Method for establishing autism rat model by applying fecal bacterium transplantation method

A technology of fecal bacteria transplantation and rat model, which is applied in animal husbandry and other fields, can solve problems such as strict requirements for scientific research equipment, long time-consuming model preparation, and unclear pathogenesis, so as to ensure filtration rate, easy implementation, and low mortality Effect

Active Publication Date: 2019-08-30
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

However, due to the complex etiology of ASD and the unclear pathogenesis, there is also a lack of strict animal models for experimental research.
From the analysis of the research status at home and abroad, in recent years, there are many ways to establish animal models of ASD an

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  • Method for establishing autism rat model by applying fecal bacterium transplantation method
  • Method for establishing autism rat model by applying fecal bacterium transplantation method
  • Method for establishing autism rat model by applying fecal bacterium transplantation method

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Embodiment 1

[0047] Embodiment 1 of the present invention discloses a method for establishing a rat model of autism by using fecal bacteria transplantation, which specifically includes the following steps:

[0048](1) Preparation of feces liquid:

[0049] After weighing, put the fresh feces of autistic patient donors in a sterile mixer, add sterile saline according to the sterile saline: feces = 5mL / g and stir; pass the feces through the apertures of 2.0mm and 1.0mm in turn , 0.5mm and 0.25mm four kinds of sterile stainless steel filter screens to filter the fecal juice;

[0050] B. Centrifuge the filtered fecal juice at 3000r / min for 15min, discard the supernatant, add normal saline to the original volume, mix well and then centrifuge, repeat this step 3 times to obtain the residue;

[0051] C. After weighing the residue, suspend it in normal saline to obtain 5g / mL fecal bacteria liquid.

[0052] The total treatment time is ≤60min. The fresh fecal bacteria liquid is directly transplante...

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Abstract

The invention discloses a method for establishing an autism rat model by applying a fecal bacterium transplantation method. The method is characterized by specifically comprising the following steps of preparation of a fecal bacterium solution, wherein feces derived from patients clinically diagnosed as childhood autism is prepared into the fecal bacterium solution; establishment of the model, wherein the fecal bacterium solution is injected into a pregnant rat by adopting a clysis method, and then the pregnant rat gives birth to newborn mice, namely, autism model rats. The disclosed method has the advantages that the repeatability is strong, the success rate is high, the death rate is low, the symptoms of the model are more typical and more lasting, the cost is low, and the method has practical value and significance.

Description

technical field [0001] The invention relates to the technical field of establishing an animal autism model, and more specifically relates to a method for establishing an autism rat model by using feces and bacteria transplantation. Background technique [0002] At present, autism spectrum disorder (ASD) is a relatively serious neurodevelopmental disorder that mostly occurs in infants and young children. Its typical features are communication impairment and imagination impairment, social dysfunction, and stereotyped repetitive interests and behaviors. , accompanied by motor coordination disorders. According to the latest data from the Centers for Disease Control and Prevention in the United States, the incidence of ASD has increased from 1 / 68 in 2014 to 1 / 59 by 2018. It has been reported in the literature that there are about 4 to 10 million autistic patients in my country, of which about 1 to 3 million are children. At this stage of research, it appears that autism spectru...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 杨丽萍亓照耀袁海燕刘娟翟林林曹赟吕梦珂王洪刚王启阳
Owner HENAN UNIV OF CHINESE MEDICINE
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