Pseudomonas migulae and application thereof in control of luffa root-knot nematode

A technology of Pseudomonas and loofah roots, applied in the direction of application, pest control, nematicide, etc., to achieve the effect of promoting the growth of loofah, promoting the growth effect, and good effect

Inactive Publication Date: 2018-11-23
山东济宁邦尔利工贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the use of Pseudomonas to control nematodes

Method used

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  • Pseudomonas migulae and application thereof in control of luffa root-knot nematode
  • Pseudomonas migulae and application thereof in control of luffa root-knot nematode
  • Pseudomonas migulae and application thereof in control of luffa root-knot nematode

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Embodiment 1

[0029] 1. Isolation and purification of Pseudomonas migulae (P.migulae) YT1001

[0030] Pseudomonas migulae (P.migulae) YT1001 of the present invention is obtained from the wetland mud at the mouth of the Dagu River in Jiaozhou Bay, Qingdao, using the dilution plate method and the plate marking method to separate and obtain, and the separation method is:

[0031] (1) Isolation of strains

[0032] The sample was collected from the mud sample at the estuary of the Dagu River in Jiaozhou Bay, Qingdao on May 11, 2014. Weigh 1g of the sample in 99mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, and then place it in a water bath at 60°C Incubate in medium for 30min, take 100μL 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are picked and streaked on the LB medium plate, and observed regular...

Embodiment 2

[0053] 1. Fermentation process of Pseudomonas mieteri strains

[0054] NYBD liquid medium, the formula is: beef extract 8g, yeast extract 5g, glucose 10g, sodium chloride 4.5g, water 1000mL, pH adjusted to 7.2-7.4.

[0055] Solid medium formula: solid material and inorganic salt, the mass ratio of described solid material and inorganic salt is 98.8: 0.2; Described solid material is counted as wood flour by mass ratio: corn flour: soybean meal flour: bran=38:30 : 22: 10; the composition and weight ratio of the inorganic salt are: potassium dihydrogen phosphate 22%, magnesium sulfate 8%, zinc sulfate 3%, ammonium sulfate 13%, light calcium carbonate 54%. Preparation method: adding an appropriate amount of water (10-15 times the quality) to dissolve the inorganic salt to obtain an inorganic salt solution; mixing the inorganic salt solution with the solid material, and adding water to finally adjust the water content of the solid medium to 40-60%, so that the Hold the solid mediu...

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Abstract

The invention discloses a strain of pseudomonas migulae and application thereof in control of luffa root-knot nematode. The pseudomonas migulae strain YT1001 has been deposited in the China General Microbiological Culture Collection Center (CGMCC) in January 15, 2018 with the Accession No. of CGMCC No. 15214; the pseudomonas migulae strain in the invention is easy to colonize around the root system of the luffa, and has a good effect on control of luffa root-knot nematode, with the control effect of 57.17% after 14 days after the last application; from growth of the luffa, the pseudomonas migulae strain YT1001 has an obvious effect on promoting luffa growth, and is safe and reliable without risk of damage.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. Specifically, the present invention relates to a strain of Pseudomonas mierii capable of effectively preventing and treating the root-knot nematode of loofah and its application, and a biological control agent prepared by using the Pseudomonas mierei. Background technique [0002] Plant-parasitic nematodes are an important class of plant pathogens, causing an estimated $157 billion in global agricultural economic losses annually. There are four common types of root-knot nematodes, namely, southern root-knot nematode (Meloidogyne incognita), northern root-knot nematode (M.hapla), Javan root-knot nematode (M.javanica) and peanut root-knot nematode (M.arenaria). According to incomplete statistics, there are more than 3,000 host plants of root-knot nematodes, including economic, food, fruit trees, vegetables, flowers and other crops. Among them, the plants such as Solanaceae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P21/00A01G13/00C12R1/38
CPCA01G13/00A01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 张茂印牛赡光张淑静殷碧秋刘殷
Owner 山东济宁邦尔利工贸有限公司
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