colonize" patented technology

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9>/mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention relates to a bacillus amyloliquefaciens plant subspecies ZFH-3 microbial agent and application thereof and belongs to the field of plant protection and disease prevention and treatment. The microbial agent per g contains 2*10<10>-1.2*10<11>cuf bacillus amyloliquefaciens plant subspecies ZFH-3 living spores, and the ZFH-3 strain is classified and named Bacillus amyloliquefaciens subsp.Plantarum, wherein the collection number is CGMCC No. 9884. The agent has a remarkable inhibiting effect on various pathogens of crops, shows a relatively good preventing and treating effect on prevention and treatment of soil-borne diseases and can effectively prevent and treat soil-borne diseases of vegetables, so that the loss is damaged and the field efficacy is over 59%. The microbial agent is non-toxic and safe to human bodies, replaces chemical agents for preventing and treating soil-borne diseases, conditioning soil and promoting plant growth, and has a good application prospect.

The invention discloses a bacillus subtilis bacterial strain and application thereof. The strain is bacillus subtilis Bs-1, is preserved in China Microbiological Collection Management Committee General Microbiology Center, and has a preservation number of CGMCC NO.12364. The invention further discloses application of a biological agent prepared by the bacillus subtilis Bs-1 to preparation and treatment of root-knot nematode. In the invention, bacillus subtilis is separated from soil, has an excellent control effect on root-knot nematode, is easy for colonization in the soil, and is beneficial to early prevention and control of the root-knot nematode hazard; the bacterial strain has a huge application potential for solving the problem of prevention and treatment of the root-knot nematode, and is helpful for implementing high yield, safety and pollution-free production of vegetables; fermentation liquor of the bacillus subtilis Bs-1 bacterial strain reaches a fatality rate of 100% on root-knot nematode, reaches a prevention and treatment effect of 76.9% in a greenhouse, has the good and stable prevention and treatment effect, and is suitable for agricultural production requirements.

The invention relates to the technical field of microorganisms, and discloses bacillus subtilis and application thereof. The preservation number of the bacillus subtilis is CGMCC No. 17357. The strainof the bacillus subtilis disclosed by the invention has a wide antibacterial spectrum; the inhibitory effect on fusarium wilt germs of multiple crops reaches up to 75% or above; moreover, the inhibitory effect on other 26 kinds of fungal pathogenic bacteria and 2 kinds of bacterial pathogenic bacteria is obvious; field planting in soil is easy; pesticide resistance is less liable to generate; thedurability of pesticide effect is good; the usage amount of pesticide can be effectively reduced; pollution-free production of the crops is facilitated; the expenditure of farmers is reduced.

The present invention relates to the technical field of microorganisms and particularly relates to a trichoderma strain isolated from an extremely arid desert environment, a cultivation method, a usemethod, a straw colonization method and an application thereof. The strain is trichoderma harzianum M-33 and preserved in China Center for Type Culture Collection. The cultivation method is as follows: the trichoderma harzianum M-33 is inoculated on a potato dextrose agar plate medium to be cultured in the dark. The trichoderma harzianum M-33 is applied in improving potato emergence rate, promoting potato plant growth and preventing and controlling potato black scurf. The use method is as follows: 190-210 parts by weight of trichoderma harzianum M-33 fermentation liquid is poured into a damp heat-sterilization wheat straw carrier to be fermented, then a fermented product is mixed with the soil, and the mixture is applied into a seeding ditch. A micro-ecology principle is used, straw is used as the carrier to assist colonization of the trichoderma strain in the soil, prevention and treatment effects of the trichoderma strain on potato soil-borne diseases are improved, and an applicationbasis for technology research of potato soil-borne disease biological prevention and control is provided.

The invention discloses walnut endophyte and a use thereof. The walnut endophyte is Bacillus amyloliquefaciens Ht-q6, is preserved in China general microbiological culture collection center in No.2, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Z. C. 100101 and has a preservation number of 9401. The walnut endophyte is separated and selected from walnut green peel, is Bacillus amyloliquefaciens and is named as Ht-q6. The walnut endophyte has antagonistic action on plant pathogenic fungi. The invention determines the optimal formula and fermentation conditions of the fermentation nutrient solution and provides a bacterial strain resource and a theoretical basis for development of an antibiological inoculants. The walnut endophyte is separated from plant tissue so that the endophyte easily colonize in plants and especially has stable effects of prevention and control of walnut diseases.

The invention relates to a multifunctional composite probiotic preparation as well as a preparation method and the application thereof. The multifunctional composite probiotic preparation is prepared from lactobacillus plantarum MiuCul-01, lactobacillus acidophilus MiuLac-01, lactobacillus casei MiaoTech-01, lactobacillus rhamnosus MiaoTech-11, streptococcus thermophilus MiaoPro-01, bifidobacterium bifidum Miuyo-01, bifidobacterium lactis Miuyo-11, bifidobacterium infantis Miuyo-21 and bifidobacterium breve Miuyo-31, and the multifunctional composite probiotic preparation is prepared from lactobacillus plantarum MiuCul-01, lactobacillus acidophilus MiuLac-01, lactobacillus casei MiaoTech-01, lactobacillus rhamnosus MiaoTech-11, streptococcus thermophilus The composite probiotic preparation is prepared by a new process combining mixed bacteria inoculation, anaerobic liquid culture, solid-liquid separation, solid culture and the like, and the produced composite probiotic preparation is large in thallus quantity and high in activity, and contains metabolites generated by probiotics; the traditional Chinese medicine composition has better effects of resisting helicobacter pylori, reducing blood sugar, coordinating intestines and stomach, enhancing immunity, resisting allergy, reducing fat, losing weight and the like in human gastrointestinal tracts.

The invention relates to the technical field of agriculture and microorganisms, in particular to a strain for preventing and treating greenhouse tomato continuous cropping common diseases and a composite microbial agent thereof. According to the invention, a YJY20-02 strain and YJY19-01 are compounded to obtain the composite microbial agent. After the compound microbial agent is applied, the fieldplanting is easy, the drug resistance is not easy to generate, and the compound microbial agent has obvious bacteriostatic activity on tomato continuous cropping germs, and the bacteriostatic activity is higher than the effect of single use of strains. The strain not only solves the practical problems of unstable prevention and treatment and weak persistence of a single biocontrol strain, but also has a good prevention and treatment effect on common tomato continuous cropping germs, can quickly recover and stabilize flora balance in soil, is a biocontrol strain which is high in tomato continuous cropping common disease prevention and treatment efficiency and environment-friendly, and is wide in application prospect and wide in application prospect. The invention also lays a foundation fordeveloping and developing a biocontrol combined preparation for effectively controlling tomato continuous cropping disease.

The invention provides pig-source lactic acid bacteria capable of improving growing performance of piggies and a prebiotic combination product. A preparation method of the pig-source lactic acid bacteria comprises the following steps of (1) separating bacterial strains from fresh pig manure, and performing identification; (2) inoculating agarslant-preservation target bacteria species to a liquid shake flask culture medium, and performing vibrating to a logarithmic phase; (3) performing inoculating in a fermentation tank, and performing culturing to a logarithmic growth phase; and (4) adding asuspending agent to the fermentation tank, so as to make the pig-source lactic acid bacteria microbial inoculum. The prebiotic combination product consists of the pig-source lactic acid bacteria microbial inoculum, clostridium butyricum, probiotic inulin, and probiotic fructo-oligosaccharide. According to the prebiotic combination product disclosed by the invention, the pig-source lactic acid bacteria microbial inoculum is adopted, so that the diarrhea rate of pigs can be reduced; and combined prebiotics can increase the growth rate of the pigs, the feed conversion rate is reduced, and in thefeeding process of the pigs, the usage amount of antibiotics is reduced.

The invention discloses a method for establishing an autism rat model by applying a fecal bacterium transplantation method. The method is characterized by specifically comprising the following steps of preparation of a fecal bacterium solution, wherein feces derived from patients clinically diagnosed as childhood autism is prepared into the fecal bacterium solution; establishment of the model, wherein the fecal bacterium solution is injected into a pregnant rat by adopting a clysis method, and then the pregnant rat gives birth to newborn mice, namely, autism model rats. The disclosed method has the advantages that the repeatability is strong, the success rate is high, the death rate is low, the symptoms of the model are more typical and more lasting, the cost is low, and the method has practical value and significance.

A high-yield cultivation method of shiitake mushroom or Huagu-type shiitake mushroom based on branch cut-logs comprises: 1) soaking branch cut-logs, grown for half month after Beginning of Winter, in10% lime water for 1 min, and using the sides floated on water as male sides and the opposite sides as female sides; 2) taking out the branch cut-logs, and piling them for 3-4 months with their male sides facing down; 3) inoculating a strain to the piled branch cut-logs, wherein a formula of the inoculated strain includes 87.5-94.5% of sawdust, 5-12% of bran, and 0.5-1.2% of gypsum powder; 4) after the strain attaches for 1.5 years, allowing fruiting, spraying with atomized water at the low temperature of 3-5 DEG C with a temperature difference of 10 DEG C and above, and keeping stuffiness after spraying until few mushrooms occur; 5) transferring to a greenhouse for the purpose of natural growth, and controlling the humidity of the greenhouse to 20-60%. The method of the invention has theadvantages that contamination risk, the yield and quality issues due to fruiting management level, and potential hazards of food safety can be avoided, the yield is higher, and the quality is better and more stable.

The invention discloses a preparation method of a compound microbial bacterium agent capable of effectively inducing formation of agilawood. The compound microbial bacterium agent is characterized by being selected and prepared sequentially by the following technological processes: extracting a compound microbial community, conducting enrichment culture, conducting centrifuging and collecting, conducting freeze-drying in a vacuum environment, promoting formation of the agilawood and conducting comprehensive analysis on an impregnating capacity. The compound microbial community provided by the invention can effectively induce the generation of the agilawood from agilawood plants on inoculation sites; and in addition, problems of existing single-fungus inducing which is low in agilawood yield and poor in agilawood quality, fails to meet a market demand and the like can be successfully solved.

The invention discloses a strain of pseudomonas migulae and application thereof in control of luffa root-knot nematode. The pseudomonas migulae strain YT1001 has been deposited in the China General Microbiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15214; the pseudomonas migulae strain in the invention is easy to colonize around the root system of the luffa, and has a good effect on control of luffa root-knot nematode, with the control effect of 57.17% after 14 days after the last application; from growth of the luffa, the pseudomonas migulae strain YT1001 has an obvious effect on promoting luffa growth, and is safe and reliable without risk of damage.

The invention discloses trichoderma asperellum. The strain is collected in the CGMCC on January 22, 2018, with the biological collection number of CGMCC NO.15266. The trichoderma asperellum obtained by screening and separation is excellent in tolerance and adsorption capacity for various heavy metals, particularly for lead; the trichoderma asperellum is easy to colonize and survival in heavy metal-polluted soil, particularly in lead-polluted soil, and is suitable for microbial repairing for the soil.

The invention relates to the field of biological fertilizers, in particular to a special biological source bacterial fertilizer system for cherries and a use method thereof. Agricultural residues areinnovatively used as raw materials; multiple fermentation process is adopted, the biological source bacterial fertilizer system special for the cherries and the using method thereof are provided, production of solid biological source bacterial fertilizer and liquid biological source bacterial fertilizer special for the cherries is achieved, the production and using method is safe, efficient and convenient, the cherry yield and quality can be improved through matched use of the solid biological source bacterial fertilizer and the liquid biological source bacterial fertilizer, soil can be improved, and fertilizer and resource waste is greatly reduced.