562 results about "Meloidogyne incognita" patented technology

The invention discloses a method for producing fungi in a strain of plant and the application. Fungi which is produced in plants is Acremonium strictum HND5 CGMCC NO.2192. The bacterial strain and metabolite thereof can inhibit pathogen fungi activity in broad spectrum, can produce activity volatility natural gas chemical compound which can inhibit the activity of pathogenic fungi, the bacterial strain can parasitize banana root-knot nematode, metabolite can poison banana root-knot nematode, the bacterial strain can be widely used in the aspects of biologically preventing and curing plant diseases and pests, arm-shaped grass production, and in the aspects of fungi modified and breeding material.

The invention discloses a cultivation method capable of controlling tomato root knot nematode. The cultivation method includes that innovations are made on double-concave-structure cultivation ridges, a sunlight greenhouse tomato special planting mode is set, organic combining of biological trapping and biological fumigating is realized, and the objective of controlling damage of tomato root knot nematode is achieved. No chemical pesticide is used in the cultivation method, so that tomato root knot nematode can be effectively prevented and controlled, root knot index of tomatoes is lowered greatly, yield of the tomatoes is increased, and the cultivation method is low in energy consumption and meets the low-carbon concept.

The invention discloses a styryl-containing 1,3,4-oxadiazole thioether compound. The structural general formula (I) is as follows: R1 is hydrogen, 4-methoxyl, 4-chlorine, 4-fluorine or 4-bromine; R is methyl, ethyl, benzyl, 2,5-dichlorobenzyl, 4-fluorobenzyl, 4-hlorobenzyl, 2-methylbenzyl, 4-nitrobenzyl, 1,1,2-trifluoro-butenyl or the like. The styryl-containing 1,3,4-oxadiazole thioether compound has activities of killing citrus nematode and meloidogyne incognita chitwood. (The formula (I) is as shown in the description.).

The invention discloses a compound bacterium for preventing and treating root-knot nematode and a preparation method thereof. The compound bacterium for preventing and treating root-knot nematode is prepared by the following steps: carrying out high-density cultivation on raw material strains of bacillus thuringiensis, pseudomonas fluorescens, bacillus subtilis, streptomycete, aspergillus niger, paecilomyces lilacinus and fusarium oxysporum, inoculating activated and cultivated strains in a fermentation tank and implementing fermentation and extended cultivation; dehydrating and drying various single strains obtained from extended cultivation to obtain resting microorganism dry powder; and mixing 10-20 parts of bacillus thuringiensis, 5-10 parts of pseudomonas fluorescens, 10-20 parts of bacillus subtilis, 10-20 parts of streptomycete, 10-15 parts of aspergillus niger, 10-25 parts of paecilomyces lilacinus and 8-15 parts of fusarium oxysporum to obtain the compound bacterium for preventing and treating root-knot nematode. The compound bacterium disclosed by the invention is used for preventing and treating root-knot nematode; and compared with an existing product, the compound bacterium is environment-friendly, harmless and non-toxic to a human body and good in control efficiency.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to a nematoid-killing composition containing abamectin and paecilomyces lilacinus, which is characterized by comprising abamectin and paecilomyces lilacinus; 100g of nematoid-killing composition is prepared by the following components of 0.1-5g of abamectin, 0.1-1 billion live spores/g of paecilomyces lilacinus, 1-10g of stabilizing agent, 1-90g of solvent and 10-90g of assistant, wherein the assistant is any one or the combination of any two of attapulgite, kaoline, sepiolite, light calcium and kieselguhr and is prepared into a granular formulation or suspending agent or wettable powder; the stabilizing agent is any one or the combination of any two of castor oil, soybean oil, epoxidized soybean oil, propylene glycol, methyl oleate, ethylene glycol, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA); and the solvent is any one of dimethylbenzene, trimethylbenzene and dimethylformamide. The nematoid-killing composition is used for controlling the knot nematode and has better control effect on the knot nematode.

The invention relates to a soilless seedling culturing substrate for resisting diseases and root nematode of plants and a production method thereof. In the invention, organic wastes such as straws are taken as raw materials, inoculated with zymogen according to certain proportion, subjected to moisture content regulation, evenly mixed and stacked for primary fermentation; when the temperature of a pile reaches 60 DEG C, the pile is turned; the pile is turned and oxygen and moisture content are supplemented every seven to ten days afterwards; after the pile is turned for three times, when the temperature is lower than 35 DEG C, the pile is inoculated with a bio-antagonist for secondary fermentation, turned every three to four days and kept at a temperature of between 35 and 40 DEG C; and after 15 to 20 days, the seedling culturing substrate containing the 10 bio-antagonist is obtained. The seedling culturing substrate has chemiotaxis for interfering pathogenicbacteria and nematode, can reduce infestation chance of the pathogenicbacteria and nematode on the root surface, and has double effects of resisting phytophthora disease and root nematode disease through the secondary fermentation and microorganism activation technology.

The invention relates to a method for artificial culture and propagation of plant root-knot nematodes and for species preservation. The propagation and preservation of the root-knot nematodes in a lab depend on host plants all the time; the invention places a relatively small amount of root-knot nematode ovums or 2nd instar larvas in a particular dual culture medium rather than host roots and performs a upside-down culture under conditions of constant temperature and hermetic closure, the root-knot nematodes ingest, grow and propagate inside the culture medium and a large amount of nematomorphas can be obtained in a short term; additionally, a culture plate is timely transferred to be located under cold conditions, and dish change and subculture are implemented once within a half year so that the propagation and preservation processes of the root-knot nematodes are carried out without the host plants. The inventive advantages lie in: saving the culture program of the root-knot nematodes to host the plants without losing the infectivity of the root-knot nematodes on sensitive host plants; the employed materials have low costs and the experiment method is easy in operation; the invention provides a simple, convenient and fast propagation method for the species preservation of the root-knot nematodes, in particular for a large number of nematomorphas as required in the related researches of the root-knot nematodes.

The invention discloses a new method for controlling vegetable root knot nematode, which is characterized in that one or two from chitin, aropaz, Paecilomyces lilacinus and nematicidal pioneer is/are selected to process a seedbed in 1 to 2 weeks before the field planting of vegetables; 1.0Kg to 2.0Kg /mu (1 acre equals to 6.07 mu) of Fosthiazate and 20Kg to 60Kg/mu of harmonia mundi are mixed to process soil before the field planting; after the field planting, a biological agent is matched and used; the method is convenient to be used, is safe and effective, not only can effectively control the root knot nematode, but also can promote the growing of the vegetable plants, has the double effects of pesticide and fertilizer and can lighten the environment pollution brought by the use of the pesticide in large dosage; in the early period, the numbers of the eelworms in the soil are reduced by the mechanical eelworm pesticide of Fosthiazate; the stress resistance of the vegetables are increased by the chitin and the pesticide-fertilizer harmonia mundi; during the later period of the field planting, the numbers of the eelworms remained in the soil are controlled by using any of the biological agent of chitin, the aropaz, the Paecilomyces lilacinus and the nematicidal pioneer to irrigate the root, thus achieving the effect of controlling the vegetable root knot nematode by the cooperation of biology and chemistry and having very high popularization value.

The invention relates to a suspending agent sterilizing two-spotted spider mite Bacillus thuringiensis, belonging to the field of biological pesticides. The suspending agent is prepared by mixing fermentation liquid of the Bacillus thuringiensis BtR05 with a surfactant, an antiseptic agent, a suspending auxiliary agent and a thickening agent, wherein, the suspending auxiliary agent is ammonium sulphate. The invention also provides preparation methods and uses of the suspending agent, the suspending agent prepared by the BtR05 bacterium and the fermentation liquid of the bacterium is quite poisonous against two-spotted spider mite, American white moth, diamond-back moth, chive maggot, grub, maggot galerucella and Meloidogyne incognita, which is an efficient and broad-spectrum biological pesticide.

The invention relates to an antibiotic streptomyces, fermentation product of which can inhibit broad spectrum fungus and protozoan, and field experiment can control crop fungus plant disease and root-knot nematode. The antibiotic streptomyces has good effect, no pollution nuisance and no residue. The antifungal agent has extremely low hazardness for the people and good performance and development prospect for medical and agricultural use. The invention is named as SIM001 antibiotic streptomyces which is Streptomyces antibioticus subspecies xi an, and is preserved as patent in the depositary institution appointed by patent office of state intellectual property office; the preservation data is April 14th, 2005, the name of the depositary institution is CGMCC, and the number of preservation is CGMCC No.1349. The whole cell hydrolysate of the antibiotic streptomyces contains L, L-DAP (Diaminopimelic acid) and no characteristic glucide; the cytoderm belongs to I type and glucide type C; one of the main antibacterial materials is polyene macrocyclic ketolide which belongs to the broad spectrum antifungal substance.

The invention provides a functional bactericide for preventing and treating root-knot nematode.The functional bactericide comprises an azotobacter bactericide, a biocontrol bacterium bactericide and a carrier, and the mass ratio of the azotobacter bactericide to the biocontrol bacterium bactericide to the carrier is (1-2.5):(1.5-5):1.The azotobacter bacterium bactericide contains nodule bacteria and azotobacter chroococcum, and the living bacterium count ratio of nodule bacteria to azotobacter chroococcum is (1-100):(1-200).The biocontrol bacterium bactericide contains paecilomyces lilacinus, verticillium chamydosporium, bacillus subtilis and bacillus cereus, and the living bacterium count ratio of paecilomyces lilacinus to verticillium chamydosporium to bacillus subtilis to bacillus cereus is (1-100):(1-100):(1-100):(1-100).The living bacteria count of the functional bactericide is larger than 0.2 billion/g.When applied to fertilizer, the functional bactericide can improve the disease preventing effect and the growth promoting effect of microbial fertilizer, guarantee nutrition supply of microbial fertilizer in the applying process, achieve the effects of preventing and treating root-knot nematode, forming healthy root nodules and promoting plant growth, increase the yield of crops and improve the quality of crops.

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug/ml of cadusafos. The fungus has good application and development prospects.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention relates to an enterolobium cyclocarpum knot nematode loop-mediated isothermal amplification (LAMP) rapid detection method and applications. The method comprises the following steps: designing 5 LAMP primers: MEF3, MEB3, MEFIP, MEBIP and MELB in a sequence conversed domain according to an enterolobium cyclocarpum knot nematode ITS sequence of the clone sequencing; configuring a LAMP reaction system; detecting isothermal amplification and amplification product color developing of the enterolobium cyclocarpum knot nematode through extracting the DNA of the enterolobium cyclocarpum knot nematode, thus rapidly detecting the enterolobium cyclocarpum knot nematode. The detection method provided by the invention has strong specific, high sensitivity, low cost and simple operation, and has high application value in the aspects of spot rapid detection and the early-stage diagnosis of the enterolobium cyclocarpum knot nematode.

The present invention relates to a biological control strain AR156 for preventing and treating root-knot nematodes of greenhouse vegetable, belonging to plant protection technical field. The bacteria is bacillus sp., which is cultivated in PDA culturing liquor under 37 degree and 180rpm, then is centrifuged 10min under 6000rpm. Picking wet bacteria and bacteria storage liquid is prepared into bacteria agent according to 1:40 (g/ml)proportion. The living bacteria concentration is 1*109-1*1012cfu/ml.The effective rate of the bacteria agent amount to 58-75% for preventing root-knot nematodes of cucumber, towel gourd, tomato etc, and yield increase more than 30-50%. The bacteria agent can also treat wilt, epidemic disease and soil hardening. The bacteria agent can be sprayed, composted and irrigated to root.

The organic modifier for preventing and controlling vegetable rot knot nematode disease consists of dried wheat straw decaying process in 90-99 wt% and Paecilomyces lilacinus as biological germicidal agent in 1-10 wt% mixed homogeneously. The organic modifier may be applied in preventing and controlling vegetable rot knot nematode disease effectively. It has no toxicity to vegetable, high efficiency, high safety and environment friendship.

The invention relates to a tomato grafting method, which comprises the following steps of: sowing stock seeds; sowing cions and tomato seeds when the height of stock seedling is 4-6 cm; transversely cutting off top interior leaf at a position 4-5 cm above the lignification position of the root of the stock seedling, vertically cutting downwards from the middle position of the stock stem to a depth of 0.4-0.5 cm, transversely cutting at the depth of 0.5 cm to remove half of the stock stem with an incision angle of 90 degrees; removing root at a position 1.4-1.6 cm below the stem leaf of the cion seedling, vertically cutting upwards from the middle position of the cion stem to a depth of 0.5 cm, transversely cutting at the depth of 0.5 cm to remove half of the cion stem with an incision angle of 90 degrees; butting the incision of the stock and the incision of the cion and fixing with a fixture. Since the incisions of the stock and the cion are right angles, the contact area of the joint is large, the joint can be healed up completely, the incisions of the stock and the cion have strong affinity, and the survival rate reaches more than 93 percent. After grafting, a produced tomato variety has remarkably enhanced root-knot nematode disease resisting capability and TY virus resisting capability, and the yield of tomatoes is obviously improved.

The invention discloses a bacillus subtilis bacterial strain and application thereof. The strain is bacillus subtilis Bs-1, is preserved in China Microbiological Collection Management Committee General Microbiology Center, and has a preservation number of CGMCC NO.12364. The invention further discloses application of a biological agent prepared by the bacillus subtilis Bs-1 to preparation and treatment of root-knot nematode. In the invention, bacillus subtilis is separated from soil, has an excellent control effect on root-knot nematode, is easy for colonization in the soil, and is beneficial to early prevention and control of the root-knot nematode hazard; the bacterial strain has a huge application potential for solving the problem of prevention and treatment of the root-knot nematode, and is helpful for implementing high yield, safety and pollution-free production of vegetables; fermentation liquor of the bacillus subtilis Bs-1 bacterial strain reaches a fatality rate of 100% on root-knot nematode, reaches a prevention and treatment effect of 76.9% in a greenhouse, has the good and stable prevention and treatment effect, and is suitable for agricultural production requirements.

The invention relates to a cultivation of epiphyte that has plant infestation wireworm poisoning function and the metabolite manufacture method and the application. The strain of the epiphyte is Asipergillus. niger snf0407009 and the conserving code is CGMCC No.1714. It is made up from normal liquid fermenting and cultivating, and its metabolite has high poisoning function to plant infestation wireworm, especially root wireworm. The influence to the Meloidogyne incognita has been measured indoor. Different thickness fermenting liquid would have obviously influence to Meloidogyne incognita. One time fermenting liquid has the similar function as 10ug/mL aldicarb. It has good application prospect.

The invention belongs to the field of pesticide and fertilizer compounding, and relates to a pesticide fertilizer special for root-knot nematode prevention. The pesticide fertilizer is prepared as follows: according to the infestation habits of root-knot nematodes and the fertilizer requirement law of crops, selecting the pesticide active ingredients ivermectin and/or fosthiazate, selecting one or more kinds of materials capable of improving the resilience of crops, such as chitin, humic acid and seaweed fertilizer, selecting and adding major element fertilizer, secondary element fertilizer and minor element fertilizer, and adding a carrier, so as to obtain the pesticide fertilizer special for root-knot nematode prevention. The ingredients in the pesticide fertilizer match with the infestation habits of root-knot nematodes and the nutrient absorption law of crops, so as to improve the pesticide and fertilizer utilization rate, reduce the investment costs, improve the soil environment, and lessen the agricultural non-point source pollution.

The invention relates to granules containing monosultap and thiamethoxam. The granules containing monosultap and thiamethoxam comprises, by weight percent, 0.5%-15% of monosultap, 0.01%-10% of thiamethoxam, 0.1%-10% of chitin, 1%-15% of auxiliary, 0.5%-10% of binder, and the balance of carrier. The granules containing monosultap and thiamethoxam are used for controlling soil insects, nematodes and overground insects for crops such as sugarcane, banana, corn and peanut, have evident synergistic effect, and are fine in controlling action, long in duration and wide in insecticidal spectrum. Preventive effect of the crops against the root nematodes and pathogenic bacteria can be improved evidently, rhizome development and plant growth of crops are promoted, crop resistance is enhanced, and crop yield and quality are increased.

The invention discloses a botanical pesticide for vegetable root knot nematodes, which comprises, by weight, tea saponin 30-35 parts, nicotine 16-20 parts, ricinoleic acid 20-30 parts and brucine12-18 parts. The botanical pesticide uses the tea saponin as a main active ingredient and uses the nicotine, the ricinoleic acid and the brucine1 as auxiliary ingredients and can effectively kill and restrain target nematodes.

The invention relates to preparation and application technology for a waxy bacillus suspension agent. The suspension agent is characterized by comprising the following components of: usually 1 to 1,010 bacterial colonies of living body waxy bacillus per milliliter, 1 to 25 weight percent of dispersing agent, 0.1 to 5 weight percent of thickening agent and the balance of water. The waxy bacillus suspension agent can be used for efficiently preventing and controlling root-knot nematodes of greenhouse vegetables such as watermelons, balsam pears, west melons, cucumbers, tomatoes, towel gourds, celeries and the like and outdoor vegetables. The using method comprises the following steps of: (1) after applying farmyard manure on a soil surface, diluting 1,000 milliliters of the waxy bacillus suspension agent by using 40 to 50 kilograms of water, uniformly spraying the diluted suspension agent on the soil surface, and turning soil; and transplanting crops after 3 to 10 days, wherein the effect of preventing and controlling the root-knot nematodes of the vegetables can reach 70 to 90 percent; and (2) after conventional fertilization of the soil, diluting 6,000 milliliters of the waxy bacillus suspension agent by using water until the diluted volume is 40 to 50 times the original volume of the suspension agent and irrigating roots by using the diluted suspension agent which serves as root-fixing water when transplanting the crops. The waxy bacillus suspension agent can efficiently prevent and control the root-knot nematodes of the vegetables.

A method for controlling Angelica sinensis root-knot nematode by marigold interplanting belongs to the field of controlling damage by plant parasitic nematodes, and particularly relates to a method for controlling Angelica sinensis root-knot nematode by changing the Angelica sinensis cropping system. The method includes the steps: transplanting marigold to two sides of each ridge (having a bed width of 1.7m) with a plant spacing of 30cm twenty days before Angelica sinensis transplanting; and when 20 days are past, transplanting four plants of Angelica sinensis per row of marigold with a plant spacing of 28cm, and allowing each row of Angelica sinensis to be spaced from marigold by 35cm so as to form 2:4 interplanting of marigold and Angelica sinensis (see attached figures). Compared with single culture of Angelica sinensis, the method can be more effective in root-knot nematode control by more than 75% and also has a composite field increasing effect for both Angelica sinensis and marigold, and land utilization rate is increased by 70%.

The invention discloses an insecticidal composition which comprises an active substance A and an active substance B which are used as effective components, wherein A is tefluthrin, B is one of neonicotine insecticides comprising acetaniprid, imidacloprid and thiamethoxam, and the mass ratio of A to B is 60:1-1:80. The composition of the invention has obvious synergy, can lower the consumption of the effective components and reduce the insecticide applying times, is used for controlling various agricultural pests, and is especially suitable for controlling scarab larvae, flea beetle larvae androot-knot nematode.

The invention discloses a paecilomyces lilacinus space mutation mutant strain Sd-m-16 and a microbial preparation and an application thereof. A paecilomyces lilacinus strain 20-7 and a Shandong strain are carried on the Shenzhou 8 spacecraft for space mutation, and a non-carried original strain is used as a contrast to compare the variability of the space-carried strain on aspects of configuration, pigment change, growth speed, sporulation quantity, pathogenicity and the like to screen a mutant strain with improved growth performance and greatly improved pathogenicity on root-knot nematode eggs. The screening on the growth speed, sporulation quantity and pathogenicity of the space-carried strain indicates that wide positive mutation has been generated on such aspects of the space mutant strain Sd-m-16 of the Shandong strain as growth property, pathogenicity to root-knot nematode eggs and the like; particularly, the pathogenicity of the mutant strain is greatly improved in comparison with that of the original strain. The invention further provides a microbial preparation containing the space mutant strain and an application of the space mutant strain in biological prevention and cure of root-knot nematodes.

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.