Method for establishing autism rat model by applying fecal bacterium transplantation method

Abstract

The invention discloses a method for establishing an autism rat model by applying a fecal bacterium transplantation method. The method is characterized by specifically comprising the following steps of preparation of a fecal bacterium solution, wherein feces derived from patients clinically diagnosed as childhood autism is prepared into the fecal bacterium solution; establishment of the model, wherein the fecal bacterium solution is injected into a pregnant rat by adopting a clysis method, and then the pregnant rat gives birth to newborn mice, namely, autism model rats. The disclosed method has the advantages that the repeatability is strong, the success rate is high, the death rate is low, the symptoms of the model are more typical and more lasting, the cost is low, and the method has practical value and significance.

Description

technical field [0001] The invention relates to the technical field of establishing an animal autism model, and more specifically relates to a method for establishing an autism rat model by using feces and bacteria transplantation. Background technique [0002] At present, autism spectrum disorder (ASD) is a relatively serious neurodevelopmental disorder that mostly occurs in infants and young children. Its typical features are communication impairment and imagination impairment, social dysfunction, and stereotyped repetitive interests and behaviors. , accompanied by motor coordination disorders. According to the latest data from the Centers for Disease Control and Prevention in the United States, the incidence of ASD has increased from 1 / 68 in 2014 to 1 / 59 by 2018. It has been reported in the literature that there are about 4 to 10 million autistic patients in my country, of which about 1 to 3 million are children. At this stage of research, it appears that autism spectru...

AI Technical Summary

Problems solved by technology

However, due to the complex etiology of ASD and the unclear pathogenesis, there is also a lack of strict animal models for experimental research.

From the analysis of the research status at home and abroad, in recent years, there are many ways to establish animal models of ASD and new progress has been made, but there are still some defects, such as time-consuming model preparation, overly complicated operations, poor reproducibility, and high research costs. And scientific research equipment has strict requirements, and cannot be widely promoted, etc.

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